a********e
发帖数: 3771 | 1 最近一次做western，用NEPER kit提取胞浆里的ERK和p38蛋白。因为用划伤组织做刺激
，脱落不少细胞，我就把medium离心得到这些脱落的细胞。同时在培养皿里加冰PBS，
在冰PBS里刮下剩余的细胞放进脱落的细胞里再次离心。然后用kit里CER lysis buffer
裂解（已加入inhibitor cocktail）。
裂解的时候我vortex两次，每次15sec。NEPER protocol要求vortext一次，15sec即可。
结果照片洗出来什么都没有，连EGF刺激的positive control都没出来。
以前只用过一次NEPER，erk出来band，而且结果可信。不同点在于没有收集脱落的细胞。另外，这次实验煮沸变性的时间长了，用了15min。
所以我考虑是不是多次离心、vortex是蛋白降解，也可能是收集脱落细胞的时候medium
和细胞都没有冷却到ice cold，磷酸酶还在起作用。煮沸时间长我不确定会不会对实验有影响。
另外，p38一直没做出来过。用超声裂解细胞也好，用NEPER vortex裂解也好都没出过
band。
请高手指点，同行探讨。谢谢。 |
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p*****m
发帖数: 7030 | 2 恩纳 你这个说的蛮有意思 啥时候有了toxicity marker的idea出来八八
我觉得这个主要还是delivery的进步，不管是用病毒的壳还是用NP+某targeting prote
in。这些肯定是会很有作用的（对了 我看过些用HBV做的壳用来delivery的文章 似乎实
际用起来效果没那么好？） 但是按说这个target specificity没有完全解决PKPD的问题
，比如说吧 这样也阻止不了most if not all的NP进到肝细胞里面，然后被无穷无尽的
P450和其他酶搞成碎片或者变个模样；
同样的，如果要是简单的杀死细胞，NP里面弄个toxin也还好，但是大部分的病除了can
cer以外你未必想要杀死那么多细胞（还是你这个肝细胞的例子 其实HBV里面致病的不是
HBV倒是肝细胞死掉，你要是也一杀了之那就等于催化肝病了），二是要定点清除细胞里
的感染性物质（对感染性疾病而言） 或者改变细胞内某个蛋白的活性等等（对代谢性疾
病而言），这样你还要保证你用的小分子在实施作用之前不会在lysosome或者golgi ap
peratus里面被加几个修饰集团 等等，或者干 |
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j****e
发帖数: 35 | 3 generally schools require a bachelor degree for graduate admission. som
e schools, however, might not know the difference between graduation cer
tificate and diploma from China. i guess it's case by case depending on
your luck. |
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w*g
发帖数: 413 | 4 “嫒ㄉ
本帖版权归提交者二一老人和在线利方中文网站共同所有，
其它网站转载或转贴须注明出处，传统媒体转载须事先与SRSNET联系
※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※
知识经济是个筐？
第366篇98年9月12日收存于经济参考文摘：http://cer.yeah.net
※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※※
知识经济是个筐？
□桂勇 周春雨
“知识经济”在近两年中已被炒到居高不下的地步。现在冷
眼看来，至少可以提出以下三个疑问。知识经济是个筐？
时下关于知识经济的探讨存在着一个带着普遍性的问题，那
就是概念本身含糊不清，所作探讨多为浅层次、表面性的，不免
流于空泛、随意，有时还有相互矛盾之嫌。自从被媒体大肆炒作
之后，一时之间街头巷尾处处可闻“知识经济”之名，人人都大
讲特讲知识经济，并将之 |
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z****e
发帖数: 438 | 5 看看文章的received date, revised date 和accepted date. 有点儿太不严肃了吧。
CER其他文章都这么迅速么？ |
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L******k
发帖数: 33825 | 6 secondary Education 在ny要有master！！
本科毕业 给你5年时间 拿到 master！！
我们program 拿的是 initial 的 certificate ， 以后才成换成 professional 的
cer
tificate！！
现在 政府经费 cut 很厉害 学费又涨， 我们program 开始不招生了， nyu的 master
不知
道 还继续招生吗！！ |
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d****o
发帖数: 1112 | 7 hoho, I figured that out from your ID, sorry about that.
I have designed a computer server like box before. Then we hired a firm to
talk to UL to get our product pass UL testing. We also pass EMC testing as
well so we can sell them now. I guess we paid about 20k get our product cer
tified by UL and EMC. It is damn expensive.
Now I am trying to design a small instrument and then sell to US market on m
y own. Not sure if I need to go thru same UL/EMC process to get certified a
nd how much I h |
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g******u
发帖数: 3060 | 8 you can do EMC pretty much at no charge. Although it won't be certified but
you can prove to the customer.
There is a trick, talk to a filter manufacturer and they might test free for
you.
you can do UL after you sold some.
to
as
cer
m
a |
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K**********n
发帖数: 1197 | 9 学术界珍稀有限的原版外文资料，
Remote Mind Control Technology
Reprinted from SECRET AND SUPPRESSED: BANNED IDEAS AND HIDDEN
HISTORY, edited by Jim Keith, $12.95, available from
1-800-680-INET.
There had been an ongoing controversy over health effects of electromagnetic
fields (EMF) for years (e.g., extremely low frequency radiation and the
Navy's Project Seafarer; emissions of high power lines and video display
terminals; radar and other military and industrial sources of radio
frequencies and micr... 阅读全帖 |
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k****a
发帖数: 16 | 10 There is no abuse involved here. What I am claiming is that the court should
first consider whether the parents have abandoned or done anything with the
child. Then the court can consider the best interest of the child, i.e. who
should the child live with.
I have followed this case for a while. The first judge excuded himself for
possible bias after dragging this case longer than the statutory limit of 2
years.
I may not know the family law that much since I have never worked in that
area. I cer |
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s*******f
发帖数: 109 | 11 Although the proposed class lawsuit will fall down when it is filed, it is
good to see that Chinese community is showing its effort in fighting for
their interest in an organized manner. I just have some comments on the
class lawsuit itself.
First, for the class lawsuit to stand, the plaintiffs must have standing. I
mentioned this issue in the other note. I do not want to repeat here.
Second, even assuming that the plaintiffs (class members) have the standing,
plaintiffs must obtain an court cer |
|
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k**e
发帖数: 2728 | 13 Based on the information I know, graduated before 1999 is okay (or entered m
ed school in Preventive Medicine before 1999?) to take the exam. I am not su
re which version is correct. As far as I know, ECFMG makes clear cut for cer
tain years limitation. That's why I recommened Swing2 to give ECFMG a call t
o find it out. They made this policy after communicating with the Bureau of
Education in P.R. China. It used to have some grey area years ago with regar
d to this major, but it seems no longer |
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a********e
发帖数: 3771 | 14 【 以下文字转载自 Biology 讨论区 】
发信人: andyzzdlee (麦地之一看二慢三通过), 信区: Biology
标 题: western提蛋白的困惑：多次离心vortex会不会降解蛋白
发信站: BBS 未名空间站 (Wed Jan 20 23:38:17 2010, 美东)
最近一次做western，用NEPER kit提取胞浆里的ERK和p38蛋白。因为用划伤组织做刺激
，脱落不少细胞，我就把medium离心得到这些脱落的细胞。同时在培养皿里加冰PBS，
在冰PBS里刮下剩余的细胞放进脱落的细胞里再次离心。然后用kit里CER lysis buffer
裂解（已加入inhibitor cocktail）。
裂解的时候我vortex两次，每次15sec。NEPER protocol要求vortext一次，15sec即可。
结果照片洗出来什么都没有，连EGF刺激的positive control都没出来。
以前只用过一次NEPER，erk出来band，而且结果可信。不同点在于没有收集脱落的细胞。
所以我考虑是不是多次离心、vortex是蛋白降解，也可能是收集脱落细 |
|
f**********r
发帖数: 95 | 15 【 以下文字转载自 Medicine 讨论区 】
发信人: flyingbutter (flyingbuffer是我的马甲), 信区: Medicine
标 题: 帮妈妈的朋友问的，请熟悉Rheumatology的牛人帮忙看看，非常感谢
发信站: BBS 未名空间站 (Sat Jun 29 15:39:08 2013, 美东)
我有病人自述的病史 （在二楼）和全部的labs,不过很长，我试着写了个摘要在下面，
如果您需要更多的信息而且有时间，可以看看病人自己写的病史，我还可以把lab
tests发给您。
病人女，49岁。
家族史
母亲：2008年元月因突然双臂疼痛，由医院诊断为类风湿性关节炎，当时用激素治疗了
一个月，其它的药吃到当年的8月份停的，到当年的10月份基本治愈；之后一直自我感
觉良好，未在检查、治疗。直至2013年初，因发热去医院检查发现肺部感染并间质性改
变，白细胞、血小板减少，作进一步检查最后确诊为红斑狼疮，现服用美卓乐及羟氯喹。
弟弟：十六岁左右确诊罹患牛皮癣。
侄子：今年21岁，日前确诊罹患牛皮癣。
病史
1995年的4、5月份患带状疱疹（Herpes zoste... 阅读全帖 |
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m*******r
发帖数: 481 | 16 In a very top MatSE department, a professor in eletroceramics is very famous
. But when you see his publication list, you will very rarely see papers
beyond J. Am. Cer. Soc or Phys. Rev. B. But his repuation is too high to
imagine. |
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w***g
发帖数: 712 | 17 The GoaL of Standardization
A group at the NHGRI, headed by imaging scien- tist Yidong Chen, is developin
g software that will help to interpret the huge trove of data that microarray
s yield. "We're building a Vind of fin- gerprint record against which to comp
are new array data," explains Chen. "We're at a very, very preliminary stage,
seeing if we can derive any- thing from the'fingerprints' of specific cancers
. At the moment, we're concentrating on breast can cer, prostate cancer, and
melan |
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g*g
发帖数: 4164 | 18 【 以下文字转载自 PDA 讨论区 】
发信人: miclxx (听雨), 信区: PDA
标 题: 分享一下我的5800上安装的软件
发信站: BBS 未名空间站 (Fri Jan 1 15:05:33 2010, 美东)
5800入手两个星期，现在基本上折腾的差不多了。自我感觉性价比相当高。当然这取决
于你的使用习惯。当初受版上很多帖子帮助良多，这里就说一下自己的心得，作为回馈。
尽管有人说symbian系统的软件不如ppc多，其实足够了。软件我觉得有3到4个你能经常
用的就足够，装多了总会有系统不稳定的隐患。
我是在dell买的5800的nav版本，没有data plan，没有hack过，也没有刷中文。不过如
果你想的话，dospy.com上都可以找到教程。
1.证书和签名：
很多软件要自签名后才能安装。这是我觉得symbian系统最土的部分，实在不方便。手
机到手后，找到你的IEMI号码，到这里提交http://cer.dospy.com/，系统只在每天11点（北京时间）后开放，提交后，24小时内可以来查看（重新输入号码后提交即为查看）。如果证书做好了，就可以下载了。下载这 |
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y*****l
发帖数: 5997 | 19 The 12th Annual BIO CEO & Investor Conference
An International Biotechnology Investor Conference Featuring Established &
Emerging Public Companies
February 8-9, 2010
Waldorf-Astoria
New York City
The BIO CEO & Investor Conference is the largest investor conference focused
on publicly traded biotech companies. Because our mission is to support
industry-wide success, we present a broad and unbiased view of investment
opportunities.
Our therapeutic workshops feature the latest information on pipeli... 阅读全帖 |
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t*******o
发帖数: 1464 | 20 刚才看了一下C字头的，发现中概被delete的远多于add的。
Add CALIPER LIFE SCIENCES CALP United States Health Care
Add CALIX INC CALX United States Technology
Delete CALLIDUS SOFTWARE INC CALD United States Technology
Add CALLON PETROLEUM CO CPE United States Energy
Add CAMAC ENERGY INC CAK United States Energy
Add CAMBIUM LEARNING GRP INC ABCD United States Consumer
Discretionary
Delete CAPE BANCORP INC CBNJ United S... 阅读全帖 |
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b*****h
发帖数: 783 | 21 来自主题: _pennystock版 - hogs 在hogs提供零售店地址后，CER的调查人员在上海地区做进一步调查，在上海的104家，
走访了78家，证实了其中的35家。
后来在hogs的协助下，他们一起去抽样调查了10家，结果是找到4家，3家没找到，3家
地址不对。双方的态度都比较强硬。
目前看来hogs 的retail store 比原来报告推测的要多，但是hogs对retail store管理
比较混乱，数据有问题。 |
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b*z
发帖数: 48 | 23
no. we don't do complementation test on human beings, but on
a number of experimental organisms, including the famous
fruit fly. it would have been inhuman to cross a man and a woman.
suprisingly enough, almost all organism use the same
biochemical machinery to generate energy and so on. therefore,
from the studies of those organism we can to a great extent
learn our own species. of course, not all information about
out genes are discovered in this way.
some genes are found when patients of cer |
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