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全部话题 - 话题: enhance
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T*****e
发帖数: 247
1
首先,这是个问题。
其次,我列举几种我知道的方法,并稍微评价优缺点。
最后,希望大家补充其它方法。目的是集思广益。
1.找到调控序列(Regulatory elements)
这个可以做promoter bashing,一段一段去掉调控序列中的某一部分。根据剩余部分是
否仍然有表达或者对某个刺激有反应,这样可以推测出Enhancer中哪一段序列是必须的
。这个运气好的话,应该能找出一小段必要的序列。我看有人能找到大概500bp就可以
了,有人能缩小到100bp,还有牛人能缩小到20bp。好吧,那基本离最后的调节序列的
大小不远了。
Pros:思路相对简单,结果相对清楚
Cons:如果调控序列有几个,并且分布不集中,又或者有几个不同的转录因子参与,于
是乎有不同的调控序列且分布分散,那么,很难有干净容易解释的结果。
2.做reporter盲筛
有了Enhancer当然很容易做reporter,从早年的CAT到现在随处可见的luciferase或者
GFP之类的,reporter用的相当普遍。有了reporter想要找转录因子,最简单的就是挨
个试了。过表达或者KD都行。
Pros:rep... 阅读全帖

发帖数: 1
2
来自主题: Biology版 - questions for CMV enhancer
印象中大部分的载体上的CMV启动子组合都是CMV enhancer+CMV promoter,比如pEGFP-
C1载体。
最近实验室从SBI买了一个载体pCT-Tubulin-GFP,看了公司给我的map,我也测序验证
了下,看到这个载体上的启动子只有CMV promoter,没有enhancer。
这个载体使用以及基因表达完全没有问题。我就是突然好奇想向各位老师请教下这个
CMV enhancer是否是必须的?理论上CMV enhancer肯定是能增强CMV promoter的效率。
但是如果没有的话例如SBI这个载体用起来也没有啥问题,表达量也是刚刚的。哪位老
师可以给讲解下CMV enhancer的功能吗?有没有谁比较过with enhancer和without
enhancer的CMV promoter的基因表达效率? 谢谢啦!
l****z
发帖数: 29846
3
By Fred Lucas
November 1, 2011
(CNSNews.com) – While six-year-old girls and retired school teachers with
bladder cancer were subjected to intrusive pat-downs by Transportation
Security Administration officials at U.S. airports after Umar Farouk
Abdulmutallab tried to detonate his underwear on a Northwest Airlines flight
on Christmas 2009, officials from Sudan--one of just four countries the
State Department lists as a state sponsor of terrorism--were “exempted from
enhanced screening” at airport... 阅读全帖
w*******y
发帖数: 60932
4
The Witcher EE Directors Cut $5 on D2D and IGN PC Store.
Link:
http://www.direct2drive.com/6763/product/Buy-The-Witcher:-Enhan
#39;s-Cut-Download" rel="nofollow" target="_blank" onclick="_gaq.push(['_
trackEvent', 'thread', 'click', '2818321 - the-witcher-enhanced-edition-5-
d2d-and-ignpc-store-75-off']);">http://www.direct2drive.com/6763/...t-Download
Link:
http://pcstore.ign.com/6763/product/Buy-The-Witcher:-Enhanced-E
#39;s-Cut-Download" rel="nofollow" target="_blank" onclick="_gaq.push(['_
t... 阅读全帖
w*******y
发帖数: 60932
5
The Fort by Bernard Cornwell is a free ebook optimized for iPad, iPhone,
iPod Touch & NookColor owners. It's enhanced with audio & video, originally
priced at $25.99 and it's a novel about an important battle in the American
Revolutionary War. Here is the description:
"While the major fighting of the war moves to the south in the summer of
1779, a British force of fewer than a thousand Scottish infantry, backed by
three sloops-of-war, sails to the desolate and fog-bound coast of New
England. Est... 阅读全帖
l******7
发帖数: 2234
6
来自主题: Automobile版 - 说说enhanced driver license
很不幸,因为所谓的Real ID ACT,我所在州已经从2014年就开始实行enhanced driver
license/ID. 就是在驾照上面新增了2个地方:enhanced和美国国旗(表明国籍)。
我们没有入籍的,是不是不能申请enhanced driver license ?普通驾照已经不能作为
真实有效的ID,来出入境和飞机,以及进入联邦机构场所。
大家怎么看?都去申请了增强版驾照吗?
c**0
发帖数: 9331
7
来自主题: FleaMarket版 - [求购]QB Pro w/Enhanced Payroll 2010 $154
我想要的物品:
QB Pro w/Enhanced Payroll 2010 $154
单张面值:
QB Pro w/Enhanced Payroll 2010 $154
可接受的价格(必须明码标价!):
QB Pro w/Enhanced Payroll 2010 $154
my label, no fee paypal
物品新旧要求:
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
其他补充说明:
广告的有效期:
物品来源:
我的联系方式:
二手交易风险自负!请自行验证是否合法和一手卡!:
e**********0
发帖数: 7133
8
来自主题: FleaMarket版 - [求购] QB Pro w/Enhanced Payroll 2010 $155
我想要的物品:
QB Pro w/Enhanced Payroll 2010 $155
单张面值:
QB Pro w/Enhanced Payroll 2010 $155
可接受的价格(必须明码标价!):
QB Pro w/Enhanced Payroll 2010 $155
my label, no fee paypal
物品新旧要求:
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
其他补充说明:
广告的有效期:
物品来源:
我的联系方式:
二手交易风险自负!请自行验证是否合法和一手卡!:
a********1
发帖数: 96
9
突然收到 Enhanced Collection Company, LLC 这家公司的讨债信。 很吃惊。
讨债人是 Enhanced Collection Company, 他们是帮ATT 找我讨债。 我后来和ATT 确
认过, 他们是把我的帐算错了。 我早就关掉ATT的帐了。 最后ATT 把我的帐户正式关
掉, 然后把我的帐户清零。 但是我不确定要不要回复那个Enhanced Collection
Company, LLC 公司。
现在还没有看我的credit report, 但是不知道为什么讨债公司会有我的新的地址?
不知道朋友解决过这个问题,能不能帮我一个忙, 告诉我一下要怎么解决这个无缘无
故的讨债公司?
我记得, 如果我无视这个帐目的话, 过一段时间, 他们是有权利起诉我的。 我必须
得把这个弄掉。
先在这里谢谢了。
希望有高人可以指点一下。
c******k
发帖数: 1089
10
Dear Stakeholder,
On March 19, 2015, U.S. Citizenship and Immigration Services (USCIS) hosted
a listening session on business-focused immigration enhancements. Such
enhancements are part of the November 2014 executive actions to modernize,
improve, and clarify employment-based immigrant and nonimmigrant visa
programs to grow our economy and create jobs.
We continue to encourage stakeholders to submit comments to Public.
[email protected]
/* */ in response to the specific questions that USC... 阅读全帖
w****g
发帖数: 597
11
来自主题: Internet版 - Skype positions itself as 'enhancement'
Skype positions itself as 'enhancement' VoIP
W. Gardner
TechWeb News
(05/24/2005 3:33 PM EDT)
With three million users in the U.S. and a rapidly-growing group of partners
in its new affiliates program, Skype Technologies is facing a unique challenge
presented by the FCC's order that VoIP providers must provide access to
emergency 911 numbers.
The Europe-based firm is positioning itself as an "enhancement" service to
existing telephone service and not as a "replacement" service. "We're an
enhance
b******n
发帖数: 80
12
Sorry I can not type it in chinese since I am in lab right now.
There are lots of papers concerning the hybrid structure in localized
surface plasmon resonance (LSPR). I found that they are 2 catalogs. One is
photo quenching, the other is PL enhanced.
I have asked a expert that whether the PL of semiconductor can be enhanced
by LSPR. He said it is definitely not because the excited electron-hole pair
will transfer to metal rapidly resulting in quenching.
In my opinion, that is just one factor. I... 阅读全帖
w*******y
发帖数: 60932
13
来自主题: _DealGroup版 - 【$】Booty Enhancement $8.95!
BOOTY ENHANCEMENT Spell Cast by Powerful Wiccan Witch!
Only $8.95!
Link!:
http://cgi.ebay.com/BOOTY-ENHANCEMENT-Spell-Cast-Powerful-Wiccan-Witch-/220496575102?pt=LH_DefaultDomain_0#ht_6847wt_1165
#ht_6847wt_1165" target="_blank">Link! [Link!:
http://cgi.ebay.com/BOOTY-ENHANCEMENT-Spell-Cast-Powerful-Wiccan-Witch-/220496575102?pt=LH_DefaultDomain_0#ht_6847wt_1165
#ht_6847wt_1165" target="_blank">ebay.com]
x******h
发帖数: 13678
14
Optomec Awarded Air Force Contract to Enhance Fuel Cell Manufacturing System
Upgrades Target High Volume Production of Solid Oxide Fuel Cells
ALBUQUERQUE, N.M.--(BUSINESS WIRE)--Optomec announced today that it has
received a new contract from the Air Force Research Laboratory (AFRL) to
deliver high throughput enhancements to its Aerosol Jet® system. The
upgrades will be made to a system that is being used by AFRL to develop and
prototype high efficiency solid oxide fuel cells (SOFCs) based
K*******s
发帖数: 105
15
一个recruiter 联系我,可惜感觉背景不是很mach,又让我给他推荐,看看谁有兴趣,
Senior Reservoir Simulation--Enhanced Recovery Engineer
Job Responsibilities Include:
1. Conducting and directing integrated reservoir studies employing
compositional
simulation to assist in the design and management of client’s CO2 floods.
2. Coordinating and directing fluid and rock property acquisition
programs.
3. Coordinating and directing integrated reservoir description programs
employing
advanced geostatistical procedures designed... 阅读全帖
C*******d
发帖数: 1553
16
柜员谎称没有学生checking
骗我们开了一个每月平均balance 5000刀才免15刀月费的enhanced checking
我们穷学生搞个毛线的enhanced
现在想把这账户转成学生账户,并且要投诉柜员
有什么法子么?
e******e
发帖数: 10121
17
来自主题: Money版 - AMEX Enhanced Biz Gold
昨天登录进去看到说有OFFER,给我UPGRADE到ENHANCED BIZ GOLD,享有一些优惠另外
再给15000点(忘了需要花多少钱)。提到说UPGRADE到ENHANCED BIZ GOLD后需要每年
交175.
奇怪,BIZ GOLD不是一直都每年CHARGE 175么?那还有什么区别?
有人也遇到么?
l*********d
发帖数: 73
18
【 以下文字转载自 Immigration 讨论区 】
发信人: lookforward (dadu), 信区: Immigration
标 题: will working for FDA enhance the chance to get EB1A approved?
发信站: BBS 未名空间站 (Fri Aug 12 15:30:33 2011, 美东)
I am wondering if working for FDA as a postdoc can enhance the chance for
EB1A, comparing to the post-doc jobs at other universities.
If I have another offer with a small non-profit lab that will sponsor me
EB1B, will this offer be better than FDA offer in terms of green card
application?
y***e
发帖数: 6082
19
在啥地方工作跟1A基本没啥关系,1B也就出示个在职证明罢了

【 以下文字转载自 Immigration 讨论区 】
发信人: lookforward (dadu), 信区: Immigration
标 题: will working for FDA enhance the chance to get EB1A approved?
发信站: BBS 未名空间站 (Fri Aug 12 15:30:33 2011, 美东)
I am wondering if working for FDA as a postdoc can enhance the chance for
EB1A, comparing to the post-doc jobs at other universities.
If I have another offer with a small non-profit lab that will sponsor me
EB1B, will this offer be better than FDA offer in terms of green card
applicati... 阅读全帖
d*i
发帖数: 9453
20
☆─────────────────────────────────────☆
mark (花生,微爷远爷的爸爸) 于 (Sun Sep 9 09:11:56 2007) 提到:
These results indicate that repeated bouts of exercise cause enhanced fat
metabolism compared with a single bout of prolonged exercise of equivalent
total exercise duration.
http://jap.physiology.org/cgi/content/abstract/102/6/2158?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=Goto&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT
Enhancement of fat metabolism by repeated bouts of moder
s*********r
发帖数: 4210
21
来自主题: GunsAndGears版 - 第一把枪 Sig P226 Enhanced Elite
enhanced elite 这个系列最明显的特点之一就是这个变小的握把. 这个握把也是最先
出现在enhance elite的型号上, 所以这个握把就俗称e2握把了. 现在这种握把已经普
及的整个P229/6的产品线上了.
b*****e
发帖数: 5133
22
来自主题: GunsAndGears版 - 第一把枪 Sig P226 Enhanced Elite
E2 = Enhanced Ergonomics
Enhanced Elite比普通E2晚上市半年 :)
w*******y
发帖数: 60932
23
Newegg
For those that missed the $30 deal:
Mushkin Enhanced Silverline 4GB (2 x 2GB) 240-Pin DDR3 SDRAM DDR3 1333 (PC3
10666) Desktop Memory Model:
http://www.newegg.com/Product/Product.aspx?Item=N82E16820226092
$53.99 - $20 rebate:
http://images10.newegg.com/uploadfilesfornewegg/rebate/SH/Mushkin5mirsNov26Nov2910mc69a.pdf

= $33.99 with free shipping
There's also a single 4GB module:
Mushkin Enhanced Silverline 4GB 240-Pin DDR3 SDRAM DDR3 1333 (PC3 10666)
Desktop Memory:
http://www.newegg.com/... 阅读全帖
w*******y
发帖数: 60932
24
GoG.com is having a 50% off sale for Witcher: Enhanced Edition Director's
Cut digital download. It's $4.99, a bit cheaper than during the Steam
Winter Sale, and without any DRM.
Link:
http://www.gog.com/en/gamecard/the_witcher
What's cool about it:
* Arguably one of the best RPGs ever made, with a mature, non-linear
story and a challenging combat system
* Beautiful graphics and an enchanting soundtrack create a very unique
dark fantasy atmosphere
* The Witcher's universe is created ... 阅读全帖
w*******y
发帖数: 60932
25
Part of their holiday daily sale, GamersGate has The Witcher Enhanced
Edition:
http://www.gamersgate.com/DD-WITCHEREEDC/the-witcher-enhanced-e
for $2.50.
**Probably does not activate on Steam.**
Link to all past daily deals they've had so far: http:// 43046#post45443046
w*******y
发帖数: 60932
w*******y
发帖数: 60932
27
Toys 'R' Us:
http://www.toysrus.com/product/index.jsp?productId=12644778
currently has The Witcher 2: Assassins of Kings Enhanced Edition (Xbox) on
Pre-Order for $44.99. Shipping is free.
Estimated Ship date: 04/17/2012
Enhanced Edition Content:
Game DVDs The Witcher 2: Assassins of Kings game on 2 DVDs.
OST CD: Audio-CD with in-game music.
World Map: A map of the game's world.
Quest Handbook - The Witcher 2 quest handbook for both novice and
experienced role-playing fans.
Game Manuel: A ruleb... 阅读全帖
m***y
发帖数: 79
28
要买车了,dealer email说
this unit has the following dealer added enhancements: First Place Finish (
Exterior/Interior Protectant w/3-Yr Warranty),Pin Stripes, and Nitrogen
Fill in tires, all a $694 value. 他家给的价最低,尽管加了这些东西。
我就想问问大家,这些多加的应该没有什么问题吧,因为不是厂家自己的option,是
dealer 自己加上的。
想起来以前发生的事情,dealer 卖车赠送了不是厂家的脚垫,然后刹车就出问题了,
刹不住。
O*********h
发帖数: 4674
29
就买了跟cable,$18
程序网上都有,只要确定你的车里有combox就可以了,就是trunk后面的垫子全部揭开
,看到有个铝盒子,上面写着combox就可以了
具体bimmerfest上都有
2015 enhanced bluetooth和usb都是328i标配了
2014年以及以前美国大多数车应该都有combox,因为美国的标配有bmw asistance, e-
call,这个就要求有gps,然后bmw干脆就都配了telematics combox
刷了之后,可以蓝牙streaming,usb接iphone后可以看短信,有专辑图片,还有很多很
多其他小功能
15年之前这个option 卖600,但是其实就是软件不同而已
t******k
发帖数: 553
30
来自主题: Automobile版 - 说说enhanced driver license
必须是美国公民。再说了,既然都是公民了,国际飞行还是护照最安全。如果去加拿大
墨西哥加勒比,美国人还是老大,带passport card就可以了。所以总体来讲,这个
enhanced driver license,是很傻逼的一个政策。
M*****i
发帖数: 1242
31
想卖的物品:
2 QuickBooks Pro 2012 W/QB Enhanced Payroll 2012 @180 each
单张面值:
可接受的价格(必须明码标价!):
see above
物品新旧要求:
first hand and legal
邮寄方式要求:
YL
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
BOA, bill pay
chase quickpay
其他补充说明:
广告的有效期:
till gone
物品来源:
我的联系方式:
prefer m************[email protected](please refer your ID in the email, thanks)
or pm (will have several hours delay.)
j*****e
发帖数: 413
32
来自主题: ebiz版 - paypal的enhanced Account
今天被通知变成enhanced Account了,好事还是坏事?
E*******r
发帖数: 2710
33
求一份280刀的QB Enhanced Payroll Rebate From的copy
staples网站上找不到了
rebate offer #13-51243
email: eadreamer@gmail DOT com
a***d
发帖数: 11
34
学校宣布了budget cut, 目前是cut some benefits. 但有进一步措施的可能. 很担心
学校会砍掉university research enhancement grant, 目前它包含了对Postdoc, Phd
student的经济资助, 教授是否应该提前一段时间告诉他们让他们也有点准备?
o****9
发帖数: 479
35
I just have been told during departmental meetings and email announcement
that there are enhanced security checks on campus. The things that are
related to a regular faculty/staff/student are that:
1) If a person will make a presentation in a conference held in a foreign
country, then s/he needs to schedule a meeting with a campus security czar
to show the presentation materials, like slides. A trip can only be allowed
upon getting approved by the security czar.
2) If a university owned laptop i... 阅读全帖
I*****k
发帖数: 550
36
我想要的物品:
QB Pro w/Enhanced Payroll 1-3 Employees 2010
单张面值:
可接受的价格(必须明码标价!):
130
物品新旧要求:
new, intact, not crushed
邮寄方式要求:
my fedex label
买卖双方谁承担邮寄损失(Required if not code only):
default
付款方式说明:
paypal or boa
其他补充说明:
need a box outside it to ship
广告的有效期:
物品来源:
staples
我的联系方式:
mitbbs
二手交易风险自负!请自行验证是否合法和一手卡!:
k*****i
发帖数: 2373
37
我想要的物品:
QuickBooks Pro w/Enhanced Payroll 160+ML
单张面值:
可接受的价格(必须明码标价!):
物品新旧要求:
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
其他补充说明:
广告的有效期:
物品来源:
我的联系方式:
二手交易风险自负!请自行验证是否合法和一手卡!:
M*****i
发帖数: 1242
38
想卖的物品:
2 QuickBooks Pro 2012 W/QB Enhanced Payroll 2012 @180 each
单张面值:
可接受的价格(必须明码标价!):
see above
物品新旧要求:
first hand and legal
邮寄方式要求:
YL
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
BOA, bill pay
chase quickpay
其他补充说明:
广告的有效期:
till gone
物品来源:
我的联系方式:
prefer m************[email protected](please refer your ID in the email, thanks)
or pm (will have several hours delay.)
D******e
发帖数: 207
39
想卖的物品:
QuickBooks Enhanced Payroll 2012 @$100
单张面值:
$229
可接受的价格(必须明码标价!):
$100
物品新旧要求:
first hand and legal
邮寄方式要求:
包邮
买卖双方谁承担邮寄损失(before me after you):
付款方式说明:
paypal no cc
其他补充说明:
广告的有效期:
till gone
物品来源:
STAPLES
我的联系方式:
PM
b2
发帖数: 427
40
enhanced policy covers more things like access,building permit ,zoning,
supplement tax,surface extraction,Encroachment. --
h******g
发帖数: 96
41
买owner title insurance一般是买basic得还是enhanced得?
I****g
发帖数: 102
42
看lz对房子的价值怎么估计了,这个title insurance本来就是个保险,等你房子的
ownership出现问题再拿来用的。enhanced就是将来房子要是涨价了,那么你的赔款相
应的也会随着房价上升,但是basic的话,你买价是多少就是多少,就算房子涨价了也
不会改变。
p********x
发帖数: 5583
43
有好几个enhanced coverages
1. Amendment of exception on Area and Boundaries
2. Restrictions, Encroachments, Minerals Endorsement
3. Minerals and Surface Damage Endorsement
这都是保什么的?
a***y
发帖数: 50
44
小弟是BOA的账户,可能是前一段从saving账户里转出操作太频繁了。。。
收到了BOA寄来的信说,要把Saving账户强行转成Enhanced Checking账户。。
不知道有什么坏处和影响呢。。请版友指点,非常感激!!
谢谢!
l*********u
发帖数: 27
45
大家好,
我的美国银行debit card已经使用一年
最近突然发现被收了monthly fee for bofa enhanced,一笔是15刀
再往前一查差点儿气的吐血:已经陆续被收了五次这个费用
google后发现这简直是霸王条款:只有每个月存2000或保持余额在5000以上或刷美国银
行绑定的信用卡才能免除这个费用
问题是我的工资三个月发一次(CSC公派留学生,一个月1700);两个人过日子,也不
可能总是保持余额在5000以上;而且一直申请不上任何信用卡,包括他们自己家的
想想我的肺都快气炸了,这一年已经给这个该死的银行贡献了75刀!
请问有什么办法能免除这个费用吗?
btw,昨天去柜台申请了一次,终于申请上了他们的信用卡,看来以后可以不用交这个钱
了,但当时问店员,得到的答复是以前收过的费用肯定要不回来了
不知道还又没有什么补救的方法?
谢谢!
p******a
发帖数: 1170
46
另外关于你说的“霸王条款”:人家提供了ebanking这个免费的checking option你不
用,非要用enhanced,达不到免月费要求还说人家霸王……实在是有点无语
b**n
发帖数: 54
47
我也是enhanced account, 开户时银行应该和你说清楚的吧,怎么免月费。
现在你可以和他们商量一下,能不能退,我觉得至少最近的一个月的能退。然后转成
echecking,什么费都没有,如果你不去柜台的话。
l*********u
发帖数: 27
48
感谢大家的回复
我自己确实也太粗心了
有一次好像看到这个条款,以为是自己买车时柜台取现才收的钱
还有一个问题:既然echecking不收费,那这个enhanced有什么优点?
不知道我这么长时间有没有take advantage,如果有的话,也就算了
p******a
发帖数: 1170
49
其实不能用柜台这一项很伤的啊~ATM每天有限额的。想大额取现的时候只有去柜台。要
么就开现金支票10刀一张。
Enhanced可以开2个checking,加一个MM Savings。而且送一本支票。
其实你不怕麻烦的话……从柜台取2000,然后ATM存进去就能免月费了……BoA没说是DD
,所以只要是从外面来了2000就行
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