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全部话题 - 话题: conjugates
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W******e
发帖数: 3319
1
来自主题: EB23版 - 体检打针搞不清楚的到这里
http://www.cdc.gov/immigrantrefugeehealth/exams/ti/civil/vaccin
ation-civil-technical-instructions.html#changes
Significant Changes in the Vaccination Requirements
Human papillomavirus vaccine and zoster vaccine are no longer required.
All other age-appropriate, ACIP-recommended vaccines continue to be required.
Significant changes to U.S. immigration vaccination requirements since the
last revision of the "Technical Instructions to Civil Surgeons for
Vaccination Requirements" are—
•Human ... 阅读全帖
S***A
发帖数: 40
2
来自主题: SanDiego版 - SABPA 创业者研讨会 - September 9
The 15th SABPA Entrepreneur Symposium
When: 5:30 pm to 8:30 pm, Tuesday, September 9, 2014
Where: Knobbe Martens at 12790 El Camino Real #100, San Diego, CA 92130
Registration: https://www.123signup.com/register?id=ytcrp
Presentation: Never Be Too Late to Start Up a Biotech Business – Good
Science at a Right Time
Dr. Miao will speak on his successful experience in startup, R&D focus and
business strategy at Concortis Biosystems Corporation, a biotech leveraging
proprietary cytotoxic... 阅读全帖
S***A
发帖数: 40
3
The 15th SABPA Entrepreneur Symposium
When: 5:30 pm to 8:30 pm, Tuesday, September 9, 2014
Where: Knobbe Martens at 12790 El Camino Real #100, San Diego, CA 92130
Registration: https://www.123signup.com/register?id=ytcrp
Presentation: Never Be Too Late to Start Up a Biotech Business – Good
Science at a Right Time
Dr. Miao will speak on his successful experience in startup, R&D focus and
business strategy at Concortis Biosystems Corporation, a biotech leveraging
proprietary cytotoxic... 阅读全帖
s*****t
发帖数: 107
4
来自主题: Biology版 - 求助:Antibody conjuation for FACS
各位大侠,我现在的实验室需要做一些flow cyto的染色。 但是我们实验室只有没有
conjugated的单抗。请问版上有没有大虾会自己conjugate的?我需要conjugate
Biotin或者FITC,PE等。请问你们都用那个公司的Kit?有没有好的protocol?
10个伪币的报答。
H***F
发帖数: 2501
5
mixer 也分passive和active,passive的话是current in,不用conjugate。active的
是voltage in, 也不用conjugate

我的理解是那如果两者之间直接ac couple,因为mixer 的 input impedemce 并不是
LNA output impedance 的 conjugate,所以LNA的 gain 肯定会有所降低吧,那么我们
有必要调整mixer 的size ,是gain 不至于太小吧,而且nf不太大,整个系统的 nf最
小?
v**a
发帖数: 107
6
来自主题: Pharmaceutical版 - PhD Scientist position opening
Department: Biochemistry
Job Title: Scientist
Description:
The department of Biochemistry has an opportunity for a research scientist
to support the preclinical development of antibody-cytotoxic conjugates.
Specific responsibilities include the design and implementation of studies
into mechanisms for both activation and clearance of antibody-drug
conjugates. Additional research efforts will focus on the development of
novel conjugation techniques.
Job Requirements:
Candidates should possess a Ph
c*******g
发帖数: 695
7
来自主题: Pharmaceutical版 - Anyone is currently working on ADC?

至于是site specific conjugation是不是就一定好这是有争议的。很多人说这并不重
~~~~~~~~~~~~~~~~~~~~~~~~~~确实没啥solid的证据
但是从fda看来site specific conjugation好控制质量和生产
所以可能容易批准一些
要。目前为止并没有多少数据支持site specific明显优越于random的conjugation. 对
于stability更重要的是drug load, drug load太高的话stability明显降低。据说
Wyeth的mylotag的问题主要就是这个原因。虽然现在主流意见是当时用的hydrozone
linker不够稳定,但是现在pfizer有个phase 3 ADC用了同样的linker就没有这个问题。
drug
s********n
发帖数: 26222
8
方舟子在此文中说“虫子不吃的, 人能吃吗”是中国人的质疑, 而美国人是不会问这
样的问题的, 这显然是骗人的。见下文和其中引用的文献, 其中不少是美国人的科研

"Comments on the human health impact of Bacillus thuringiensis toxin gene
product in genetically modified crops"
EPA Review Docket Number OPP-00678B
Professor Joe Cummins
Professor Emeritus of Genetics
University of Western Ontario
I have studied the Environment Protection Agency (EPA) document "Bt Plant-
Pesticides Biopesticides Registration Action Document B. HUMAN HEALTH
ASSESSMENT" and comment in the following ... 阅读全帖
k********k
发帖数: 5617
9
来自主题: Military版 - 英语单词 最难发音的10个
http://bbs.creaders.net/education/bbsviewer.php?trd_id=807580
英国人也嫌拗口:最难发音的10个英语单词
送交者: 哆哆 2012年12月25日23:07:25 于 [教育学术] 发送悄悄话
Many have said that sorry is the hardest word but they'd be wrong,
linguistically speaking at least.
很多人说,“对不起”是最难说出口的词。不过,他们其实错了——至少从语言学
的角度上说。
According to a poll, the word we find hardest to pronounce is '
phenomenon.
根据投票调查发现,最难发音的单词其实是“phenomenon”(现象)。
Next in the top 10 of tongue-twisters are 'remuneration', and '
statistics'.
在十个最拗口的词中排在其后的是“remuneration”(报酬)和"stat... 阅读全帖
x*****8
发帖数: 10683
10
来自主题: Military版 - 最讨厌学生和我争论
上次一个美女学生AP Chemistry预测盐溶液的pH。
其中一道题是: Na2SO4
我告诉她:It's exactly 7 since NaOH is a strong base, while H2SO4 is a
strong acid.
当时她将信将疑。
今天她毫不客气地说:I think you were wrong last time. My friends and my
teacher all told me the pH should be higher than 7 since the conjugate acid
of SO4 2- is HSO4 -, which is a weak acid, while the conjugate base of Na +
is NaOH, which is a strong base.
我厌恶地说:If you don't agree with what I said, then please find a
different teacher!
我心里很清楚我是正确的,她的同学和老师都错了!硫酸钠是强酸硫酸和强碱氢氧化钠
... 阅读全帖
x*****8
发帖数: 10683
11
来自主题: Military版 - 最讨厌学生和我争论
这位美女只信她高中AP化学老师教的。
我们在中国高中和大学化学学的原理很简单:
强酸强碱形成的盐不水解,pH中性。
强酸弱碱形成的盐水解,pH偏酸性。
强碱弱酸形成的盐水解,pH偏碱性。
连美国的AP Chemistry大纲上也是这么写的。
这位美女的高中AP化学老师竟然让她先找出这个盐的conjugate base和conjugate acid
,再比较强弱。这个方法既麻烦又不靠谱。

发帖数: 1
12
名字叫舍恩。
德国科学家。不少Nature,Science。后来均被撤稿。
以下摘自维基百科。将军们怎么看这位白人科学家?据一位王姓的华人知名professor
透露,他去演讲,有个欧洲白人老头老是说华人professor的文章是造假造出来的数据
,华人教授气都气死了。
【舍恩事件】
舍恩事件是一起于2002年揭发的大规模学术论文造假丑闻。事件主角扬·舍恩 (Jan
Hendrik Schön) 1970年生于德国费尔登,1997年获康斯坦茨大学物理学博士学位
并留校任教,1998年加入贝尔实验室,于贝尔特拉姆·巴特洛格教授指导下做材料学研
究,2001年成为正式员工。之后的4年里,他与20多人合作在许多大型国际期刊上发表
了超过80篇论文,在《自然》和《科学》上发表的第一作者署名的论文就有17篇。这些
论文在当时被认为于分子电路、分子半导体、有机激光、高温超导和纳米科技领域取得
突破进展,震动了整个科学界。2001年,他获得了德国最重要的科学奖项之一——奥托
·克隆-韦伯银行奖。
舍恩最出名的“成就”是于2001年11月发表的单分子场效应管。他称在两层金电极之间
用含硫的有... 阅读全帖
w********2
发帖数: 632
13
来自主题: Military版 - gmo食品最大的问题
Mobile genetic elements
MGEs are non-essential (accessory) genomic elements composed of genes and
structural features that facil- itate their spread both within and between
organisms (Doolittle and Sapienza, 1980; Orgel and Crick, 1980). They are
found in the genomes of all prokaryotes and eukaryotes. MGEs share many
features and often share a common ancestry with viruses. The main difference
is that viruses are usually capable of extracellular persis- tence.
MGEs, including defective forms, are... 阅读全帖
w********2
发帖数: 632
14
来自主题: Military版 - gmo食品最大的问题
Cellular competency (natural transformation) refers to the active uptake of
free (extracellular) DNA across the cell wall and cell membrane(s) using
cellular machinery designed to facilitate the process (Dubnau, 1999; Lorenz
and Wackernagel, 1994). It is a highly regulated physio- logical state that
is often sensitive to environmental cues (Molin and Tolker-Nielsen, 2003).
When the DNA is her- itably incorporated into the recipient’s genome, the
pro- cess is known as transformation.
Bacterial co... 阅读全帖
I*******l
发帖数: 259
15
来自主题: NextGeneration版 - 宝贝四个月体检小记
宝贝们今天进行了四个月的体检,长势不能说喜人,Just Normal。
天天的体重与身高分别是73%与90%,较两个月时的86%与88%是有升有降。元元的体重与
身高分别是39%与72%,较两个月时的67%与85%,总体都下降了,这就是吃饭不好好吃的
直接后果!
天天基本还是遵循好好吃快快吃多多吃的习惯,既能吃又能睡,总的说来没什么出入。
可元元小家伙就不行了,每次喂奶对我们来说都是艰巨的任务,对他来说是吃玩快乐着
。常常是一边吃一边东顾西望,扭来转去,急了也凶他两句,他还咧着大嘴对你笑,四
盎司的量他总远都是留点尾巴塞不进去,硬塞吧,他可怜巴巴的大眼睛饱含泪水看着你
,发出呜呜哀求声,逼急了,他会奋力反抗,发出杀猪般的叫声,现在的他与三个月前
的小BABY不可同日而语,算了,随他去吧,所以也基本吃多少随他高兴。
今天的结果让我考虑是不是加大他的饭量,但真用上强硬手段又怕他产生厌奶情绪,而
我也是吃力不讨好,所以强硬手段还是得慎用。怎么让他吃得多点呢,这真让人有点纠
结。
四个月还有个坎就是要开始加辅食了,咨询过医生后,还是打算晚点给他们加。实际上
,辅食是从四个月到六个月都可以加,什... 阅读全帖
s******n
发帖数: 37
16
我家小宝6个多月的时候全家搬回国了,回来以后研究了好久怎么在国内续打疫苗有哪
些差别,把研究心得写下来给有需要的家长分享。我们是从加拿大打完6个月的疫苗之
后回去的,加拿大的疫苗和美国略有差别,国内各地的可能也有不一样,仅供参考:)
1. 百白破+小儿麻痹症+B型流感疫苗(Diphtheria,Pertussis,Tetanus,Polio,
Haemophilus Influenzae Type b(Hib)),国内现在有自费的功效一样的五联苗,好像
是790RMB一针,所以这个就不用担心了,回去花钱就可以直接打了。当然国内还有免费
的百破三联针,口服儿麻糖丸,而流感疫苗Hib(安儿宝)不是必须要打的。
2. 乙肝疫苗(Hepatitis B),加拿大是2个月,4个月,6个月三针,国内是出生,1个月,
6个月。
3. 肺炎疫苗(Pneumococcal Conjugate),这个最麻烦,加拿大打的是13价肺炎疫苗(
PCV13),国内有两种,自费的7价疫苗(3个月就可以打)和23价疫苗(2岁以上才能打)
,整个大陆地区都没有13价肺炎疫苗,连那个大家都推荐的和睦家也是7价。13价的包... 阅读全帖
s******n
发帖数: 37
17
我家小宝6个多月的时候全家搬回国了,回来以后研究了好久怎么在国内续打疫苗有哪
些差别,把研究心得写下来给有需要的家长分享。我们是从加拿大打完6个月的疫苗之
后回去的,加拿大的疫苗和美国略有差别,国内各地的可能也有不一样,仅供参考:)
1. 百白破+小儿麻痹症+B型流感疫苗(Diphtheria,Pertussis,Tetanus,Polio,
Haemophilus Influenzae Type b(Hib)),国内现在有自费的功效一样的五联苗,好像
是790RMB一针,所以这个就不用担心了,回去花钱就可以直接打了。当然国内还有免费
的百破三联针,口服儿麻糖丸,而流感疫苗Hib(安儿宝)不是必须要打的。
2. 乙肝疫苗(Hepatitis B),加拿大是2个月,4个月,6个月三针,国内是出生,1个月,
6个月。
3. 肺炎疫苗(Pneumococcal Conjugate),这个最麻烦,加拿大打的是13价肺炎疫苗(
PCV13),国内有两种,自费的7价疫苗(3个月就可以打)和23价疫苗(2岁以上才能打)
,整个大陆地区都没有13价肺炎疫苗,连那个大家都推荐的和睦家也是7价。13价的包... 阅读全帖
t******e
发帖数: 196
18
来自主题: Immigration版 - 也请大家帮忙--推荐审稿机会
也想请大家帮帮忙,给推荐一下审稿的机会。
关键词是: phosphoramidite 的合成, 寡核苷酸/修饰寡核苷酸的合成 (液相,固相
),纯化 (small scale, large scale),鉴定,结构及杂质分析 (UPLC, HPLC,
MALDI, LC-MS, UV, CD...),荧光探针,quadruplex的形成/分析/生物应用,树状分子
设计。
及oligonucleotide cholesterol conjugates, oligonucleotide peptide conjugates
, triphosphate oligonucleotide的合成。
请大牛们帮帮忙推荐一下!有可能的话,请站内联系。
包子答谢!
m****q
发帖数: 118
19
synthesis and conjugation of ZnO nanoparticles
QDs conjugated with serum-biomarker
有兴趣且有能力审稿的朋友请发站内信件:
注明是来自本贴
姓名
联系方式
科研单位及研究小组的link
如果有本人的publication list更好
本贴24小时有效
符合审稿条件的里面
先到先得
谢谢
d******6
发帖数: 276
20
来自主题: Immigration版 - 审稿机会-polymer chemistry
我是做高分子化学药物控释,包括前药这个方向的,求审稿推荐。多谢
Ⅰ: PERSONAL INFORMATION
Name: Xiaoyi XU
Telephone Number: +1-(650)391-5991
E-mail Address: x**[email protected]
Address: Jilin University
Stanford University, School of
Medicine
3801 Miranda Ave, Palo Alto, CA, 94304, US
Ⅱ: EDUCATION EXPERIENCE
YEAR INSTITUTION DEGREES /
2003.09-2006.06 Changchun Institute of Applied Chemistry, Ph... 阅读全帖
n******i
发帖数: 374
21
来自主题: Immigration版 - [review opportunity] area of biomaterial
您好,希望能把这次机会给我,谢谢了
Yarong Liu
2623 Ellendale Pl.
Los Angeles, CA 90007
(213) 249-2419
y*******[email protected]
www.linkedin.com/pub/yarong-liu/2b/a36/65b/
PUBLICATIONS
1. Yarong Liu, April Tai, Kye-Il Joo and Pin Wang. “Visualization of DC-
SIGN-mediated entry pathway of engineered lentiviral vectors in target cells
” 2013, PLoS One 8(6): e67400.
2. Liang Xiao, Kye-Il Joo, Yarong Liu, Jinxu Fang and Pin Wang. “
Injectable thermo-sensitive hydrogel as an adjuvant: in vivo modulation of
dendritic ... 阅读全帖
g******r
发帖数: 512
22
Who can apply
Your spouse, common-law or conjugal partner, or dependent children may be eligible to immigrate to Canada as permanent residents.
An application for Family Class sponsorship can be made if your spouse, common-law or conjugal partner, or dependent children live inside or outside Canada.
The first step is for you to apply as the sponsor. Both you, as the sponsor, and your relative must meet certain requirements.
Applicants for permanent residence must go through medical, criminal and
c******t
发帖数: 1733
23
来自主题: Fitness版 - [bssd] 奔段视频
If the number of lifts deviates significantly from optimal, a de crease in
training effect occurs. This information is found in Managing the Training
of Weightlifters by Laputin and Oleshko.
Let’s look at a simple example. The number of lifts are to be performed on
one of two training days. The light percents are for the development of
explosive, or speed, strength. A few years ago we were using between 50% and
60% of a contest max in the squat. Three lifters used 400 for 12 sets of 2
reps. That... 阅读全帖
c******t
发帖数: 1733
24
来自主题: Fitness版 - [bssd] 奔段视频
If the number of lifts deviates significantly from optimal, a de crease in
training effect occurs. This information is found in Managing the Training
of Weightlifters by Laputin and Oleshko.
Let’s look at a simple example. The number of lifts are to be performed on
one of two training days. The light percents are for the development of
explosive, or speed, strength. A few years ago we were using between 50% and
60% of a contest max in the squat. Three lifters used 400 for 12 sets of 2
reps. That... 阅读全帖
p*********l
发帖数: 26270
25
source:
http://www.astrotheme.com/synastry_advice.php
*** 日月合 Sun-Moon conjunctions
A Sun-Moon conjunction is a very happy indication for the conjugal life and
deep male-female compatibility. Therefore, a female ought to first pay
attention to males whose Sun is on the same position as her Moon, with a 10
or 12 degree orb, or better, a 5 degree orb. The tighter the conjunction,
the stronger the influence.
Conversely, males ought to seek females whose Moon is on the same position
as their Sun, wi... 阅读全帖
wh
发帖数: 141625
26
笑死我了,我去查make-up sex,第一个回答是化妆性别,我以为是男扮女、女扮男。
第二个回答是the sex when making up after an argument, which is best type
other than conjugal visit sex and fugitive sex.我又去查conjugal visit sex,
是说犯人和探狱的妻子。fugitive大概是偷情吧。这都是啥跟啥啊,这么稀奇古怪,哈
哈。你这么一说,我得赶紧去听听adele……
f******x
发帖数: 949
27
来自主题: WaterWorld版 - 关于娶30岁女人,其实说反了。
科普一下conjugal

牛做马,要不然
怎么叫conjugal呢。。呵呵。。。
k********k
发帖数: 5617
28
http://bbs.creaders.net/education/bbsviewer.php?trd_id=807580
英国人也嫌拗口:最难发音的10个英语单词
送交者: 哆哆 2012年12月25日23:07:25 于 [教育学术] 发送悄悄话
Many have said that sorry is the hardest word but they'd be wrong,
linguistically speaking at least.
很多人说,“对不起”是最难说出口的词。不过,他们其实错了——至少从语言学
的角度上说。
According to a poll, the word we find hardest to pronounce is '
phenomenon.
根据投票调查发现,最难发音的单词其实是“phenomenon”(现象)。
Next in the top 10 of tongue-twisters are 'remuneration', and '
statistics'.
在十个最拗口的词中排在其后的是“remuneration”(报酬)和"stat... 阅读全帖
y*****n
发帖数: 210
29
来自主题: AnthroLing版 - 求: 西班牙语教材
I started with <>. But got stuck around lesson 13-14
because the explanation of gustar verbs and reflexive verbs are not clear.
So I started using Duolingo to learn basic vocabulary. It is a very good app
. You can learn simple sentences to pick up basic vocabulary by topic. You
will also see different verb tenses. For that part, you should learn how to
conjugate verbs for different tenses.
Eventually I finished Spanish on Duolingo but I still feel that I don't have
a good... 阅读全帖
d***e
发帖数: 243
30
我来补充一下,不是查google的,是三年前在北医帮导师写教材时我的一些理解.
Transfect一般是指各种artificial的手段给动物细胞内导入外源的基因片段.
Transduction ,conjugation涉及的是细菌间的基因转移,不关真核细胞的事.
1. 转化(Transformation).受体菌直接摄取供体菌游离DNA片段,转化的DNA片段可以是细
菌溶解 释放,也可以是人工提取. 摄取能力称为感受态 competence.应用吗,可以讲是最
早证实了DNA是遗传物质的Griffith的肺炎球菌实验.
2.转导 (Transduction). 温和噬菌体介导的遗传物质从供体菌到受体菌的转移.这里强调
的是temperate phage, 不是指溶原性噬菌体lysogenic phage.作用是细菌自发获得一些
新的protein,eg. toxin or antigen.
3.接合(Conjugation). 是plasmids介导的细胞间接触,是供体菌遗传物质进入受体菌.供
体菌遗传物质不一定就非是供体菌的gene,也可能只是供体菌的质粒transfer到rece
w******e
发帖数: 1187
31
I hope I knew this earlier...:)
I guess you are talking about endosomal escape? or chemical conjugation
simply
abrogate the drug property?
I'm hooked with the simplicity of conjugating aptamer with siRNA. both can
achieve certain level of specificity; both can be synthesized in a single
run; the toxicity profile of ONs are widely available due to the gold
rush in anti-sense field... Yet it's a shame that you only have that few
aptamers to choose from (well, only 1 or 2, according to my knowledge
b******n
发帖数: 4225
32
呵呵,抽质粒老手也碰上这种奇怪的事情
某种Gram-菌的一个isolate/strain,通过conjugation转进了shuttle vector之后
抽质粒用的是Qiagen mini-prep kit
跑电泳发现是很亮的smear
作为对照,该种菌的另外一个isolate/strain同时进行了conjugation和plasmid
extraction得到了清晰的质粒超螺旋条带
做了几次都是这样
现在有点迷糊,不知道可能的问题所在
谢谢指点
n*****n
发帖数: 18
33
来自主题: Biology版 - Assay Development Scientist
Ocean NanoTech seeks result-oriented individuals responsible for actively
participating in on-going research projects, initiating and developing
creative solutions and new products, and promoting collaborations with
academic and industrial partners.
Current Opening:
Assay Development Scientist
Position Description:
This candidate will develop the biological aspects and applications of
nanomaterials for biomedical, environmental, food, and industrial
applications. Responsibilities include:
... 阅读全帖
s*****3
发帖数: 41
34
来自主题: Biology版 - 293T 细胞培养的问题
最近在用293T表达外源蛋白,然后用抗体检测蛋白表达水平。 不知道为什么细胞的
viability非常低,我用7-AAD 染色,viability只有50%。请教实验室前辈这可能是什
么原因导致的呢?
我通常 seed 293T cells the day before transfection at the density of 0.5
million/well (6-well plate).
In the afternoon next day transfect 293T cells with plasmids using FugeneHD.
About 36 hours later, detach cells with trypsin and then stain with the
primary antibody on ice for 30 minutes and then incubate with PE-conjugated
secondary antibodies on ice for 30 minutes. FACS analysis is done
immediate... 阅读全帖
s*****g
发帖数: 87
35
来自主题: Biology版 - Flow cytometry vs western blot
最近要检测一个蛋白的表达量,但是这个蛋白在细胞内的表达量很低
请教各位达人,以下哪个方法更灵敏,或者说更有可能检测到蛋白的表达?
1. Flow cytometry after treatment of FITC-conjugated primary antibody.
2. Western blot analysis using primary antibody and HRP-conjugated secondary
antibody
谢谢!
S**1
发帖数: 35
36
不好意思没用过这个,想跟着问清楚一些:如果用fluorophore conjugated antibody
,那可以用BIO-RAD 的 Gel doc EZ imager 来检测吗? 还是必须买那个licor
odyssey? 还有在检测之前要做什么处理? 是不是直接洗完二抗就行了?另外
fluorophore conjugated antibody(主要是二抗)在哪买最好?谢谢!
t**l
发帖数: 109
37
来自主题: Biology版 - Co IP重链轻链问题
用biotin-conjugate 或者HRP-conjugate 的抗体做WB
n**r
发帖数: 103
38
Ocean NanoTech has an immediate opening for a full time Research Scientist
or part time Consultant to support our development of nanoparticle-based
tools and reagents.
Job Description:
The candidate will be a member of our development team.
1. Study surface chemistry of the particles for various applications and
provide suggestions to our Chemists for surface functionalization/
modification;
2. Conjugate nanoparticles and different biomolecules;
3. Develop nanoparticle-based assays.
Qualificati... 阅读全帖
g***y
发帖数: 201
39
loading control sampler kit from cell signaling.
all antibodies including beta-tubulin ab are HRP conjugated.
very clean, simple, time saving.
β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) cat# 5346
s******s
发帖数: 795
40
来自主题: Biology版 - 包子求赐教-也问streptavidin
想研究protein A与B/C/D等的相互作用。
因为没法(或者很难)把蛋白A偶联到sepharose beads上,但是有commercially
available biotinylated protein A可以直接买(sepharose conjugated with
streptavidin当然也有卖),所以计划通过streptavidin+biotin搭桥,间接把protein
A
结合到sepharose beads上(sepharose-streptavidin + biotin-protein A),然后装
填柱子,然后apply protein B/C/D。
从来没做过avidin+biotin相关的实验,请问有朋友做过类似的么?streptavidin+
biotin的结合够强壮么?
假设 protein C可以与protein A结合,最后形成sepharose-streptavidin + biotin-
protein A + protein C。在wash和elute的时候,有特定的或者通用的条件可以
1。wash掉非特异结合的其他protein
2。特... 阅读全帖
w******e
发帖数: 1187
41
来自主题: Biology版 - paper help
Antibody-Drug Conjugates
Volume 1045 of the series Methods in Molecular Biology pp 1-27
Date: 01 July 2013
Antibody–Drug Conjugate (ADC) Clinical Pipeline: A Review
Could you please send it to my email: [email protected]
/* */
Thanks a lot!
a*****c
发帖数: 53
42
Rise to prominence[edit]
Schön's field of research was condensed matter physics and
nanotechnology.[3] He received his Ph.D. from the University of Konstanz in
1997. In late 1997 he was hired by Bell Labs, located in New Jersey, United
States. There, he worked on electronics in which conventional semiconducting
elements (such as silicon) were replaced by crystalline organic materials.
Specific organic materials can conduct electrical currents, and in a so-
called field-effect transistor (a ... 阅读全帖
d****i
发帖数: 360
43
来自主题: Chemistry版 - Help me, Please! Thanks a lot!
E-mail: x**********[email protected]
Synthesis of a Macromolecular Camptothecin Conjugate with Dual Phase Drug
Release
Author: Synthesis of a Macromolecular Camptothecin Conjugate with Dual Phase
Drug Release Yurkovetskiy, A. V.
Journal: Molecular pharmaceutics
ISSN: 1543-8384 Date: 2004
Volume: 1 Issue: 5 Page: 375
p**********n
发帖数: 329
44
来自主题: Chemistry版 - 求book chapter, wiley
3. Regioregular and Regiosymmetric Polythiophenes, in Conjugated Polymer
Synthesis: Methods and Reactions
Prof. Yoshiki Chujo
DOI: 10.1002/9783527632664.ch3
Link: http://onlinelibrary.wiley.com/doi/10.1002/9783527632664.ch3/summary
2. Catalyst-Transfer Condensation Polymerization for Precision Synthesis of
π-Conjugated Polymers
Prof. Yoshiki Chujo
DOI: 10.1002/9783527632664.ch2
Link: http://onlinelibrary.wiley.com/doi/10.1002/9783527632664.ch2/summary
多谢帮助!
p**********n
发帖数: 329
45
来自主题: Chemistry版 - 球paper
3. Regioregular and Regiosymmetric Polythiophenes, in Conjugated Polymer
Synthesis: Methods and Reactions
Prof. Yoshiki Chujo
DOI: 10.1002/9783527632664.ch3
Link: http://onlinelibrary.wiley.com/doi/10.1002/9783527632664.ch3/summary
2. Catalyst-Transfer Condensation Polymerization for Precision Synthesis of
π-Conjugated Polymers
Prof. Yoshiki Chujo
DOI: 10.1002/9783527632664.ch2
Link: http://onlinelibrary.wiley.com/doi/10.1002/9783527632664.ch2/summary
多谢帮助!
t******e
发帖数: 196
46
也想请大家帮帮忙,给推荐一下审稿的机会。
关键词是: phosphoramidite 的合成, 寡核苷酸/修饰寡核苷酸的合成 (液相,固相
),纯化 (small scale, large scale),鉴定,结构及杂质分析 (UPLC, HPLC,
MALDI, LC-MS, UV, CD...),荧光探针,quadruplex的形成/分析/生物应用,树状分子
设计。
及oligonucleotide cholesterol conjugates, oligonucleotide peptide conjugates
, triphosphate oligonucleotide的合成。
请大牛们帮帮忙推荐一下!有可能的话,请站内联系。
谢谢!
C**R
发帖数: 1047
47
来自主题: Chemistry版 - 量子化学已经接近完全死亡了
你丫就是想说tight binding是a very simple linear combination of atomic
orbitals molecular orbitals?
The Hückel method or Hückel molecular orbital method (HMO) proposed by
Erich Hückel in 1930, is a very simple linear combination of atomic
orbitals molecular orbitals (LCAO MO) method for the determination of
energies of molecular orbitals of pi electrons in conjugated hydrocarbon
systems, such as ethene, benzene and butadiene.[1][2] It is the theoretical
basis for the Hückel's rule; the extended Hückel m... 阅读全帖
W*****s
发帖数: 684
48
来自主题: Chemistry版 - Help me please! Thanks!
Preparative and Analytical Separation of Insulin-Dextran Conjugates From
Native Insulin: Application to Preparation and Characterization of Insulin-
Dextran Conjugates; Drug Development and Industrial Pharmacy; 1994, Vol. 20,
No. 3 , Pages 395-404 (doi:10.3109/03639049409050192)
Read More: http://informahealthcare.com/doi/abs/10.3109/03639049409050192
Thanks a lot!
m****q
发帖数: 118
49
【 以下文字转载自 Immigration 讨论区 】
发信人: mbdshq (mbdshq), 信区: Immigration
标 题: 审稿机会:氧化锌纳米颗粒的合成及其与牛血清白蛋白的偶联
发信站: BBS 未名空间站 (Sat Feb 11 15:12:14 2012, 美东)
synthesis and conjugation of ZnO nanoparticles
QDs conjugated with serum-biomarker
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