t*****k
发帖数: 390 | 1 For a undirected graph, how can we detect whether there is a cycle using
minimum space? We do not care how much time it will take and the graph is
stored in the form of adjacent lists.
THANKS!! |
|
b***i
发帖数: 3043 | 2 我们是个嵌入式项目，由于有硬件和软件，公司使用Life Cycle。在Gate 2的时候，需
要一个完整的项目计划，人数和资金都预测好。然后，必须在规定的时间完成。晚了两
周要罚，比如不能升职等。
大家的软件项目都是怎么管理的？我们软件组也是这样，但是到期他可以说我完成了，
其实很多feature没有做。 |
|
r*****1
发帖数: 27 | 3 个人觉得在真正考试中，transaction cycle的考题少到几乎可以不用准备这一块了。 |
|
r*****1
发帖数: 27 | 4 个人觉得在真正考试中，transaction cycle的考题少到几乎可以不用准备这一块了。 |
|
x********2
发帖数: 326 | 5 Becker is good enough
个人觉得在真正考试中，transaction cycle的考题少到几乎可以不用准备这一块了, I
took AUD twice |
|
m**********d
发帖数: 137 | 6 我在用一个作用于G1/S checkpoint的药物处理细胞，现在需要用FACS看一下cell
cycle distribution。向前辈们请教一下，这种情况需要synchronize我的细胞吗，或
者什么情况下需要做synchronization? 非常感谢 |
|
s******u
发帖数: 81 | 7 如果药物处理导致的G1/S block时间超过一个cell cycle的时间, 就不用
synchronization. |
|
B******f
发帖数: 2489 | 8 也许不够确切吧。
如果你再re-introduce E2F1, 在为drug未处理对照组有相似E2F1 level情况下，可以
rescue或部分rescue drug引起的cell cycle arrest, 应该更有说服力。 |
|
f**g
发帖数: 28 | 9 Hi, everyone, is there any connection between cell cycle and transfect
efficiency. Why there are different expression level of tranfected genes in
different cells? Thanks for any suggestion. |
|
c**k
发帖数: 1228 | 10 CELL cycle. the editorial board is very strong |
|
s**********e
发帖数: 2888 | 11 I was asked one question about this cycle in my preliminary exam, and can
not answer, then was told to read basic biochemistry textbook again. |
|
s****m
发帖数: 472 | 12 Hello Everyone,
Anybody studied cell cycle here? Is there any assays or markers to
distinguish G2 phase from M phase easily?
Thank you so much! |
|
I*****y
发帖数: 6402 | 13 已经export fcs文件了，而且电脑上有flowjo软件，但是不知道怎么做3d 图显示cell
cycle progression。请给个简单的做法？谢谢 |
|
q**********0
发帖数: 335 | 14 Lipofectamine 2000 转染细胞后，发现细胞形态学变化很大，可能是由于
Lipofectamine 2000的高细胞毒性造成的吧。请教， Lipofectamine 2000 转染的细胞
适合作cell cycle analysis 吗？Thanks |
|
o***e
发帖数: 130 | 15 我想看一个蛋白在不同cell cycle(G0, G1, S, M)时期的distribution。用
fluorescence microscopy, 有什么marker或其他分辨方法吗？谢谢！
实在不行可以先sort，是下策了。 |
|
C******g
发帖数: 40 | 16 Does anyone know which NIH grant cycles are decided based on 2013 budget?
July 2012 submission (scored in Oct. 2012), October 2012 submission (scored
in Feb/March. 2013), February 2013 submission(scored in July. 2013)? Does
this mean February submissions usually have a shorter waiting time for a
final decision (funded or not)?
This website is usually but does not explain everything:
http://www.niaid.nih.gov/researchfunding/paybud/pages/planoppsb |
|
C******g
发帖数: 40 | 17 Does anyone know which NIH grant cycles are decided based on 2013 budget?
July 2012 submission (scored in Oct. 2012), October 2012 submission (scored
in Feb/March. 2013), February 2013 submission(scored in July. 2013)? Does
this mean February submissions usually have a shorter waiting time for a
final decision (funded or not)?
This website is usually but does not explain everything:
http://www.niaid.nih.gov/researchfunding/paybud/pages/planoppsb |
|
w**e
发帖数: 2358 | 18 请教本版同学， changing cycling conditions对PCR结果大吗？ 比如说95度40个
repeats,每个repeat 3s vs 15s? 谢谢啦～ |
|
A******y
发帖数: 2041 | 19 Not only that, is your primers still linear at cycle 30-36? Did you check
the linearity of the primers? I know a lot of people don't do qPCR
correctly. |
|
h*********g
发帖数: 18 | 20 I am using Taqman primer. Do you think there are some problems? Thanks.
cycles |
|
h*********g
发帖数: 18 | 21 Here is my cycle numbers corresponding to 2X dilution. How do you think?
Thanks.
28.8, 30.13, 31, 32.74, 33.86
I do not have positive control and can only use my sample.
take
PCR |
|
A******y
发帖数: 2041 | 22 How about you tell me. If you draw a standard curve, is it linear? from 31
to 32.74, you have about 2 cycle difference and you only diluted by x2.
Unless you skip a point. You should also do more points. |
|
F******p
发帖数: 2099 | 23 >32 is not reliable.
I suspect you were using 2 primers qPCR. Try 2 primers and probe based qPCR
, that is way more reliable.
cycles |
|
A******y
发帖数: 2041 | 24 I am just sad that the PI don't train people properly, you should always
validate your primer regardless what people told you. If you have controls,
high cycle range is not a problem. |
|
c********y
发帖数: 98 | 25 Barry Naughton recently wrote an open letter to Wen Jiabao in the Foreign Policy magazine.
One of the more interesting things he mentiones is that the current economic heating
actually started in 2001, and it was initiated in preparation for the 16th party
congress in 2002. Do we really have this business cycle in China? 1987/88, yes;
1992/1993, yes; 1997/98? |
|
c********y
发帖数: 98 | 26 yeah, there was no overheating in 97/98, so this business cycle model didn't
work for that party congress. Intuitively, however, the idea that the
government supplies policies to generate growth at a time of party congress or
other significant events sounds appealing. And we do know that CCP does it
from time to time. Wonder if something consistent can be worked out (not
necessarilty related with party congress).
consistently
abnormally |
|
l******n
发帖数: 213 | 27 景气循环不就是。。。business cycle吗。。。难道不是吗。。。
然后理论。。。
xiaokai好像不是很主流吧。。。
(至于中国经济，我觉得那是political以及reform的问题。) |
|
l******n
发帖数: 213 | 28 汗。。。手册搞到手这么久一直都没看过宏观那几本。。。泪啊。。。
我个人比较喜欢金融和经济增长\波动。
关于cycle理论有米有比较updated一点的综述？ |
|
i*******e
发帖数: 349 | 29 忘记说了，SHLEIFER有篇86年的JPE文章，叫做Implementation cycles。FRANCOIS和
LLOYD-ELLIS的AER文章Animal Spirits Through Creative Destruction讲Creative
Destruction如何可能引起周期。
我个人感觉这些是都是可能的内在机制，但和经济周期的实证检验还有一定距离。 |
|
g******u
发帖数: 3060 | 30 有是有的，那个linear tech的芯片就号称可以。原文写
In PWM operation an on-chip switching regulator maintains charge in the
bootstrap capacitor even when approaching and operating at 100% duty cycle.
IR的tech note给了个电路，用块555可以实现。基本原理是一个cap放电时负载charge
另外一个cap,然后这两个cap互相对充。 |
|
g******u
发帖数: 3060 | 31 有两个digital输入，大约5V吧。各自通过optoisolator驱动一个电机。两个输入分
别对应电机的快速和慢速，也就是接入不同的winding.相当于一个SPDT.
问题是这两个输入必须有一定的dead time, 不然有一点overlap的话会使winding短
路。可以用很简单的逻辑电路或者用一块能改dead time的half bridge driver来实现
。不过为了安全起见，希望这个delay可以长一点。
也就是说，如果两个输入都是high,那么希望其中的一个会被延迟到几个AC cycle以
后再触发。
有无建议?多谢。
|
|
ET
发帖数: 10701 | 32 想不出还能有其它办法。
觉得总要找个reference clock有这么个50% duty cycle的。
pll其实也没那么复杂。 |
|
c******h
发帖数: 454 | 33 divide clock by 2 and use a DLL to align the rising edge with falling edge
and then shift 90 degree. XOR the divided clock and shifed 90 degree clock,
you will get duty cycle correction.It is a typical DCC circuitry. |
|
D******D
发帖数: 1022 | 34
don't think so, this cycle is buyer's market, not enough students are
attending. Every school is trying its best to keep the number up.
"Even though Michigan increased its acceptance rate this year from the
traditional one in five applicants to one in four, its incoming class still
may end up slightly smaller than last year, she said."
"Zearfoss said that one law school, which she declined to identify, offered
half-tuition scholarships to each wait-list applicant they decided to offer
admission... 阅读全帖 |
|
z******r
发帖数: 8 | 35 本人在MS和PhD阶段有大量发动机和燃烧研究背景，近期也积累了不少waste heat
recovery和organic rankine cycle的经验，希望能多为journal和conference审审稿，
版上大侠们如果有对应领域的稿子需要reviewer，请与我联系（站内信或者zjnflyer@
gmail.com)，十分感谢。 |
|
m*******s
发帖数: 3142 | 36 【 以下文字转载自 Mathematics 讨论区 】
发信人: manifolds (流形), 信区: Mathematics
标 题: 问一个trace cycling invariance的问题
发信站: BBS 未名空间站 (Tue Mar 30 09:15:31 2010, 美东)
线性代数里头有个基本结论，对任意矩阵A和B，有tr(AB)=tr(BA)成立
量子力学的教科书也多处使用该性质，只不过把矩阵换成了算符，而且还推广到多个算
符的情况。
很多时候这个性质对解决问题很重要，可是也有明显不适用的时候。
比如很基本的commutator [x,p]=i\hbar,
展开就是xp-px=i\hbar,两边同时取trace，右边肯定不是0，从而说明tr(xp)≠tr(px)。
我的问题就是当A,B是算符的时候，tr(AB)=tr(BA)成立的充分条件是什么？
请高手帮忙。谢谢！ |
|
V*******n
发帖数: 534 | 37 The Berkeley researchers have interesting findings about psychological cycle for both women and men during the period of their midlife crisis.
Let's take a close look at what these researchers address in their research paper.
By age forty, women described as independent, bright and interesting in
adolescence were depressed, irritable and conflicted. However, by age fifty,these women had rebounded.At forty, the independent women were quite dissatisfied and in the midst of what looked like a crisi... 阅读全帖 |
|
d**s
发帖数: 920 | 38 In recent years, we have seen:
Real Estate goes up and burst
Commodities goees up and (may burst soon)
Stock went down from dot.com and had not recovered
I want to know which sector will do well next.
请推荐一本好的关于economy (or investment) cycle的书. |
|
w******e
发帖数: 493 | 39 【ID】
whatelse
【申请职务】
Cycling版版副
【理由】
喜欢骑车，愿意在版上讨论和大家共同进步（主要还是受教育）。也愿意当版副协助版
主工作，为大家服务。
【原则】
支持新版主，希望大家可以多讨论有关骑车的各种相关话题
【日期】
2008年6月17日 |
|
R*****s
发帖数: 41236 | 40 是否已阅读：1）《站规》2）《版务操作简易手册》?:
是
[申请ID]:
Rodimus
[申请版面]:
Cycling
[申请职务]:
版副
[版务经验]:
food版主
[申请纲领]:
尽力活跃版面气氛，共同提高
[版规草案]:
一切听版主的 |
|
i*********5
发帖数: 19210 | 41 是否已阅读：1）《站规》2）《版务操作简易手册》?:
是
[申请ID]:
ironman2015
[申请版面]:
Cycling
[申请职务]:
版主
[版务经验]:
三项全能版主
[申请纲领]:
推广自行车运动，自行车交通，自行车文化。
[版规草案]:
文明灌自行车水。 |
|
i*********5
发帖数: 19210 | 42 是否已阅读：1）《站规》2）《版务操作简易手册》?:
是
[申请ID]:
ironman2015
[申请版面]:
Cycling
[申请职务]:
版主
[版务经验]:
三项全能版主
[申请纲领]:
推广自行车运动，自行车交通，自行车文化。
[版规草案]:
文明灌自行车水。 |
|
p********r
发帖数: 3799 | 43 支持。
快批吧，cycling板，现在版主板斧都空着。 |
|
R*****s
发帖数: 41236 | 44 发现换老大了，跟随潮流重发一次...
是否已阅读：1）《站规》2）《版务操作简易手册》?:
是
[申请ID]:
Rodimus
[申请版面]:
Cycling
[申请职务]:
版副
[版务经验]:
food版主
[申请纲领]:
尽力活跃版面气氛，共同提高骑车水平
[版规草案]:
一切听版主的 |
|
a*********d
发帖数: 2763 | 45 是否已阅读：1）《站规》2）《版务操作简易手册》?:
1是
2是
[申请ID]:
againstwind
[申请版面]:
自行车运动(Cycling)版
[申请职务]:
班副
[版务经验]:
美加临床医学考版俱乐部(Pre_Resident_Club) 版副
[申请纲领]:
安装版主的指示，执行板斧职责。对网络秽语零容忍，营造和谐的股版讨论环境。
[版规草案]:
参见版主的竞选纲领。 |
|
i*********5
发帖数: 19210 | 46 版面:
Cycling
版务:
ironman2015
申请每日奖励M上限:
30 |
|
p********r
发帖数: 3799 | 47 代发版面/ID: 自行车运动(Cycling)
代发事由(主题标题或链接): 版面活动 （4月灌水包）
伪币金额: 300，下列ID，前十名每人两个包子（20伪币）；后十名每人一个包子（10
伪币）。
奖励/代发包子数量: 30
=====================
ironman2015
tianyu
heyihua
pecanlover
Rodimus
labiker
neverend
wolfstar76
penguinfly
zhanglaosan
===========================
yuwenchengdu
wuyan
Ktar
jekky
JerryMouse
yling
darkcc
flbufl
jasonz
cookiejar |
|
|
|
