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发帖数: 5204 | 2 RE 孵蛋的学弟/妹 giantbird05 (大鸟)
of course you can do it, just Store it in freezer.
pls refer:
Gelatin/Fibronectin
• Place 0.1g gelatin into a 500 ml glass bottle. Add 500 mL of dH20
and autoclave. The
concentration of gelatin is 0.02%. Fibronectin comes as a 5 mL liquid.
Dilute 1 mL of
fibronectin in 80 mL of 0.02% gelatin in a T-75 flask. Invert to mix.
Immediately
aliquot into 8 tubes of 10 mL. Store in freezer.
Coating Flasks
• All flasks must be coated with gelatin/fibronectin the ... 阅读全帖 |
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