l**c
发帖数: 1274 | 1 谢谢包子，你怎么比我还穷？
★ 发自iPhone App: ChineseWeb 7.8 |
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l******3
发帖数: 1478 | 2 咱俩就算穷帮穷了？这不答应大家散尽家产感谢各位了，虽然不多，一份心意。 |
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d**********r
发帖数: 24123 | 3 谢谢包子
你这个有2/3赚了，应该是有点搞头的。
哥们太惨了。
周一快出，应该还不全赔。不过，也不是破产，Fnma的情况还是有可能的。 |
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l******3
发帖数: 1478 | 4 谢谢州长的建议。周一一开盘就把剩下的卖了，然后看机会。这个情况以前没经历过，
说好了6/6 delist, 这几天的情形很特别。 |
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d**********r
发帖数: 24123 | 5 好惨啊。
都0.85了，不知道那位$2入的哥们是不是还hold住呢 |
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n*********e
发帖数: 25274 | 6 垃圾股不能buy&hold很久,而且一般touch过一次,赚个50%后,就不要再碰它第二次,这是
经验... |
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l**********r
发帖数: 4612 | 8 【 以下文字转载自 Military 讨论区 】
发信人: TravisBickle (Travis), 信区: Military
标 题: 看这篇NYT以后affirmative action要按收入财产鉴别
发信站: BBS 未名空间站 (Sat Oct 13 22:19:34 2012, 美东)
这次白女Abigail Fisher把AA告到最高法院，靠肤色种族搞AA已经难以维系了，录取学
生雇佣政府职员用肤色作为参考因素只是60年代的权益之策，现在法理上越来越无法自
圆其说。
可是diversity和老黑孩子成绩差还是liberal最关心的问题，NYT就提倡一个观点要考
虑收入，家庭财产，家庭结构，社区条件。
总之，世世代代最loser家庭的孩子，要最优先录取，父母祖辈越成功，孩子越排在后
面。大家争当loser，给下一代一个好的未来。
Rethinking Affirmative Action
-----
Racial gaps remain large enough that colleges would struggle to recruit as
many black... 阅读全帖 |
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M*****8
发帖数: 17722 | 9 【 以下文字转载自 Stock 讨论区 】
发信人: MB80528 (肥猫(Contrarian)[食MM而肥]), 信区: Stock
标 题: $$$ *** 疯狂大赠送最新(2013年4月24日晚)股票提款机101台 *** $$$
关键字: 股票；股市；股票预测；股票提款机；米国股市；
发信站: BBS 未名空间站 (Thu Apr 25 09:55:08 2013, 美东)
$$$ *** [置顶贴]疯狂大赠送最新(2013年4月24日晚)股票提款机101台 *** $$$
#001 AAMRQ: 看涨如果接近或低于 3.4738; 看跌如果接近或高于 4.7561
#002 AAPL: 看涨如果接近或低于 379.9088; 看跌如果接近或高于 411.0953
#003 ABIO: 看涨如果接近或低于 1.9197; 看跌如果接近或高于 2.8863
#004 ACAD: 看涨如果接近或低于 12.4369; 看跌如果接近或高于 14.7705
#005 ACI: 看涨如果接近或低于 4.3459; 看跌如果接近或高于 ... 阅读全帖 |
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h****n
发帖数: 2250 | 16 民主党担心力推affiative action 和英语西语双语教育，会把15%的亚裔推到共和党，
所以要开始分化亚裔，希望减少损失。
他们疯狗般地乱咬，说明他们目前还害怕亚裔的势力。越是疯狂，说明他们越是害怕。
同时，他们还想利用拿华裔作靶子，把西裔选民刺激出来，支持民主党。
几年以后，亚裔比例下去了，就不会有现在的恐惧了，更加肆无忌惮了。历史给我们的
机会，稍纵即逝，我们要珍惜。 |
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d**r
发帖数: 899 | 20 官方网站：http://asianamericancoalition.org/
EDITORIALS, OPINIONS & COMMENTARIES:
Wall Street Journal: Harvard’s Chinese Exclusion Act http://www.wsj.com/art
icles/harvards-chinese-exclusion-act-1433543969
Wall Street Journal: The New Jews of Harvard Admissions http://www.wsj.com/a
rticles/the-new-jews-of-harvard-admissions-1432077157
New York Post: Harvard discriminates against Asians as it once did to Jews h
ttp://nypost.com/2015/05/19/harvard-discriminates-against-asians-as-it-once-
did-to-jews
Na... 阅读全帖 |
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发帖数: 1 | 22 你看下币拍网能不能解决您的需要www.cnypai.com?-affi-3 |
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c*****g
发帖数: 3871 | 23 【 以下文字转载自 WaterWorld 讨论区 】
发信人: feebe (No TO SCA5), 信区: WaterWorld
标 题: 恩将仇报，仇将恩报的美国华裔
发信站: BBS 未名空间站 (Sun Feb 23 01:13:11 2014, 美东)
209法案是由共和党人士发起，导致亚裔入学比例上升
http://en.wikipedia.org/wiki/California_Proposition_209
Joe Gelman (President of the Board of Civil Service Commissioners of the
City of Los Angeles),
California Arnold Steinberg (a pollster and political strategist)
Dr. Larry Arnn President of the Claremont Institute
wiki上没有写他们的党派，我查了一下，全是共和党人士
http://wikibin.org/articles/joseph-gelman... 阅读全帖 |
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G*******9
发帖数: 4371 | 24 【 以下文字转载自 WaterWorld 讨论区 】
发信人: feebe (No TO SCA5), 信区: WaterWorld
标 题: 恩将仇报，仇将恩报的美国华裔
发信站: BBS 未名空间站 (Sun Feb 23 01:13:11 2014, 美东)
209法案是由共和党人士发起，导致亚裔入学比例上升
http://en.wikipedia.org/wiki/California_Proposition_209
Joe Gelman (President of the Board of Civil Service Commissioners of the
City of Los Angeles),
California Arnold Steinberg (a pollster and political strategist)
Dr. Larry Arnn President of the Claremont Institute
wiki上没有写他们的党派，我查了一下，全是共和党人士
http://wikibin.org/articles/joseph-gelman... 阅读全帖 |
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p***r
发帖数: 4859 | 25 Hackers Steal User Names and E-Mails from Zappos and 6PM.com
http://mashable.com/2012/01/16/hackers-zappos-6pm/
Hackers have broken into the data servers of online retailer Zappos and affi
liate site 6PM.com, stealing sensitive user data, the companies have announc
ed.
“We are writing to let you know that there may have been illegal and unauth
orized access to some of your customer account information on Zappos.com, in
cluding one or more of the following: your name, e-mail address, billing and
... 阅读全帖 |
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s******y
发帖数: 2361 | 26 Elizabeth Arden is offering free shipping + FREE 7-Piece Gift + Anti-aging
Serum with
any $49 order. via coupon code "SKIN49".
Offer ends 6/3.
http://shop.elizabetharden.com/family/index.jsp?
categoryId=3981470&utm_campaign=Skin49052611&cp=2779912&utm_source=CJ&utm_
medium=Affi
liate |
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g**********g
发帖数: 18118 | 27 VIP:
http://www.adorama.com/pages/vip?utm_term=Other&utm_medium=Affi
Adorama has Nikon D600 24.3MP Digital SLR Camera w/ 24-85mm f/3.5-4.5G ED AF
-S VR Lens & More + $50 Adorama Gift Card for $1996.95 when you checkout
with Visa card and enter coupon code VISA12B. Shipping is free. Thanks
yuugotserved |
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M*****8
发帖数: 17722 | 28 【 以下文字转载自 Stock 讨论区 】
发信人: MB80528 (肥猫(Contrarian)[食MM而肥]), 信区: Stock
标 题: $$$ *** 疯狂大赠送最新(2013年4月24日晚)股票提款机101台 *** $$$
关键字: 股票；股市；股票预测；股票提款机；米国股市；
发信站: BBS 未名空间站 (Thu Apr 25 09:55:08 2013, 美东)
$$$ *** [置顶贴]疯狂大赠送最新(2013年4月24日晚)股票提款机101台 *** $$$
#001 AAMRQ: 看涨如果接近或低于 3.4738; 看跌如果接近或高于 4.7561
#002 AAPL: 看涨如果接近或低于 379.9088; 看跌如果接近或高于 411.0953
#003 ABIO: 看涨如果接近或低于 1.9197; 看跌如果接近或高于 2.8863
#004 ACAD: 看涨如果接近或低于 12.4369; 看跌如果接近或高于 14.7705
#005 ACI: 看涨如果接近或低于 4.3459; 看跌如果接近或高于 ... 阅读全帖 |
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R*o
发帖数: 3781 | 30 G. Contribution of Passage to Systematic Theology
Romans 5:12-19 is a key passage in discussions about the origin of sin. It i
s clear from this text that as far as the human race is concerned, sin enter
ed through Adam. Further, there is a direct connection between the transgres
sion of Adam and the sin of the entire race. Any explanation that attempts t
o soften or do away with this direct connection falls on difficult times in
vv. 18-19 and throughout the passage. The best explanation, then, ... 阅读全帖 |
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c*****t
发帖数: 1879 | 31 Could anybody help me w/ drawImage function?
I have an IndexColorModel image and tried to draw it on screen.
When there is no resize, everything is okay.
However, when I try to draw the image with resizing, exception occurred.
The function that I used to draw image is
g.drawImage (m_image, 0, 0, m_width * 2, m_height * 2, null);
(* 2 is the scale factor);
The exception is
java.awt.image.ImagingOpException: Unable to transform src image
at java.awt.image.AffineTransformOp.filter(Affi |
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s***e
发帖数: 793 | 32 use two layer hash, it might look like this
my %a;
$a{"heilongjiang"}="haerbin";
my %b;
$b{"china"}=\%a;
print %{$b{"china"}}->{"heilongjiang"};
for reference in perl,
http://affy.blogspot.com/p5be/ch08.htm |
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x***u
发帖数: 297 | 33 mas5 已经是Normalized的了。再加其他的不太好。
MAS5 是比较老的Normalization方法，最初是给 3‘ Chip 做的，每个array是独立
normalized。
像affy这种 u133 plus 2 是probe level的 chp 用 RMA比较好。RMA会normalize
between chips，。 |
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x***u
发帖数: 297 | 34 mas5 已经是Normalized的了。再加其他的不太好。
MAS5 是比较老的Normalization方法，最初是给 3‘ Chip 做的，每个array是独立
normalized。
像affy这种 u133 plus 2 是probe level的 chp 用 RMA比较好。RMA会normalize
between chips，。 |
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a****a
发帖数: 3905 | 35 技术发展很难预计的。
不知道你这里microarray和DNA chip有什么区别，指PCR的print
和oligo Array之间的差别么？如果是这样的话
microarray较affy chip存在的理由，是PCR product的print并不需要
知道完全的序列，而chip制造的基础在于你需要知道sequence,构建
oligo库，制造芯片。
从数据的角度而言，两者的数据分析都存在着极大的variance, 所以实际
上，现在能利用的数据只是得到的数据的冰山一角而已。看过pat brown的
paper么？不做repeat, 做上上千种条件。对于每一张slide, 数据实际上
没有意义的，但是当把上千张slide cluster到一块的时候，这些数据就变
得有意义了。
从零一个角度而言，从现有的分析中，我们只能关注那些极端express或者
repress的基因，就如你列的那些文章，作为一般实验技术的前导与辅助，
去研究某个具体的基因，具体的条件而已，但这只是microarray/chip运用
的一个方面而已
如何能提高技术的精确度，如何能运用大量不是特别极端的数据，这是 |
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l*****k
发帖数: 587 | 36 you should read Affy manual, the P call percent depends on the sample
you used, and should be consistent for the same type of samples.
there are probe level methods other than MAS5 or GCOS, like dCHIP or RMA.
it is said they are better than MAS5, I suggest you to give them a try if you
have time. |
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h******b
发帖数: 312 | 37 There are primarily 2 types of microarray, comercial Affymetrix chip and
spotted array(cDNA/pcr), I guess u mean spotted array.
two major differences between the 2 types:
1. spotted material: affy use de novo synthesized oligo, 20mer or 25 mer,
called a probe, each gene is represented by 11? or 16 probes. the whole thing
called a probeset, so a probeset correspond to a gene. spotted array is
relative flexible, can print on the chip cDNA(comercially synthesized) or pcr
product, or even promoter s |
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s****6
发帖数: 34 | 38 Thank you very much for this wonderful sharing. While what you said is very
valuable and I appreciate it, I was asking about how to deal with those
undefined or uncharacterized genes, like those we met when we used the Affy
HG-U133_plus 2 chips. Some of the probes are from EST and unknow genes. I
got a few of them significantly changed in my treatment. Is it worthwhile to
pursue those "genes" for the sake of novelty? If yes, how to proceed? Do I
have a burden to prove these are truely some gene |
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t*d
发帖数: 1290 | 39 抱歉，误解了你的意思。还扬扬傻傻写了一大堆，呵呵。
这种 unknown 基因我一般是不去碰的。为什么呢？因为microarray结果的假阳性太高
了。就算你 rank 后，排在第一那个的都有可能是个假阳性。所以在做任何后续实验，
甚至简单的validation（用qPCR等方法）前，都最好综合各方面的知识，给candidate
genes排个序。然后决定试哪个。而这些未知的基因，后面要做的实验太多了。最难的
是找到它表达的蛋白，找到它的蛋白的抗体。够你折腾老长一段时间了。
当然，你有其它的证据。比如你发现在某个 GWAS 中，这个基因的 SNP 和某个疾病极
强相关，然后在你的与这个疾病相关的处理中表达变化了，然后qPCR验证了这个基因的
表达变化。这时也许值得一试。
very
Affy
to
I |
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H*****e
发帖数: 120 | 41 I have many samples for microarray. Try to save some money. Core charge me
for $600 for Affi-chip but only $400 for invitrogen MEEBO chip. In fact, I
was told that MEEBO is better even less people use it. Any one has any
suggestion?
Thanks! |
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f**r
发帖数: 70 | 42 fdr is an algorithm to calculate the adjusted p-value
controlling for the multiple comparisons (for example
if you used the Affy plus 2 chip you are making 54K
comparisons at once and even if there's no real signal
in your data whatsoever you are bound to get 54K*0.05
= 2700 significant genes if you simply use the p<0.05
cutoff). FDR controls the overall false discovery rate
for you so you should look at the fdr adjusted p-value,
use 0.05, or even 0.1 or 0.2 if you want to be liberal,
as your cu |
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n******7
发帖数: 12463 | 43 你做affy，应该多少会编程吧？
不过没有.edu的邮箱，总觉得不够诚意啊 |
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g******0
发帖数: 290 | 44 很久以前买的了，找不到manual了，网上也找不到，能请哪一位给我上传一份吗？多谢
多谢啦！ |
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g******0
发帖数: 290 | 46 多谢多谢啦，我也google了很久，就是没有找到，万分感激！ |
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o********r
发帖数: 775 | 47 I can’t agree with you at all on the performance validation. To be concise,
I followed the standard paradigm used in the community. You may only want to
challenge this, when (1) you prove concretely that this paradigm is wrong;
(2) you do not use this one in your own research. Your example on ‘RB1
related with RB’ is misleading and improper. One thing is sure here, a good
method has to be consistent, an inconsistent method is never a good method.
We can argue on this for days. But GAGE has been ... 阅读全帖 |
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d*******e
发帖数: 1649 | 49 you have to use exising software (e.g. R affy package) depending on your
platform.
？ |
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m***T
发帖数: 11058 | 50 APT - Affymetrix Power Tools，是Affy自己的软件工具，但不是commercial one。 |
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