n***w
发帖数: 2405 | 1 I don't know.
Maybe some neurons like pyramidal cells.
If it's astrocyte, it should not limited to cortex. |
|
c*********3
发帖数: 129 | 2 首先谢谢大家的指导。这是DAB染的色。也另外染了GFAP，和她不一样。不像完全是
astrocyte. |
|
e**f
发帖数: 144 | 3 要做GFAP,O4,Tuj1 triple labeling
GFAP 是rabbit的，O4,Tuj1都是mouse的，请问有没有好的非 rabbit,非mouse的O4或者
Tuj1 抗体可以推荐？
谢谢！
另外，astrocytes-specific marker,除了GFAP，还有别的吗？ |
|
t*d
发帖数: 1290 | 4 最近接到一个项目，需要找 neuron secreted proteins。有没有这方面的 proteomics
的报导？找到一堆 astrocyte 的，就找不到 neuron 的。
谢谢了先！ |
|
n***w
发帖数: 2405 | 5 Astrocytic-neuronal crosstalk: Implications for neuroprotection from brain
injury.
PMID: 21693140
Please send to n***[email protected]
Thank you! |
|
e*******r
发帖数: 275 | 6 thanks so much
towards
that
exhibit |
|
|
j****1
发帖数: 81 | 8 Dedifferentiation of Neurons and Astrocytes by Oncogenes Can Induce Gliomas
in Mice
Science DOI: 10.1126/science.1226929
邮箱：j********[email protected]
谢谢！：） |
|
c********n
发帖数: 67 | 9 1, Ultrastructure of rabbit retinal nerve fibre layer--neuro-glial
relationships, myelination, and nerve fibre spectrum
2, Circadian rhythmicity in neutrophil chemotaxis.
3, Concentration of astrocytic filaments at the retinal optic nerve junction
is coincident with the absence of intra-retinal myelination: comparative
and developmental evidence
h***[email protected]
Thanks very much for you kindly help |
|
j*******g
发帖数: 10 | 10 thanks a lot!
Current Neurovascular Research
ISSN (Print): 1567-2026
ISSN (Online): 1875-5739
VOLUME: 9
ISSUE: 1
DOI: 10.2174/156720212799297100
PDGF Receptor β Signaling in Pericytes Following Ischemic Brain Injury
Author(s): Koichi Arimura, Tetsuro Ago, Masahiro Kamouchi, Kuniyuki Nakamura
, Koji Ishitsuka, Junya Kuroda, Hiroshi Sugimori, Hiroaki Ooboshi, Tomio
Sasaki and Takanari Kitazono
Pages 1-9 (9)
Abstract:
Platelet derived growth factor (PDGF)-B plays a neuroprotective role in
brain dam... 阅读全帖 |
|
n*******l
发帖数: 190 | 11 MACS已经很简单了，但是glial progenitor cells也表达glast |
|
p*****c
发帖数: 20445 | 12 Marc R. Freeman, Ph.D.
HHMI Early Career Scientist
University of Massachusetts Medical School
Neurons hog the attention, but our brains couldn’t operate without the
unassuming neural cells called glia. Marc Freeman is working to bring these
overlooked cells—which represent about 60 percent of brain cells—their
share of credit.
Freeman has been dissecting the role of glia in brain development, function,
and healing after injury, when glia hurry to the damaged area and devour
cellular debris to al... 阅读全帖 |
|
n***w
发帖数: 2405 | 13 what is your time course in this exp?
I think it is normal for a first upregulation of MAP2 and then decrease
during astrocyte development.
I am not an expert, just guessing. |
|
k******0
发帖数: 1073 | 14 6月5日出版的最新一期《Nature Medicine》(Nature Medicine 19, 773–777，2013)
发表了南方医科大学高天明、朱心红教授团队题为“星形胶质细胞来源的三磷酸腺调节
抑郁样行为（Astrocyte-derived ATP modulates depressive-like behaviors）”的
研究论文。该研究揭示了抑郁症发生新机制--星形胶质细胞ATP释放异常，并为抗抑郁
药的研究提供了新思路。
抑郁症是最为常见的心境障碍，以其高发病率、高复发率并伴有严重自杀倾向，已
成为严重危害人类生命与健康的重大公共卫生问题。但目前尚不清楚抑郁症的发病机制
，临床上也缺乏起效快的抗抑郁药。
以往国际上主要针对单胺类神经递质系统研发抗抑郁药，但药物的起效慢（常需3-
4周），许多患者往往很难坚持到取得疗效便放弃治疗，因此研发起效快的抗抑郁药已
成为一个十分迫切需要解决的问题。脑内主要有两种细胞即神经元和胶质细胞。长期以
来，人们认为胶质细胞在脑功能执行中仅是一个配角，但近年越来越多的证据显示胶质
细胞在调控脑功能中具有举足轻重的作用。临床尸检及动物药理学实... 阅读全帖 |
|
m**e
发帖数: 857 | 15 How can iPSC self-organize into a functional liver?
If it is possible, can we culture Neuron-oriented iPSC with astrocyte and
get a functional Brain? |
|
|
|
h******y
发帖数: 351 | 18 Hygromycin浓度偏高了。你有没有先做一下kill curve找出合适的antibiotic浓度？
合适的hygromycin通常在10-30 ug/mL
合适的puromycin通常在0.3-1 ug/mL |
|
|
t****t
发帖数: 86 | 20 5小鼠神经干细胞，想分化成neuron, 用beta III tubulin 染色， 发现比例非常低，
大多数细胞都是GFAP+ astrocytes。 而且第四，五天开始，细胞开始死亡，有凋亡小
泡。分化用的medium是前人留下的配方，neurobasal A + B27 + 1% HI-FBS + 2mM
Glutamax + 0.5uM RA。每天换一半液。
折腾两个月了。。。求指教 |
|
a****d
发帖数: 1919 | 21 我手上的primary NSC分化的时候会有一定量的cell death，可以和astrocyte
coculture来促进survival. |
|
t****t
发帖数: 86 | 22 我最后要算百分比，所以可能不方便加astrocyte
我今天又看了看，加了RA的孔细胞死的非常多，没加的基本只有很少量的死亡。看来是
RA的问题。我会试试GDNF和BDNF
我听说神经干细胞分化不处理的baseline都有15-20%会变成neuron,这个是指的primary
还是cell line? 我怎么感觉我这个primary NSC没这么高啊 |
|
d**l
发帖数: 1546 | 23 请问哪里有转基因的神经方面的细胞库？我要找的是一个转基因老鼠的。 谢谢 |
|
|
S******9
发帖数: 2837 | 25 这个难道不是你选定一个研究的target基因的flox老鼠和Cre老鼠杂交以后就得到的？ |
|
|
|
h*******o
发帖数: 4884 | 28 别想了
可能性极小
找有那个老鼠colony的实验室合作算了 |
|
d****h
发帖数: 46 | 29 For rat neuron
GABRA1: (Neuron. 1989 Dec;3(6):745-53.A novel alpha subunit in rat brain
GABAA receptors.Khrestchatisky M, MacLennan AJ, Chiang MY, Xu WT, Jackson MB
, Brecha N, Sternini C, Olsen RW, Tobin AJ.)
GABBR2:similar to GABRA1, highly neuron-specific
MAP2:(A Harada, J Teng, Y Takei, K Oguchi, N Hirokawa
MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and
proper PKA signal transduction J Cell Biol, 158 (2002), pp. 541–549)
Tuj1: (J Neurobiol. 1995 May;27(1):26-43.Div... 阅读全帖 |
|
O**********5
发帖数: 3 | 30 Two positions for Postdoctoral Research Scientists
We are seeking highly qualified and motivated candidates who share a passion
for scientific discovery to join an interactive team.
Post #1: The molecular and cellular mechanisms controlling the balance of
immune response in the mouse brain.
Chronic neuroinflammation is a common feature of the ageing brain and a
variety of neurodegenerative disorders. However, the molecular and cellular
mechanisms underlying the regulation of innate immunity in t... 阅读全帖 |
|
发帖数: 1 | 31 最近查询文章，发现跟帕金森病相关的LRRK2研究一个有趣现象，其中有一篇是现任北
理工的庆宏教授发表的LRRK2能够直接结合a-Synuclein并且磷酸化Serine-129位点。
但是后续大量的综述讨论的时候提到他这篇文章时说this is the only one paper
show LRRK2 can phosphorylate S129-synuclein。我看他发表的这篇BBRC已经被引用
快超100次了，如果文章的结论有问题，那岂不是严重阻碍了关于LRRK2-G2019S的研究
？大伙有兴趣的可以来共同探讨，或者找庆教授讨论。电子邮件：[email protected]/* */
个人简历
教育背景：
1993.9-1996.7：博士，广州中山医科大学，神经生物学
工作经历：
2006.2- 至今：北京理工大学生命科学与技术学院，教授
2001.9 – 2006.2：英属哥伦比亚大学精神病学系，脑研究中心，研究员
2000.7 – 2001.8：加拿大萨斯喀彻温大学，神经精神病学系，博士后
1997.7- 2000.6：美国埃默里大学医学院，放射肿瘤系与细胞... 阅读全帖 |
|
b********h
发帖数: 2481 | 32 Astrocytomas are tumors that arise from astrocytes. They were graded on a
scale from I to IV. Astrocytoma grade IV is glioblastoma. |
|
发帖数: 1 | 33 您好，我的朋友是生物统计方向的master，她有很多publication和审稿的经验。她在
德州西南医学中心做computation biologist。以下是她的信息
name: Uthra Balaji
email: [email protected]/* */
如果有合适的审稿机会可以发给她吗？多谢您！！
Publication:
1. Metabolic Reprogramming of Pancreatic Cancer Mediated by CDK4/6
Inhibition Elicits Unique Vulnerabilities. Jorge Franco, Uthra Balaji,
Elizabeth Freinkman, Agnieszka K. Witkiewicz, Erik S. Knudsen, Cell Reports
2016.
2. Whole-exome sequencing of pancreatic cancer defines genetic diversity and
therapeutic targets. Agnie... 阅读全帖 |
|
发帖数: 1 | 34 您好，我的朋友是生物统计方向的master，她有很多publication和审稿的经验。她在
德州西南医学中心做computation biologist。以下是她的信息
name: Uthra Balaji
email: [email protected]/* */
如果有合适的审稿机会可以发给她吗？多谢您！！
Publication:
1. Metabolic Reprogramming of Pancreatic Cancer Mediated by CDK4/6
Inhibition Elicits Unique Vulnerabilities. Jorge Franco, Uthra Balaji,
Elizabeth Freinkman, Agnieszka K. Witkiewicz, Erik S. Knudsen, Cell Reports
2016.
2. Whole-exome sequencing of pancreatic cancer defines genetic diversity and
therapeutic targets. Agnie... 阅读全帖 |
|
f**d
发帖数: 768 | 35 http://online.itp.ucsb.edu/online/neuro10/
有兴趣的同学可以观看其中视频cam链接，效果很清晰，
学习的同时也可以非常好地练习英语听力
KITP Program: Emerging Techniques in Neuroscience (Sep 20 - Nov 12,
2010) Coordinators: Adrienne Fairhall, David Kleinfeld, Fred Wolf
Speakers: Please see us about file upload for your slides.
会议内容涉及实验和计算神经科学的比较广泛的课题
Time
Speaker
Title
9/20, 10:15 a.m.
All
Greeting & Salutations from Adrienne, David & Fred[Podcast][Aud][Cam]
9/20, 10:30 a.m.
John Hopfield
Princeton, IAS
How Have Experiment and Theory Informe... 阅读全帖 |
|
O**********5
发帖数: 3 | 36 Two positions for Postdoctoral Research Scientists
We are seeking highly qualified and motivated candidates who share a passion
for scientific discovery to join an interactive team.
Post #1: The molecular and cellular mechanisms controlling the balance of
immune response in the mouse brain.
Chronic neuroinflammation is a common feature of the ageing brain and a
variety of neurodegenerative disorders. However, the molecular and cellular
mechanisms underlying the regulation of innate immunity in t... 阅读全帖 |
|
