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A***u 发帖数: 3714 | 8 This is SpicyGirl's another ID, the president&CEO of Merry & Marcel Inc.
Please contact me @ a*********[email protected], or call 906-813-0034
Let us do legitimate , real business.
Don't fool the students here and seduce them to risk their life/ career for
you...
It is totally against the USA law and the related regulation to buy/get the
antibody for any facility which has not been closely monitored by the
authority......
作。 |
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a***n 发帖数: 575 | 11 求生物公司的生物试剂,例如抗体。如有能拿到折扣的朋友,请 站内pm我, 寻求合作。
谢谢 |
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A***u 发帖数: 3714 | 19 This is SpicyGirl's another ID, the president&CEO of Merry & Marcel Inc.
Please contact me @ a*********[email protected], or call 906-813-0034
Let us do legitimate , real business.
Don't fool the students here and seduce them to risk their life/ career for
you...
It is totally against the USA law and the related regulation to buy/get the
antibody for any facility which has not been closely monitored by the
authority......
作。 |
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b****3 发帖数: 8 | 34 诚信 寻求生物或 医学 专业 兼职(代购生物试剂)
要求:
1)读生物or 医院专业的人
2)主要的事情是在网上负责采购
3)人必须诚实 守信用
4)主要的品牌为 abcam cst r&d invitrogen sigma ABI等
有兴趣的人,可联系
QQ 574536113 邮箱: 5*******[email protected]
我公司的网站为 http://www.163bio.com |
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j**********t 发帖数: 44 | 35 RT,想求个工作内推。十分感谢!
或者有认识的亲戚朋友在那里工作,也请帮忙联系一下我。
谢谢关注!祝大家元宵节快乐~ |
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no 发帖数: 560 | 36 单抗的话,Roche的非常好,据说AbCam的单抗和多抗都不错,但价钱都很贵,多抗里面cl
ontech自己的多抗就挺不错,不过得是对GFP fulllength的那个,另一个peptide Ab效价
太低,不好用。以上的抗体在WB和IP都很好使。
但是,单抗的问题在于protein folding和epitope exposure,我们有几中GFP的融合蛋白
,蛋白折叠的构象使其不能被GFP单抗IP,甚至不能在WB中被识别,只能用多抗。 |
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D******9 发帖数: 2665 | 38 This is should be very clean. You can also try the one from Abcam(rabbit
anti-V5). |
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s******y 发帖数: 28562 | 39 The one from Abcam is the best for IP.
The one from Roche is good for WB but not very efficient for IP |
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t*x 发帖数: 1065 | 40 我们实验室一直用兔子多抗anti-ubiquitin
可是最近要做ICC,跟另一个兔子抗体一起染,所以想要买个mouse来源的
请大家帮忙推荐一个好用的抗体,尤其是用在ICC比较好的(特异性强,背景低的)
刚才google了一下
IMGENEX, abcam,santa-cruz....很多公司都有,但不知哪个好
请用过的同学不吝赐教。。。
谢谢大家!!^_^
谢谢! |
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s******y 发帖数: 28562 | 41 rabbit polyclonal antiGFP from abcam is the best one I ever used |
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s******y 发帖数: 28562 | 42 Related question: does the anti-GFP from sigma work for denaturing condition?
The one from abcam is not very efficient in denaturing condition when SDS is
present. |
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r****r 发帖数: 379 | 43 他们的business model到没有什么特殊之处,不过在他们这一类技术服务公司中,很少
有开始就设立一个很强大的sales department的,这个是章的独到之处,也是他们能迅
速起步的重要原因。现在就看他们能否技术上有突破了,否则只能向abcam那个模式去
发展了。 |
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h*********r 发帖数: 220 | 44 I see. Abcam 又是什么模式?licensing & 代理? |
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N***P 发帖数: 153 | 45 260mg眼球组织(玻璃体,虹膜,视网膜,睫状体--除了巩膜角膜,其他能取的,都去
了)
按照Abcam的western blot protocol,5mg加300ul,这也太多了吧。而且晶状体里面大部
分是水。 |
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s****9 发帖数: 932 | 46 Abcam anti-lacZ for IHC works well |
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j****g 发帖数: 406 | 47 abcam的一个chicken的应该不错。我们实验室挺多人用 |
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l*****4 发帖数: 267 | 48 同问 GFP用过abcam和invitrogen的都不好用 |
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v***a 发帖数: 1242 | 49 不知道从GeneScript或Millipore买的抗体不好能退吗?比如说拿回refund或者credit
。做western blot可检测出来的蛋白size都不对。第一次买GeneScript的抗体,它有40
ug和200 ug的,我直接买了200 ug的,一下来了4管,我只用了一管就发现不行。试了
好几家公司的,快疯掉了,老板看我买了这么多抗体都不work,要生气了。。。
大家有这类退货的经验吗?就我自己的经验,Santa Cruz的给credit最容易,abcam的
比较难。其它的公司至今还没argue的经验。
谢谢了。 |
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h*******o 发帖数: 4884 | 50 From my experience, millipore's CS is quite good and it is very easy to
replace a non-functional antibody.
Abcam is very notorious and its quality is plunging, almost like santa cruz
now. |
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