s**********y
发帖数: 122 | 1 1.Improved efficiency of bovine somatic cell nuclear transfer by optimizing
operational procedures.
Zhao LW, Yang XY, Guan PF, Fu J, Li H, Zhou YY, Huang SZ, Zeng YT, Zeng FY.
J Reprod Dev. 2009 Oct;55(5):542-6. Epub 2009 Jul 1.
PMID: 19571467 [PubMed - indexed for MEDLINE]Free Article
Related citations
2.Selection of in vitro produced, transgenic embryos by nested PCR for
efficient production of transgenic goats.
Huang SZ, Huang Y, Chen MJ, Zeng FY, Ren ZR, Zeng YT.
Theriogenology. 2001 Sep 1;5... 阅读全帖 |
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R****0
发帖数: 138 | 2 β-Thalassemia has both ineffective erythropoiesis (IE) and hemolysis, IE
is due to accelerated apoptosis of erythroid precursors, and hemolysis is
because of the accelerated peripheral destruction of erythrocytes. The bone
marrow significantly increases the erythroid precursors due to anemia, cause
bone marrow expansion, but frequent transfusion is still needed for
thalassemia major due to above reasons.
The normal red cell life span is 120 days and that the duration of a
reticulocyte in the p... 阅读全帖 |
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l*****y
发帖数: 5737 | 3 ☆─────────────────────────────────────☆
jck (浮萍妈妈) 于 (Wed Feb 20 13:59:02 2008) 提到:
我父亲在被诊断为Myelodysplastic syndrome。现在正在用Amifostine，也考虑用
vidaza。看了网上文章，Amifostine好象还实验阶段。我们对Amifostine的疗效怀疑。
我父身体很弱，反应很大。我想把他的骨髓细胞检验报告翻译到英文好在美找专家问问
。可是我用了好几个小时才翻了几个字，还不确定。请各位能者帮着翻一下，哪怕一个
词也好。谢谢了！
原始粒细胞 15 0-1.0
早幼粒细胞 2 0-2.5
中性中幼粒细胞 18.5 3.2-13.5
中性晚幼粒细胞 17 5.0-20.0
中性杆状核粒细胞 21 8.5-25.0
中性分叶核粒细胞 5 6.1-20.0
嗜酸性中幼幼粒细胞 0-1.1
嗜酸性晚幼粒细胞... 阅读全帖 |
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c**i
发帖数: 6973 | 4 I simply can not understand why Chinese parents from PRC whine.
Somehow I grt the feelings that Chinese parents often regret that they did
not abort their babies who have minor problems.
Can't they just say, "He Ain't Heavy, He's My Brother"?
http://en.wikipedia.org/wiki/He_Ain't_Heavy,_He's_My_
Can't they say I will accept my child no matter what, whether he or she is
gay or something else?
(1) 蒙古斑 Mongolian spot is very common among colored people including
Chinese. Perhaps you yourself, your ... 阅读全帖 |
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s****z
发帖数: 50 | 7 想请教用semi-solid culture, methylcellulose or collagen培养megakaryocyte做
colony formation assay是，怎么区别megakaryocyte和其他细胞，比如erythroid的
colonies啊？ 需要染色吗？
谢谢指教！！！ |
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a******u
发帖数: 211 | 8 Aaron Yun Chen1, Elizabeth Yan Zhang1, Wuxiang Guan1, Fang Cheng1, Steve
Kleiboeker2, Thomas M. Yankee1, and Jianming Qiu1
The small 11kDa nonstructural protein of human parvovirus B19 plays a key
role in inducing apoptosis during B19 virus infection of primary erythroid
progenitor cells. Blood, 2010, 115(5): 1070
please send the paper to e*********[email protected] |
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h******y
发帖数: 1374 | 9 Scientists turn human skin into blood
Scientists at McMaster University have discovered how to make human blood
from adult human skin.
The discovery could mean that people needing blood for surgery, cancer
treatment or treatment of blood conditions like anemia will be able to have
blood created from a patch of their own skin to provide transfusions.
Clinical trials could begin as soon as 2012.
The researchers have also shown that the conversion is direct. Making blood
from skin does not require ... 阅读全帖 |
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V***b
发帖数: 3419 | 10 完了，心彻底凉了，你这个Nrf1是nuclear respiratory factor 1，我说的Nrf1是
Nuclear factor erythroid 2-related factor 1，都是转录因子，但不是一个东西。
Nrf2应该是一样的。最后一棵稻草。 |
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V***b
发帖数: 3419 | 11 Nuclear factor erythroid 2-related factor 1。不是啥流行蛋白，找个抗体都困难。然后mRNA有好几种variant，在蛋白层面有好几种isoform，功能明晰的好像是最大的那个，头疼。
4度存储还好了，抗体结构稳定，不爱坏。 |
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z*t
发帖数: 863 | 12 不仅要做cmp/mep/gmp lt-hsc/st-hsc/mpp staining,还要有完整的lineage analysis:
erythroid: ter119/cd71, myeloid: Gr1, mac1， B cell lineage: CD19, B220 T
cell lineage: CD4,CD8。这些外周血、骨髓，spleen都做一下.对于block of
differentiation,主要还是FACS和giemsa-wright stain来确定。
还有为了排除microenvironment的影响，还要把KO mice的bm移植给wt mice。至于
osteoclast,最近认为lymphnoid progenitor会呆在osteoblast旁边，并受cxcl12调控
。去年的ASH也有人在osteoblast overexpress beta-catenin可以导致leukemia的
same |
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g*********5
发帖数: 2533 | 13 DMSO Promotes the erythroid differentiation of MEL cells |
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f******g
发帖数: 1003 | 14 Reprogramming human endothelial cells to haematopoietic cells requires
vascular induction
Nature
Generating engraftable human haematopoietic cells from autologous tissues is
a potential route to new therapies for blood diseases. However, directed
differentiation of pluripotent stem cells yields haematopoietic cells that
engraft poorly. Here, we have devised a method to phenocopy the vascular-
niche microenvironment of haemogenic cells, thereby enabling reprogramming
of human endothelial cells in... 阅读全帖 |
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z*******6
发帖数: 353 | 15 有2个问题不明白。
In beta thalassemia major, absence of beta-globin chain causes
1,intra-medullary destruction which causes ineffective erythropoiesis.
2, hemolysis/ reticulocytes increase
3, marrow expansion.
我的问题是
1.If the erythropoiesis is ineffective, why we can see increased
reticulocytes ?
2, If the erythropoiesis is ineffective, why we can see marrow expansion which is caused by erythroid hyperplasia ?
麻烦能解释一下？谢谢 |
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A**A
发帖数: 224 | 16 Ineffective erythropoiesis
1. The reticulocyte count is elevated, but it is lower than expected for the
severity of anemia.
2. Severe anemia is uncompensated. It leads to massive erythroid hyperplasia
. |
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