xf 发帖数: 68 | 1 我有一个朋友,今年暑假要毕业。是IVY动物系的,研究家禽的。
有几篇Poultry Science的文章,
大家看看这样的研究经历和会的技术手段找什么样的博士后又机会。工业界如何呢?
他老板快退休了,也不知道能有多大帮忙。
谢谢
RESEARCH EXPERIENCE
• Designed and performed experiments to investigate the embryonic
development of chicken under various cholesterol-depleted conditions.
• Modified and improved the yolk replacement technique.
• Determined the cholesterol requirement for embryos to develop,
hatch, and grow normally.
• Determined the toxic level of beta-cyclodext... 阅读全帖 |
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n********k 发帖数: 2818 | 2 google is ur best friend...anyway, in general, it is denature reagent and/or
detergent including DMSO, Betaine, NP40, Triton-X100 etc etc...what I heard
Betaine is likely the best for most of application...and u can buy it from
some company...nonetheless, it could still be tricky even if you know the
gradient, it could still take a hell a lot effort to figure out correct
composition...I personally have used mostly DMSO, and Q solution from qiagen
and a bit betaine... |
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f******r 发帖数: 924 | 3 妈妈从国内来,带的是苯磺酸氨氯地平分散片,吃了一个月就吃完了。想要我帮她买一
些抗血压或是预防高血压的保健品。妈妈不挑食,不吸烟,不喝酒,160cm,130斤。
在网上查了一些,有一个叫Natural Way Beet Root 据说是欧洲人发现的一种植物的有
预防高血压的功效。
Description:(Nature's Way Beet Root Powder is encapsulated particularly
for those who find the taste of beets unpleasant. Beet powder provides a
wide range of nutrients, but its most significant phytochemical is betaine.
This plant chemical helps the liver and kidneys recycle the amino acid
methionine to maintain the body's stores of s-adenosyl-methionine, ... 阅读全帖 |
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s*******y 发帖数: 384 | 4 drugstore有~~~的确是不含sls,不过我看见drugstore线上的那些avalon产品
多半是已经添加有精油的,或者是有植物萃取成分,多半还是有些味道,
再添加别的精油浓度不太好掌握,成份我copy了一份
里面有熏衣草花水,芦荟,依兰精油,柠檬和莱姆精油。
洗净成分是Olefin Sulfonate, Cocamidopropyl Betaine,和 Citric Acid,
产品名称:
Avalon Organic Botanicals Therapeutic Shampoo, Nourishing Lavender
成份如下
Certified Organic Lavender Floral Waters, Herbal Infusion of Certified Organic
Ingredients (See product details for ingredients), Yucca, Organic Sea Kelp,
Olefin Sulfonate, Cocamidopropyl Betaine, Certified Organic Aloe Vera, |
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g**********r 发帖数: 422 | 5 再求另一个:
http://pubs.acs.org/doi/abs/10.1021/bk-2012-1120.ch028
题目
Poly(ethylene glycol) and Poly(carboxy betaine) Based Nonfouling
Architectures: Review and Current Efforts
作者:
Mojtaba Binazadeh, Maryam Kabiri, Larry D. Unsworth
来源:
Proteins at Interfaces III State of the Art
Chapter 28, pp 621–643
ACS Symposium Series, Vol. 1120
请发到k******[email protected]
谢谢 |
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S*****o 发帖数: 131 | 6 看你怎么消化吸收了 - below is from wikipedia
Spinach has a high nutritional value and is extremely rich in antioxidants,
especially when fresh, steamed, or quickly boiled. It is a rich source of
vitamin A (and especially high in lutein), vitamin C, vitamin E, vitamin K,
magnesium, manganese, folate, betaine, iron, vitamin B2, calcium, potassium,
vitamin B6, folic acid, copper, protein, phosphorus, zinc, niacin, selenium
and omega-3 fatty acids. Recently, opioid peptides called rubiscolins have
also been ... 阅读全帖 |
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w***s 发帖数: 7132 | 7 英文/中文 护肤品成分对照表2006-07-28
Tag:护肤品常识
版权声明:转载时请以超链接形式标明文章原始出处和作者信息及本声明
http://drer5.blogbus.com/logs/2927288.html
你必须知道的常见护肤品成分:
水:water/aqua,purified(纯净水)
酒精:alcohol,SD Alcohol 40,Alcohol denat
香料:fragrance/parfum
色素:种类很多,一般从是否出现颜色的单词来判断,比如CL42090、blue1,Red4
保湿剂:常见的是Glycerin,Sodium```,还有很多其他种类
Hydratant/Hydra/Moisturize=保湿
Pore Minimising/Pore Reducer=收缩,细致毛孔
Lift/Firming/Contouring=紧实,提拉
Sebum/Shine/Beilliance=控制皮质分泌
Nutri/Nutritive/Nourishing=丰富营养,滋润
Repair/Anti-Age=修复,抗衰老
Exfoliant/Gommage/Scru... 阅读全帖 |
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d**********u 发帖数: 727 | 8
我在做fasting hiit, pre10g post 10g. 还混着酸不拉唧臭烘烘的Betaine,所以感受
特别深 |
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n***e 发帖数: 180 | 9 Phusion的话GC buffer有3.5%DMSO,其它的酶有可能是betaine |
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k****l 发帖数: 279 | 10 For 1.3kb, it's cheap to synthesize. But it shouldn't be very difficult to
amplify. 24bp primer is too long, 18 is enough.
You can try epicentre fail-safe PCR kit. There are 12 buffers with different
Mg2+ and betaine combinations. Usually some of them should work. |
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s********n 发帖数: 2939 | 11 0.5-2 M betaine and 1-10% DMSO. |
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s****l 发帖数: 395 | 12 可以在PCR反应体系中加点Betaine(final concentration好像是1M)看看 |
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d****u 发帖数: 1553 | 13 betaine是个办法,还可以加DMSO,还有一些公司提供GC RICH reagent。比如罗氏。 |
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w********r 发帖数: 1431 | 14 对高GC的PCR,加1M betaine,dCTP/dGTP的量加倍,
然后DMSO 3-7% 和 退火温度一起,做一个二维的梯度试一下条件。
这个条件对付GC rich PCR,用了的都说好。
good luck!
ATG |
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z*t 发帖数: 863 | 15 目前在用real-time PCR扩一段基因组上GC rich region,设计了几条引物扩增不同的区
域,普通PCR跑胶没有见到可见的smear或者dimer。用ABI的SYBR green master mix
dissociation curve能见到多个peak,amplication curve也很奇怪。跑胶可以看到
smear和dimer...最简单的方法是换引物。。。但是俺要扩的区域CG含量很高。。。换
了primer估计还会有类似问题。。。
对付high CG常用的DMSO/Betaine啥的,在real-time PCR中有没有类似应用?
求做过high gc real-time PCR的大侠指点迷津啊 |
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s******y 发帖数: 28562 | 16 可能是二级结构的问题。
不妨加点DMSO or betaine 试试 |
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s********r 发帖数: 312 | 17 我搞过一次80个bp的,必须25ul体系,不能配mastermix,然后加betaine |
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g***y 发帖数: 201 | 18 1. use betain instead of DMSO. I tried them side by side and found that
when I used DMSO, there was increased error rate.
2. try phusion:taq 1:1 mixture instead of phusion alone. |
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s*****i 发帖数: 315 | 19 + 10% 5M betain 试试,sigma有卖
★ 发自iPhone App: ChineseWeb 8.7 |
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y*****0 发帖数: 15 | 20 已经试过DMSO了,没有什么帮助。也听说betain对GC rich的gDNA PCR有帮助,正准备
试试。其实这个PCR还真不是一般的PCR,是用CRISPR/Cas library lentivirus 感染
后,用PCR扩增integrated gRNA,难度很大啊。谁做过这个screen,可以交流一下。万
分谢谢! |
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s*********9 发帖数: 48 | 21 请教一下前辈,
常用ISI,google,关键词少,搜到文献太多太杂,关键词多,又搜不到文献
sci finder 太慢,不适合搜物理参数。
不知道哪位能提供个更好的搜索引擎,或是指点如何用好ISI?
具体问题:
1. search for Refractive index of metamaterials;
2. search for T1 of different C-13 peaks of Cocamidopropyl betaine.
另外,改版后的ISI中,找不到citation times, 能不能告诉在哪有? |
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p****u 发帖数: 1940 | 22 老板想要下面这些东西溶液的viscosity,不知道我应该在什么地方查找?
Norleucine
Phenylalanine
1-octanol
Na+ Octanoate
Glycerol
Xylose
DHA
Glucose
Sucrose
Sorbital
Trimethylamine
Betaine
Proline
Sarcosine
那个大虾能给点方向阿? 跪谢!!! |
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w********g 发帖数: 447 | 23 betaine
major fragment is 58 and 59. |
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z**n 发帖数: 22303 | 26 谢谢.
药名: 地骨皮
别名: 杞根、地骨、地辅、地节、枸杞根、苟起根、枸杞根皮、山杞子根、甜齿牙根、红耳堕根、山枸杞根、狗奶子根皮、红榴根皮、狗地芽皮
汉语拼音: di gu pi
拉丁文名: Cortex Lycii Chinensis
英文名: Chinese Wolfberry, Chinese Wolfberry Root-bark, Root-bark of Chinese Wolfberry, Root-bark of Barbary Wolfberry
拉丁植物动物矿物名: Lycium chinense Mill.
性味: 味甘;性寒
归经: 归肺;肾经
功效: 清虚热;泻肺火;凉血
主治: 阴虚劳热;骨蒸盗汗;小儿疳积发热;肺热喘咳;吐血;衄血;尿血;消渴
用法用量: 内服:煎汤,9-15g;大剂量可用15-30g。
用药禁忌: 1.《医学入门》:忌铁。
2.《本草汇言》:虚劳火旺而脾胃薄弱,食少泄泻者宜减之。
3.《本草正》:假热者勿用。
功效分类: 清热药;凉血止血药
各家论述: 1.李杲:四物汤内加地骨皮、牡丹皮,治妇人骨蒸最妙。地骨皮治足少阴、手少阳有汗而骨... 阅读全帖 |
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s*********9 发帖数: 48 | 27 请教一下前辈,
常用ISI,google,关键词少,搜到文献太多太杂,关键词多,又搜不到文献
sci finder 太慢,不适合搜物理参数。
不知道哪位能提供个更好的搜索引擎,或是指点如何用好ISI?
具体问题:
1. search for Refractive index of metamaterials;
2. search for T1 of different C-13 peaks of Cocamidopropyl betaine.
另外,改版后的ISI中,找不到citation times, 能不能告诉在哪有? |
|