h**********r
发帖数: 671 | 1 我以前在国内时候提细菌基因组用的方法，貌似对酵母也work,我以前就提过一次酵母
，确实work的很好，还记得gel上的照片贼亮贼亮的。那时候年轻啊！问过别人，说
lysozyme对yeast也能work.
Extraction of DNA from Bacteria and Yeasts
(This protocol was developed by Housepainter on February 09, 2010)
1.Inoculate 5 ml of medium with a single colony of transformed bacteria or
yeasts.
2.Pour 1.0 ml of the culture into a tube. Centrifuge at maximum speed for 30
seconds at room temperature. Remove the supernatant.
3.Resuspend the bacterial pellet in 1.0 ml 0.85% NaCl by vigorous vortexi... 阅读全帖 |
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m***T
发帖数: 11058 | 2 楼上shakuras的几个贴子已经解释得挺清楚了。每个平台都有它的优缺点。Ion的优势是
simplicity, speed and scalability。说得通俗些，它的机器本身就是一台敏感度很
高的pH meter，核心技术其实都在chip里，所以它的运营成本会比较低。这些优点在
IVD方向都是比较重要的几个因素。
但它目前也有自己的缺点，比如throughput相对低，再有就是还需要emulsion PCR，都
是不利因素。好在目前整个公司对Ion项目的支持力度很大，有了invitrogen和ambion
的reagents，再加上AB这么多年在仪器方面的经验，所以Ion的前景应该还是不错的。
比如，Ion能在这么短的时间内发布OneTouch（自动化地emPCR），和整个公司对它的大
力支持是分不开的。 |
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l**********1
发帖数: 5204 | 3 Continue:
Microfluidic tools for high-throughput applications
Microfluidic strategies might hold the key towards fast, simple and
inexpensive high-throughput screening platforms These platforms could
revolutionize basic research in biology and medicine, in addition to
dramatically reduce costs in key industrial areas such as drug discovery. To
this end the development of microfluidic tools, capable of dealing with
miniaturized samples (and therefore with large amounts of information) are
needed.... 阅读全帖 |
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y******8
发帖数: 1764 | 4 透漏点Ion的新技术吧。
大家现在对Proton的error rate还有保留意见。不过如果真像传闻中的能把emulsion去
掉，应该能抢掉不少2500的市场。 |
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k***g
发帖数: 4904 | 5 随便查了几个网站发现卖的都好大罐，挺贵，我弄很少一点试试，从哪买比较方便啊？
这东西大家也有用自己的做的吗？我查了一下好像也挺好做的，温热一下离心再过滤一
下就行了好像？ |
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l****5
发帖数: 2968 | 6 包子酬谢
轻发g***********[email protected]
1) Bartolomé, B., Santos, M., Jiménez, J. J., Del Nozal, M. J., & Gómez-
Cordovés, C. (2002). Pentoses and hydroxycinnamic acids in brewer's spent
grain. Journal of Cereal Science, 36(1), 51-58.
2) Heinzelmann, K., & Franke, K. (1999). Using freezing and drying
techniques of emulsions for the microencapsulation of fish oil to improve
oxidation stability.Colloids and Surfaces B: Biointerfaces, 12(3), 223-229.
3) Gao, M. T., Hirata, M., Toorisaka, E., & Hano, T. (200... 阅读全帖 |
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Q**********r
发帖数: 214 | 7 谁能帮忙下载这篇文章
Nat Methods. 2006 Jul;3(7):545-50.
Amplification of complex gene libraries by emulsion PCR.
email:[email protected]
/* */
多谢了！ |
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z****y
发帖数: 613 | 8 I think, for the irregular particles --- use mechanical grinding;
for the regular particles --- use suspension or emulsion
polymerisaion; dispersion or precipitation polymerisation. |
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k*******n
发帖数: 2399 | 9 All the above discussions are so insightful, and many thanks for your
valuable imputs. Currently I am a postdoctoral researcher in Chemical
Engineering Department. Because of my project, I am working with some people
from petroleum engineering, focusing on the the enhanced oil recovery
utilizing emulsion techniques. Before I started my postdoc one year ago, I
got my Ph.D in physical chemistry from a U.S university. Along with the
cooperation with the PE guys, I have some interest on the reservio |
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m***c
发帖数: 637 | 10 招聘：中国联合利华 纳米材料 研究助理。欢迎海外留学人士。主要
针对上海
地区，但可以帮助寻找其它城市工作。请注明
要求。
If you are interested, please email your CV to d****[email protected]
with the
position you are applying as the title.
Assistant Research Scientist（纳米材料）
研究添加在乳液（护肤品）里的纳米材料
Job Responsibilities:
1.To design, prepare and characterise novel micro structured materials for
delivering benefits to consumers;
2.To design and prepare of novel colloidal systems, for example emulsion,
foam and dis |
|
c****8
发帖数: 283 | 11 现在想选导师读master，请大家帮忙参考下
一个研究方向如下：
Transport phenomena of multiphase systems and fluidization, computational
fluid dynamics (CFD), hydrogen storage, tire recycling, particle technology
in applications to coal gasification, production of gas from unconventional
gas reserves and hydrates, and energy and sustainability related issues.
有12项专利，现在的project在研究二氧化碳吸收和风力发电的涡轮研究
是个老教授，人很好
另一个的研究方向是
* Colloidal processing, including particle suspensions, foams and emulsions
* Wetting, spreading and adh... 阅读全帖 |
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s**n
发帖数: 1412 | 12 第一个.
technology
unconventional
emulsions |
|
P******x
发帖数: 259 | 13 1
technology
unconventional
emulsions |
|
v***n
发帖数: 123 | 14 The 2nd one.
To become that kind of member,he must be outstanding in his field, and he
should have good reputation in industry.
technology
unconventional
emulsions |
|
|
z**c
发帖数: 7595 | 16 是啊，我就需要谁给我点非常实用的乳化剂配方，乳化器设定及条件，不行我买个Ipad
2 送给他 ：） |
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y******1
发帖数: 730 | 17 乳化剂也分阴阳离子型，取决于你的体系。你可以找本精细化工配方的书，找关于涂料
的一些配方，会很有帮助。 |
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z**c
发帖数: 7595 | 18 刚才Google了一下中文，找到两个配方，有空试试，谢谢。 |
|
m***c
发帖数: 637 | 19 中国上海联合利华招聘以下化学，物理，材料，食物，生物海外学者。
Assistant Research Scientist--Deposition
Assistant Research Scientist-- Spectroscopy
Assistant Research Scientist--Food Science 助理研究专员
Assistant Research Scientist--Microbiology
Assistant Research Scientist--Polymer
Assistant Research Scientist-- Tea
Assistant Research Scientist--Skin
Assistant Research Scientist--Micro Materials-Functional Particulates Design
Assistant Research Specialist--Diabetes 研究专员-糖尿病
If you are interested, please send CV to D****[email protected] ... 阅读全帖 |
|
m***c
发帖数: 637 | 20 中国上海联合利华招聘以下化学，物理，材料，食物，生物海外学者。
本帖长期有效。
Assistant Research Scientist--Deposition
Assistant Research Scientist-- Spectroscopy
Assistant Research Scientist--Food Science 助理研究专员
Assistant Research Scientist--Microbiology
Assistant Research Scientist--Polymer
Assistant Research Scientist-- Tea
Assistant Research Scientist--Skin
Assistant Research Scientist--Micro Materials-Functional Particulates
Design
Assistant Research Specialist--Diabetes 研究专员-糖尿病
If you are interested, please send CV to DocSun@... 阅读全帖 |
|
m***c
发帖数: 637 | 21 招聘：世界500强上海研发中心诚聘以下物理化学生物医药海外留学人士。
If you are interested, please email your CV to d****[email protected] with the
position that you are applying as the title.
Assistant Research Scientist-Food Science
Assistant Research Scientist-Polymer
Assistant Research Scientist-Polymer
Assistant Research Scientist-Rheology in Deposition
Assistant Research Scientist-Water Purification
Assistant Research Scientist –Deposition
Product Development Technologist-Skin GDC护肤类产品开发研究(For Skin
Category)
Research Associate (Me... 阅读全帖 |
|
p*******n
发帖数: 2697 | 22 我的实验要用kerosene跟水混合的emulsion，而且需要大的油粒径，40microns左右吧
，但因为要过一个screw pump去加压，进系统。然而过了泵之后，油就小了，一直在
10micron左右。让我很头疼。什么表面活性剂什么的都没用。screw pump应该是对油粒
影响很小的，不知道peristaltic pump会不会好一点。
现在我想装个Coalescing Filter，让油粒从泵出来的时候能变大一点。
但在网上找了找，发现网上的Coalescing Filter都是除油的，没有找到我想要的让油
coalesce的。
比较抓狂，望牛人指点。
谢谢 |
|
i********t
发帖数: 1148 | 23 one more plz
Title: Study of emulsion polymerization of acrylates with a hyperbranched
polymer terminated by unsaturated double bonds
Author(s): Tang MF, Fan XD, Si QF, et al.
Source: ACTA POLYMERICA SINICA Issue: 7 Pages: 680-684 Published: JUL
2007 |
|
|
d********O
发帖数: 1196 | 25 Title: Grafting Reactions of (Hydroxyethyl)-cellulose During Emulsion
Polymerization of Vinyl Monomers
Chapter 18, pp 351–367
Chapter DOI: 10.1021/ba-1986-0213.ch018
Advances in Chemistry, Vol. 213
Thank you very much in advance! |
|
h*******o
发帖数: 1114 | 26 “Emulsion polymerization of Acrylic Monomers” Bull. CM-104 A/cf Rohm and
Haas Company, Philadelphia, PA |
|
k*******n
发帖数: 2399 | 27 你说的是什么体系？？我们老板是打一枪换个地方的，
Biofuel/emulsion还是CoMo/AlOH长碳管呢？？
后者的话做那个的阿根廷小姑娘跑到欧洲去做EXAFS了。。
估计想捣鼓点机理什么的出来。。 |
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S*****n
发帖数: 6055 | 28 显然emulsion啊。。。我对CoMo没兴趣。。。 |
|
d****i
发帖数: 360 | 29 Please help me! Thanks a lot! E-mail: x**********[email protected]
A new double emulsion solvent diffusion technique for encapsulating
hydrophilic molecules in PLGA nanoparticles
Show Abstract Cohen-Sela, E., Chorny, M., Koroukhov, N., Danenberg, H.D
., Golomb, G. 2009 Journal of Controlled Release 133 (2), pp. 90-95 |
|
d****i
发帖数: 360 | 30 Please help me! Thanks a lot! E-mail: x**********[email protected]
Freeze drying of double emulsions to prepare topotecan-entrapping liposomes
featuring controlled release
Wang, T., Wang, N., Li, T., Deng, Y. 2008 Drug Development and Industrial
Pharmacy 34 (4), pp. 427-433 |
|
d********O
发帖数: 1196 | 31 Titile: Grafting Reactions of (Hydroxyethyl)-cellulose During Emulsion
Polymerization of Vinyl Monomers
Advances in Chemistry, Vol. 213 Chapter 18, pp 351–367
Chapter DOI: 10.1021/ba-1986-0213.ch018
Please send to D********[email protected] , if this is more convenient for you.
Thanks and Have a great day! |
|
d********O
发帖数: 1196 | 32 Title: Degradation and grafting of hydroxyethyl cellulose during emulsion
polymerization
2004,124, 159-163
DOI 10.1007/b12161
Please send to D********[email protected] , if this is more convenient for you.
Thanks a lot! |
|
|
m***c
发帖数: 637 | 34 招聘：中国联合利华 纳米材料 研究助理。欢迎海外留学人士。主要
针对上海
地区，但可以帮助寻找其它城市工作。请注明
要求。
If you are interested, please email your CV to d****[email protected]
with the
position you are applying as the title.
Assistant Research Scientist（纳米材料）
研究添加在乳液（护肤品）里的纳米材料
Job Responsibilities:
1.To design, prepare and characterise novel micro structured materials for
delivering benefits to consumers;
2.To design and prepare of novel colloidal systems, for example emulsion,
foam and dis |
|
c*s
发帖数: 2145 | 35 【 以下文字转载自 Macromolecules 讨论区 】
发信人: cds (我去哪哪衰退), 信区: Macromolecules
标 题: 帮忙找个文章把， 在JOURNAL OF COATINGS TECHNOLOGY 上
发信站: BBS 未名空间站 (Thu Jan 20 01:07:22 2011, 美东)
Epoxy silanes in reactive polymer emulsions
Author(s): Chen MJ, Osterholtz FD, Chaves A, et al.
Source: JOURNAL OF COATINGS TECHNOLOGY Volume: 69 Issue: 875 Pages: 49
-55 Published: DEC 1997 |
|
|
J********i
发帖数: 50662 | 37 我试过测zeta电位
可能因为是油相体系，zeta电位很奇怪，一会儿正，一会儿负
测zeta电位，然后找公式算 |
|
|
J********i
发帖数: 50662 | 39 哦，那我再去试试
可能粒子的尺寸分布有关，尺寸太分散就不好弄 |
|
m****o
发帖数: 310 | 40 Binks的文章大都关于emulsion stabilization。请问f0008有没有更具体的建议？ |
|
|
z**c
发帖数: 7595 | 42 果然没人肯帮我 ：） 但愿我的Formula Works，能卖300万刀一年。 |
|
m***c
发帖数: 637 | 43 中国上海联合利华招聘以下化学，物理，材料，食物，生物海外学者。
Assistant Research Scientist--Deposition
Assistant Research Scientist-- Spectroscopy
Assistant Research Scientist--Food Science 助理研究专员
Assistant Research Scientist--Microbiology
Assistant Research Scientist--Polymer
Assistant Research Scientist-- Tea
Assistant Research Scientist--Skin
Assistant Research Scientist--Micro Materials-Functional Particulates Design
Assistant Research Specialist--Diabetes 研究专员-糖尿病
If you are interested, please send CV to D****[email protected] ... 阅读全帖 |
|
R****k
发帖数: 234 | 44 you can try dried vinyl acetate-ethylene copolymer. it is made by emulsion
polymerization. particle size is hundreds of nm. |
|
m***c
发帖数: 637 | 45 中国上海联合利华招聘以下化学，物理，材料，食物，生物海外学者。
Assistant Research Scientist--Deposition
Assistant Research Scientist-- Spectroscopy
Assistant Research Scientist--Food Science 助理研究专员
Assistant Research Scientist--Microbiology
Assistant Research Scientist--Polymer
Assistant Research Scientist-- Tea
Assistant Research Scientist--Skin
Assistant Research Scientist--Micro Materials-Functional Particulates
Design
Assistant Research Specialist--Diabetes 研究专员-糖尿病
If you are interested, please send CV to D****[email protected] ... 阅读全帖 |
|
d*****n
发帖数: 196 | 46 Research progress on Pickering emulsions.
Huaxue Jinzhan (2009), 21, (7/8), 1418-1426.
Thanks! |
|
m***c
发帖数: 637 | 47 再转几个中国上海联合利华内部推荐招聘的位置给大家，尽量找联系人内部推荐。
中国上海联合利华内部推荐，招聘。
If you are interested, please email your CV to d****[email protected] with the
position that you are applying as the title.
Assistant Research Scientist-Polymer
Assistant Research Scientist-Rheology in Deposition
Assistant Research Scientist-Water Purification
Assistant Research Scientist –Deposition
Assistant Research Scientist-Polymer
Work in the Discover in R&D function for the research in Polymer Science
area and perform as an active project/team... 阅读全帖 |
|
|
a*****e
发帖数: 4936 | 49 汽车(automotive)
消泡剂(antifoam)
农业(Agricultural)
烷基芳基硅氧基硅油(Alkylaryl siloxane)
汽车保养(Auto care)
上光剂(Polish formulation)
后处理(Post-finishing)
脱模(Release)
工业脱模剂(Industrial Release Agents)
再喷涂(Paintable)
表面活性剂(Surfactant)
上面的汉语对不对？
==================
car
boat
gum
rain
antifoam
solvent
emulsion
siloxane
alkylaryl
surfactants
polyether
对应的通用的汉语是什么？
多谢！ |
|
S****D
发帖数: 5 | 50 Job opportunity from Unilever China R&D (联合利华中国研发中心)
Research Specialist - Shanghai
Unilever China R&D Discover Shanghai is currently recruiting a Research
Specialist in Colloidal Microstructure team. The job description details are
as following. You are welcome to login Unilever website for job application
(www.unilever.com.cn)
Job Responsibilities:
1.To play a key role in developing skills, building and maintaining
capability in the specific area, and contributing to the skill base in
general;... 阅读全帖 |
|
