d****7
发帖数: 109 | 1 很正常，因为ChIPed DNA浓度极低，Nanodrop都不准的，我从来都是只用Nanodrop测
Input DNA，从来不测ChIPed DNA
Nanodrop测得是OD吸收值，用phenol的话，会有一些phenol或者没取干净的蛋白质类的
也会吸光，影响浓度。
对于ChIPed DNA，我们这的core facillity用Q-bit，Q-bit更准，因为据说是根据与
DNA特异性结合的原理而测的，具体是啥我忘了。
我这有个例子你可以参考，上个月我的数据，
测ChIPed DNA浓度：nanodrop：15.3ng/ul, Q-bit: 0.236ng/ul
对于看不见沉淀，你加carrier了么？glycogen or yeast tRNA之类的。 |
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b****g
发帖数: 7311 | 2 yes, chiping is the hardest in the 3 guys, leland, and yu lu have more
weakpoints.
Chiping is middlechool team member, has more solid foundation. |
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c****1
发帖数: 5654 | 3
i feel Chiping's style is like hpzd's, defence is better than their
offense...
but leland can beat Chiping most cases. |
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b****g
发帖数: 7311 | 4 yes, chiping is the hardest in the 3 guys, leland, and yu lu have more
weakpoints.
Chiping is middlechool team member, has more solid foundation. |
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c****1
发帖数: 5654 | 5
i feel Chiping's style is like hpzd's, defence is better than their
offense...
but leland can beat Chiping most cases. |
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F*****e
发帖数: 182 | 6 多谢momoshuihan。
我们是ChIP新手，希望大牛们指导。问题是：
1.input DNA
2.IgG-ChIPed DNA
3.Sample anitbody-ChIPed DNA
这三种样品是否都需要做sequencing？DNA是否得到enrichment是和1比较还是2比较？
另外能介绍一下怎样做qulity control analysis么？多谢！！ |
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w*****a
发帖数: 437 | 7 是，我用phenol提出来的浓度好多了，不过为什么IgG ChIPed DNA 比我用Antibody
ChIPed DNA 要高，感觉不妙。
第一次做用q-PCR validate了，work的，这次是重复。 |
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c****h
发帖数: 4968 | 8 连智利都用全电公交了。订了200辆，交付100辆。要是用欧美品牌，肯定买不了这么多
。这些发展中国家和中等收入国家将是中国将来巨大的市场。
https://www.youtube.com/watch?v=Y5zGISCVPIQ
Wilder Manolo Flores Contreras1 week ago (edited)
Greetings to Chileans from Peru for their great bus fleet.
Reply 42
alexis1 week ago
They should enact an effective prison penalty law for anyone who attacks
these buses
bokys casal2 weeks ago
Good for electromobility! Better throw them straight to the regions and
provinces, there will be more secure, now in Santia... 阅读全帖 |
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W*******d
发帖数: 446 | 9 There are fairly amount of torque steer for both (chiped GTI and MS3). You
have to regulate your foot on gas, but that's the way FWD suppose to be
driven anyway. You are not getting much if you floor gas pedal.
RWD car also have torque steer problem,but it's on the tail. Try to floor
high power car like Corvette, the tail will want to come out in either
direction randomly. pretty scary too. |
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j***e
发帖数: 708 | 10 my understanding is:
$5 is fine if everybody chiped in for holiday cheer in public schools.
preschool teachers need to be tipped a little more, usually greater than $10
per kid if organized.
and more for daycare care-givers around $25. |
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v***s
发帖数: 4031 | 11 Sounds like a good plan. LOL.
so the path to break 100 looks simple
1)limit damage from driver
2)pictching and chiping within 20ft, not a high goal. no chip/pitch over or
short of green.
3)putting lower to lower end of 2-3. first 2.3 ba... |
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v*****r
发帖数: 2325 | 12 you must be very good at putting/chiping/pitching. no wonder your slogan is "up and down".
any tips for lag long putts close to pin?
carpet good for practice putt line, and some distance control.
i don't expect average <2putts in near term.
depends how tough green is, if the green is slopy, low 2.x is not easy.
also depends on your approach shot. if approach from 100+ yard away, you
expect 20-30-40 feet to pin. with slopy green, 2putts is not a ganrantee...
if no GIR, but you get to pitch or |
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k****e
发帖数: 2758 | 13 he plays like an armature |
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x****5
发帖数: 1564 | 15 打了一辈子高尔夫的老虎竟然也会一场打这么多蛙跳，不可思议。
我打球时出现切球蛙跳最懊恼，非常影响情绪。可以想见老虎多郁闷了。 |
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l****e
发帖数: 276 | 16 今天又打了一个，好像没练的样子
[在 killme (killme) 的大作中提到：]
：he plays like an armature |
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ET
发帖数: 10701 | 17 对与他球的所在的位置，我一般只有一个念头，把球放在果岭上。
有些位置让球挺得非常靠近球洞近乎不是我们业余10+选手该考虑的事。 |
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C******s
发帖数: 1917 | 18 同意。
用最大保险度的打法把球停在果岭上是业余选手的唯一目标。
能推，就推。
不能推能打低抛物线，就打低滚。
最后的选择是高抛。 |
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u******m
发帖数: 307 | 19 I always putt from that kind of lie. Made up and down more than half the
time. |
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h**d
发帖数: 5161 | 20 还可以。输了Chiping，但是赢了两个苦手Lu和Mike（正胶加长胶）。老赵回国了，很
想再试试他的长胶。 |
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b****g
发帖数: 7311 | 23 他发挥太失常，好几天没打。
他和leland, yu lu, chiping差不多，上次和他们三打的，好象还赢得多些。
james n, 反手不敢拉的话，实力下降不少。 |
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S*********r
发帖数: 4729 | 24 输了。
我们的一号老削球手，50-60岁了，体力超好，连打6场，灭了chiping, wallwall， 里
程碑，等各路高手，和 Jim Chai 2：2握手言和，保持不败金身。 |
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l***a
发帖数: 12410 | 25 那基本上就可以理解intel和crucial同一代的产品基本chiping，first tier？intel除
了x25-m没有别的系列产品了？
reliability到底指啥？难道ssd也会有类似硬盘那种划坏盘面损坏盘道的问题？ |
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n********k
发帖数: 2818 | 26 Sure, I would agree from statistics, however, Since the gene as you know
have very conserved binding site and have been ChIPed so many time----what
would be ur thoughts on the fact: most of the peaks (1200 for one condition
and 800 for a 2nd---the numbers also agree with my expectation between the
two conditions) are confirmed targets while the rest could be potentials
ones... |
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m*********n
发帖数: 215 | 27 what....I mean, you do not even know what DNA sequence you want to be "
riched", how do you know they are "riched" without sequencing? Are you just
wanna generally look at what this TF binds in the whole genome, or you are
testing whether it binds to a specific gene?
Ok, 先做library来获得更多的DNA.
Then... do you know the consensus binding sequence of this TF?
If not, and given that you do not have a positive control, you really need
to send the library for deep sequencing, and compare to control IgG-Ch... 阅读全帖 |
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m*********n
发帖数: 215 | 28 You can just use input DNA and Antibody-ChIPed DNA for sequencing. For
quality control, your sequencing core facility will run the test for you, e.
g. Bio-analyzer chip etc.
One thing just hit me... at least in our hand, the DNA fragments needs to be
smaller for sequencing than for qPCR... I assume that you checked your
sonication efficiency and know the size of your smears before you did ChIP..
. Again, the best way is to talk to your sequencing facility and ask them
for technical details. This... 阅读全帖 |
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