l**********1
发帖数: 5204 | 2 Samtool file
flag inside sam file
SEQanswers - Bioinformatics — I got a read aligned as below: ~ HWI-1KL138:2
:2105:12847:125331#GCCAATGCCAAT 161 chr1 12036 1 74M = 12645 1188
CTGTGCCAGGGTGCAAGCTGAGCACTGGAGTGGAGTTTTCCTGTGGAGAGGAGCCATGCCTAGAGTGGGATGGG
hhhhhhhhhhhghhhhhhhhhhhhhhhhhheffffdffffhhhghhehefhhhhhghhfhfffWdbfffbffff
NM:i:0 NH:i:3 CC:Z:chr15 CP:i:102519061 HI:i:0 ~ The flag here is 161=128+32
+1. 128 means 2nd pair; 32 means mated reverse strand; 1 means paired read.
I am wondering why th... 阅读全帖 |
|
