g***1
发帖数: 24 | 1 Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences! |
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y******8
发帖数: 1764 | 2 4-5% resolving gel and transfer buffer without methanol. |
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i********e
发帖数: 50 | 4 I use 6% separation gel and transfer without methanol for one hour at 100 V
. My protein is 550 KDa. |
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s******r
发帖数: 2876 | 5 invirogen 有 Tis-acetate gel，据广告适合大蛋白。
Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences! |
|
i*********t
发帖数: 78 | 6 add SDS in trnasfer buffer. |
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p********t
发帖数: 411 | 7 Are you labeling Ryanoding Receptor?
V |
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