n****0
发帖数: 696 | 1 想区分开只有14bp差的两个DNA片段，片段大概140-160bp左右，用高浓度的high
resolution agarose，跑上3、4个小时可以分开么？如果可以的话请推荐个牌子，
sigma的怎么样？
或者还有其他什么高招？多谢！ |
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b****r
发帖数: 17995 | 2 100多bp的片段差14bp，普通agrose做硬一点跑慢一点冷一点毫无压力 |
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i*u
发帖数: 299 | 3 "Buy in April, Sell in May" isn't effective so far this year, stocks
continued to stay under water, where the S&P500 is returning -2.63% since
April started. On the other hand, the bond buyers are enjoying a cheerful
time with the weak economic releases recently, the Treasury yields curve
fell sharply where the 2yr and the 10yr went down 14bps and 37bps
respectively. Aside from the technical, first quarter corporate earnings are
reporting strong numbers as Alcoa, Google, JP Morgan and Wells Farg... 阅读全帖 |
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s*****g
发帖数: 87 | 4 不太可能，CRISPR的特异识别序列只有14bp，很容易target到其他genomic region
TALEN的特异识别序列有30-60bp,远远高于CRISPR |
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N******o
发帖数: 3053 | 6 Invitrogen agarose-1000高浓度，水浴加热配制，可以分开。 |
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n****0
发帖数: 696 | 7 谢谢！我发现普通的agarose也可以，80V跑了2h，大概带有点糊 |
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a******8
发帖数: 56 | 9 当年用4% 低熔点agarose gel 分过9bp缺失的条带，原长109bp |
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s*****i
发帖数: 315 | 11 不需要回收片段的话，可以用12%的 Acrylamide-urea page， 效果非常好 |
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