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发帖数: 5204 | 2 Mounting the specimen
Selection of a mounting medium should
take into account the type of microscope objective
that will be used to observe the specimen
(see section on Microscope objectives).
In order for an objective to perform optimally,
the mounting medium should have the same
refractive index (RI) as the objective immersion
medium. Mismatches in the refractive indices
produce spherical aberration leading to
loss of light at the detector, as well as decreased
z-axis resolution and incorrect ... 阅读全帖 |
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