C***k
发帖数: 1570 | 1 帮师弟问的，纯生物背景，选Ph.D thesis实验室，生物谈不上非常喜欢，比较care以
后的工作前景，现在有机会进一个做mass spectrometry的实验室做Ph.D student，主
要是分析biomarker之类的project，老板比较有名，学生找工作传说都不错，只是因为
没有化学基础，而且实验室太大，老板经常不在，担心不能得到很好的training，另外
还有一个实验室做cell biology，老板很nice，对学生很好，实验室气氛也不错，不过
publication一般（多为JBC,MCB）。
我个人不懂mass spec,这里想听听大家的建议选哪一个好，生物背景的做mass会不会很
吃力，以后在工业界和学术界的发展如何？因为传说做mass去工业界找工作很容易，所
以特来请教，先谢了。 |
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M**a
发帖数: 4816 | 2 Postdoctoral and medical fellow positions are available to participate in
ongoing studies of molecular and cellular neurobiology in developmental and
aged related brain disorders as well as clinical biomarker searching/
validation in Alzheimer’s disease. Candidates with a strong background in
molecular biology, including molecular cloning, vector design and
construction, enzyme assays and purification or cell biology, including
primary cell cultures of neurons, glia, endothelial cells and stem c |
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s****0
发帖数: 2532 | 3 Biomarkers in spinal cord injury.
Spinal Cord 47, 519-525 (July 2009).
谢谢！ |
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S******G
发帖数: 965 | 4 stress level --> immunology responses --> moniter all cytokines levels -->
understand positive correlations between stress level with certain
biomarkers with clinical indications |
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m******i
发帖数: 73 | 5 【 以下文字转载自 Chemistry 讨论区 】
发信人: mutouhui (mutouhui), 信区: Chemistry
标 题: Postdoc postions in LC-mass spectrometry quantitation
发信站: BBS 未名空间站 (Tue Jun 15 11:59:36 2010, 美东)
Two Postdoc positions are available immediately at University of North
Carolina at Chapel Hill. We are working on quantitation of carcinogen-
induced DNA adducts and protein biomarkers. The lab is equipped with 3
Triple quadrupole mass spectrometers, 1 ion trap, 1 ICP-MS and 1 Q-TOF. We
also have several HPLC, UPLC and nano-LC |
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w********e
发帖数: 110 | 6 A postdoctoral position is available immediately in the Signal Transduction
Laboratory, Department of Gynecology and Obstetrics at St. Joseph’s
Hospital and Medical Center located in Phoenix, Arizona. Our laboratory is
focused on the study of signal transduction pathways and implications in
endocrine-related cancers, diabetes, and obesity, using cell culture system,
animal models as well as specimens from human subjects. Our long-term goal
is to discover potential therapeutic biomarkers and ne |
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R**********n
发帖数: 473 | 7 我朋友正为上海联合利华招聘美国博士（后）毕业生人才。
The following is the job description.
Description of main responsibilities
•Build up the toolbox mainly in vitro to identify actives with
cardiovascular benefits for functional foods. •Build mechanistic
understanding on biomarkers and pathways relevant to cardiovascular diseases
and metabolic syndrome.
•Build network with the key players in the area.Professional Skills:
•Ph.D. or postdoc. in cardiovascular pharmacology or physiology is
essential |
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S***s
发帖数: 67 | 8
If interested, please contact Dr. Happy Li at h******[email protected]. Thanks.
=========================================================
Working location: Unilever R&D Shanghai
Job title: Assistant scientist or scientist
Contactperson: Dr. Happy Li (h******[email protected])
Description of main responsibilities
* Build up the toolbox mainly in vitro to identify actives with
cardiovascular benefits for functional foods.
* Build mechanistic understanding on biomarke |
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w******e
发帖数: 1187 | 9 同意你说的问题。特异性方面，主要是biomarker的validation严重滞后于
discovery。查文献可以找出一堆特异性marker，鬼都不follow up。。。
AACR上听了一个early marker validation的initiative，很多negative data。。。
deliver方面，问题的根源在于大家都柿子捡软的捏，能只做cell culture
绝不做老鼠，能只做intratumor injection决不做systemic administration，
能只做subcutaeous model by tumor cell injection决不做orthotopic/GEM model，
一旦热点过了就换一个概念接着从最低级的model做起（话说到了2006用aptamer
还能只做cell culture就发nature biotech，到了2009还能用aptamer+NP只做
cell culture发angwandte chemie。。。可惜等我跳进来好日子就到头了。。。），
当然对real worldproblem指导意义有限。。。 |
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t*d
发帖数: 1290 | 10 没有研究过 PKPD。不过说道这个，俺偏一点题：如果日后精确制导的药物传输可以实
现的话（比如通过 nano particle，改造的virus），做个新药是不是就简单多了？
比起用 zebrafish，我个人觉得找 toxicity biomarkers 可能更靠谱一点（纯个人不
靠谱感觉，呵呵）。
就在
molecul
fish
pfizer和
结果
全不 |
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p*****m
发帖数: 7030 | 11 不一定吧 PKPD不光有delivery的问题 我觉得更主要的还有药物进了细胞怎么被修饰 被
降解等等blabla的问题 这些还是要到animal上看才行的
能不能展开说说toxicity biomarker? |
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R****n
发帖数: 708 | 12 斑竹能把这个展开说说不？现在要进这样的一个实验室，已经面试了，感觉还不错。但
是我以前是做biomarker的，他们需要这方面的人。
，除非你研究的是非常接近临床应用的（比方说研究不同的放射剂量 |
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w******y
发帖数: 8040 | 13 hehe, case by case了
成功的药当然不希望有marker
但得先保证成功， 这就是biomarker的用武之地了
disability
project |
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K******S
发帖数: 10109 | 14 pubmed search "proteomics, cancer biomarker", 10s of thousands of them per
year. FDA approves less than 1 per year. go figure.
hehe |
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K******S
发帖数: 10109 | 15 Actually, that's not my original intention.
NCI still pours tons of money into the field.
The problem is nobody can get fund for validation projects and the bar set
for "biomarker" is way too low.
rare
it. |
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l****y
发帖数: 398 | 16 I have this silly question in my mind: can they reliably tell if the sample
is from a male or female by using any
proteomic "biomarker" from 体液? |
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O******e
发帖数: 4845 | 17 http://www.nature.com/news/2010/100922/full/467380a.html
China pushes for the proteome
Strategy to build a complete catalogue of human proteins could put the c
ountry in a leading position.
David Cyranoski
Proteomics could turn up biomarkers to help diagnose disease faster than
current tests.Xinhua/LANDOV/PA
First the genome; now the proteome. China has already established a lead
ing position in DNA sequencing through the work of the BGI in Shenzhen (
formerly the Beijing Genomics Institute), wh |
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s**u
发帖数: 9035 | 18 1.Technol Cancer Res Treat. 2010 Apr;9(2):123-38.
Non-coding RNAs: identification of cancer-associated microRNAs by gene
profiling.
Ferdin J, Kunej T, Calin GA.
Department of Animal Science Biotechnical Faculty, University of Ljubljana,
Domzale, Slovenia. g****[email protected]
2.Expert Rev Mol Diagn. 2010 May;10(4):435-44.
miRNAs as molecular biomarkers of cancer.
Fabbri M.
Department of Molecular Virology, Immunology and Medical Genetics. The Ohio
State University Comprehensive Cancer Center, C... 阅读全帖 |
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a*****y
发帖数: 269 | 20 【 以下文字转载自 SanDiego 讨论区 】
发信人: alvinpy (生命就是一场歪打正着), 信区: SanDiego
标 题: Post-Doc Positions at Pfizer
发信站: BBS 未名空间站 (Tue Nov 16 20:10:46 2010, 美东)
Please send in cover letter and CV to Nathan Lee: n**********[email protected]
Postdoctoral Positions in Pfizer Oncology, La Jolla CA
The post doctoral positions will be responsible for elucidating the
mechanism of action of existing Pfizer
portfolio compounds, ensuring that we have the appropriate patient selection
strategies for such
mechanisms, aligning ... 阅读全帖 |
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C**R
发帖数: 1047 | 21 http://gwtoday.gwu.edu/learningresearch/significantscience
highlight: "Mr. Zang’s research in biophysics and computational biology has
been published in nine publications, including Cell, Science, and Nature
Genetics, and he received the Best Poster Award in the 7th Asia-Pacific
Bioinformatics Conference."
Significant Science
GW Ph.D. candidate wins $4,000 award for his dissertation research.
Nov. 11, 2010
A George Washington University Ph.D. candidate has received a prestigious
science award fo... 阅读全帖 |
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b*******1
发帖数: 127 | 22 I agree that there are still a lot of noise in the data due to the platform,
and this is why some people on this board are cautious about bioinformatics
. However, I believe that when you have hundreds and thousands of data, you
can find the gold from the noise. The most important thing is the validation
! Don't stop at the prediction. It is our task to convince people that we
can find clinically useful diagnostics biomarkers/drugs from these high-
throughput data, so the world will follow. Beca... 阅读全帖 |
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g*********d
发帖数: 233 | 24 交大招的法国Nobel奖原来是去搞伪科学的
http://www.btsmth.com/show_snapshot.php?en_name=TsinghuaCent&gid=456861
Science诺奖得主吕克·蒙塔尼访谈
French Nobelist Escapes "Intellectual Terror" to Pursue Radical Ideas in
China
Martin Enserink
PARIS—Virologist and Nobel laureate Luc Montagnier announced earlier
this month that, at age 78, he will take on the leadership of a new
research institute at Jiaotong University in Shanghai. What has shocked
many scientists, however, isn't Montagnier's departure from France but
what he pla... 阅读全帖 |
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d****t
发帖数: 139 | 25 Expert Review of Molecular Diagnostics
January 2010, Vol. 10, No. 1, Pages 49-64
Title;
Discovery of prostate cancer biomarkers by microarray gene expression
profiling
Email: d****[email protected]
Thank you very much! |
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w******e
发帖数: 1187 | 26 thx for sharing.这倒是一个新思路，跟ruoslahti最近一篇science有点像。
不过蛋白酶的位点有很diverse吗？他们又要做basic science（validation of
protease
as cancer biomarker）又要做应用的话还挺苦的。不用Ab，总需要什么其他的
affinity
agent吧？
有没有相关paper推荐一下？thx~ |
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w******e
发帖数: 1187 | 27 poly arg。。。又见CPP啊呵呵。多谢解释。现在的问题只剩下了protease
activity是否足够特异。隐约记得MMPs有很多跟cancer相关，不过biomarker
的validation向来是瓶颈吧。当然，从发paper的角度来讲挺好的了。 |
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w******e
发帖数: 1187 | 28 这点还挺counter intuitive的。不知道他们用什么formulation。如果是NP，liver应该
是个主要的目的地（所以人们才质疑RNAi在肝脏以外能否work）。如果只是peptide，
renal clearance应该很厉害吧。and居然能过BBB，不知道有没有加其他料。印象中
有某peptide能过BBB但不记得是什么了，也不记得有没有paper跟进。
我个人理解medical imaging是否成功，一个是signaling motif要NB：无毒，yield高，
不受autofluorescence影响，穿透性好，etc；一个是targeting motif要NB：足够特异，
target level够高，最好affinity还能恰到好处，half life能控制好。这两方面我觉得
都不容易解决吧。fluorescence在background、穿透性等方面有局限吧？当然这方面如果
tsien组估计最有实力解决。target方面validated biomarker忒少了，
用于imaging，只有一层specificity check也有点安全隐患。
最后... 阅读全帖 |
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w******e
发帖数: 1187 | 29 测biomarker OE的话，ELISA不够精确？还是ab在lysate里不work？还是
有OE的细胞占整体比重太少？感觉FISH对操作的要求太高啊呵呵
另外请教imaging牛人，为啥还没有nb 软件能代替人眼看FISH、IF image呢？
我哥们前段时间做ctc staining，整天对着显微镜简直要瞎 |
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b****r
发帖数: 17995 | 30 biomarker OE是啥，over expression？那和FISH有啥关系啊
fish不是看染色体的吗，怎么和蛋白搅和到一起了，你说的神马俺都看不懂诶
FISH有软件的吧我记得。只是如果只是偶尔用一下，graduate student比软件便宜多了 |
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b******n
发帖数: 80 | 31 楼上的，我 查过了HPV-18是病毒。
我做了一个toxin，之前他是没有毒性的，然后我想注射到组织内，想用cancer特有的
蛋白的antibody连上，然后利用在局部区间高溶度，是的toxin进入细胞内，也就是穿
刺，最后将保护toxin的外壳去掉，利用toxin的来杀死cancer cell.所以我的问题是：
什么的蛋白是特有的biomarker for hela cell or MCF some other cancer cell? |
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q*******1
发帖数: 2 | 32 please see below detailed JD:
Executive Director of Discovery Biology
Location: Beijing
Key activities: Oversee biology-related research and drug discovery
activities to execute departmental strategies and meet deliverables.
Successful candidate is also responsible for biology resource allocation,
recruitment and the professional development of biology staff.
Requirements:
• Ph.D. in biology, cell biology, immunology, biochemistry,
virology, pharmacology or a related field with an ou... 阅读全帖 |
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Y*Z
发帖数: 1301 | 34 Clin Invest Med. 2007;30(5):E183-91.
Measurement of cortisol in human hair as a biomarker of systemic exposure.
Sauvé B, Koren G, Walsh G, Tokmakejian S, Van Uum SH.
SourceDiv. of Clinical Pharmacology and Toxicology, The Hospital for Sick
Children, Toronto, Canada.
Please send it to
l***********[email protected]
Thanks a lot |
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H**********J
发帖数: 351 | 35 国内各大公司都有Translational Medicine，但是Novartis是做Biomarker的，AZ是做
项目管理的，Roche开始招Leader，但一定要MD背景的，要很强的Disease的知识（肿瘤
、病毒、代谢），要有从Pre-clinical到Clinical的经验。美国什么情况？怎么定义？
有罗氏要求的人吗？谢谢大牛们！ |
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h*******o
发帖数: 4884 | 36 其实没什么,一个新热门词汇而已,大家都用这个去骗钱
说到底就是能把basic science discoveries 用到疾病中去,
举个例子,比如disease A 发现了一个新的target,和这个疾病发生很相关,
那translational 研究就跟上了
1) 根据这个target,有没有可能develop新的biomarker
2) 这个target是不是drugable, 发现了些candidates, 下面就开始做preclinical
efficacy---->preclinical safety--->Pk/PD---> IND--> clinical
其实都是换汤不换药.
然后最近还流行reverse translational research,顾名思义
就是translational research 上到human patients了,发现了什么问题
反过去优化知道basic science |
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w********e
发帖数: 110 | 37 A postdoctoral fellow is needed immediately in the Signal Transduction
Laboratory, Department of Gynecology and Obstetrics, in collaboration with
Dept of Neurology, Barrow Neurological Institute at St. Joseph’s Hospital
and Medical Center located in Phoenix, Arizona. The research in our
laboratories is focused on signal transduction pathways in cancers, diabetes
, and hypothalamic hamartoma (HH). Cell culture systems, animal models, as
well as specimens from human subjects are routinely used in... 阅读全帖 |
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R***c
发帖数: 13 | 38 【 以下文字转载自 Statistics 讨论区 】
发信人: Relic (铛铛铛铛铛), 信区: Statistics
标 题: Bioinformatician position at Shanghai, China
发信站: BBS 未名空间站 (Fri Jul 29 09:55:36 2011, 美东)
Position Title: Scientist I, Bioinformatics
Job Description:
Novartis Institute for Biomedical Research (NIBR)
is a world leader in pharmaceutical research with
a widely recognized culture for nurturing innovation
and scientific excellence to address unmet medical needs.
China NIBR based in Shanghai is dedicated to the
research and develop... 阅读全帖 |
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H**********J
发帖数: 351 | 39 我在招聘Assay Head/DMPK Head/Translational Medicine Leader/Biomarker/统计等
等 |
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w****s
发帖数: 235 | 40
in
what has been worked out for "clinical proteomics"? biomarker discovery was
a joke long time ago ,now the instrument gets more sensitive but dynamic
range is still a major challenge..I heard Ruedi was working on the topic he
knows well how to sell ideas but I did not hear anything exciting.any solid
idea that one could do with clinical proteomics? |
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K******S
发帖数: 10109 | 41 there is actually a little bit of history about NCI's CPTC. This time is
actually the 2nd phase. PhaseI also funded 5 sites, the goal was trying to
standardize the filed. But with so much money, most ppl from PhaseI just
took the money and did whatever they wanted. So PhaseI is not very
successful. (that's why only 2 sites from PhaseI got renewed to continue to
Phase II). Phase II is to focus on real clinical samples (several types of
tumor tissue samples), aka the real biomarker discovery phase... 阅读全帖 |
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h*******o
发帖数: 4884 | 42 In this case, the trial was flawed not because of the "double-blind" design,
but lack of valid surrogate endpoint or predictive biomarkers.
4 |
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D*a
发帖数: 6830 | 43 谁能讲讲那么一堆找biomarker的怎么就他的被广泛采用了？ |
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s******s
发帖数: 13035 | 44 啥叫就他一个。搞biomarker做clinical trial的无数多了 |
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D*a
发帖数: 6830 | 45 俺一点也不懂啊，biomarker到临床周期这么短么？搞出一个来上一个？ |
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s******s
发帖数: 13035 | 46 药还是老药，只是根据biomarker选哪个更有效罢了。我看phase0/1/2都可以
skip了，直接上3就可以了 |
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D*a
发帖数: 6830 | 47 谁能讲讲那么一堆找biomarker的怎么就他的被广泛采用了？ |
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s******s
发帖数: 13035 | 48 啥叫就他一个。搞biomarker做clinical trial的无数多了 |
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D*a
发帖数: 6830 | 49 俺一点也不懂啊，biomarker到临床周期这么短么？搞出一个来上一个？ |
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s******s
发帖数: 13035 | 50 药还是老药，只是根据biomarker选哪个更有效罢了。我看phase0/1/2都可以
skip了，直接上3就可以了 |
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