z*h
发帖数: 773 | 1 In the viewpoint of (traditional) engineer, Palsson's work has little
application potential. It is why Metabolic Engineering Father JE Bailey
wrote a paper for expressing his doubt (Bailey JE. Mathematical modeling and
analysis in biochemical engineering: past accomplishments and future
opportunities. Biotechnol Prog. 1998;14:8-20.). Now Palsson and others just
open a new pit and put water in. They cannot change anything big. Any
living systems are so complicated beyond engineers and scientist... 阅读全帖 |
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x**********8
发帖数: 190 | 2 Milestone NB, Bibby DM (1981) Concentration of alcohols by
adsorption on silicalite. J Chem Technol Biotechnol 31
x**********[email protected] |
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z*h
发帖数: 773 | 3 The use of metabolic model for simulation of complicated biological system
is a day dream based on available knowledge. The father of metabolic
engineering -- JE Bailey -- pointed it out in 1998.
Bailey, J. E. (1998) Mathematical modeling and analysis in biochemical
engineering: past accomplishments and future opportunities, Biotechnol. Prog
. 14, 8-20.
Who are really hearing his suggestions? In vivo synthetic biology for
biofuels production will be a dead end surely because of poor efficiencies... 阅读全帖 |
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z*h
发帖数: 773 | 4 The use of metabolic model for simulation of complicated biological system
is a day dream based on available knowledge. The father of metabolic
engineering -- JE Bailey -- pointed it out in 1998.
Bailey, J. E. (1998) Mathematical modeling and analysis in biochemical
engineering: past accomplishments and future opportunities, Biotechnol. Prog
. 14, 8-20.
Who are really hearing his suggestions? In vivo synthetic biology for
biofuels production will be a dead end surely because of poor efficiencies... 阅读全帖 |
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S*****s
发帖数: 287 | 5 不好意思我的说法有点误导。我的 BAC 上面通过 Cre-LoxP 反应又连上去了一个单独
表达的 GFP 质粒。我要表达的基因用 BAC 上自带的 promoter 表达，GFP 用质粒上带
的 SV40 promoter 表达，两个不干扰，但是只要有 GFP 的细胞一定会有我的 BAC DNA
。如果你感兴趣的话可以看看这篇文章
Wade-Martins R, Smith ER, Tyminski E, Chiocca EA, Saeki Y (2001) An
infectious transfer and expression system for genomic DNA loci in human and
mouse cells. Nat Biotechnol 19:1067-1070.
Dr. Saeki 人很好，想用这个质粒的朋友可以直接和他联系。pEHHG 质粒拿回来之后只
需要用 NEB 买来的 Cre 做体外反应就可以连上 BAC。 |
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a********e
发帖数: 1181 | 6 Mark Rank Abbreviated Journal Title
(linked to journal information) ISSN JCR Data More Information
EigenfactorTM MetricsMore Information
Total Cites Impact
Factor 5-Year
Impact
Factor Immediacy
Index Articles Cited
Half-life EigenfactorTM
Score Article InfluenceTM
Score
1 CA-CANCER J CLIN 0007-9235 9801 94.262 70.216
8.667 18 3.8 0.04923 24.782
2 ACTA CRYSTALLOGR A 0108-7673 13944 54.3... 阅读全帖 |
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g***j
发帖数: 40861 | 7 J Mol Microbiol Biotechnol. 2008;14(1-3):115-22.
Characterization of cecal microbiota and response to an orally administered
lactobacillus probiotic strain in the broiler chicken.
Gérard P, Brézillon C, Quéré F, Salmon A, Rabot S.
s*****[email protected]
thanks |
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s********s
发帖数: 67 | 8 paper 求助，非常感谢！
Microb Biotechnol. 2011 Nov;4(6):700-709. doi: 10.1111/j.1751-7915.2010.
00236.x. Epub 2011 Jan 6.
Microbiology of nitrogen cycle in animal manure compost. |
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i*****i
发帖数: 154 | 9 Nat Biotechnol. 2004 May;22(5):589-94. Epub 2004 Apr 4.
Correction of multi-gene deficiency in vivo using a single 'self-cleaving'
2A peptide-based retroviral vector.
Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin EF, Vignali
DA. |
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a**s
发帖数: 177 | 10 Nat Biotechnol. 2006 Dec;24(12):1558-64
Gene Ther. 2009 Oct;16(10):1189-201.
my email address is: h********[email protected]
thanks. |
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E********8
发帖数: 22 | 11 possible publication list for people graduated since 2010
118 伊成器
1. N6-methyladenosine in nuclear RNA is a major substrate of the obesity-
associated FTO.
Jia G, Fu Y, Zhao X, Dai Q, Zheng G, Yang Y, Yi C, Lindahl T, Pan T, Yang YG
, He C.
Nat Chem Biol. 2011 Oct 16;7(12):885-7. doi: 10.1038/nchembio.687.
2.Targeting MgrA-mediated virulence regulation in Staphylococcus aureus.
Sun F, Zhou L, Zhao BC, Deng X, Cho H, Yi C, Jian X, Song CX, Luan CH, Bae T
, Li Z, He C.
Chem Biol. 2011 Aug 26;18(8)... 阅读全帖 |
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l**********1
发帖数: 5204 | 12 Exactly.
FBS good or not good for your experiment is Lot number dependently.
please refer
Proteomic analysis for the assessment of different lots of fetal bovine serum as a raw material for cell culture.
Part IV. Application of proteomics to the manufacture of biological drugs.
Biotechnol Prog. 2006 Sep-Oct;22(5):1294-
/www.ncbi.nlm.nih.gov/pubmed/17022666
one |
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n******t
发帖数: 1281 | 13 FYI:
http://www.bacillusbbs.com/
Bacillus subtilis strain with inducible super-competence.
Zhang, X. Z. and Y. H. Zhang. 2011. Simple, fast and high-efficiency
transformation system for directed evolution of cellulase in Bacillus
subtilis. Microb. Biotechnol. 4:98-105. PMID: 21255377 read online
枯草杆菌感受态细胞的制备及转化
1) 将菌种接种在LB平板上37 ℃培养过夜。
2) 用接种环接一环菌苔于5mL GM I 溶液，在30 ℃慢摇床（125 r/min）振荡培养
过夜。
3) 次日取2 mL转接到18 mL GM I 中，37 ℃快摇床（250 r/min）培养3.5 h。
4) 再取10 mL上一步骤的培养液转接到90 mL GM II 中，37 ℃慢摇床培养9... 阅读全帖 |
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s******a
发帖数: 252 | 15 So it's Barkai's student.
1 Science, 1 Nat Genetics.
That's outstanding but not surprising.
Publications:
Tsui K, Dubuis S, Gebbia M, Morse RH, Barkai N, Tirosh I, Nislow C (2011).
Evolution of nucleosome occupancy: conservation of global properties and
divergence of gene-specific patterns. Mol Cell Biol. 31:4348-55.
Dori-Bachash M, Shema E, Tirosh I (2011). Coupled evolution of transcription
and mRNA degradation. PLoS Biol. 9(7):e1001106.
Tirosh I, Barkai N (2011). Inferring regulatory mechani... 阅读全帖 |
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w******y
发帖数: 2504 | 16 Could any friend send me a copy of the fllowing paper to purplewindxxh@
hotmail.com?
Curr Pharm Biotechnol. 2006 Apr;7(2):101-8.
Oxidative stress on progenitor and stem cells in cardiovascular diseases.
Yao EH, Yu Y, Fukuda N.
Thanks so much. |
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l**********1
发帖数: 5204 | 17 So what?
Is he (your PI) took left or right pathway? from below attached figure
from below b) nature sister?
a)
Huang S. (2009)
Non-genetic heterogeneity of cells in development: more than just noise.
Development.136: 3853-3862.
//www.ncbi.nlm.nih.gov/pubmed/19906852
b)
Strohman RC (1997)
The coming Kuhnian revolution in biology.
Nat Biotechnol. 15: 194-200.
//www.ncbi.nlm.nih.gov/pubmed/9062910
//bialystocker.net/files/kuhn.pdf |
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l**********1
发帖数: 5204 | 18 if originally omitted below attached figure shown right pathway
then no way can bet below report:
Levy SF et al.(2012)
Bet hedging in yeast by heterogeneous, age-correlated expression of a stress
protectant.
PLoS Biol. 10: e1001325.
Abstract
Genetically identical cells grown in the same culture display striking cell-
to-cell heterogeneity in gene expression and other traits. A crucial
challenge is to understand how much of this heterogeneity reflects the noise
tolerance of a robust system and ho... 阅读全帖 |
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l**********1
发帖数: 5204 | 19 LZ pls try long hairpin RNA (lhRNA)
Reference:
Tomimoto K et al. (2012)
Construction of a long hairpin RNA expression library using Cre recombinase.
J Biotechnol. 160: 129-39.
its pp130 left column
Functional screening using lhRNA expression libraries also appears to
be more efficient than using shRNA expression libraries for these
organisms.
link:
//www.ncbi.nlm.nih.gov/pubmed/22484114 |
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n***3
发帖数: 663 | 20 Nat Biotechnol. 2012 Aug 26. doi: 10.1038/nbt.2344. [Epub ahead of print]
Polyethyleneimine is a potent mucosal adjuvant for viral glycoprotein
antigens.
please send the paper to e*********[email protected]
Thanks! |
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l**********1
发帖数: 5204 | 21 LZ can try long hairpin RNAi
Reference:
Tomimoto K et al. (2012)
Construction of a long hairpin RNA expression library using Cre recombinase.
J Biotechnol. 160: 129-39.
PubMed link:
http://www.ncbi.nlm.nih.gov/pubmed/22484114
发信人: Wanshington (Joudon), 信区: Biology
标 题: Re: 讨论：信号通路上的蛋白酶功能研究策略哪种好？
发信站: BBS 未名空间站 (Fri Sep 28 03:33:37 2012, 美东)
RNAi不是有脱靶的问题吗？ 以前没有做过，所以对这个问题是否严重没有很好的把握
。这个蛋白的磷酸化位点不能突变成模拟磷酸化的蛋白，所以这条道路不通。 |
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l**********1
发帖数: 5204 | 22 Agree with sunny said.
btw, for 肽类analog from mushrom
pls refer
one 2011 review:
by
Wasser SP (2011)
Current findings, future trends, and unsolved problems in studies of
medicinal mushrooms.
Appl Microbiol Biotechnol. 89: 1323-32.
Its PubMed link:
http://www.ncbi.nlm.nih.gov/pubmed/21190105
and for 肽类analog modification to anti-tumor drugs
pls refer,
by
Iwasaki T et al. (2009)
Selective cancer cell cytotoxicity of enantiomeric 9-mer peptides derived
from beetle defensins depends on negatively ... 阅读全帖 |
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l**********1
发帖数: 5204 | 23 microfluidic platforms plus nanopore
pls refer
1)
Choi JW et al. (2012)
High-Throughput Analysis of Protein–Protein Interactions in Picoliter-
Volume Droplets Using Fluorescence Polarization
Anal. Chem. 84: 3849–3854
http://pubs.acs.org/doi/abs/10.1021/ac300414g
2)
Rudenko MI et al. (2011)
Controlled gating and electrical detection of single 50S ribosomal subunits
through a solid-state nanopore in a microfluidic chip.
Biosens Bioelectron. 29: 34-9.
http://www.ncbi.nlm.nih.gov/pubmed/21855314
3)
... 阅读全帖 |
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m****e
发帖数: 489 | 24 Biotechnol Lett. 2012 Apr;34(4):627-33. Epub 2011 Dec 9.
PolyA RT-PCR-based quantification of microRNA by using universal TaqMan
probe.
Luo X, Zhang J, Wang H, Du Y, Yang L, Zheng F, Ma D.
Can you pls send to a********[email protected]? MANY THANKS!! |
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l**********1
发帖数: 5204 | 25 Here you go, by bottom to up way: 以下括号内是按地区规模的比喻
Bibliography:
1, Deep Sequence Analysis of Non-Small Cell Lung Cancer: Integrated
Analysis of Gene Expression, Alternative Splicing, and Single Nucleotide
Variations in Lung Adenocarcinomas with and without Oncogenic KRAS Mutations
. (2012) (Newport beach, CA)
by
Kalari KR et al. and Thompson EA.
Front Oncol. 2:12. doi: 10.3389/fonc.2012.00012. Epub 2012 Feb 10.
Abstracts:
Our study is the first to integrate genomic features from RNA-S... 阅读全帖 |
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n***3
发帖数: 663 | 26 Nat Biotechnol. 2012 Nov 25. doi: 10.1038/nbt.2436.
Protective efficacy of in vitro synthesized, specific mRNA vaccines against
influenza A virus infection.
Please send the paper to e*********[email protected]
Thanks! |
|
J****n
发帖数: 156 | 27 Harvard E-journals竟然没有，有达人能帮我找找这篇文章吗？万分感谢。
J Biotechnol. 2012 Aug 31;160(3-4):146-50. doi: 10.1016/j.jbiotec.2012.02.
015. Epub 2012 Mar 21.
A simplified method for constructing artificial microRNAs based on the osa-
MIR528 precursor. |
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C******D
发帖数: 5 | 28 I would like to find the following 2 papers:
Holt S, Shay J, Wright W. Refining the telomere-telomerase hypothesis of
aging and cancer. Nat Biotechnol. 1996;14(7):836-836-9.
Multani A, Li C, Ozen M, Imam A, Wallace S, Pathak S. Cell-killing by
paclitaxel in a metastatic murine melanoma cell line is mediated by
extensive telomere erosion with no decrease in telomerase activity. Oncol
Rep. 1999;6(1):39-39-44.
Thank you very much! |
|
C******D
发帖数: 5 | 29 I would like to find the following 2 papers:
Holt S, Shay J, Wright W. Refining the telomere-telomerase hypothesis of
aging and cancer. Nat Biotechnol. 1996;14(7):836-836-9.
Multani A, Li C, Ozen M, Imam A, Wallace S, Pathak S. Cell-killing by
paclitaxel in a metastatic murine melanoma cell line is mediated by
extensive telomere erosion with no decrease in telomerase activity. Oncol
Rep. 1999;6(1):39-39-44.
Thank you very much! |
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A****e
发帖数: 1165 | 30 求
Curr Pharm Biotechnol. 2012 Jun 1.
The Warburg Hypothesis and the ATP Supply In Cancer Cells Is Oxidative
Phosphorylation impaired in malignant neoplasias?
Thanks a lot
Please send it to a****[email protected] |
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l**********1
发帖数: 5204 | 31 GST-A.B co-expression pls let IPTG and the cultures were maintained at 4°C
for 48-72 h then do that induction
reference:
PMID 23525091
Production of soluble eukaryotic recombinant proteins in E. coli is favoured
in early log-phase cultures induced at low temperature. (2012).
Springerplus. 2:89.
FINDINGS:
A high yield of active soluble proteins was obtained by combining early-log
phase cultures and low temperatures for protein induction. When IPTG was
added at OD600 = 0.1 and cultures... 阅读全帖 |
|
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d******n
发帖数: 29 | 33 Split intein mediated ultra-rapid purification of tagless protein (SIRP).
Guan D, Ramirez M, Chen Z.
Biotechnol Bioeng. 2013 Apr 8.
谢谢！！第一个发的有包子，不好意思，穷人包子不多。
请发到d******[email protected] |
|
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M****e
发帖数: 262 | 35 这人的简历，也有些奇葩啊
http://www.caufood.com.cn/show-zhuanjia.asp?%5C=606
姓名 朱毅
性别 女
出生日期 1973年6月
籍贯 贵州
民族 汉
学历/学位 博士
职称 副教授学习工作经历
1989-1994, 贵阳医学院，医学士
1994-2001，贵阳医学院，助教，讲师，基础医学部、临床医学院团委书记
1999-2002，武汉大学，法硕士
2003-2006，中科院植物所，理博士
2006-今 中国农业大学食品学院，讲师现任职务（社会兼职等）主要研究方向
果蔬采后保鲜与生物活性物质功效研究
微藻综合利用生物技术代表著作与论文
Y. Zhu, et al., 2007, A simple method for extracting C-phycocyanin from
Spirulina platensis using Klebsiella pneumoniae, Appl Microbiol Biotechnol,
74: 244-248 (第一作者)
提高衣藻放氢效率的方法，专利... 阅读全帖 |
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M****e
发帖数: 262 | 36 医学士，团委书记，法学硕士（在职拿的），植物学博士，代表论文是水论文一篇（还
是博士毕业后才发的）
看朱毅这简历，明眼人一眼就可以看出有猫腻。要靠博士学位混饭吃，这女人却还这样
胆大，要去惹方舟子。
这真是找死啊。
http://www.caufood.com.cn/show-zhuanjia.asp?%5C=606
姓名 朱毅
性别 女
出生日期 1973年6月
籍贯 贵州
民族 汉
学历/学位 博士
职称 副教授学习工作经历
1989-1994, 贵阳医学院，医学士
1994-2001，贵阳医学院，助教，讲师，基础医学部、临床医学院团委书记
1999-2002，武汉大学，法硕士
2003-2006，中科院植物所，理博士
2006-今 中国农业大学食品学院，讲师现任职务（社会兼职等）主要研究方向
果蔬采后保鲜与生物活性物质功效研究
微藻综合利用生物技术代表著作与论文
Y. Zhu, et al., 2007, A simple method for extracting C-phycocyanin from
Spirulina plat... 阅读全帖 |
|
p*****c
发帖数: 20445 | 37 Cell. 2013 Aug 29
Nature. 2013 Aug 22
Nat Biotechnol. 2013 Jul 21
Science. 2013 Feb 15
Nat Commun. 2012 Jul 24 |
|
c****y
发帖数: 373 | 38 "FSA 人类食用试验——2004 出版的 Newcastle 大学为“食品标准代理”进行的是唯
一的人类转基因食物试验。实验设计为研究人体胃部的转基因 DNA 是排泄还是进入体
内的细 菌,这是一长期备受关注的问题。研究发现,转基因大豆中的全部基因通过胃和
小肠时能全 部存活,但是没有通过直肠。研究也发现部分转基因 DNA 从转基因食物中
“水平”转移到 肠胃中的自生细菌,这一震撼性的研究可能涉及食用转基因的长期影响
16,28。但正是由于 这一研究的震撼性,当时 FSA 在学术交流时选择绝口不提这一发现
,因而造成公众认为在 这项研究中并没有发现基因水平迁移的影响。"
这个应该是这篇文章吧
Netherwood T, Martín-Orúe S.M, O'Donnell A.G, et al. Assessing the
survival of transgenic plant DNA in the human gastrointestinal tract. Nat
Biotechnol. 2004;22(2):204–209.
文章的摘要最后一句是
As this low lev... 阅读全帖 |
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l**********1
发帖数: 5204 | 39 sure 楼主 还是 做Cre rabbit 吧
一直最后做到Cre Monkey or even Ape ? 超过Bill Gates 哈
just check one table,
cited from
PMID 22665989
J Biomed Biotechnol. (2012). 2012: 692609.
Animal models of glaucoma.
http://www.ncbi.nlm.nih.gov/pubmed/22665989
breeding |
|
h***n
发帖数: 83 | 40 【 以下文字转载自 Military 讨论区 】
发信人: hzhen (hzhen), 信区: Military
标 题: 方舟子：驳斥中科院植物所对朱毅问题的说明
发信站: BBS 未名空间站 (Tue Apr 29 21:35:39 2014, 美东)
http://fangzhouzi.baijia.baidu.com/article/13752
驳斥中科院植物所对朱毅问题的说明
方舟子04月29日 16:45 分享到： 朱毅 学术造假 分类 : 热点
摘要 : 拔出萝卜带出泥，一个水货、假货，尽显中国科学界之丑陋，之腐败。
我在4月23日发出《就农大朱毅问题致中国科学院院长、中国农业大学校长的公开信》
（http://fangzhouzi.baijia.baidu.com/article/13040 ）后，至今未收到答复，其间朱毅继续对我谩骂并扬言要起诉我：
【方舟子今天又耍无赖继续玩告状的招数，一而再，再而三，中科院，农大，都去招摇
闹腾了。我招惹来社会流氓，给中科院和农大领导添麻烦了，但诸位不必为我费心劳神
，小女子将坦然面对，走法律途径还大家一个清静。方舟子，我忍你很... 阅读全帖 |
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h***n
发帖数: 83 | 41 【 以下文字转载自 Military 讨论区 】
发信人: hzhen (hzhen), 信区: Military
标 题: 方舟子：举报季宏兵、朱毅夫妇互送论文
发信站: BBS 未名空间站 (Sat May 3 10:16:52 2014, 美东)
http://fangzhouzi.baijia.baidu.com/article/14105
举报季宏兵、朱毅夫妇互送论文
方舟子05月02日 19:28 分享到： 朱毅 学术造假 分类 : 热点
摘要 : 向国家自然基金委员会举报季宏兵、朱毅夫妇互送论文。
夫季宏兵，1990年毕业于长春地质学院，1993获得中国科学院地球化学研究
所理学硕士，2000年获得该所博士，现为北京科技大学环境工程系教授、中国科学院地
球化学研究所研究员、首都师范大学资源环境与旅游学院研究员、博士生导师，入选北
京市新世纪百千万人才工程、中国科学院百人计划。研究方向为环境科学、环境生物地
球化学。
妇朱毅，1994年毕业于贵阳医学院临床医学专业，留校当团干，2002年在职获得武汉大
学马克思主义思想教育专业法硕士，2006年获得中... 阅读全帖 |
|
w*********n
发帖数: 439 | 42 Nat Biotechnol. 2014 Jun;32(6):569-76.
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.
Tsai SQ1, Wyvekens N2, Khayter C2, Foden JA2, Thapar V3, Reyon D1, Goodwin
MJ2, Aryee MJ4, Joung JK1. |
|
s***a
发帖数: 43 | 43 【 以下文字转载自 Faculty 讨论区 】
发信人: SWJSJ (BC), 信区: Faculty
标 题: 调研了一下清华最近回去的PI
发信站: BBS 未名空间站 (Mon Dec 15 16:27:25 2014, 美东)
云老师发了个贴说清华目前的找人标准，很多人不相信。特地调研了一下清华生命学院
最近回去的人的情况，如云老师所说，绝大多数都有2cns，如果只有一篇cns，那基本
上还有>2的子刊。这个不仅仅局限于胖老师经常说的结构生物学，而是所有方向都基本
如此。
当然这些新回去的绝大部分都是千青（有些不是的是因为回去那一年千青还没开始）。
网页上的Title有的是教授，有的是研究员，估计当时还没有统一。但校内一律都是副
教授，也就是相当于这边的AP。我还特地打听了一下，国内的TT制度好像没有Tenured
Associate这一档，清华5年考核通过了就是正经的full professor。
有些老师看不上国内的科研，我就仅列出两位回国后的publication，咱就不说颜宁等
这种Early T的个例了。说两个没太有名气的，还没有评上T的，各位老师可以参考一下
，... 阅读全帖 |
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b*****s
发帖数: 382 | 45 Chi Z, Kohlwein SD, Paltauf F. 1999. Role of phosphatidylinositol (PI) in
ethanol production and ethanol tolerance by a high-ethanol-producing
yeast. J. Ind. Microbiol. Biotechnol. 22:58–63.
[email protected]
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谢谢！ |
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m********2
发帖数: 317 | 46 Nat Biotechnol. 2014 Sep;32(9):941-6. doi: 10.1038/nbt.2951. Epub 2014 Jun
22.
Generation of mouse models of myeloid malignancy with combinatorial genetic
lesions using CRISPR-Cas9 genome editing.
有同学用过这篇文章提到过的质粒吗?
我用了，但是不workwork，包病毒的时候能看到293T细胞全部绿了，但是收集的病毒
titer非常非常低，根本不能重复文章中的转染效率。 |
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m********2
发帖数: 317 | 47 Nat Biotechnol. 2014 Sep;32(9):941-6. doi: 10.1038/nbt.2951. Epub 2014 Jun
22.
Generation of mouse models of myeloid malignancy with combinatorial genetic
lesions using CRISPR-Cas9 genome editing.
给通讯作者写信问了，然后第一作者的回复是这样的:
for the original virus we have been using standard calcium phosphate
transfection wit plasmid ratios of 6:10:1.5 (all ug per 10 cm dish) for
vector:psPAX2:pMD2.G.
The virus was collected at around 24-28h and 36-40h post transfection and
concentrated 50-100x with ultrazentrifuga... 阅读全帖 |
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l********n
发帖数: 260 | 48 1. Zhang P, Wei Y, Wang L, Debeb BG, Yuan Y, Zhang J, Yuan J, Wang M,
Chen D, Sun Y, Woodward W, Liu Y, Dean DC, Liang H, Hu Y, Ang KK, Hung MC,
Chen J, Ma L. ATM-mediated stabilization of ZEB1 promotes DNA damage
response and radioresistance through CHK1. Nat Cell Biol. In Press.
2. Chen D, Sun Y, Yuan Y, Han Z, Zhang P, Zhang J, You MJ, Teruya-
Feldstein J, Wang M, Gupta S, Hung MC, Liang H, Ma L. miR-100 induces
epithelial-mesenchymal transition but suppresses tumorigenesis, migration
a... 阅读全帖 |
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M*P
发帖数: 6456 | 49 Sequenom’s test, which uses DNA found in maternal blood samples to
determine certain fetal characteristics, avoids the risk of more invasive
tests, such as taking samples from
the fetus or placenta. The court ruled that detecting DNA in blood falls
under the US Supreme Court’s rule against patenting natural phenomena (Nat.
Biotechnol. 32, 403–404, 2014). While calling the test “a positive and
valuable contribution to science...[e]ven such valuable contributions
can fall short of statutory patent... 阅读全帖 |
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t*******k
发帖数: 87 | 50 比较偏技术的文章，不知那位大侠有全文的权限
Biotechnol Bioeng. 2011 Mar;108(3):600-10. doi: 10.1002/bit.22968. Epub 2010
Nov 23.
Generation of stable, high-producing CHO cell lines by lentiviral vector-
mediated gene transfer in serum-free suspension culture.
Oberbek A1, Matasci M, Hacker DL, Wurm FM.
信箱 [email protected]
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谢谢！ |
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