w******e
发帖数: 1187 | 1 一年前刚看到这方面的paper就很惊艳，不过被做device的labmate打击了，说是虽然
看上去很美但操作难度极高。之后同是这个哥们时不时跟我说IVC的进展——sorting
越来越方便，droplet寿命越来越长。。。说不定很快我dream up的一些应用就能有人
做出来。当然不会是我——对laymen来说还是太难了：（ |
|
h**********r
发帖数: 671 | 2 兄弟我是个粗人，干发酵的。不知道这么多花花儿道道儿。瞎说说～
很多微生物或油料作物，会积累油脂形成lipid body，细胞膜表面上的lipid只是很少
一部分。
这些lipid body上面结合了不少蛋白。lipid body主要是甘油三酯（TAGs）啥的。不知
道对你有帮助没？
有兴趣可以翻翻下面的paper,更多的自己翻翻吧。
Protein composition of oil bodies from mature Brassica
napus seeds；
Synthesis, storage and degradation of neutral lipids in yeast；
The Gregarious Lipid Droplet. |
|
b****n
发帖数: 311 | 3 嘛蛋白能quantify lipid? lipid droplet上那些蛋白perilipin之类的恐怕不行吧，连
我都想的到的东西，不然人家早用了。而且我记得虫子的消化道那一圈可是有挺强的自
发荧光的，至少GFP channel如此。 |
|
w******e
发帖数: 1187 | 4 1.Microfluidic Droplet Technique for In Vitro Directed Evolution
Author(s): Wu N (Wu, Nan)1,2, Oakeshott J (Oakeshott, John)3, Brown S (Brown
, Sue)4, Easton C (Easton, Christopher)1, Zhu YG (Zhu, Yonggang)2
Source: AUSTRALIAN JOURNAL OF CHEMISTRY Volume: 63 Issue: 9 Pages
2. Ultrahigh Throughput Screening System for Directed Glucose Oxidase
Evolution
in Yeast Cells
Source: Combinatorial chemistry & high throughput screening (2011) volume
email: q************[email protected]
Thank y... 阅读全帖 |
|
w******e
发帖数: 1187 | 5 mRNA/ribosome/phage/bateria/yeast/microbeads/micro droplet display的铜锈们
都去什么会啊？
只知道有抗体相关的会，那做peptide/alternative scaffold/enzyme evolution
的有啥类似的会否？gordon系列有这个topic否？
多谢！ |
|
w******e
发帖数: 1187 | 6 让我对droplet/IVC的饭度大减呵呵。跟phage比也有definitive的优势——
毕竟是HT screening而不是selection。我小见识浅薄一惊一乍大家别见怪呵呵。
ms就wittrup一枝独秀？国内有phage/yeast display的牛人吗？
内行指点一下？ |
|
|
|
s**u
发帖数: 9035 | 9 Req Number: L11-177
Location(s): Boston MA
Career Field: Sales/Field Applications Scientist
Education: MS/PhD
Duties and Responsibilities:
Digital PCR Specialist Position
The successful candidate will utilize their knowledge and understanding of
digital PCR technologies to develop and support sales initiatives in the
United States. As part of the QuantaLife team in the Gene Expression
Division, this position will serve as the key liaison between the customer
and QuantaLife. The Specialis... 阅读全帖 |
|
|
g*********1
发帖数: 37 | 11 附件里面有用NIH droplet分析后图，更明显些 |
|
y**u
发帖数: 7459 | 12 too round and homogeneous in shape though, lipid droplets? considering that
it' s 3T3 |
|
l**********1
发帖数: 5204 | 13 microfluidic platforms plus nanopore
pls refer
1)
Choi JW et al. (2012)
High-Throughput Analysis of Protein–Protein Interactions in Picoliter-
Volume Droplets Using Fluorescence Polarization
Anal. Chem. 84: 3849–3854
http://pubs.acs.org/doi/abs/10.1021/ac300414g
2)
Rudenko MI et al. (2011)
Controlled gating and electrical detection of single 50S ribosomal subunits
through a solid-state nanopore in a microfluidic chip.
Biosens Bioelectron. 29: 34-9.
http://www.ncbi.nlm.nih.gov/pubmed/21855314
3)
... 阅读全帖 |
|
l**********1
发帖数: 5204 | 14 Continue:
Microfluidic tools for high-throughput applications
Microfluidic strategies might hold the key towards fast, simple and
inexpensive high-throughput screening platforms These platforms could
revolutionize basic research in biology and medicine, in addition to
dramatically reduce costs in key industrial areas such as drug discovery. To
this end the development of microfluidic tools, capable of dealing with
miniaturized samples (and therefore with large amounts of information) are
needed.... 阅读全帖 |
|
t*******e
发帖数: 119 | 15 目前大家关注的最多的和TAG有关的基因是DGAT，另外还有PDAT也较相关。此外还有和
lipid droplet形成有关的基因也比较受关注。 |
|
a******a
发帖数: 283 | 16 Wonder anybody has experience with it and what's the perspective? I only
know the one made by Bio-rad. It seems like there is another company (don't
remember the name) also claims they have this technology.
Any insight of any sort? |
|
s******s
发帖数: 13035 | 17 你是说raindance么？和454, ion torrent都是一牛发明的
t |
|
|
h******y
发帖数: 351 | 19 Raindance和Bio-Rad的ddPCR的原理是不一样的。
Raindance出来的结果不直观，而且不是绝对定量。
ddPCR定量的原理利用的是泊松分布，在低浓度范围内定量挺准，缺点是定量范围比较
窄。 |
|
w*****n
发帖数: 107 | 20 Two methods:
1) Streak the strain out on YPD plate (or selective plate), seal the plate,
and mail it in a pressure resistant envelope.
2) Grow the strain up in liquid culture (overnight is enough), put one
droplet (50-100ul) onto a small sterile waterman paper, wrap it with sterile
foil. Mail it in a regular envelope. |
|
t*d
发帖数: 1290 | 21 Science 21 June 2013:
Vol. 340 no. 6139 pp. 1459-1463
DOI: 10.1126/science.1229455
Report
H5N1 Hybrid Viruses Bearing 2009/H1N1 Virus Genes Transmit in Guinea Pigs by
Respiratory Droplet
Ying Zhang1,*,
Qianyi Zhang1,2,*,
Huihui Kong1,
Yongping Jiang1,
Yuwei Gao1,
Guohua Deng1,
Jianzhong Shi1,
Guobin Tian1,
Liling Liu1,
Jinxiong Liu1,
Yuntao Guan1,
Zhigao Bu1,
Hualan Chen1,2,†
- Author Affiliations
1State Key Laboratory of Veterina... 阅读全帖 |
|
z********8
发帖数: 818 | 22 下图绿色是Bodipy染的lipid droplet，红色是overexpressed protein。 请问大家这
个protein是在什么结构上？ 谢谢！ hela cells。 |
|
q**w
发帖数: 782 | 23 http://blog.sciencenet.cn/blog-404304-740945.html
南京大学的张辰宇教授是国内生命科学领域少有的、有自己独创概念的学者，其主要
的工作都集中在miRNA方面。关于miRNA的介绍很多，这里不做科普。10年的时候，张教
授课题组发表了两篇看起来没有什么关联的论文：
1. Identification and characterization of microRNAs in raw milk during
different periods of lactation, commercial fluid, and powdered milk products.
2. Secreted monocytic miR-150 enhances targeted endothelial cell migration.
这两篇文章都挺有意思，第一篇发在Cell Research上，讲的是从牛奶，不管是哺乳
期、商业化的牛奶，或者是奶粉里，都是可以鉴定出miRNA。第二篇发在Molecular
Cell上，讲的是miRNA能够被microvesicl... 阅读全帖 |
|
s******a
发帖数: 472 | 24 肝细胞细胞质有很强的自发荧光，主要来自lipid droplets或者vesicles。如果做
imaging的话，大家都有什么trick可以减少自发荧光。
我的感觉是在体外培养时间越长自发荧光也增强。 |
|
h*******o
发帖数: 4884 | 25 图上面蓝箭头的是lipid droplets
红箭头的，我觉得像是abnormal mitochondria， 又觉得像lysosome。 |
|
w********w
发帖数: 469 | 26 好像是airborne or droplet transmission
filter |
|
s***a
发帖数: 43 | 27 【 以下文字转载自 Faculty 讨论区 】
发信人: SWJSJ (BC), 信区: Faculty
标 题: 调研了一下清华最近回去的PI
发信站: BBS 未名空间站 (Mon Dec 15 16:27:25 2014, 美东)
云老师发了个贴说清华目前的找人标准，很多人不相信。特地调研了一下清华生命学院
最近回去的人的情况，如云老师所说，绝大多数都有2cns，如果只有一篇cns，那基本
上还有>2的子刊。这个不仅仅局限于胖老师经常说的结构生物学，而是所有方向都基本
如此。
当然这些新回去的绝大部分都是千青（有些不是的是因为回去那一年千青还没开始）。
网页上的Title有的是教授，有的是研究员，估计当时还没有统一。但校内一律都是副
教授，也就是相当于这边的AP。我还特地打听了一下，国内的TT制度好像没有Tenured
Associate这一档，清华5年考核通过了就是正经的full professor。
有些老师看不上国内的科研，我就仅列出两位回国后的publication，咱就不说颜宁等
这种Early T的个例了。说两个没太有名气的，还没有评上T的，各位老师可以参考一下
，... 阅读全帖 |
|
t*******o
发帖数: 516 | 28 我也是做结晶的。 你这种情况多半是溶液不对。所谓高盐是相对TEV酶切的。比如
500mM盐。 你得先保证你的蛋白稳定了才能谈其他的对不对。楼上说的加DTT估计也有
帮助。0.5mM TCEP还是弱了点。
再说了，谁说高一点的盐不能结晶的？最普通的做法就是，screening时，set up好
droplet后，密封前，在所有的well solution里补加等浓度的盐，比如500mM. |
|
A**H
发帖数: 4797 | 29 Current glucometers use test strips containing glucose oxidase, an enzyme
that reacts to glucose in the blood droplet, and an interface to an
electrode inside the meter,” explains Michael Strano, the Charles and Hilda
Roddey Associate Professor of Chemical Engineering at MIT. “When the strip
is inserted into the meter, the flux of the glucose reaction generates an
electrical signal,” he says. “The glucometer is calibrated so the number
appearing in its digital readout corresponds to the strength... 阅读全帖 |
|
C****c
发帖数: 1964 | 30 Can someone share these three papers with me? Thank you very much.
1. Nature Reviews Gastroenterology and Hepatology 10, 656-665 (November 2013
) | doi:10.1038/nrgastro.2013.183
NAFLD, NASH and liver cancer
2. Lipid droplets and liver disease: from basic biology to clinical
implications
Nature Reviews Gastroenterology & Hepatology 14, 343–355 (2017) doi:10.1038
/nrgastro.2017.32
3. Extracellular vesicles in liver disease and potential as biomarkers and
therapeutic targets
Nature Reviews Gastroen... 阅读全帖 |
|
b*****r
发帖数: 29 | 31 代朋友发帖
1. Research Assistantship (RA) for a PhD Student in Mechanical Engineering
An NSF-funded research assistant position is available for a new PhD student
in Mechanical Engineering at the State University of New York (SUNY) at
Binghamton. The research is focused on materials printing using a hybrid
inkjet-electrospray technique. The goal is to print highly-ordered
monolayers of functional nanoparticle inks at high throughput. The student’
s research will be experimental; however, close collab... 阅读全帖 |
|
b*****r
发帖数: 29 | 32 代朋友发帖
1. Research Assistantship (RA) for a PhD Student in Mechanical Engineering
An NSF-funded research assistant position is available for a new PhD student
in Mechanical Engineering at the State University of New York (SUNY) at
Binghamton. The research is focused on materials printing using a hybrid
inkjet-electrospray technique. The goal is to print highly-ordered
monolayers of functional nanoparticle inks at high throughput. The student’
s research will be experimental; however, close collab... 阅读全帖 |
|
m****o
发帖数: 310 | 33 I have a series contact angle measurement images (jpeg file), which contact
angle and droplet position changes a little from image to image. To avoid
error when analyze image by hand, is there any software (Image J or ...)
that can analyze the image automatically after I define the contact angle
for the first image? |
|
k****o
发帖数: 728 | 34 话说，他最近在ACS视频里提到自己最得意的两个工作，一是早年的那个正儿八经的著
名的有机反应，再一个就是90年代发在science上的疏水性渐变的monolayer能让water
droplet爬坡。从此他就开始其“simple" science之路了。 |
|
|
b*****r
发帖数: 29 | 36 代朋友发帖
1. Research Assistantship (RA) for a PhD Student in Mechanical Engineering
An NSF-funded research assistant position is available for a new PhD student
in Mechanical Engineering at the State University of New York (SUNY) at
Binghamton. The research is focused on materials printing using a hybrid
inkjet-electrospray technique. The goal is to print highly-ordered
monolayers of functional nanoparticle inks at high throughput. The student’
s research will be experimental; however, close collab... 阅读全帖 |
|
b**s
发帖数: 589 | 37 Today I show this paper to my advisor, but it seems that he has no
interest to it, So I decide to share it with you, my collegues, instead of
keeping it confidential :-P. I hope this paper will be useful. The main ideal
is
that droplets can coalesce into very stable strings or ribbons in sheared
polymer blend,
Enjoy it:
(K. Migler, Phys. Rev. Lett. 86, 1023, 2001.)
...potential microscale applications include conductive plastic wires,
ultrathin composite materials, and tissue engineerin |
|
w********h
发帖数: 12367 | 38 recommend one book:
Soft Materr Physics
(M. Daoud, C. E. Williams eds.)
The first chapter (Droplet: Cappilary and Wetting)
was written by F. Brochard-Wyart.
I just glanced over that,
he mentioned your spincoating problem.
I didn't go to details. you check that..
似
spin-coated |
|
w********h
发帖数: 12367 | 39 I think it is interdiffusion bah..
I never heard of negative diffusion coefficient...//expect more details.
Here are some papers, I don't know if they are helpful or not:
(1)
Spontaneous Double Phase Separation Induced by Rapid Hydrodynamic Coarsening
in Two-Dimensional Fluid Mixtures
Physical Review Letters Volume: 81, Issue: 2, July 13, 1998. pp. 389-392.
Tanaka, Hajime; Araki, Takeaki
(2)
New mechanisms of droplet coarsening in phase-separating fluid mixtures
The Journal of Chemical Physics V |
|
k*******n
发帖数: 2399 | 40 本人本身不是做高分子聚合的，只是在一个particle stablized emulsion体系里面用
了styrene作聚合单体。发现增加里面的particle浓度导致聚合速率降低，该particle
是7% 的SWNT负载在SiO2上。增加particle浓度从表观上是减小了聚合物颗粒的大小(~
um)。我看见不少emulsion polymerization都是增加particle浓度增加了反应速率，因
为emulsion droplet变小的话会导致反应体系compartamentalization降低bimolecular
termination的可能。我现在怀疑是不是该颗粒会quench聚合的radical，本人用AIBN
作为引发剂。该颗粒具有SWNT, COOH, Co, Mo和SiO2在表面上。本人提出另外一个可能
是emulsion drop变小，减少了monomer bimolecular reaction的速率。 那种更具有可
能性。先谢谢了。 |
|
t***u
发帖数: 20182 | 41 那这个就是pikering emulsion。我猜，假设所有initiator都在core里面，如果增加
particle浓度，droplet变小，但是数量会增多，总体来说core里面styrene对于
initiator的相对浓度降低，所以速度降低，这个反应用的多少度？AIBN的t1/2不一样
吧。 |
|
a****t
发帖数: 720 | 42 I am mostly interested in the dynamics of the droplet. So I will solve the
Navier-Stokes equation coupled with electrohydrodynamic force. Yes. I think
the electric field is static and is solved by Laplace equation. I did CFD
before. This research may require me couple the electric field effect into the NS momentum equation.
Are you working now?
electrical |
|
L********u
发帖数: 186 | 43 液滴燃烧显然是所有液体燃料燃烧的基础，这个领域在二三十年前很火，Law,
Sirignano等大牛做过系统的研究，超临界微重力环境下的也都做过。这个领域的研究
为液体燃烧提供了理论基础。
但液滴燃烧和喷雾理论结合的不是很好，显然喷雾是实际燃烧系统中肯定遇到的问题。
如果能把两者好好结合起来，用详细准确的液滴燃烧模型去优化喷雾燃烧模型，显然是
条出路。
NASA的项目，估计可能是微重力相关，或者推进系统。。。
这个领域出可以去各大内燃机，燃气轮机公司
留可以在学术界做做燃烧模拟
其实任何科研方向都是有出路的，只要自己不把路走死就好了。。。 |
|
f********t
发帖数: 32 | 44 呵呵，燃烧领域现在funding很少。而且作为international student没有绿卡很难找到
相关工作。即便找到，也不推荐。因为大部分项目都是和发动机，或者是NASA相关的。
到时候一不小心被FBI盯上就惨了。
还是看看有没有别的offer吧。 |
|
发帖数: 1 | 45 大家好, 本人机械工程PhD, 研究方向为 Microfluidics, Multiphase Flow,
Interfacial Phenomena, Synthetic Biology, Droplet Manipulation, Liposome,
Drug Delivery. 在本领域杂志(Lab on a Chip等)发过相关文章. 各位朋友如有合适的
审稿机会欢迎联系我: [email protected]/* */
多谢！ |
|
c******e
发帖数: 76 | 46 thanks for droplets explanation. so I did the right thing? reserve the slot
before they receive the letter? but how could I know if the letters are
scanned after arrival? since right now they are already shown in post office?
thanks |
|
a****9
发帖数: 773 | 47 hi,droplets,
many thanks for sharing the info. They are very useful.
Are u doing any externship or OB now? |
|
a****9
发帖数: 773 | 48 多谢 dok,droplets,fionaww 的指点
刀老师您不要生气，我也是不懂才来问的，我相信OB一定能受益匪浅。 |
|
l********y
发帖数: 2295 | 49 droplets, nn928: Thank you so much for your kind reply! I appreciate.
CS最忌讳的就是轻敌，不认真准备 -- Thanks so much for the advice. this is
really helpful, I definitely will go kaplan 5-day course before the real
exam! CS is not as easy as I expected, I thought it should be easy :-( |
|
t****o
发帖数: 342 | 50 My pleasure =)
Mr. Lith used to be a SP trainer in kaplan years ago, then he started this
workshop. He knows all the details about CS, and he is very helpful too.
Kaplan 5 day should be better, as droplet mm suggests. However, if time and
costs are concerns, this 2-day workshop is a good substitute. Save your "
vacation" days for later interview leave, which will take MANY days. |
|
