d*****3
发帖数: 65 | 1 俺用invitrogen 4-12%，可以看到两条带，但很接近。有没有更好的体系来跑，俺想跑
完胶，然后做in-gel digestion. 非常感谢。 |
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j*******8
发帖数: 933 | 4 Use 7.5/100 gel. I'm very confident with it! |
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d****h
发帖数: 4291 | 5 自己配胶啊
从12%，往上试试，用不了几下你就得到分的好的了 |
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m**z
发帖数: 787 | 6 another thought: you may not have to separate them for in-gel digestion
followed by MS-MS. Of course this depends on your purpose... |
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z****g
发帖数: 3340 | 7 额得神。Tricine-SDS page is perfect for such separation. good luck! |
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q******g
发帖数: 3858 | 9 我也是这样。如果两个蛋白分子量差不多，就换个loading control. GAPDH 37Kd,
TUBULIN50Kd, 差别还是够大。
如果做phosphorylated protein, 我还是建议strip。 |
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