T**7
发帖数: 264 | 1 杂志是皇家化学学会的杂志，印象因子是6。
想审的同学请发给我：
1.名字
2.email
3.expertise 关键词（5个左右）
4.发表的文章
谢谢关注，希望大家好运
题目：Redox-triggered intracellular dePEGylation based on diselenide-linked
polycations for DNA delivery
ABSTRACT: Extracellular stability to protect DNA against nucleases and
stimuli-triggered intracellular DNA release are key factors in designing non
-viral gene vectors. In this study, diselenide-linked polycation mPEG-SeSe-
PEI was developed as a new type of PEG-detachable gene vector for redox-
responsive gen... 阅读全帖 |
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t*******s
发帖数: 3031 | 2 我看到Masonic Cancer Center有机子可以做。但机器的使用，培训，注册等只限于他
们的member。挺头疼的。 |
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T*****u
发帖数: 7103 | 3 是confocal laser scanning microscope吗，我做过一个。 |
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T*****u
发帖数: 7103 | 5 streptococcus mutans, red blood cell, human t cell, 水蜗牛，蜗牛的卵，在别处
做的 |
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t*******s
发帖数: 3031 | 6 高手啊，做这么多，做一个大概报多少钱？最近开始了解到这个方法，想用它做一个细
菌生物膜的生长曲线，为模型提供两三个关键参数。测量的思路很简单，方法也都是现
成的，就是几种染色剂染一染，图像处理软件做一下。可惜没亲手摸过机器。。。 |
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l**********1
发帖数: 5204 | 9 那不是没免费下载的点吗 megaupload dead了后
摘自那本CLSM 圣经 free download ok now.
its pp158:
Image processing
Confocal images can be enhanced by
an image processing technique known as
“deconvolution” or “restoration” (Cannell
et al., 2006; Holmes et al., 2006). The purpose
of deconvolution is to remove the blur caused
by diffraction. By measuring or approximating
the point spread function of the optics, one
can mathematically “work backwards” to account
for the distorting effects of the optics on
the true image. ... 阅读全帖 |
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l**********1
发帖数: 5204 | 10 那本CLSM圣经 free download ok now
its pp157
Illumination intensity
Fluorescence emission increases linearly
with illumination intensity up to a level at
which emission saturates. Optimal signal-tobackground
and signal-to-noise ratios are obtained
with illumination levels well below saturation
(Tsien et al., 2006). The illumination
intensity on a laser-scanning microscope can
be adjusted by operating the laser at submaximal
power and by inserting neutral-density
filters into the light path or varying... 阅读全帖 |
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l**********1
发帖数: 5204 | 11 FITC labeling CLSM adjustment method
看图 |
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l**********1
发帖数: 5204 | 12 For LIVE IMAGING
please transfer to LSM 5 Live; Carl Zeiss instrument if your college/university has it.
那本圣经 free download ok now
cited from its pp152
>
A new type of slit-scanning confocal microscope
(LSM 5 Live; Carl Zeiss, Inc.) has
recently been introduced that allows images to
be acquired at rates as fast as or faster than
can be achieved with a spinning-disk confocal
microscope, and with as low or lower rates
of photobleaching. The system adopts principles
from both the spot scanner and ... 阅读全帖 |
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l**********1
发帖数: 5204 | 13 楼主
BAZO two again got
Thank you so much.
还有10th 12th floor revised already from some misleading paste yesterday
please lift up there for get updated Book full text link address.
if you had time should go to below book:
//www.amazon.co.uk/gp/search?index=books&linkCode=qs&keywords=0123740258
Google book link:
//books.google.no/books?id=H--zxc_N-
jMC&printsec=frontcover&hl=no&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
TROUBLESHOOTING
Troubleshooting advice can be found in Table 2. Additiona... 阅读全帖 |
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l**********1
发帖数: 5204 | 14 CLSMs God Father i am beaten by you. |
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l**********1
发帖数: 5204 | 15 没有搞KO mice rat 以及细胞生物的 借用CLSM的薄厚或pre-PhD需要 看懂MIT psf 相
关的PhD thesis
哪怕是摘要的水平
我是搞理论生物物理的
我贴给自己看呢 记录存档用的。please refer 48th floor bottom message.
谁没事在这多费功夫加时间啊
Anyway, thanks LZ BAOZI and his/her null floor paste. |
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a*******y
发帖数: 221 | 16 是一个针对transparent一个针对opaque的specimen么
谢谢 |
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b*****q
发帖数: 114 | 18 狭义的多孔结构推荐用激光共聚焦（CLSM）看
一般水凝胶都是广义的多孔，还没有很好的方法直接表征吧
请教楼上的兄弟，确定小角可以做么？ |
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b*****q
发帖数: 114 | 19 狭义的多孔结构推荐用激光共聚焦（CLSM）看
一般水凝胶都是广义的多孔，还没有很好的方法直接表征吧
请教楼上的兄弟，确定小角可以做么？ |
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