x*****9
发帖数: 68 | 1 实验室一个博后教我的时候很简单
C18混合在丙酮中， 超声10分钟， 然后接上高压packing就好了
我自己在做的过程中经常有问题， 1）隔夜都不完全， 柱子里面C18都是一段一段的，
不用显微镜， 在外面都可以看得出来 2）就算是连续的C18， 毛细管内壁一侧， 断
断续续有黑黑的阴影， 感觉是脏东西， 不知道哪来的。 丙酮是HPLC级别的。
最后导致的问题是压力偏高， 我pack的柱子压力都要比教我的博后高几十。
有经验的说说， 我的问题在哪里？ |
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v********g
发帖数: 25 | 2 换hexane试试捏？有可能压力小点。
也有可能frit固化得太实了... |
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w***c
发帖数: 116 | 3 对，frit也可能导致pressure过高。尽量短点吧。 |
|
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t**n
发帖数: 4365 | 5 C18 suspension 的密度得小点，流速也低点。。。 |
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z***3
发帖数: 496 | 6 Journal of the Chemical Society, Transactions
Samuel Sugden
J. Chem. Soc., Trans., 1921, 119, 1483-1492
CLXXV.—The determination of surface tension from the rise in capillary
tubes |
|
z***3
发帖数: 496 | 7 I really need this paper before weekend...
Journal Title: Proceedings of the Royal Society London A
Volume: 92
Date of Publication: 1916
Pages: 184
Article Author/Ed.: Rayleigh
Article title: On the Theory of the Capillary Tube
Comment: Proc. R. Soc. Lond. A 1 January 1916 vol. 92 no. 637 184-195
thank you so much! |
|
b*****y
发帖数: 175 | 8 Thanks again for all your new inputs!
Additional information:
1. I used Vydac MS C18 capillary column.
2. The sequence coverage of my protein (from trypsin digested peptides MS
analysis) is consistent (80%) for both non-treated and treated samples. So
my confusion is since the protein identity is confirmed from peptide MS
analysis, why it does not show in protein MS analysis.
3. We do not have MALDI in the lab so it is hard to check using another MS
technique.
4. I found out the 28498 Da peak in... 阅读全帖 |
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a*****n
发帖数: 20 | 9 1) The pressure is normal. Either the column or pre-column is contaminated.
Change a new pre-column, or check whether the packing material slurry or the
pressure packing device was contaminated.
2) What concentration do you have? If the intensity is too low for standard,
you might need to clean the ion optics ( transfer capillary, S-lens, etc).
Orbi Velos needs to be cleaned very often due to its flawed ion optics
design. |
|
h***8
发帖数: 41 | 10 There is an intern position for capillary electrophoresis analysis of
protein therapeutics. Graduate students (PhD MS candidates) are welcome to
apply for. For more details, pls go
www.myBayerjob.com
Reference Code: 0000032724 |
|
|
h***o
发帖数: 350 | 12 Real-time detection of chemical warfare agent simulants in forensic samples
using active capillary plasma ionization with benchtop and field-deployable
mass spectrometers
Morphy Dumlao, Pablo Martinez-Lozano Sinues, Maryia Nudnova and Renato
Zenobi
Anal. Methods, 2014,6, 3604-3609
DOI: 10.1039/C4AY00303A
Link:
http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00303
Thank you very much. |
|
q****i
发帖数: 6923 | 13 Microporous and Mesoporous Materials
Volume 115, Issue 3, 1 November 2008, Pages 383–388
Synthesis and characterization of hollow zeolite microspheres with a
mesoporous shell by O/W/O emulsion and vapor-phase transport method
http://www.sciencedirect.com/science/article/pii/S1387181108000
Concentric Hollow Nanospheres of Mesoporous Silica Shell-Titania Core from
Combined Inorganic and Polymer Syntheses
Guoliang Li, E. T. Kang, K. G. Neoh and Xinlin Yang
Langmuir, 2009, 25 (8), pp 4361–4364
Publi... 阅读全帖 |
|
i*n
发帖数: 9793 | 14 J. Chamieh, C. Faye, V. Dugas, T. Moreau, O. Vandenabeele-Trambouze,C. Demes
may,
Preparation and full characterization of a micro-immunoaffinity monolithic c
olumn and its in line coupling with capillary zone electrophoresis with Ochr
atoxin A as model solute,
J.Chromatogr. A 1232 (2012) 93-100. |
|
发帖数: 1 | 15 Single-bead arrays for fluorescence-based immunoassays on capillary-driven
microfluidic chips
Yuksel Temiz ; Michel Lim ; Emmanuel Delamarche
Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV,
97050Z (March 21, 2016); doi:10.1117/12.2212121 |
|
x****o
发帖数: 60 | 16 【 以下文字转载自 JobHunting 讨论区 】
发信人: xiaoao (吃了睡,睡了再吃), 信区: JobHunting
标 题: analytical scientist opportunity (Boston area)
关键字: analytical job,Boston area,biopharma
发信站: BBS 未名空间站 (Sun May 15 14:01:14 2016, 美东)
A long-term contract job opportunity is available in the Boston area. The
person will work for a big biopharmaceutical company through a third party.
The Contract Personnel will have the following minimum requirements:
• BA or BS in Chemistry, Biochemistry, Biology, Microbiology or
e... 阅读全帖 |
|
W******r
发帖数: 789 | 17 By Mark A Ament
If you only make one improvement to your diet this year, I hope it is to
replace refined sugar with raw food sweeteners. If you do, I can guarantee
you that smoother digestion, more relaxed muscles, more peaceful emotions
and increased mental clarity are soon to follow.
Before looking at the negative health consequences of eating refined sugar,
let's have a quick look at how it's made. This will give you some insight
into why it affects the body the way it does. The majority of s... 阅读全帖 |
|
L**0
发帖数: 249 | 18 【 以下文字转载自 Medicine 讨论区 】
发信人: Ly80 (Jennifer), 信区: Medicine
标 题: Re: isotonic opc-3
发信站: BBS 未名空间站 (Sun Apr 21 15:54:08 2013, 美东)
Below is the product description. 没有人听说过这个product? 懂医的能不能讲讲
原理
Product Description
Isotonix OPC-3 is a superior source of antioxidant protection against free-
radical damage and premature aging. It includes a powerful combination of
bioflavonoids containing a superior source of Oligomeric Proanthocyanidins,
powerful antioxidants crucial in supporting the circulatory... 阅读全帖 |
|
a***a
发帖数: 974 | 19 I want to simulate the filling of viscous flow into a channel. Actually I work
on injection molding, but injection molding simulation software don't have a
good control on all the phenomena related with micro scale filling such as
capillary force. ANSYS seems not good on viscous flow. What else could I use? |
|
发帖数: 1 | 20 德克萨斯大学达拉斯分校机械系戴贤明教授课题组 (8月入职，参考simondai.weebly.
com) 全奖招收博士生。专业不限，机械、能源动力、电子、材料、化学、生物相关专
业都可考虑。具体方向为: (1) 热能利用, 传热传质或电池的热管理; (2) 超疏水/超
亲水表面微纳米加工或电化学合成; (3) 生物检测，生物传热或其它与生物工程相关的
方向。目前德州政府投资80亿美金致力于打造一批更高水平大学。德州大学达拉斯分校
是德州政府重点打造的公立大学，其目标是进入美国大学前50名，近几年区域经济十分
活跃，学校各项排名稳步上升。具体信息如下:
Multiple PhD positions in Mechanical Engineering with full financial support
are available starting from Fall 2016 or Spring 2017 in Prof. Xianming Dai'
s group (simondai.weebly.com) at The University of Texas, Dallas. Th... 阅读全帖 |
|
s****u
发帖数: 19 | 21 ~{!>~} ~{TZ~} wonderlich (~{Ws06#,6]H%~}) ~{5D4sWwVPLa5=~}: ~{!?~}
composites??
I am sure here are many da xia. and I have many questions too. :)
first: how do I select rheometer? I know basically there are two kinds:
capillary and oscillary.
second: I guess that we might can get some different result from testing
filled polymer system from pure polymer system. can you tell me what more
information I can get?
Thanks, |
|
t***r
发帖数: 3 | 22 try doing it in liquid if possible. To reduce capillary force in air from
absorbed water layer.
转
围
比
是
我
可
压
较
图 |
|
f******n
发帖数: 4 | 23 It's probably some instablity which chooses a wavelength of tens micrometers. Capilary force may be one factor. Do you have two balancing forces which give you a length of tens microns? |
|
w********h
发帖数: 12367 | 24 ATS RheoSystems/REOLOGICA Instruments
Rheology Staff Engineer
ATS RheoSystems/REOLOGICA Instruments are a comprehensive rheological
instrumentation and technical support company specializing in rheometer and
viscometer design, research level rheometers and rheology, capillary
rheometers,
rheological consulting, viscometers, and viscosity measurements, real-time
light
and thermal cure profiling, low and high pressure rheology measurements,
dynamic shear rheometers (DSR) for asphalt testing accord |
|
sc
发帖数: 122 | 25 md要什么文献阿，就是牛顿第二定律嘛，做的系统小，不用并行就更简单了
几乎没算法。
有一点统计物理的背景更好些。
如果做流体，推荐the art of molecular dynamics忘记是谁写的了
还有一本capillary....忘记名字了 |
|
sc
发帖数: 122 | 26 要是错了,全世界做md的都好去wyoming转行了.
另外,昨天没想起来的书名叫做:
molecular theory of capillary
你做流体还是固体?固体我就从此闭嘴了... |
|
j****x
发帖数: 943 | 27 This is a typical surface tension related problem. Because of the huge
curvature in capillaries, which is inversely proportional to the tube radius
, you could add surfactant on one side, and hopefully, the fluid inside will
move by itself towards the side without surfactant. Nothing else is
required. |
|
发帖数: 1 | 28 德克萨斯大学达拉斯分校机械系戴贤明教授课题组 (8月入职，参考simondai.weebly.
com) 全奖招收博士生。专业不限，机械、能源动力、电子、材料、化学、生物相关专
业都可考虑。具体方向为: (1) 热能利用, 传热传质或电池的热管理; (2) 超疏水/超
亲水表面微纳米加工或电化学合成; (3) 生物检测，生物传热或其它与生物工程相关的
方向。目前德州政府投资80亿美金致力于打造一批更高水平大学。德州大学达拉斯分校
是德州政府重点打造的公立大学，其目标是进入美国大学前50名，近几年区域经济十分
活跃，学校各项排名稳步上升。具体信息如下:
Multiple PhD positions in Mechanical Engineering with full financial support
are available starting from Fall 2016 or Spring 2017 in Prof. Xianming Dai'
s group (simondai.weebly.com) at The University of Texas, Dallas. Th... 阅读全帖 |
|
l******i
发帖数: 9 | 29 乡下表弟不幸得了 肝炎相关性肾炎／肾病综合症。来信求助。看了他的病理报告，多
处不懂。只好勉强将其译成英文，
想请有关的专家看看。先恳请各位病理医生帮助修改一下或给出出主意，咋治疗才能好
， 他人才只有27岁。拜谢了。
检查所见：
免疫荧光镜： 5个肾小球沿系膜区及毛细血管壁呈颗粒样分布：
Ig G-; IgM+ -; Ig A + -; C3 -; F +; C1q + -
Immunofluorescence Microscope: The five examined glomeruli are distributed
granulometrically along the
mesangium and capillary wall.
Ig G-; IgM+ -; Ig A + -; C3 -; F +; C1q + -
HE 染色： 共查见5个肾小球，显示3个肾小球部分纤维化，一个肾小球完全纤维化。大
部分肾小球系膜区轻－中度增
殖，增殖区系膜细胞2－6个/系膜区， 系膜基质增生扩大，毛细血管壁大致正常，肾小
管上皮细胞肿胀，空泡变性，部
分肾小管管腔扩张，可见少量蛋白管型。肾 |
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l*******6
发帖数: 34 | 30 Preload = ventricular EDV
AV shunt bypasses capillary beds and routes bld directly from arterial to
venous system. Increased preload causes a rightward widening of the PV loop,
which is a increased EDV. Please kindly correct me if I am wrong. Thanks |
|
g*******l
发帖数: 58 | 31 A 42-year-old man consults a physician because he has a "lump" on his
forearm. Examination of the arm demonstrates a 3-cm diameter nodule
protruding above the forearm surface. The lesion is covered with apparently
normal skin and is soft and freely movable. It location appears to be
subcutaneous. The lesion has been slowly growing over the past 2 years, and
the patient has experienced no discomfort. He has consulted a physician at
this time because his wife keeps pestering him to get something d... 阅读全帖 |
|
n*****n
发帖数: 119 | 32 别处看到的，觉得还不错，不排除有些拼写错误~
POMR (Problem-Oriented Medical Records)表格式住院病历
Biographical data:
一般项目：
Name Age Sex Marital status Nativity Race
姓名 年龄 性别 婚否 籍贯 民族
Occupation Date of admission Informant History
职业 入院日期 病史叙述者 病史
Chief complaint
主诉
History of present illness
现病史
Past history
既往史：
Previous health status: well ordinary bad Infectious diseases
平素健康状况： 良好 一般 较差 传染病史
Immunizations Allergies: N Y clinical manifestation
预防接种史 过敏史 无 有 临床表现
allergen: Trauma: Surgery:
过敏原 外伤... 阅读全帖 |
|
e****0
发帖数: 678 | 33 • Compartment syndrome
Severe pain worsened on passive range of motion
Pressure can be measured using a needle and pressure transducing
catheter system.
Pressure > 30 mmHg cessation of blood flow through the
capillaries.
• stress fracture
2nd, 3rd, 4th metatarsal are managed conservatively.
5th metatarsal such as Jones fracture needs surgical
intervention.
• Colonic diverticula are most common in the s... 阅读全帖 |
|
g*******g
发帖数: 196 | 34 谢谢！
From wiki，Mesangial cells is specialized SMC.
Mesangial cells are specialized cells around blood vessels in the kidneys,
at the mesangium. They are specialized smooth muscle cells that function to
regulate blood flow through the capillaries, usually divided into two types,
each having a very distinct function and location
我查了下refs，renal repair is mainly based on kidney epithelial cells.
比如这篇文章
Cell Stem Cell. 2008 Mar 6;2(3):284-91. doi: 10.1016/j.stem.2008.01.014.
Intrinsic epithelial cells... 阅读全帖 |
|
m******e
发帖数: 432 | 35 电解液是通过capillary action进去的，但是有缝隙呀？电解液还是会通过挥发而损失
的呀？针对很多论文的说。如果产业化，我相信还是会用专门的材料密封的。 |
|
m******e
发帖数: 432 | 36 male.发现有时候说话夸张点有利于缓解压力。
我觉得电池的封装有个问题。工作电极是ZnO/FTO, 辅助电极是platinized FTO。中间
放一张spacer，再用heavy-duty clips将他们夹住，然后通过capillary force将电解
液注入。怎么控制ZnO的纳米结构不被破坏？如果不同结构的ZnO在封装的过程中受到的
影响不一样，如何比较结构的优劣呢？我是做合成的，做device不是我的强项。
有谁做过DSSCs？我能跟你电话联系以下吗(我现在在美国)？时间紧迫呀！各位XDJM们
，帮帮忙。如果愿意帮忙的话，可以给我你的电话吗？我觉得这样做有点疯狂，但我现
在文章就差这一组数据，文章都按了半年了。 |
|
y******y
发帖数: 5 | 37 J. Sun, C.J. Tang, P. Zhan, Z.L. Han, Z.S. Cao, and Z.L. Wang “Fabrication
of centimeter-sized single-domain two-dimensional colloidal crystals in a
wedge-shaped cell under capillary forces”, Langmuir 26, 7859 (2010).
我这个方法做的膜很均匀
如果有问题可以发邮件问我。求文章引用，也想认识同行。
s******[email protected] |
|
y******y
发帖数: 5 | 38 J. Sun, C.J. Tang, P. Zhan, Z.L. Han, Z.S. Cao, and Z.L. Wang “Fabrication
of centimeter-sized single-domain two-dimensional colloidal crystals in a
wedge-shaped cell under capillary forces”, Langmuir 26, 7859 (2010).
我这个方法做的膜很均匀
如果有问题可以发邮件问我。求文章引用，也想认识同行。
s******[email protected] |
|
E****e
发帖数: 315 | 39 Bare-silica capillary is only a few bucks if you want to burn the detection
window yourself. It cost about 35$ if you buy directly from beckman, they
already made the detection window for you. |
|
x****o
发帖数: 60 | 40 【 以下文字转载自 JobHunting 讨论区 】
发信人: xiaoao (吃了睡,睡了再吃), 信区: JobHunting
标 题: analytical scientist opportunity (Boston area)
关键字: analytical job,Boston area,biopharma
发信站: BBS 未名空间站 (Sun May 15 14:01:14 2016, 美东)
A long-term contract job opportunity is available in the Boston area. The
person will work for a big biopharmaceutical company through a third party.
The Contract Personnel will have the following minimum requirements:
• BA or BS in Chemistry, Biochemistry, Biology, Microbiology or
e... 阅读全帖 |
|
S*****a
发帖数: 63 | 41 Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
We are seeking an experienced and highly motivated Scientist with strong
hands-on experience in antibody display (phage/yeast) to support discovery
research projects by screening to identify lead candidate therapeutic
antibodies, as well as engineering molecules for improved... 阅读全帖 |
|
j***o
发帖数: 5096 | 42 表面波surface waves回复力就是重力，回复力是表面张力的叫毛细波，capillary
wave，表面波是区别于内波internal waves而言的，回复力都是重力 |
|
|
发帖数: 1 | 44 Phys. Rev. E 93, 066103 (2016) – Published 29 June
2016
In this Comment we point out some shortcomings in two
papers [N. Laskin, Phys. Rev. E 62, 3135 (2000); N.
Laskin, Phys. Rev. E 66, 056108 (2002)]. We prove
that the fractional uncertainty relation does not
hold generally. The probability continuity equation
in fractional quantum mechanics has a missing source
term, which leads to particle teleportation, i.e., a
particle can teleport from a place to another. Since
the relativistic kinetic en... 阅读全帖 |
|
b********l
发帖数: 24 | 45 If you can find these papers, please email me at l*******[email protected]. thanks
Natrajan, V. K. & Christensen, K. T. 2007. A Microscopic Particle Image
Velocimetry Study of Transition to Turbulence in Microscale Capillaries.
37th AIAA Fluid Dynamics Conference, AIAA Paper 2007-3990.
Xu Zheng, Zhan-hua Silber-Li. 2008. Measurement of velocity profiles in a
rectangular microchannel with aspect ratio α = 0.35. Experiments in
Fluids. Don't know about volume number and pages. I couldn't find it
anywhe |
|
A*******s
发帖数: 9638 | 46 昨天晚上睡得晚， 3am医院ICU page我， 我迷迷糊糊打电话， 刚说我不oncall，对方
说了个名字，我一下子全醒了。
这个病人是我的一个老病人， 在医院工作，67岁，她有osler-weber-rendu disease，
epistaxis是常事， 最近出现A-Fib， 因为coumadin引起出血， 所以不能服。
一个星期以后， 她出现中风，因为出血的原因， 不能上heparin， 第二天她在医院又出现右手臂动脉栓塞。 所幸手术后没事。 没办法，她需要抗凝， 所以开始服用Pradaxa。
出院后epistaxis似乎没大问题， 没想到她出现贫血， 血色素只有6。 入院后输血， Pradaxa只能停掉。
(中文太难打了。。。）
I started her on heparin. Consulted GI/Surgery for endoscopy. Surgery did
the upper and lower GI scope and I was told she has possible AVM but no
active bleeding.
I do not kn... 阅读全帖 |
|
s******t
发帖数: 579 | 47 I have been thinking about this kind of dilemmas a lot times and everytime I
felt nervous about it. If I face that kind of situation in the future, I
don't know what should I do.
Is it true that the doctors deal with life threatening situation first then,
thinking about others? Do we have a better approach for this situation? |
|
A*******s
发帖数: 9638 | 48 Risks over benefits.
You are absolutely right. We need to take care of the critical issues first
. In her case, after stroke happened , I had to prevent her from recurrent
stroke first and took the chance of epistaxia.
I believe she had internal bleeding other than epistaxia after being on
Pradaxa.
But just being off heparin several hours for endoscopy, she had another
stroke.
I am helpless.
I
then, |
|
y******a
发帖数: 590 | 49 I will not put her on oral anticoagulation. She has a history of epistaxis,
and GI bleed from AVM, it is possible that she has AVMs in her brain.
anticoagulation may put her at higher risk of ICH, not only GI bleed. |
|
A*******s
发帖数: 9638 | 50 But she was having emboli. 2 events within 2 days! One in the brain and the
other in the arm.
epistaxis, |
|
