z***x
发帖数: 80 | 1 I have no experience of isolating RNA from cartilage but need to do it soon.
Cartilage contains a lot of proteoglycans and glycosaminoglycans (GAGs). I
first homogenized cartilage into powders by freeze-milling. I then tried
guanidine and phenol to dissolve the cartilage powder but it was not quite
efficient. Most of the 100mg of cartilage powder was not dissolved in 2 ml
guanidine/phenol (Qiagen tissue lysis reagent).
The other question is that RNA and GAGs are both negatively charged polymers
... 阅读全帖 |
|
u*******a
发帖数: 6 | 2 共10瓶， AMAZON 付款
~~ Brand New, Manufacturer Sealed, Fasting shipping; Ship from CA ~~~~
Product Description
Sharks are perhaps the healthiest creatures on earth. Having survived
virtually unchanged for 400 million years, they are immune to practically
every disease known to man. Scientists believe it is the shark's skeleton,
composed entirely of cartilage. A gift from the sea. Shark cartilage eases
arthritis agony. An excellent source of calcium and phosphorus and contains
amino acids and a famil |
|
a****x
发帖数: 89 | 3 我妈妈最近基本确诊为肺癌晚期，在国内看到一个宣传资料说”鲨克" 可以防止癌细胞
血管的生成，不知
各位有谁对Shark Cartilage比较了解，它的疗效如何？能否在这边买带回国？ |
|
M**U
发帖数: 2752 | 4 帮老公求
与Surfactant, cartilage, nano particles, interface有关的。 |
|
a***o
发帖数: 104 | 5 Journal: Materials Science and Engineering C (IF:3.088)
1. Keywords: Cartilage reconstruction; injectable scaffolds; in-situ forming
gels; temperature-responsive; xyloglucan; growth factor.
2. Keywords: N-acryloyl-glucosamine; modify; chondrogenesis
3. Keywords: Chondrogenesis; articular chondrocytes; mesenchymal stem cells;
dedifferentiation; surface chemistry; poly(ɛ-caprolactone)
请PM你的英文姓名，单位与邮箱以及发表过的相关文章, 我会向编辑推荐,不过不保证
成功率. |
|
g*2
发帖数: 658 | 6 有没有红肿，热的感觉？ 痛的位置在哪里？ 膝盖的伸，曲， 有没有影响？ 能做半蹲
的动作吗？休息的时候痛吗？膝盖动的时候没有发出响声？
膝盖痛很多原因，可以看看下面的。如果去看医生，也可以先知道一些医学单词
KNEE PAIN CAUSES — There are many conditions that can cause knee pain.
Patellofemoral pain syndrome — This is a group of symptoms most commonly
caused by overuse of the knee. It affects many running athletes, and is more
common in women than men. It causes pain in the front of the knee, and pain
may worsen with squatting, running, prolonged sitting, or when climbing or
descending steps. (See "... 阅读全帖 |
|
g*2
发帖数: 658 | 7 这个要慎入。有问题，查个关键词，看看。
SPECIFIC INJURIES
Hip injuries
Overview and approach — Hip injuries are less common in runners than
injuries to the lower extremity and they can be difficult to diagnose.
Nevertheless, during jogging, the hip joint is subjected to loads up to
eight times body weight and both acute and chronic injuries can occur [55].
In runners, the differential diagnosis of hip pain includes gluteus medius
tendinopathy, piriformis syndrome, stress fracture of the femoral neck,
labral tear, a... 阅读全帖 |
|
H********g
发帖数: 43926 | 8 他那个paper是用一种生物降解材料做个耳朵形状的骨架，然后把牛软骨细胞放到骨架
上长，老鼠提供营养。结果可以长出来一个耳朵形状的软骨结构。这个实验说明这种材
料做的骨架加上软骨细胞可以用来制造软骨组织，拿来做整形用。我看这个paper还是
算靠谱的。比如说要隆鼻子，就不用从关节上砍块软骨，可以取点细胞来做一个鼻梁。
当然这个鼻梁会不会一直长一直长，让人变成一个皮诺曹，那就不一定了。
This study evaluates the feasibility of growing tissue-engineered cartilage
in the shape of a human ear using chondrocytes seeded onto a synthetic
biodegradable polymer fashioned in the shape of a 3-year-old child's auricle
. A polymer template was formed in the shape of a human auricle using a
nonwoven m... 阅读全帖 |
|
T****2
发帖数: 94 | 9 本人30多岁，直偏瘦，平时锻炼也不算多，但是也不是完全不动，以快走，慢跑，踢踢
腿，拉拉身体为主。
最近半年右侧hip和髋处疼，平时不疼，但是跑起步来疼的厉害，根本跑不了，快走20-
30分钟也会疼，我一开始以为是不是跑步的时候扭住了，卡住了，或者踢腿的时候踢很
了，要不就是没有warm up拉伤啥的。一开始也没在意，想就是停止跑步踢腿等，平时
就走走。结果半年多，还是没好好，跑两步右侧的髋骨处里面（就是右腿和身体链接处
）就疼的厉害，而且右腿感觉不得劲，感觉总有东西拽着。
于是就约专科医生看，一开始说怀疑有肌肉或者髋骨连接处撕裂，说做个outpatient的
缝合手术就好了，结果MRI做下来，说我要做个 femoral acetabular osteotomy的大手
术，就是把右腿股头截断，然后把链接的骨头和髋骨重新position,再把右腿钉上去，
说是因为右腿和髋骨链接不好是最终原因。
然后这个老头医生觉得我很可怜，不停的说很抱歉你这么惨，没有其他治疗办法了，我
差点再医生办公室哭下来。我出了医院立刻去做MRI的地方要了我的MRI结果，能否请版
上懂的医生帮我看看，我这个能保守治... 阅读全帖 |
|
i***l
发帖数: 1656 | 10 Collagen /ˈkɒlədʒɨn/ is the main structural protein
of the various connective tissues in animals. As the main component of
connective tissue, it is the most abundant protein in mammals,[3] making up
from 25% to 35% of the whole-body protein content. Collagen, in the form of
elongated fibrils, is mostly found in fibrous tissues such as tendons,
ligaments and skin, and is also abundant in corneas, cartilage, bones, blood
vessels, the gut, and intervertebral discs.
Collage... 阅读全帖 |
|
c******n
发帖数: 5697 | 11 early stage degenerative joint disease does not show up on X-rays
only when cartilage has been worn away, you see osteoarthritic changes on
your knee x ray. so unless you are looking for bone fractures or bone
tumor x ray is uselss
you need to look for the cartilage inflammation present. right after
climbing, nothing changes in the bone except fractures. but joint
cartilages are under severe wear(i.e., the unhappy triad). so the better
scan is MRI of the knee.
山。 |
|
w*******h
发帖数: 25 | 12 Runner's knee (also called chondromalacia patellae) is damage to the smooth
cartilage under the kneecap. It begins with a softening of the cartilage.
With time, the cartilage wears down, roughens, or cracks. This can cause
pain, swelling, or a grinding noise.
Resting the knee by avoiding activities that bother it.
Mediciene to reduce pain and swelling.
Exercise to strengthen the thigh muscles that support the knee. Exercise can
also help prevent pain and future problems.
Avoiding activities that |
|
z******9
发帖数: 1644 | 13 前一个问题不能回答，关于costco那个joint triple strength Glucosamine，我是看
的说明买的：
“
Glucosamine is a basic building block for cartilage, synovial fluid and
other connective tissue. It is needed for healthy structure and function of
joints for optimum mobility.†
Chondroitin Sulfate protects existing cartilage and serves as a building
block for healthy new cartilage.†
”
也就是帮助增加和加强关节之间的软骨层。这就是我买它的原因，也不知道对我有用没
有用，反正先吃吃看。 |
|
s***r
发帖数: 358 | 14 Not at all. I got the rib cartilage injury from doing dips. It's the
cartilage that connects my first rib (on the right side) to my sternum, very
close to my collar bone. In the early stage, it would hurt to pick
something up from the floor. Flexing my pecs, shrug my shoulders or
extending my arm backwards would hurt too. But it's got much better now.
As for the scm injury, I still don't know how I got it. This along with the
cartilage injury basically prevents from doing deadlift and any other ... 阅读全帖 |
|
U******u
发帖数: 5829 | 15 I don't believe your cartilage worn down, but most likely joing assistant
ligments injuries, which I do have more sertious than you as I played v-ball
and injured too bad and too oftern before.......
u r still too young to concern cartilage worn down at this moment, no matter
you are 4.5 or 5.0, based on your training/play intensity, far away
comparing to pro.......
cartilage
ankle |
|
s***m
发帖数: 6197 | 16 1. Borjesson, D. L. & Peroni, J. F. (2011). The regenerative medicine
laboratory: facilitating stem cell therapy for equine disease. Clinics in
laboratory medicine 31, 109-23.
2. Chu, C. R., Szczodry, M. & Bruno, S. (2010). Animal models for
cartilage regeneration and repair. Tissue engineering. Part B, Reviews 16,
105-15.
3. Frisbie, D. D. & Stewart, M. C. (2011). Cell-based therapies for
equine joint disease. The Veterinary clinics of North America. Equine
practice 27, 335-49.
4. M... 阅读全帖 |
|
c*********r
发帖数: 1312 | 17 多谢啦！Protocol奉上。
我们是用小鼠胚胎的软骨和骨头染色的protocol。染软骨的话只用Alcian Blue即可。
最后胚胎用BABB处理就会变得透明。BABB危险，而且会腐蚀显微镜，使用时要小心。很
多动物的胚胎都可以用类似的方法染骨头（红色）和软骨（蓝色）：鼠，龟，蛇，鸡，
鱼，蜥蜴，变色龙，蝙蝠。。。而且都很漂亮！
比如这个人，做了好多不同动物的染色还到处办展览：
http://www.shinsekai-th.com/en/photo.php
Protocol：
1. Skeletal Preparations- Cartilage and Bone staining
1.1 Cartilage staining in mouse embryos from E12.5 - E13.5
Dissect out embryos, rinse in 1 x PBS, and place in ice-cold 95% Ethanol for
1 hour.
Change to fresh 95% ETOH and rock overnight at room temper... 阅读全帖 |
|
z****n
发帖数: 5870 | 18 还不到40岁。。。。北方人，从小就住在阴冷阴冷的背阴面，一直有关节炎，以前打球的时候保护措施也做得不太好，最近几年走路的时候两腿膝关
节都有声音。平时如果活动多了，就会膝盖不能伸直或者不能弯曲。但是通常，休息休
息就好了。前几天右膝盖忽然不能伸直了，并且到了比较严重的程度，连续几天都不好
。这才去看医生。照了X-ray 和 MRI.以下 是MRI的分析结果。
骨科大夫建议右膝盖手术，他说手术中会：
1）清理骨头表面，让它更光滑
2）清理关节腔内的各种碎片。。。
不知道是不是真的应该动手术。看病折腾了这么几天，现在不知不觉中，右腿又可以伸
直或者弯曲了,只是还不能快走和跑。现在很纠结，不知道是不是应该动这个手术。。
。。。
Small joint effusion is present. Prominent patellofemoral osteophytes are
noted. There is moderate patellofemoral cartilaginous thinning, with areas
of chondral fibrillation and fissur... 阅读全帖 |
|
J******h
发帖数: 6102 | 19 thanks for your response, I have your point.
So far I will try to strength left leg muscle first, of course with
glucosamine bills. If it doesn't work I will consider further test
including suggested MRI.
A question: from the X-ray, gaps between bones and cap are evenly
distanced, and bones have smooth surfaces interfacing cartilages.
Does this mean that the cartilages are not wearing or tearing out?
changes on
bone
after
better |
|
W********t
发帖数: 8514 | 20 今天去看了专科医生， 一颗心放到了肚子里也凉了一半。这种articular cartilage损
伤和磨损就是一个自然过程，没有什么好担心的， 实在疼的话， 就去打激素， 最后
就是关节置换。 幸好我是轻微的不舒服，不需要担心。医生说了， 高大球打球， 该
玩就玩。
还有市面上这些保护关节的药没有用！ 它们有一些消炎作用， 但并不治疗articular
cartilage。
趁着还能打， enjoy it! |
|
s**a
发帖数: 1995 | 21 我五月觉得膝盖后面疼，去看医生orthopedic surgeon, x光后说有磨损，疼痛是关节
液泄漏，压迫造成的。
医生让停止骑车，去做了6周物理治疗，有改善，但有时候还是疼。
本周做了个MRI,没找出的毛病部分就不写了，毛病部分如下：
There is multifocal thinking of the patellofemoral articular cartilage with
mild subchondral edema in the torcula region of the distal femur. There are
mild patellar osteophytes. The articular cartilage in the lateral
femorotibial compartment is intact.
There is no stress fracture or osteonecrosis, there is a small Baker's cyst.
医生告诉我以后不要骑自行车了，我42岁。当然，偶尔周末玩一玩可以，每天通勤就不
要搞了。
版上学... 阅读全帖 |
|
s*********r
发帖数: 729 | 22 最近几周每周跑30迈, 但是从上一周开始，如果连续两天每天跑超过7迈，右膝盖就会
有些隐隐作痛。虽然并不强烈，但还是有些担心。还有就是如果歇一天不跑就好了。
我Google发现有很多关于"Runner's knee"的资料。下面转载一篇：
How To Improve Conditon Of Runners Knee
By: maggie eliot
Break Studios Contributing Writer
If you suffer from runner's knee, learn about how to improve the condition
of runners knee. Runner's knee ( or Patellofemoral pain) is a common injury
in runners. Runner's knee is characterized by swelling and pain in the
cartilage surrounding the knee cap and the top of the femur. In ru... 阅读全帖 |
|
g***1
发帖数: 515 | 23 There has not been large-scale clinic research on this issue. However, most
physicians recommend Glucosamine as dietary supplement for better joint
health. My experience is that it helps quite a bit, especially after weight
training. We are not young anymore and need help repairing cartilage.
From Mayo Clinic info:
http://www.mayoclinic.com/health/glucosamine/NS_patient-glucosa
"Available evidence from randomized controlled trials supports the use of
glucosamine sulfate in the treatment of osteo... 阅读全帖 |
|
b*********s
发帖数: 6757 | 24 what about the cartilage in the ankle? doc said I don't have much cartilage
left (due to the childhood injury). He said I probably need a ankle
replacement down the road... any recommendation on how to prolong the ankle
while still play tennis?
Thanks!
too |
|
U******u
发帖数: 5829 | 25 but for us old guns, the key is the cartilage which cover joint bone was
worn too badly la.....nothing to do with ligment and muscle. if u compare
with me, my leg muscle might be much thicker and strong than yours, but my
knee cartilage might only half left comparing to you youg ppl. |
|
v*****r
发帖数: 2325 | 26 关节酸是joint ligament tear and cartilage wear?
as the saying epxress: "no pain no gain" .. hope the pain is not serious...
*slight muscle strain, rest and can recover within 3 days.
Allow 10 days to three weeks for mild strains, and six to eight weeks for more severe strains.
*rest 3 days, soreness at tendon/ligament location, could be tendon or ligament micro tear, need rest 4 weeks and more
*chronic soreness, can be cartilage wear, common in running and jumping, not common from weight lifting |
|
l*****o
发帖数: 26631 | 27 wiki上的说法是：
Oral glucosamine is commonly used for the treatment of osteoarthritis. Since
glucosamine is a precursor for glycosaminoglycans, and glycosaminoglycans
are a major component of joint cartilage, supplemental glucosamine may help
to prevent cartilage degeneration and treat arthritis. Its use as a therapy
for osteoarthritis appears safe, but there is conflicting evidence as to its
effectiveness. A Cochrane 2005 meta-analysis of glucosamine for
osteoarthritis found that only "Rotta" prepar |
|
d*b
发帖数: 21830 | 28 不是了，小时候的骨头比老时候多，很多骨头间是Cartilage，等年纪大了Cartilage就
钙化了，这个过程叫bone fusion.
随便举个bone fusion的例子，几个月的小孩可以躺床上咂自己的脚指头，你一大人咂
个脚趾头估计可以创世界记录了。 |
|
w********h
发帖数: 12367 | 29 看文章不能老盯杂志的影响因子，
这三篇文章都超过100次，还不错啦。
Title: Comparative study of the use of poly(glycolic acid), calcium alginate
and pluronics in the engineering of autologous porcine cartilage
Author(s): Cao YL, Rodriguez A, Vacanti M, et al.
Source: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION Volume: 9 Issue:
5 Pages: 475-487 Published: 1998
Times Cited: 149
Title: Tissue engineered neocartilage using plasma derived polymer
substrates and chondrocytes
Author(s): Sims CD, Butler PEM, Cao YL, et al... 阅读全帖 |
|
a*i
发帖数: 1652 | 30 http://247wallst.com/2011/07/18/the-eleven-most-implanted-medic
11. Implantable Cardioverter Defibrillators
> Number of procedures: 133,262
> Total annual expenditure: $5.5 billion
> Average cost per procedure: $40,000
> Major manufacturer: Medtronic (40%), St. Jude Medical, and Boston
Scientific
Cardiac arrhythmia, or improper electric signaling in the heart, occurs in
millions of people a year. While the vast majority are benign, a select few
— usually in patients with a history of heart attac... 阅读全帖 |
|
h******2
发帖数: 1498 | 31 Just talked to doc, he prescribed 3x 600mg of IB /day. Triple flex might
not be effective.
Here is the info:
Patellofemoral pain syndrome is pain in the front of the knee caused by
overuse, weak thigh muscles (quadriceps), or a problem with the way the
kneecap moves. Extra weight may also cause this syndrome. The patella is the
kneecap, and the femur is the thighbone. Some people may have pain in the
front of the knee from a condition called chondromalacia, in which the
underside of the knee ca... 阅读全帖 |
|
p***o
发帖数: 714 | 32 by pennbrook@168
好久没有正经写点东西了, 今天说说rheumatoid arthritis.
Inappropriate inflammatory cell response is associated with autoimmune
diseases, a spectrum of disorders in which the immune system malfunctions
and causes the body to attack its own organs, tissues, and cells.
The body's own immune system attacks a joint's synovial membrane, which
becomes inflamed, secretes fluid, and the cartilage becomes rough and pitted.
Rheumatoid arthritis大家应该都知道, 基本上每个药出来都是blockbuster.
现在的几种主要治疗方法是:
1. NSAID: 象Pf... 阅读全帖 |
|
r***l
发帖数: 298 | 33 it can be ligament/tendon tear or cartilage/meiscus tear.
you need a MRI. X-ray has no soft-tissue contrast and is rendered useless.
Ultrasound may be revealing, in the hands of an experienced sonographer.
conventional MRI (aka, no contrast agent) should be covered pretty well by
most insurance carriers.
Unless you have severe cartilage tearing, as with some unscrupulous marathon
runners, you should not need contrast.
You may need a referral from your family doctor b4 you can see a knee
speciali |
|
xt
发帖数: 17532 | 34 嗯，chicken cartilage string.可以翻译成鸡肉软骨字符串?
上海的东西向来是份特别小而且贵 |
|
d*b
发帖数: 21830 | 35 你太搞笑了，江青同志都从上海走到延安了，还比不上daling的2根Cartilage？ |
|
发帖数: 1 | 36 Nexious
3 points
·
1 year ago
he did a good job of making a semi-neutral film.
How much research into the case have you done outside of the documentary
itself to verify that it is as neutral as you contend? Do you disagree with
all of the "points left out" lists that others have made about it?
Kathleen Peterson worked for a dot com bubble-affected company that was
figuratively burning down ($398b to $5b in 2 yrs). Almost all workers had
been laid off and she confided in a friend that she worried... 阅读全帖 |
|
发帖数: 1 | 37 Sexually transmitted diseases reduce the willingness of female baboons to
mate
8 hours ago
The German Primate Center
Would you like to receive trending story notifications on your smartphone?
A female olive baboon at peak estrus rejects the mating attempt of a male.
Credit: Filipa Paciência
Sexually transmitted diseases are widespread among animals and humans.
Humans, however, know a multitude of protective and hygienic measures to
protect themselves from infection. An international research... 阅读全帖 |
|
l****z
发帖数: 29846 | 38 By JENNIFER CORBETT DOOREN
The Food and Drug Administration said Wednesday it found 14 additional
Chinese companies supplied contaminated raw material to make heparin, a
widely used blood thinner.
The companies supplied the materials in 2008, when the FDA found a link
between contaminated heparin marketed by Baxter International Inc. to some
Chinese suppliers of the active ingredient used in heparin. The
contamination was linked to 80 deaths in U.S. patients and hundreds of
allergic reactions. T... 阅读全帖 |
|
l***d
发帖数: 1798 | 39 FOR IMMEDIATE RELEASE
National Academy of Engineering Elects 66 Members and 10 Foreign Associates
WASHINGTON — The National Academy of Engineering (NAE) has elected 66 new m
embers and 10 foreign associates, announced NAE President Charles M. Vest to
day. This brings the total U.S. membership to 2,254 and the number of forei
gn associates to 206.
Election to the National Academy of Engineering is among the highest profess
ional distinctions accorded to an engineer. Academy membership honors th... 阅读全帖 |
|
|
|
c**i
发帖数: 6973 | 42 Barbecue and American Culture l Fire in the hole; Barbecue navigates the
twin perils of mass appeal and nostalgia. Economist, Dec 16, 2010.
http://www.economist.com/node
/17722664?story_id=17722664
My comment:
(a) The takeaway is
"American barbecue falls into four broad geographic categories * * * [a] In
North Carolina pork, either whole hog or shoulder, is seasoned minimally if
at all. The sauce, applied at table, varies. In the eastern part of the
state it is usually nothing more than cider v... 阅读全帖 |
|
t***a
发帖数: 45 | 43 Regeneron is a fully integrated biopharmaceutical company that discovers,
develops, and commercializes
medicines for the treatment of serious medical conditions. In addition to
ARCALYST (rilonacept) Injection for
Subcutaneous Use, its first commercialized product, Regeneron has
therapeutic candidates in Phase 3 clinical
trials for the potential treatment of gout, diseases of the eye (wet age-
related macular degeneration and
central retinal vein occlusion), and certain cancers. Additional therap... 阅读全帖 |
|
t***a
发帖数: 45 | 44 Regeneron (in NY)is a fully integrated biopharmaceutical company that
discovers, develops, and
commercializes medicines for the treatment of serious medical conditions. In
addition to ARCALYST
(rilonacept) Injection for Subcutaneous Use, its first commercialized
product, Regeneron has therapeutic
candidates in Phase 3 clinical trials for the potential treatment of gout,
diseases of the eye (wet age-related
macular degeneration and central retinal vein occlusion), and certain
cancers. Additional ... 阅读全帖 |
|
n*********r
发帖数: 18 | 45 鲨鱼软骨对风湿性关节炎确实有效,我的三姨服用过,效果相当明显.用的牌子是Uncle
Bill 的Shark cartilage. |
|
u*****d
发帖数: 1009 | 46 I don't know much about shark cartilage, but there's certainly a lot of
herbal products that mess around with the INR from coumadin/warfarin.
I'd stay away from those if I were the patient. It's hard enough to finally
get an INR stable, and it's not worth it to make it fluctuating again.
If osteoarthritis has become serious, then knee replacement might be an
option. |
|
l*******n
发帖数: 68 | 47 It depends on the problems. For minor issues of joint and cartilage,
internist can take care of it. For more complicated problems, you should see
an orthopedic surgeon. |
|
l*******n
发帖数: 68 | 48 Gout (metabolic arthritis) is a disease created by a buildup of uric acid.
In this condition, crystals of monosodium urate or uric acid are deposited
on the articular cartilage of joints, tendons and surrounding tissues. These
crystals cause inflammation and pain, both severe. If untreated, the
crystals form tophi, which can cause significant tissue damage. Gout results
from a combination of elevated concentrations of uric acid and overall
acidity in the bloodstream.
Treatment has three object |
|
t*******n
发帖数: 4445 | 49 Osteoarthritis.
Glucosamine/chondroitin sulfate supplement is said to be somewhat helpful.
You can get them at health food stores.
She should also consider taking NSAIDs for pain and inflammation, as well as
get physical therapy to strength the muscle and reduce cartilage damage. |
|
t*n
发帖数: 14458 | 50 关节pain没了
胃pain开始
Osteoarthritis.
Glucosamine/chondroitin sulfate supplement is said to be somewhat helpful.
You can get them at health food stores.
She should also consider taking NSAIDs for pain and inflammation, as well as
get physical therapy to strength the muscle and reduce cartilage damage. |
|
