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全部话题 - 话题: broun
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x****6
发帖数: 4339
1
错你个头,这里是朵得娜2012年以前发表的跟crispr相关的文章,最早的是2010,张就
是看到这里的某些工作开始动手做crispr的:
Wiedenheft B, Lander GC, Zhou K, Jore MM, Brouns SJ, van der Oost J,
Doudna JA, Nogales E. (2011) Structures of the RNA-guided surveillance
complex from a bacterial immune system. Nature. Sep 21; 477(7365): 486-9.
PMID: 21938068

Sashital, DG, Jinek M, Doudna JA. (2011) An RNA-induced conformational
change required for CRISPR RNA cleavage by the endoribonuclease Cse3. Nat.
Struct. Mol. Biol. Jun; 18(6):680-7. PMID: 2... 阅读全帖
p**m
发帖数: 3876
2
By Ken Sweet and Liz Claman
FOXBusiness
Three executives at the privately-held electric car company Telsa Motors
have died in a plane crash Wednesday afternoon, but the company's founder
was not on board.
According to the car blog Jalopnik and the San Francisco Chronicle, a twin-
engine Cessna crashed a residential neighborhood in Palo Alto, Calif.
The plane was owned by Doug Broun, a senior electrical engineer with Telsa,
according to the Chronicle. But it was unclear if Broun was onboard or wh
m*******n
发帖数: 1214
3
来自主题: Biology版 - Why biology is not in STEM?
Found this post in law board
"Some people ask me why Biology is not included in STEM act, the answer is
simple most GOP leaders don’t believe in modern biology and evolution in
first place and house is controlled by GOP right now. 2016 potential GOP
president candidate Louisiana governor Bobby Jindal said Creationism should
be taught in public school for kids. WTF!!!!"
https://www.youtube.com/watch?v=huXANQi-8TM
ZZ
发信人: ICEROCKTI (JAKE), 信区: Law
标 题: GOP fact No.3-Science and Technology
发信站: BB... 阅读全帖
I*******I
发帖数: 195
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This is probably the most amusing fact about GOP leaders. Be prepared for a
bumpy ride, some of you probably won't believe this is true.
GOP claims they have respect for science and technology, while in fact GOP
leaders don’t believe in science in the first place. Let’s take a look at
what comes out the mouth of these GOP house members. And keep in mind these
are not your typical congressman, these Republicans are members of the House
Committee on Science, Space and Technology.
1.Rep. Paul Broun... 阅读全帖
s*********8
发帖数: 901
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来自主题: Military版 - US gov blocked pro-union website
Wis. Assembly passes bill taking away union rights
MADISON, Wis. — The Wisconsin Assembly early Friday passed a bill that
would strip most public workers of their collective bargaining rights — the
first significant action on the new Republican governor's plan.
Other political news of note Some Republicans soften tough talk on unions
With a wary eye on Wisconsin, Republican leaders in several states are
toning down the tough talk against public employee unions and, in some cases
, abandoning ant... 阅读全帖
z*****y
发帖数: 554
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来自主题: Military版 - 支持共和党的被当众打脸了!
共和党不是号称削减开支吗? 怎么实际上都是军火商的代理人? 其他地方再节省,不够
给军火商塞牙缝的.
House lawmakers voted 325-98 to pass a bill authorizing more than $590
billion for defense activities in fiscal 2015.
http://blogs.rollcall.com/218/house-passes-defense-authorizatio
共和党里只有4个议员投了反对票!!! 其他人都是是fraud, fake fiscal conservative
, 都给军火商输送利益的. 你们都支持他们这些RINO什么? 还不如奥巴马,至少奥巴马
知道并且承认自己是democrat!
这4根独苗都是RonPaul republican... 神马tea party favorite的怎么都支持花钱去
了? 怎么只有RonPaul的人才是真么表里如一的fiscal conservative?
这4个人里面Walter Jones of NC 和Thomas Massie of... 阅读全帖
d*b
发帖数: 4184
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【 以下文字转载自 USANews 讨论区 】
发信人: HIFU (花里胡哨), 信区: USANews
标 题: House Votes 225-201 Authorizing Lawsuit Against Obama
发信站: BBS 未名空间站 (Wed Jul 30 19:21:58 2014, 美东)
http://nation.foxnews.com/2014/07/30/house-votes-225-201-author
FOX News reports that the US House of Representatives has voted 225-201 on a
resolution authorizing a lawsuit against President Obama.
All Democrats and five Republicans voted 'nay'.
The Republicans voting no were said to be Reps. Scott Garrett (R-NJ), Paul
Broun (R-Ga.), Tho... 阅读全帖
p******e
发帖数: 897
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Members of the Congressional Tea Party Caucus may tout their commitment to
cutting government spending now, but they used the 111th Congress to request
hundreds of earmarks that, taken cumulatively, added more than $1 billion
to the federal budget.
According to a Hotline review of records compiled by Citizens Against
Government Waste, the 52 members of the caucus, which pledges to cut
spending and reduce the size of government, requested a total of 764
earmarks valued at $1,049,783,150 during Fi... 阅读全帖
H**U
发帖数: 1814
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http://nation.foxnews.com/2014/07/30/house-votes-225-201-author
FOX News reports that the US House of Representatives has voted 225-201 on a
resolution authorizing a lawsuit against President Obama.
All Democrats and five Republicans voted 'nay'.
The Republicans voting no were said to be Reps. Scott Garrett (R-NJ), Paul
Broun (R-Ga.), Thomas Massie (R-Ky.), Steve Stockman (R-Texas) and Walter
Jones, Jr. (R-N.C.)
Jones recently told The Hill that he planned to vote 'no' on the resolution
due to t... 阅读全帖
w**j
发帖数: 1032
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来自主题: Stock版 - vote is delayed again????

paulbip wrote:
I’m a lefty but I think Boehner tried hard. My Republican Congressman is
Paul Broun and he is a real a hole. Jesus is telling him to vote no so he
says. With people like that, who needs terrorists?
Jul 28, 2011 10:09pm EDT -- Report as abuse
c********r
发帖数: 649
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来自主题: EB23版 - HR 3012 money trail
http://www.opencongress.org/bill/112-h3012/money
Interests that support this bill:
Electronics manufacturing & services
Online computer services
Computer software
Computers, components & accessories
International trade associations
Chambers of commerce
Republican/Conservative
Minority/Ethnic Groups
Top recipients for ALL supporting interest groups
Name Amount Received Vote On Passage
Rep. Addison Wilson [R, SC-2] $201,431 Aye
Rep. Michele Bachmann ... 阅读全帖
b*****d
发帖数: 61690
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来自主题: EB23版 - 开始各个击破了
The Service Employees International Union is launching Spanish-language
radio ads aimed at pressuring a dozen House Republicans to support
immigration reform.
The $200,000 in ads target Republicans who represent a large number of
Hispanics, and were announced as the House GOP conference prepares for a
Wednesday meeting to discuss the way forward on immigration reform.
"The debate over immigration reform has reached its deciding moment," the ad
begins, before playing clips of Reps. Paul Broun (R-... 阅读全帖
p**r
发帖数: 109
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来自主题: Belief版 - 传教士与八国联军[zz]
【新语丝电子文库(www.xys.org)(www.xys2.org)】
————————————————
周延胜
1900年6月,英、法、美、德、日、俄、意、奥组成八国联军,发
动了大规模的侵华战争。8月14日,八国联军攻陷北京。在这里,他们
烧杀抢劫,奸淫虏掠,无恶不作,使中国几千年的文化成果遭到空前
破坏,千年古都顿成人间地狱,“尸积遍地,白骨纵横”。值得一提
的是,在这场罕见的人类大悲剧中,某些西方传教士充当了罪恶的帮
凶和直接的刽子手,他们完全背离了宗教的主旨,亵渎了“天主”的
“圣灵”,是人类文明史的耻辱。在历史面前,他们难辞其咎。下面
从几个侧面看看在八国联军侵华期间,西方传教士的所为。
首先,直接参加军事行为,第一个表现就是充当军事间谍,为侵
略军提供情报。在八国联军侵入天津时,英国籍传教士宝复礼(
Frederick Broun)接受了侵略军司令部的委任,正式编入军队,隶属
于军事情报局,成为“英国远征军”的成员。作为“向导”领着八国
联军从天津一直打到北京。他还驱使信徒搜集军事情报,如清军大炮
口径和数目,战壕、水雷和地雷情况,并在地图上标出;为侵略军修
筑战壕,把
l*******i
发帖数: 153
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注明位置调换的,应该可以吧,就像下面这位:
Lander GC*, Wiedenheft B*, Zhou K, Jore MM, Brouns SJ, van der Oost J,
Doudna JA, Nogales E. Structures of the RNA-guided surveillance complex from
a bacterial immune system. Nature 2011; 477(7365):486-9.
*Co-first Authors (order changed for emphasis)

发帖数: 1
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来自主题: Biology版 - CRISPR英雄谱
#注明译者,请随便转载:)
CRISPR英雄谱
埃里克・兰德(Eric Lander)
小鹿/译
三年之前,科学家们宣布,CRISPR技术能够对真核活细胞进行精准与有效的基因组编辑
。自此,这项技术手段已然震撼了科学界,数以千计的实验室正在将其运用于从生物医
药到农业的各个领域。然而,从一种奇特的细菌重复序列现象的发现开始,到确认这种
现象为适应性免疫系统,进而对它的生物功能特性的了解,直至开发为一项基因工程的
技术,这此前二十载相关的研究历程却不为人所知。本文正是着眼于填补这段科学历史
的空白,它讲述的是观念的演化历史和先锋人物的传奇故事,并且从中获得关于支撑科
学发现的优秀科研环境的启迪。
前言
很难想起曾经有哪一次科学革命像CRISPR这般如此迅速地改变生物学界。仅仅三年之前
,科学家宣布,CRISPR系统,即细菌通过纪录和精准攻击入侵病毒的DNA序列而进行自
身防御的适应性免疫系统,可以利用转化为一项简单而有效的技术在哺乳动物和其它生
物体的活体细胞内进行基因组编辑。CRISPR即此被全球数以千计的实验室运用于广泛的
领域,如创建人类遗传疾病和癌症的复杂动物模型;... 阅读全帖
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