s**u
发帖数: 9035 | 1 我准备测定病人Sputum的Non-neuronal Enolase,
Which Company ELISA kit is better?
Thanks for sharing. |
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s**u
发帖数: 9035 | 2 我准备测定病人Sputum的Non-neuronal Enolase,
Which Company ELISA kit is better?
Thanks for sharing. |
|
s**u
发帖数: 9035 | 3 我准备测定病人Sputum的Non-neuronal Enolase,
Which Company ELISA kit is better?
Thanks for sharing. |
|
s**u
发帖数: 9035 | 4 我准备测定病人Sputum的Non-neuronal Enolase,
Which Company ELISA kit is better?
Thanks for sharing. |
|
b********2
发帖数: 148 | 5 Thank you very much for the reply.
可不可以说说还有哪些可能性？
除了CT和增强CT，还做一些肿瘤指标的血液检查，包括癌胚抗原（carcinoembryonic
antigen
），细胞角蛋白19片段，神经元特异性烯醇化酶(neuron-specific enolase，NSE)，结
果都比较靠近或略微高于参考值。医生说由此可以排除肿瘤的可能性。还做了血沉（
ESR）和常规血相，其中血沉略高，医生说有此可以排除血液方面的问题。你怎么看? |
|
b********2
发帖数: 148 | 6 Thank you very much for the reply.
可不可以说说还有哪些可能性？
除了CT和增强CT，还做一些肿瘤指标的血液检查，包括癌胚抗原（carcinoembryonic
antigen
），细胞角蛋白19片段，神经元特异性烯醇化酶(neuron-specific enolase，NSE)，结
果都比较靠近或略微高于参考值。医生说由此可以排除肿瘤的可能性。还做了血沉（
ESR）和常规血相，其中血沉略高，医生说有此可以排除血液方面的问题。你怎么看? |
|
m*******h
发帖数: 896 | 7 If you want to try top-down. You need definitely two things:
1) A proper fragment method for break down large molecule. low energy
CID can handle some small proteins, but I think Enolase is too big
for low energy CID. High energy CID, ECD may help. But the fragmentation
mechanism are quite different from low engergy CID.
2) you need a high accuracy in mass determination. some time, 100ppm
error will give you so many possible matches.
It is not necessary that the weakest bond be broken first |
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b****u
发帖数: 903 | 8 Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):651-7. Epub 2008 May
14.
Structure of human alpha-enolase (hENO1), a multifunctional glycolytic
enzyme.
many thanks.
please send to: a******[email protected] |
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