w*****s
发帖数: 122 | 1 How do I send the correct MIME/HTTP headers using Apache .htaccess files? (Not
finished)
If you are using a recent version of the Apache server, then
your Webmaster must give you "AllowOverride FileInfo"
permission. Then you can put a file called .htaccess in
any directory. (Note that in MIME terminology, "encoding" means
"compression". In the XML encoding header, "encoding" means "
coded character set") Here is a line that may be useful--
DefaultLanguage zh
AddType application/xml XML xml
Why i |
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b********r
发帖数: 9 | 2
how
1.extract the total mRNA
2.use restriction enzyme to cut out the desired gene
3.design a primer and do a PCR
4.Sequence the DNA and compare with those published sequences to see whether
it encodes the kind of enzyme you want.
5.Southern Blot to see whether pure and whether it is there.
6.Express the cDNA in Ecoli and check protein activity.
7.translate the sequenced cDNA to see whether you got the correct protein.
8.Sequence the expressed protein and back translate part of the AA's that has |
|
p********i
发帖数: 116 | 3 问一个统计问题，reviewer 问有没有用什么statistical correction ，这个是什么意
思？谢谢。 |
|
p********i
发帖数: 116 | 4
谢谢您，他是这样问的，
Given all the comparisons that were made in the article was any form of
statistical correction applied? A significance level of P<0.05 should be
noted that
all values are uncorrected.
看不懂这个问题， |
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s******s
发帖数: 13035 | 5 are you sure it is "correction, uncorrected"
not "correlation, uncorrelated"? |
|
s**f
发帖数: 365 | 6 Multiple testing will increase the chance of observing false results (i.e.
observing a difference purely by chance).
some corrections used to the significance level:
Bonferroni
Holms
Tukey
Dunnet |
|
c****d
发帖数: 501 | 7 测序以后发现一个点突变
如果不想重新做构建的话
有什么办法可以correct it？
谢谢啦 |
|
N2
发帖数: 81 | 8 1)screen 3-5 more colonies and verify the sequence of that site by
sequencing.
2)if there is no correct one, it seems the fragment used for ligation having
that mutation.
3) do a reverse PCR to fix it if you plasmid is not too big.
4)site mutegensis by Quick Change
5)rebuild the plasmid with new fragments from PCR using Phusion DNA
ploymerase
from 3) to 5) find any one which will be easy for you.
good luck! |
|
r*******y
发帖数: 48 | 9 easiest way is to do site mutagenesis to get it back to the correct one. |
|
c****d
发帖数: 501 | 10 是这样
从质粒1里边扩增片段A
从质粒2里边扩增片段B
然后把A和B连起来 再接到vector里边
其中片段A还比较大
现在就一个mutation 所以我想如果有简单办法correct的话
就不用重新来一遍了 |
|
B**U
发帖数: 220 | 11 各位牛人，有了解超分辨显微镜movie里的不同时间的图像X-Y Drift Correction的么
？ 庄小薇的程序是Python语言的，不会用啊！
多谢！ |
|
c*****t
发帖数: 198 | 12 【 以下文字转载自 Engineering 讨论区 】
发信人: compact (support), 信区: Engineering
标 题: paper help (转载)
发信站: BBS 未名空间站 (Thu Sep 9 19:28:24 2010, 美东)
发信人: compact (support), 信区: Biology
标 题: paper help - sorry, title corrected
发信站: BBS 未名空间站 (Thu Sep 9 19:27:16 2010, 美东)
Coarse-Grained Elastic Models of Protein Structures for Understanding
Their Mechanics and Dynamics
Authors: Eom, Kilho; Yoon, Gwonchan; Kim, Jae-In; Na, Sungsoo
Source: Journal of Computational and Theoretical Nanoscience, Volume 7,
Number |
|
|
c***z
发帖数: 6348 | 14 【 以下文字转载自 PoliticalScience 讨论区 】
发信人: chaoz (面朝大海，吃碗凉皮), 信区: PoliticalScience
标 题: survey bias correction问题 (转载)
发信站: BBS 未名空间站 (Sat Jan 18 13:46:16 2014, 美东)
请教一下达人们，你们做survey的时候，怎么adjust for selection bias啊？
能给个paper的link么 |
|
f******e
发帖数: 921 | 15 I want to solve a system of ODE, for example,
x1'=x2-x1^2+x3^2
x2'=-x1*x3-2*x1*x2+x3
for x3, it satisfy, x1^2 + x2^2+x3^2=10;
my code is below:
function xp = F(t,x)
xp = zeros(3,1);
xp(1)= x(2) -x(1)^2+ x(3)^3;
xp(2) = - x(1)*x(3) - 2*x(1)*x(2) + x(3);
x(3) = sqrt(10-x(1)^2-x(2)^2);
[t,x]=ode45(@F,[0,10],[0;1;1]);
plot(t,x)
my figure shows x3 is a straight line, what is the mistake?
How to correct the mistake in my code? thank you very much!!! |
|
g*****e
发帖数: 16 | 16 C is correct.
E is incorrect, because the lawyer argues that violent character is
necessarily associated with violent crime. Quite opposite to E. |
|
n**0
发帖数: 244 | 17 In fact, nothing is correct. |
|
n**0
发帖数: 244 | 18 I know the fact. I also know "罗素悖论" is not correct. |
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o*******w
发帖数: 349 | 19 "Correction to 'entropy and maximal spacings for random partitions'"
by Eric Slud
Probability Theory and Related Fields, Volume 60, Number 1 (1982)
请发至
M*************[email protected] |
|
b*****u
发帖数: 289 | 20 218 in real test, NBME correct questionsX1.5-10. not bad. |
|
F*****g
发帖数: 31 | 21 我手头有一个旧的, 但是感觉不准了, 如下
correct answer, USMLE 3digit,
80.1% 225
85.2% 238
90.1% 250
各位前辈,你们大概拿到多少分考step1 呢？ 以NBME form 1-7为样板， 最后成绩大概
需要多少。要90%吗，我现在只有80%，不知道有何好的建议，计划两周内考。
非常感谢。 |
|
F*****g
发帖数: 31 | 22 能给我一个正式的分数换算表吗，correct answer - USMLE,
这样我摸考时心里有底 |
|
x********g
发帖数: 595 | 23 He is a very honest guy who never hesitates to tell the truth. You will
never find any stupid self-censored political correctness in his blog posts. |
|
S*********f
发帖数: 1938 | 24 ☆─────────────────────────────────────☆
alvarado (治孤) 于 (Wed Apr 26 00:19:48 2006) 提到:
http://www.ourcivilisation.com/pc.htm
☆─────────────────────────────────────☆
alvarado (治孤) 于 (Wed Apr 26 00:39:22 2006) 提到:
这个网页讲得很有趣
PC原来是为实现各群体平等而形成的norm，最后有可能成为压制言论自由，这段话特逗：
By using the excuse of not upsetting anyone, the politically correct are
demanding that people behave like the fool who would please everyone; that
everyone must become such a fool! All must accept the notions of the
Politica |
|
c***z
发帖数: 6348 | 25 【 以下文字转载自 PoliticalScience 讨论区 】
发信人: chaoz (面朝大海，吃碗凉皮), 信区: PoliticalScience
标 题: survey bias correction问题 (转载)
发信站: BBS 未名空间站 (Sat Jan 18 13:46:16 2014, 美东)
请教一下达人们，你们做survey的时候，怎么adjust for selection bias啊？
能给个paper的link么 |
|
c********8
发帖数: 5 | 26 有一个关于 RMA background correction 的公式：
Model S as the sum of a signal X and a background Y, S=X+Y, where we assume
X is exponential (α) and Y is Normal (µ,σ2), X, Y independent
random variables.
Background adjusted values are then E(X|S=s), which is
a + b[f(a/b) - f((s-a)/b)]/[F(a/b) - F((s-a)/b) - 1],
where a = s - µ- ασ2, b = σ, and f and F are the normal
density and cumulative density, respectively.
请提供一些推导此公式的线索。谢谢！ |
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s**m
发帖数: 340 | 27 两组病人A and B， 每组又按时间分为Event 和 Ctrl,也就是共有4组数据：Event_A,
Ctrl_
A, Event_B, Ctrl_B. 然后进行了如下比较:
Event_A vs. Event_B
Event_A vs. Ctrl_A
Event_B vs. Ctrl_B
Ctrl_A vs. Ctrl_B
在上述比较中，每组数据被比较了2次，共4次比较。在进行Bonferroni correction的
时候，比较次数应该是2还是4？
|
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o*******w
发帖数: 2310 | 28 A raw data file is listed below.
----|----10---|----20---|----30
son Frank 01/31/89
daughter June 12-25-87
brother Samuel 01/17/51
The following program is submitted using this file as input.
data work.family;
infile file-specification;
run;
Which INPUT statement correctly reads the values for the variable BIRTHDATE
as SAS date values?
A. input relation $ first_name $ birthdate date9.;
B. input relation $ first_name $ birthdate mmddyy8.;
C. input relation $ first_na... 阅读全帖 |
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S********f
发帖数: 36 | 29 Spherical surface samples are measured for their radii (R) and imaged by a
camera. The image is not a numerical value, but m different quantities (Q1,
Q2,..., Qm) are derived from it using different models. Now if I want to
analyze the correlation between R and each Qi (i = 1,..., m), should
Bonferroni correction be applied to adjust the significance level? Thanks! |
|
c****t
发帖数: 19049 | 30 没太看明白。Ｃ是权威？不是有的site跟random也差不多么？为神马认定你的结果是
biased?
那个global ratio是当年没办法时的办法。这种情况下可能性较大的是你这个dataset
里QEL互不同意的部分的实际male ratio挺接近35%
如果认定你的结果是biased的，correction用shrinkage/penalty那套。philosophy是
说misclassification/residuals这些不是垃圾而是跟model同样重要甚至更重要的东西
。要解决的是理论上不存在的global optimized solution。具体算法你不会写的话最
简单的办法是把misclassification硬性重model一遍，再model misclassification的
misclassification 。。。直到你满意为止。只要你们公司没人懂quality control理
论，把这些models一凑就行了 |
|
c***z
发帖数: 6348 | 31 This is the main project I am working on. I would greatly appreciate if you
can share some insight. :)_
Say you have huge data set about online activities, you know that the data
set is biased, i.e. most of the information are collected from young males
making less than 50K. However you don't know how much is the bias.
To sell data products to online marketing companies, you need to correct for
the bias, so that your products work for the whole online population, or a
different population, such ... 阅读全帖 |
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s*********e
发帖数: 1051 | 32 1. build a lookalike model to predict the likelihood of young male and
derive the propensity score
2. use the propensity score to correct the bias
you
for
a |
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m***o
发帖数: 225 | 33 我前段时间做过一个相似的项目。基于一个google research发的paper。你可以search
一下。
题目大概是bias correction theory
you
for
a |
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c***z
发帖数: 6348 | 34 Just did a second round and it was way off.
Here is what I did. Any input is extremely welcome!
Business objective: Correct for panel bias in terms of demographic breakdown
, to obtain an accurate multiplier, in order to calculate site traffic,
sales, etc
Input data:
1. Site visits by people (own data, company Q);
2. Third party (C) 2000 site gender decomposition;
3. Third party personal gender labels (two companies, E and L);
Technical logic:
1. Assign a score to sites based on thei... 阅读全帖 |
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c***z
发帖数: 6348 | 35 我们的panel是US population的10%，然后我们只知道panel中10%的人的性别，所以“
有性别信息”这一点可能也带来了新的bias。
（而且这10%的性别信息来自3个来源，这些来源相互矛盾而且自相矛盾。）
就是说要用1%的sample去correct 10%的sample的bias。
目前的想法是从这10%人群中bootstrap一个性别1：1的子集作为基准。。。
感觉不管怎样都是shoot in the air，头疼。
too |
|
c***z
发帖数: 6348 | 36 Hi all,
First thank you all so much for your inputs! They were extremely helpful!
Here is what we are doing as a second try (actually maybe 5th try, but we
only count major overhauls here).
Again, any input is extremely welcome! Thanks!
Situation Brief
Project name: Bias correction
Business objective: We have a panel of 25M users’ shopping cart information
, we want to infer national online sales by brand and channel. We do so by
finding and applying multipliers to each shopping cart item, based... 阅读全帖 |
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p****o
发帖数: 1340 | 37 It is a very interesting question. Of course, after reading the whole post,
some background information is still lacking. So, I just randomly throw some
ideas around.
1. At which level, the analysis is to put on? How about brand/channel level
since it is the level of your interest if my understanding is correct.
If you can employ a drill-down approach, then it is possible to expand it to
an individual level later on -- like a hierarchical partition -- without
major overhaul to the code base.
2. ... 阅读全帖 |
|
|
w*******y
发帖数: 60932 | 39 One for the Men and the Ladies of SD...
High-end 'Anatomically Correct' Mens underwear.
Coupon code: 50NAKED12 good for 50% off the Almost Naked collection.
Shipping looks to be around $9 for USPS priority. There doesn't appear to go
up with a few pair in the cart, so if these are for you buying more than
one in an order helps.
Link:
http://www.andrewchristianshop.com/Almost-Naked-at-Andrew-CID29
|
|
w******e
发帖数: 1038 | 40 I think there will be another big drop coming, all corrections previously
are zigzags, it is just forming the first strike yet. |
|
c*******u
发帖数: 12899 | 41 ☆─────────────────────────────────────☆
GoodToCU (无心) 于 (Wed Apr 25 16:40:16 2012, 美东) 提到:
认为给孩子保留中国国籍选项无所谓的父母请跳过。
如果你的孩子在美国出生的时候你们还没有拿到绿卡。
按国籍法第五条，你的孩子有中国国籍。
然后你们拿绿卡了并不代表孩子中国国籍
的自动丧失。按国籍法第十四条规定：
“中国国籍的取得、丧失和恢复，除第九条规定的以外，
必须办理申请手续。未满十八周岁的人，可由其父母或
其他法定代理人代为办理申请。”
所以如果可能请为你的孩子申请旅行证而不是回国的
签证。如果签证，以后我国政府可能说申请签证就相当
于丧失中国国籍的申请手续。
另外国籍法第十七条：
第十七条本法公布前，已经取得中国国籍的或已经丧
失中国国籍的，继续有效。
=========================
这个主题里已经有长达142楼的讨论。下面是我
看完后的UPDATE：
-----------------------------------
发信人: GoodToCU (无心), 信区: ... 阅读全帖 |
|
G******i
发帖数: 5226 | 42 ☆─────────────────────────────────────☆
Sandra720 (I am the real Sandra) 于 (Fri Jun 15 10:06:50 2012, 美东) 提到:
看不太懂使馆的要求，打电话永远没人接。情况是：娃在美国出生，娃爹入了美国籍，
娃妈刚绿卡批准，应该是中国公民。这种情况娃是不是要办旅行证？可我见使馆要求“
父母双方或一方为中国公民，本人出生在外国，具有中国国籍，前往中国时应申办中国
旅行证件”娃10个月，怎么证明娃有中国国籍？
☆─────────────────────────────────────☆
mua (mua) 于 (Fri Jun 15 10:14:55 2012, 美东) 提到:
你有绿卡了娃就得办签证了。
根据《中华人民共和国国籍法》，父母双方或一方为中国公民，本人出生在外国，具有
中国国籍，前往中国时应申办中国旅行证件。父母双方或一方为中国公民并定居在外国
，本人出生时即具有外国国籍的，不具有中国国籍，前往中国时应申办中国签证。
☆──────────────────────... 阅读全帖 |
|
f**d
发帖数: 768 | 43 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
|
c*******u
发帖数: 12899 | 44 ☆─────────────────────────────────────☆
Truthtry (chloe) 于 (Thu Sep 13 01:15:14 2012, 美东) 提到:
有绿卡，但不想入籍，小朋友还有可能去领事馆办旅行证不办签证回国吗，谢谢
☆─────────────────────────────────────☆
Correct (Correct) 于 (Thu Sep 13 12:50:10 2012, 美东) 提到:
只要小朋友出生时父或母尚未取得绿卡即可。
☆─────────────────────────────────────☆
familytree (会疯的淑女) 于 (Thu Sep 13 16:39:28 2012, 美东) 提到:
有绿卡的生出来就是美国公民了嘛
☆─────────────────────────────────────☆
zzbaby (小兔~双宝妈) 于 (Thu Sep 13 17:57:58 2012, 美东) 提到:
问题是中国领事馆怎么知道我在这里有没有绿卡啊？
☆───... 阅读全帖 |
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T*R
发帖数: 36302 | 45 工会和行会是保护劳工利益的坚强手段：
ILLINOIS一共有115个LEVEL 2 STATE监狱护士，他们的工资网上能查到：
CORRECTIONS NURSE II
Total positions: 115
Total payroll: $11,131,269.64
Name
County of Residence
Department
Salary
COLEMAN LOREATHA A Cook DEPARTMENT OF CORRECTIONS $242,222.95
IFEZUE NWADIUTOR O Cook DEPARTMENT OF CORRECTIONS $201,932.11
SALAKO-OJEI HELEN W Cook DEPARTMENT OF CORRECTIONS $173,051.28
CLAUS ELLEN K Pike DEPARTMENT OF CORRECTIONS $161,182.25
ONWUEYI SAMPSON I Cook DEPARTMENT OF CORRECTIONS $153,169.34
MCCALLUM DE... 阅读全帖 |
|
s*****5
发帖数: 69 | 46 找网上驾校，版上推荐gototrafficschool.com, 找历史题目贴，安全通过。final
Exam只能考两次有点紧张，但一次就过了，现将最后考试题目回馈mitbbs.
Congratulations!
You passed the final exam!
You got 25 questions correct.
Review the Correct Answers
1. Diamond lanes, with the exception of motorcycles and certain hybrid
vehicles in California, are reserved for vehicles with:
A. children
B. the elderly
Correct C. two or more people
D. four or more people
2. ________ refers to speeding without realizing it.
A. highway hypnosis... 阅读全帖 |
|
x*******1
发帖数: 28835 | 47 Golden State Warriors | 35-27 (20 games left, Currently 6th)
Opponent | Prediction | Result
Mar 8 vs HOU | Win | Loss <--- happy that i was wrong
Mar 9 vs MIL | Win | Loss <--- Wrong again but i'm not complaining.
Mar 11 vs NYK | Win | Win <--- Correct
Mar 13 vs DET | Win | Win <--- Correct
Mar 15 vs CHI | Loss | Loss <--- Correct
Mar 17 @ HOU | Loss | Win <---
Mar 18 @ NOH | Win | Win <--- Correct
Mar 20 @ SAS | Loss | Loss <--- Correct
Mar 23 vs WAS | Win | Win <--- Correct
Mar 25 vs LAL | Win... 阅读全帖 |
|
l****z
发帖数: 29846 | 48 Policeman on Brown: 'He Grabbed My Gun, He Twisted It, Pointed at Me'
November 25, 2014 - 12:26 PM
By Brittany M. Hughes
(CNSNews.com) – One day after fatally shooting Michael Brown, an unarmed
black teenager, Ferguson, Mo., police officer Darren Wilson, who is white,
gave his account of the incident to two detectives from the St. Louis County
Police Department, explaining in detail how Brown attacked him through the
police car’s open window, wrestled for the officer’s gun, and then pointed
it... 阅读全帖 |
|
b*****n
发帖数: 482 | 49 Suppose we randomly pick a, b, c, and d, then
if a/d is correct, the chance to be correct is 50%
if b is correct, the chance to be correct is 25%
if c is correct, the chance to be correct is 25%
Let P(a/d) to be the possibility of a/d is the correct answer
P(b) to be the possibility of b is the correct answer
P(c) to be the possibility of c is the correct answer
and P(total) to be the possibility of getting the correct answer
Then we have
P(total) = P(a/d) * 50% + P(b) * 25% + (1-P(a/d)-P(b)... 阅读全帖 |
|
f********x
发帖数: 99 | 50 The world beyond batch: Streaming 101: A high-level tour of modern data-
processing concept
http://radar.oreilly.com/2015/08/the-world-beyond-batch-streami
by Tyler Akidau August 5, 2015
Editor’s note: This is the first post in a two-part series about the
evolution of data processing, with a focus on streaming systems, unbounded
data sets, and the future of big data.
Streaming data processing is a big deal in big data these days, and for good
reasons. Amongst them:
Businesses crave ever more tim... 阅读全帖 |
|
