c*******n
发帖数: 1648 | 1 A Highly Selective and Sensitive Fluorescent Chemosensor for Hg2+ in
Neutral Buffer Aqueous Solution
Xiangfeng Guo,†,‡ Xuhong Qian,*,† and Lihua Jia†,§
State Key Laboratory of Fine Chemicals, Dalian UniVersity of Technology,
Dalian 116012, China,
Qiqihaer UniVersity, Qiqihaer 161006, China, and Shanghai Key Laboratory of
Chemical Biology, Institute of
Pesticides and Pharmarceuticals, East China UniVersity of Science and
Technology, Shanghai 200237, China
Received July 29, |
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T******n
发帖数: 77 | 2 http://www.xys.org/xys/ebooks/others/science/dajia13/zhujianguo
四川大学材料科学与工程学院朱建国和俞萍捏造实验数据一稿多投
方舟子,
我揭露一起赤裸裸的捏造实验数据一稿多投的科研造假卑劣行为，经查证，
论文的通讯作者为四川大学材料科学与工程学院院党委书记朱建国教授和俞萍副
教授。
最近按照文献[1] 2011年发表在Materials Characterization杂志上的实验
部分报道的方法合成Fe：ZnSe纳米粒子：先用Zn(CH3COO)2溶液在硫醇酸
(SHCH2COOH ) 的保护下与 NaHSe反应生成ZnSe,然后再将FeSO4和硫醇酸的混合
物在加热条件下与生成的ZnSe混合，再经分离洗涤和干燥后得到Fe掺杂的Fe：
ZnSe纳米晶体。做实验时，我对该报道的合成方法有些疑问，在合成的第一步中
ZnSe已经稳定的形成了，只经过第二步简单的二价Fe离子吸附和洗涤干燥，Fe怎
么就能那么容易的掺进ZnSe的晶格中呢？于是我又查找其它文献报道的方法，结
果惊奇的发现，献[1]中的实验数据（相同的图表和讨论）201... 阅读全帖 |
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c**y
发帖数: 377 | 3 Can you give some comments on protein structure and property difference in
aqueous phase and gas phase?
thanks~~ |
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z*h
发帖数: 773 | 4 What I talk about is to produce hydrogen from carbohydrate and water as
below reaction
C6H10O5 (aqueous) + 7 H2O (l) --> 12 H2 (g) + 6 CO2 (g).
Please check the H prize. No any chemical compounds can meet the requirement
for hydrogen storage. So use of hydrogen carriers will be better solution
NH3 --> 1/2 N2 + 3/2 H2
Methanol + H2O --> H2 + CO2
Among all possible carriers, carbohydrate will be the best due to costs,
efficiency, reaction conditions. |
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T**********t
发帖数: 1604 | 5 别的我不知道。
不过ethanol precipitation的recovery其实在室温下比在低温下更好。温度越低，
recovery越差。很多protocol建议低温，其实是出于degradation的考虑。有可能这个
trizol kit里加了很多酶抑制剂，所以不怕degradation，就可以室温操作了。
isopropanol的沉淀效率比ethanol高，所以需要的用量比ethanol少，适合用于浓度比
较低的核酸沉淀。但是isopropanol比ethanol难挥发，真空干燥用的时间更长，得到的
pellet也不如ethanol precipitation的pellet贴壁贴得牢。
至于你的chloroform wash不够effective的问题，也有可能是你吸取aqueous phase的
时候太贪心，想尽量把上层吸干净，结果反而夹带了一点phenol进来。我经常犯这种错
误，最后不得不再做一次chloroform wash，得不偿失。。。 |
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w******a
发帖数: 1527 | 6 Check this one:
Reagents
* TRIzol from Invitrogen #15596-108.
* Mini RNeasy plus kit from Qiagen #74134.
* Phase lock gel from Eppendorf #2302830.
* Keep TRIzol at 4C.
* Use RNase-free tubes and tips for all steps.
* Prepare 70% ethanol using DEPC-H2O and keep it at -20C.
RNeasy plus kit contains a genomic DNA elimination column. It can replace
the DNase I digestion step of regular RNeasy kit.
1. Add 1.2 ml TRIzol reagent to cells/tissues (5~10X 106 cells/ml or 50~100
mg ... 阅读全帖 |
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j*****9
发帖数: 716 | 7 http://scienceblogs.com/webeasties/2010/12/guest_post_arsenate-based_dna.php
In the wake of the NASA excitement over the new arsenic study, and my
promise to give a detailed review of the paper itself, I have recruited a
colleague with strong opinons about the work, a solid chemistry and
microbiology background, and "Dr." in front of his name to share his
analysis. I will be posting have posted my personal and less-technical take
on the whole thing soon, so stay tuned as well.
Dr. Alex Bradley u... 阅读全帖 |
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w***e
发帖数: 269 | 8 It is because the emission wavelength of a fluorophore is very much
influenced by the polarity of the micro-environment. A polar medium makes
the emission "red-shifted", meaning longer wavelength, whereas a non-polar
medium makes the emission blue shifted. Tryptophan is a hydrophobic amino
acid. In a folded protein, it is usually buried within the interior of the
protein (hydrophobic core) so that it is in a relatively non-polar
environment. In a completely unfolded protein, Tryptophan is expos... 阅读全帖 |
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x********u
发帖数: 430 | 9 Comments for Aji and Xiao's science paper
(1) the final product is taxadiene and taxadiene-5-O, there are still more
than 10 steps away from taxol
(2) the production reported is misleading. they used two phase fermentation,
taxadiene goes to the upper organic phase (1/10 of the volume of lower
aqueous phase). The real production should be divied by 10, whihc is around
100 mg/L |
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x********u
发帖数: 430 | 10 Comments for Aji and Xiao's science paper
(1) the final product is taxadiene and taxadiene-5-O, there are still more
than 10 steps away from taxol
(2) the production reported is misleading. they used two phase fermentation,
taxadiene goes to the upper organic phase (1/10 of the volume of lower
aqueous phase). The real production should be divied by 10, whihc is around
100 mg/L |
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c******6
发帖数: 928 | 11 there are two types of tubes, one is organic solvent-resistant, the other
one is not and can only be used for dialysis in aqueous environment. |
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l**********1
发帖数: 5204 | 12 庄只是徐福炼丹 给秦始皇炼那个长生不老丹吧?
Xiaoliang Sunney Xie
Raman Scattering is King Route to 单分子Raman scattering 光 (possible)
1)
Single DNA molecule detection in an optical trap using surface-enhanced Raman scattering
//apl.aip.org/resource/1/applab/v96/i21/p213701_s1
Abstract:
Raman spectra from single DNA molecules in their natural aqueous environment are presented. A DNA molecule that is anchored between two optically trapped dielectric beads is suspended in a solution with nanosized silver colloid particl... 阅读全帖 |
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l**********1
发帖数: 5204 | 13 文学水平如何 写散文或小说的出版记录的话 不是自费的或代笔( for example: Han-han) 的啊
然后找到以下的大牛 list中的任何一个, 千老混到发考题后的话
就忽悠纺锭啦 比如巴菲特的那个啥癌症的 如何用人工培养的天山千年冰雪莲 来药补
延寿 啥的
please refer:
同主题阅读：大家讨论一下synthetic biology吧
[版面:生物学][首篇作者：dengy] , 2011年03月30日
回复]
[ 13 ]
发信人: housepainter (红房子油漆匠), 信区: Biology
标 题: Re: 大家讨论一下synthetic biology吧
发信站: BBS 未名空间站 (Thu Mar 31 13:32:53 2011, 美东)
我老觉得还是已经故去的James Bailey才是真正的NB！后来从Caltech去了ETH，挺多8g
可以8的。虽然做的没那么fancy，还是挺牛的。他老去世的时候，BB也是出了专刊的，
鼓励他这一辈子在辛勤地在BB上灌了上百篇水。liao也写了篇回忆的文章。那一期BB上
还有个进化树，Bail... 阅读全帖 |
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b******y
发帖数: 627 | 14 DTT and BME are intrinsically unstable in aqueous solution. They will die
out within days. Use TCEP as it is more stable. After crystal trial is setup
, the system will be isolated in terms of O2. Further oxidation is prevented
. If you use high concentration of TECP, make sure to pH it right as it is
very acidic. |
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c*****t
发帖数: 198 | 15 【 以下文字转载自 Physics 讨论区 】
发信人: compact (support), 信区: Physics
标 题: 求一电子书的第四章
发信站: BBS 未名空间站 (Wed Aug 1 01:37:25 2012, 美东)
想读这一章。发现自己学校没有订阅这本电子书，但有的学校有。希望有的同学帮忙。
Molecular Theory of Water and Aqueous Solutions
Part II: The Role of Water in Protein Folding, Self-Assembly and Molecular
Recognition
多谢！ |
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l**********1
发帖数: 5204 | 16 Continue:
Microfluidic tools for high-throughput applications
Microfluidic strategies might hold the key towards fast, simple and
inexpensive high-throughput screening platforms These platforms could
revolutionize basic research in biology and medicine, in addition to
dramatically reduce costs in key industrial areas such as drug discovery. To
this end the development of microfluidic tools, capable of dealing with
miniaturized samples (and therefore with large amounts of information) are
needed.... 阅读全帖 |
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D*******r
发帖数: 162 | 17 papers are as below:
1.Stability of Vancomycin Hydrochloride Solutions at Various PH Values as
Determined by High-Performance Liquid Chromatography
Drug Development and Industrial Pharmacy ,1995, Vol. 21, No. 2 , Pages 257-
264 (doi:10.3109/03639049509048108)
2.Pharmaceutical Research
February 1987, Volume 4, Issue 1, pp 42-45
Stability of Lidocaine in Aqueous Solution: Effect of Temperature, pH,
Buffer, and Metal Ions on Amide Hydrolysis
please send to m*********[email protected]
Thanks! |
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E**********1
发帖数: 19 | 18 1. okay, first of all, I really don't know PDB actually provides predicted
3D models for every single PDB entry--maybe I am outdated. But if you could
provide everybody here with an example or a link, that would be very
helpful. I
am not sure if you are talking about homolog structures or homology
modeling. Neither is accurate information. Homology modeling is very
challenging especially when sequence homology is low. Often you cannot find
solved homolog structures to do that, am I right? ... 阅读全帖 |
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T******y
发帖数: 298 | 19 分别采用promaga公司的CellTiter 96® AQueous One Solution Cell
Proliferation Assay （MTS assay）和Invitrogen公司的PrestoBlue® Cell
Viability Reagent（Resazurin based PrestoBlue assay）检测一种抗癌药物对细胞
活性的影响。发现短时间（4h）药物刺激后，两种不同分析方法得到的结果竟是是相反
的。MTS分析显示细胞活性降低；而PrestoBlue分析显示细胞活性升高。大家说说怎么
回事？ |
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d******n
发帖数: 29 | 20 J Pharm Sci. 2013 Mar;102(3):813-26.
Investigation of histidine stabilizing effects on LDH during freeze-drying.
Al-Hussein A, Gieseler H.
Pharm Res. 2003 Dec;20(12):1952-60.
Influence of histidine on the stability and physical properties of a fully
human antibody in aqueous and solid forms.
Chen B, Bautista R, Yu K, Zapata GA, Mulkerrin MG, Chamow SM.
Protein Expr Purif. 2011 Nov;80(1):91-6. doi: 10.1016/j.pep.2011.05.014.
Epub 2011 May 26.
Effects of L-arginine on solubilization and purificati... 阅读全帖 |
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m********8
发帖数: 314 | 21 求助两篇文献。多谢!
Aspects of the degradation kinetics of doxorubicin in aqueous solution.
International Journal of Pharmaceutics. 1986, 32(2–3): 123–131
In Vivo Imaging of Bone Using a Deep-Red Fluorescent Molecular Probe Bearing
Multiple Iminodiacetate Groups. Mol. Pharmaceutics. 2013, 10 (11): 4263–
4271 |
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A******y
发帖数: 2041 | 22 Are you sure the one you buy is not already activated? EDC activation
require anhydrous condition (just go search online). Once Osu-ester is form
, it can react with amine in aqueous condition. If you think it is the
micelle, then you have to re-think you sequence of reaction.
COOH. |
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l****5
发帖数: 2968 | 23 请发g***********[email protected]
包子酬谢，多谢大家！
1. Effective natural dye extraction from different plant materials using
ultrasound
2. Anthocyanins: natural colorants with health-promoting properties.
Radish Anthocyanin Extract as a Natural Red Colorant for Maraschino Cherries
3. Separation and recovery of food coloring dyes using aqueous biphasic
extraction chromatographic resins
4. Recovery of anthocyanins from grape pomace extracts (Vitis vinifera L. cv
. Cabernet Mitos) using a polymeric adsorber resin... 阅读全帖 |
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c******r
发帖数: 59 | 24 Yes, they are dispersed in the aqueous solution to 0.5%. |
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S*****n
发帖数: 6055 | 25 Research topics:
microporous and mesoporous silica (for gas phase reaction) and carbon (for
aqueous phase reaction) supported catalysts
structure-relationship of bimetallic catalyst
Time-resolved spectroscopy for catalyst characterization
Research techniques:
Mostly used: adsorption (physical and chemical), TP technique, X-ray
absorption spectroscopies (XANES, EXAFS and NEXAFS), XRD, TEM, also
reactivity studies
Side techniques: IR, Raman, TG analysis, UV-vis-NIR, SEM, EPR |
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m***c
发帖数: 637 | 26 招聘：世界500强上海研发中心诚聘以下物理化学生物医药海外留学人士。
If you are interested, please email your CV to d****[email protected] with the
position that you are applying as the title.
Assistant Research Scientist-Food Science
Assistant Research Scientist-Polymer
Assistant Research Scientist-Polymer
Assistant Research Scientist-Rheology in Deposition
Assistant Research Scientist-Water Purification
Assistant Research Scientist –Deposition
Product Development Technologist-Skin GDC护肤类产品开发研究(For Skin
Category)
Research Associate (Me... 阅读全帖 |
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P*W
发帖数: 7 | 27 The Computational Materials Chemistry Lab at UC Riverside has an immediate
opening for a postdoc position in atomistic MD simulations of aqueous
inorganic-complex chemistry. If interested, please email djiang at ucr.edu
for details and also send your CV. |
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p**********m
发帖数: 472 | 29 Title: Bioanalytical liquid chromatography tandem mass spectrometry
methods on underivatized silica columns with aqueous/organic mobile phases
Source: Journal of chromatography A [0021-9673] Naidong yr:2003 vol:796
iss:2 pg:209 |
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k***l
发帖数: 25 | 30 2-Butanone (methyl ethyl ketone, MEK) [78-93-0] M 72.1, b 79.6o, d 4
20 0.853, n D
20
1.37850, n2 5 1.37612, pK2 5 -7.2 (aqueous H2SO4) . In general, purification
methods are the same as
for acetone. Aldehydes can be removed by refluxing with KMnO4 + CaO, until
the Schiff aldehyde test is
negative, prior to distillation. Shaking with saturated K2CO3, or passaging
through a small column of activated
alumina, removes cyclic impurities. The ketone can be dried by careful
distillation (an azeotrope |
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s********n
发帖数: 235 | 31 Title: PHOTOELECTROCATALYTIC REDUCTION OF CARBON-DIOXIDE IN AQUEOUS
SUSPENSIONS OF SEMICONDUCTOR POWDERS
Author(s): INOUE T, FUJISHIMA A, KONISHI S, et al.
Source: NATURE Volume: 277 Issue: 5698 Pages: 637-638 Published:
1979 |
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u******g
发帖数: 79 | 32 An Iodine/Triiodide Reduction Electrocatalyst for Aqueous and Organic Media
J. Electrochem. Soc., Volume 144, Issue 3, pp. 876-884 (1997)
http://dx.doi.org/10.1149/1.1837502
Thank you in advance. |
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w**********g
发帖数: 687 | 33 Removal of Hg2+ from aqueous solution using a novel composite carbon
adsorbent
Journal of Applied Polymer Science 112 (4), pp. 2445-2454 |
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d********O
发帖数: 1196 | 34 Title: Ion pairing in aqueous calcium chloride solution: Molecular dynamics
simulation and diffraction studies
Journal of Molecular Liquids
Volume 129, Issues 1-2, 15 October 2006, Pages 63-74
Please send to D********[email protected], if this is more convenient for you.
Thanks again and have a good day! |
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d********O
发帖数: 1196 | 35 Title:The speciation of aqueous zinc(II) bromide solutions to 500 °C
and 900 MPa determined using Raman spectroscopy
Year 2009 Volume 259, Issues 1-2, Pages 48-53
doi:10.1016/j.chemgeo.2008.08.014
Please send to D********[email protected], if it is more convenient to you.
Thanks a lot and Have a wonderful day! |
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r****1
发帖数: 2299 | 36 一个反应在PH 7左右，AQUEOUS SOLUTION里完成，但SAMPLE不溶于BUFFER，能不能加
DMF，会不会影响PH值，如果DMF占了至少50% BUFFER的作用完全没了吧。 |
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d********O
发帖数: 1196 | 38 Title: Equilibriums of ethylenediamine-N,N,N',N'-tetrakis(
methylenephosphonic) acid with copper(II), nickel(II), cobalt(II), zinc(II),
magnesium(II), calcium(II), and iron(III) ions in aqueous solution
Inorg. Chem., 1976, 15 (9), pp 2303–2306
DOI: 10.1021/ic50163a066
Please send to D********[email protected], if this is more convenient for you.
Thank you again and have a nice day. |
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T**y
发帖数: 673 | 39 DMF or DMSO: Very polar and high-boiling solvents that are difficult to
remove. If your product is not dangerously polar, dilute with lots and lots
of water before extracting with a nonpolar solvent. Then thoroughly wash the
organic layer with water.
For 5 mL of DMF or DMSO, use 5 X 10 mL of water during the aqueous wash.
This should remove all of the DMF or DMSO |
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l********g
发帖数: 30 | 40 希望得到稳定的磷酸钙 (hydroxyapatite) 的纳米颗粒水溶液(nanoparticle aqueous
suspension).
试了一些表面活性剂(SDBS, Pluronic acid, PEG),但是效果不是很理想，过24hr还是
出现团聚和沉淀.
溶液pH=6.7左右
Ionic strength= 0.1 M NaCl
thanks a lot! |
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a*****c
发帖数: 3525 | 41 http://www.informaworld.com/smpp/content~content=a908791066~db=all
Organic Preparations and Procedures International, Volume 21, Issue 2 April
1989 , pages 135 - 139
THE PREPARATION OF 1-(agr-AMINOALKYL) - BENZOTRIAZOLES IN AQUEOUS SOLUTION
Authors: Alan R. Katritzky a; Boguslaw Pilarski a;Laszlo Urogdi a
please send to my email: a*****[email protected]
also, please leave your ID, I will reward you a baozi.
Thanks a lot. |
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P***e
发帖数: 804 | 42 PTFE is not water-permeable, therefore not suitable for aqueous samples
where cellulose or nylon filters can be used. |
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x********e
发帖数: 1064 | 45 Polyisobutylene-block-poly(methacrylic acid) Diblock Copolymers: Self-
Assembly in Aqueous Media
Langmuir, 2007, 23 (26), pp 12864–12874
Please sent to t*********[email protected]
Thanks |
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d********O
发帖数: 1196 | 46 Title: Complexation Equilibrium Constants of Poly(vinyl alcohol)-Borax
Dilute Aqueous Solutions – Consideration of Electrostatic Charge Repulsion
and Free Ions Charge Shielding Effect
Volume 9, Number 4 / December, 2002
DOI 10.1023/A:1021351701568
Please send to D********[email protected] , if this is more convenient for you.
Thanks again! |
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y***k
发帖数: 57 | 47 It is interesting, but it is hard to be applied in industrial. Given the low
voltage of this aqueous Li ion battery system, it will have low energy and
power density. It seems against the trend of current Li ion battery R&D for
higher energy density. |
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