s**********t
发帖数: 680 | 1 Crit Rev Eukaryot Gene Expr. 2000;10(2):179-212.
Title: Chromosome territories, interchromatin domain compartment, and
nuclear matrix: an integrated view of the functional nuclear architecture.
Cremer T, Kreth G, Koester H, Fink RH, Heintzmann R, Cremer M, Solovei I,
Zink D, Cremer C.
Journal link:http://www.begellhouse.com/journals/6dbf508d3b17c437,0e3100b11d4c829b,09458a4d2245ffe0.html
Pubmed link: http://www.ncbi.nlm.nih.gov/pubmed/11186332
Please send it to: b************[email protected]
Thank ... 阅读全帖 |
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s********l
发帖数: 1195 | 2 Keesee K, Briggman JB, Thill G, Wu YJ (1996) Utilization of
nuclear matrix proteins for cancer diagnosis. CRC Crit Rev
Eukaryotic Gene Expr 6:189-214
Thanks! |
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V*******s
发帖数: 19 | 3 Does anyone know how to analyze eukaryotic gene promoter sequence?
Thanks a lot. |
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l*******1
发帖数: 16217 | 4 看你们这二道贩子文章 不如看底下英文UC伯克利是怎么耍臭不要脸的
专利局都说了张峰的专利在真核生物中有效 是一个separate case
UC 伯克利还在那意淫要所有细胞类型中的专利 你让美国专利局自己抽自己的脸吗
Appeals board clears way for UC Berkeley to receive patent on CRISPR-Cas9
gene editing
By Robert Sanders, Media relations | FEBRUARY 15, 2017
Click to share on Twitter (Opens in new window)181Click to share on Facebook
(Opens in new window)181Click to share on Google+ (Opens in new window)
Click to share on Reddit (Opens in new window)Click to email this to a
friend (Opens in new window)... 阅读全帖 |
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w********2
发帖数: 632 | 5 HGT involving eukaryotes
The best recognized examples of HGT in eukaryotes in- clude the wholesale
absorption of bacterial genomes that once existed as endosymbionts or
parasites and now ex- ist as remnant genomes, such as mitochondria and plas-
tids (Gray, 1993). This process of ‘you are what you eat’ (Doolittle,
1998) has continued throughout evolu- tion and includes gene acquisitions
from both prokary- otic (Hotopp et al., 2007) and eukaryotic endosymbionts (
Li et al., 2006; Nosenko and Bhat... 阅读全帖 |
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w********2
发帖数: 632 | 6 Cellular competency (natural transformation) refers to the active uptake of
free (extracellular) DNA across the cell wall and cell membrane(s) using
cellular machinery designed to facilitate the process (Dubnau, 1999; Lorenz
and Wackernagel, 1994). It is a highly regulated physio- logical state that
is often sensitive to environmental cues (Molin and Tolker-Nielsen, 2003).
When the DNA is her- itably incorporated into the recipient’s genome, the
pro- cess is known as transformation.
Bacterial co... 阅读全帖 |
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P*****e
发帖数: 25 | 7 今天有空去查了一下CRISPR专利的历史，UCB的专利已经有claim通过了审查（allow）
，只是还没有issue（时间问题）。我在后面列出来了其中一个通过了的claim，同时把
张锋issue了的专利里面的一个claim也列了出来。大家看能避开不？张锋后来的Cpf1看
着能避开UCB的Cas9。
有意思的是发现最早的一篇CRISPR相关专利申请其实是Luciano A. Marraffini（张锋
合作者）在Northwestern University时申请的，priority date Sep 23, 2008，早了
好几年。看看claim，只有interference，还没进化到切割，可惜一个claim也没批，居
然华丽丽的被一堆112给毙了，也就是审查员觉得那专利写的不清不楚，业内普通人士
看着不可能做出claim的结果，连101，102，103都没用上就挂了。西北也是早早的
abandon了，当年连PCT都没申请，可见并不重视。张锋专利里也没有Marraffini，虽然
science文章有他，我猜可能也是他只做真核interference方面的工作吧，张跟他合作
并进... 阅读全帖 |
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f**d
发帖数: 768 | 8 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
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w********2
发帖数: 632 | 9 HGT involving prokaryotes
Many studies support the significant role of HGT in the evolution of
prokaryotes (Gogarten et al., 2002; Jain et al., 2003; Kunin and Ouzounis,
2003). The principle sources of novel genes are provided by other prokary-
otes, viruses and MGEs (Fig. 1). In particular, plasmids and composite
mobile elements contribute significantly to HGT between prokaryotes. In some
cases, composite elements and megaplasmids are composed of more than 100
genes (Bentley et al., 2002; Hacke... 阅读全帖 |
|
w********2
发帖数: 632 | 10 More surprisingly is the apparent rarity of eukary- otic genes in
prokaryotic genomes (Andersson, 2005; Guljamow et al., 2007; Pilhofer et al.
, 2007; Rogers et al., 2007). The abundant opportunities for prokaryotes to
en- counter eukaryotic genetic material suggest that signifi- cant
functional (e.g. the presence of introns in eukaryotic genes, inefficient
gene transfer mechanisms) and selec- tive barriers have restricted the long-
term acquisition of eukaryotic genes by bacteria. |
|
m*********n
发帖数: 28 | 11 没有找到好的模板，来问问大家在PL里面怎么举例证明自己的文章被引用。
比方说，有一个还不错的文章，引用了我的两篇文章十几次，但是大部分都是都是同时
引用了好几篇文章。比方说下面这个例子：
Eukaryotic Vps4 has a beta-domain inserted into the small ATPase domain(
19,20,24) that recruits the VPS4 activator Vta1p/LIP implicated in assembly
and ATPase activity (21,27，28，29，30). Crenarchaeal Vps4 lacks the beta-
domain (29) and the regulatory factors are absent in Crenarcheota(31)。
我的文章是29， 在这两句话里面一共引用了两次，我该怎么写呢？ 这样写可以么？
Eukaryotic Vps4 has a beta-domain inserted into the small ATPase ... 阅读全帖 |
|
发帖数: 1 | 12 小黄人不吉利啊， 不过细看应该是赢了。
The dispute centred on CRISPR’s usability in two different kinds of
biological lifeforms: prokaryotes and eukaryotes. UC had devised a way to
edit the genetic makeup of prokaryotes using CRISPR in 2014. Broad followed
a year later with a method to use CRISPR in eukaryotes. UC had subsequently
contested the patentability of Broad’s method saying that it was a
derivative of UC’s method and couldn’t be patented separately. |
|
发帖数: 1 | 13 Argonaute (Ago) proteins are complexed with small DNA or RNA guides and
cleave nucleic 24
acid targets whose sequences are complementary with the guides1. Agos in
eukaryotes bind 25
to small interfering RNAs (siRNAs) or microRNAs (miRNAs) to suppress gene
expression by 26
degrading mRNAs in a targeted manner, a regulatory process known as RNA
interference 27
(RNAi). Agos in eubacteria and archaea are complexed with DNA or RNA guides
and function 28
as defense systems against foreign mobile genet... 阅读全帖 |
|
L**y
发帖数: 40 | 14 有意者请寄简历到l***[email protected]
Title: Research Associate- Cell Culture
Local candidates only. PhD’s NOT being considered.
About us
Biotechnology Company locates in North Dartmouth, 40 miles south of Boston,
just minutes from University of Massachusetts-Dartmouth.
Job Description
We have an immediate opening for a part-time and possible convert to full-
time Research Associate in the Cell Biology field.
The primary role of this position includes cell culture, prepare and stock
media, maintenance of mult... 阅读全帖 |
|
L**y
发帖数: 40 | 15 【 以下文字转载自 JobHunting 讨论区 】
发信人: Lexy (Lei), 信区: JobHunting
标 题: 麻省南部小生物公司需part time一名，local only！
发信站: BBS 未名空间站 (Thu May 19 15:30:50 2011, 美东)
有意者请寄简历到l***[email protected]
Title: Research Associate- Cell Culture
Local candidates only. PhD’s NOT being considered.
About us
Biotechnology Company locates in North Dartmouth, 40 miles south of Boston,
just minutes from University of Massachusetts-Dartmouth.
Job Description
We have an immediate opening for a part-time and possible convert to full-
time Re... 阅读全帖 |
|
c*******e
发帖数: 4642 | 16 我我我，实在忍不住了。。。。。。。。
细菌是单细胞木有细胞核的,called prokaryote，即所谓原核。有细胞核的，比如酵母
，called eukaryote,即所谓真核
一般定义上，起码俺学的microbiology是说，parasite是所有eukaryotic organisms
细菌，病毒都排除在外的 |
|
m**i
发帖数: 47 | 17 Scientist I, Biology
Location：Beijing
Job Responsibility:
Responsible for cell culture media and process development works in labs
using standard cell culture instruments and technologies. Performs duties
independently with only general direction given on new projects and methods.
Accuracy and dedication are required in performing all functions of this
position. Apply laboratory techniques and skills to complete experiments
designed to address a variety of specific problems. Make detailed
observ... 阅读全帖 |
|
s**l
发帖数: 11983 | 18 http://www.nature.com/nature/journal/v470/n7334/full/nature0981
An early Ediacaran assemblage of macroscopic and morphologically
differentiated eukaryotes
地球上最古老的「高等生命」到底是什么样子?人类已知的高等生命从什么时期开始演
化?英国的《自然》(Nature)杂誌在17日公佈了这一重大发现。
地球上的生命从无到有、从简单到复杂，其中的进化过程相当漫长。古生物学家认
为，在现今生物圈中，包括我们人类在内的所有肉眼可见的生命，几乎都是多细胞宏体
生物，也就是常说的「高等生命」，而多细胞宏体生物的出现是地球生命进化史上极为
重要的事件。
但是学术界一直认为地球没有早于5.8亿年前的「高等生命」，不过中国科学家在
安徽休甯县蓝田镇找到的「蓝田植物群」把高等生命起源向前推进了近4000万年。
「蓝田植物群」就在知名的景点黄山附近，出现在6亿多年前，展现了早期动物大规模
出现前夕的生命景观，是地球早期生命从简单向复杂进化过程中的重要环节。... 阅读全帖 |
|
p*******r
发帖数: 59 | 19 Career Opportunities at JCVI Infectious Disease (8 Positions)
The PIs did not ask me to post here, I did this just in case someone is
looking for similar positions but did not see these, and I would be happy to
see some chinese fellows to come. All the PIs are very nice.
All listed at
https://careers.jcvi.org/careers/Careers.aspx
http://www.jcvi.org/
No. 1 & No. 2 Post-Doctoral Fellows
Job Responsibilities
JCVI is looking for two Post-Doctoral Fellows to join the Infectious Disease
Group in our ... 阅读全帖 |
|
e*****r
发帖数: 1229 | 20 E。 Coli细胞表面上有 TAR 和TSR等receptor，这些receptor
是细胞接受外界化学信号的接受器。 那么比较大的细胞，例如eukaryotic细胞， 表面
上的receptor有那些哪？我查阅了文献， 大家都讨论E. Coli， 没讨论eukaryotic细
胞的。多谢了 |
|
s******a
发帖数: 472 | 21 http://www.cell.com/molecular-cell/fulltext/S1097-2765(11)00331
Fifty Years after Jacob and Monod: What Are the Unanswered Questions in Molecular Biology?
=====================================================================
Tomorrow's Molecular Biology
Marc W. Kirschner
Harvard University
Today, the term molecular biology has a rather prosaic sound. After all,
there is little in contemporary biology that isn't molecular. It's hard to
remember that 50 years ago, molecular biology was distinctly ... 阅读全帖 |
|
r*****t
发帖数: 4793 | 22 Nature. 2012 Mar 14;483(7390):434-8. doi: 10.1038/nature10895.
Intrinsic coupling of lagging-strand synthesis to chromatin assembly.
Smith DJ, Whitehouse I.
Source
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York
Avenue, New York, New York 10065, USA.
Abstract
Fifty per cent of the genome is discontinuously replicated on the lagging
strand as Okazaki fragments. Eukaryotic Okazaki fragments remain poorly
characterized and, because nucleosomes are rapidly deposited on n... 阅读全帖 |
|
l**********1
发帖数: 5204 | 23 Bingo
人的血红细胞的生物钟韵律调控都可以不用DNA 的 transcription-translation feedback loops
各个血红细胞应该不会只有一套proteomics 不然生物钟韵律调控如何实现?
Nature. 2011 Jan 27;469(7331):498-
Circadian clocks in human red blood cells.
//www.ncbi.nlm.nih.gov/pubmed/21270888
full text link:
//www.dormivigilia.com/wp-content/uploads/2011/02/nature09702.pdf
Abstract
Circadian (～24 hour) clocks are fundamentally important for coordinated physiology in organisms as
diverse
as cyanobacteria and humans. All current models of the molecular circadi... 阅读全帖 |
|
T*****e
发帖数: 247 | 24 http://www.nature.com/nature/journal/vaop/ncurrent/full/nature1
标题 DNA-guided DNA interference by a prokaryotic Argonaute
摘要
RNA interference is widely distributed in eukaryotes and has a variety of
functions, including antiviral defence and gene regulation1, 2. All RNA
interference pathways use small single-stranded RNA (ssRNA) molecules that
guide proteins of the Argonaute (Ago) family to complementary ssRNA targets:
RNA-guided RNA interference1, 2. The role of prokaryotic Ago variants has
r... 阅读全帖 |
|
发帖数: 1 | 25 Broad 已经辟谣了
https://www.broadinstitute.org/what-broad/areas-focus/project-spotlight/
journalists-statement-and-background-crispr-patent-interfer
Update: August 17, 2016
In its Opposition Document 2, posted August 16, the Regents of the
University of California again claim Broad relied on Jinek 2012 to engineer
a eukaryotic CRISPR-Cas9 gene editing system.
This is false, and has been demonstrated to be false numerous times in the
public record.
In an attempt to strengthen its case, in Opposition ... 阅读全帖 |
|
发帖数: 1 | 26 Terminators in eukaryotes[edit]
In eukaryotic transcription of mRNAs, terminator signals are recognized by
protein factors that are associated with the RNA polymerase II and which
trigger the termination process. Once the poly-A signals are transcribed
into the mRNA, the proteins cleavage and polyadenylation specificity factor
(CPSF) and cleavage stimulation factor (CstF) transfer from the carboxyl
terminal domain of RNA polymerase II to the poly-A signal. These two factors
then recruit other pr... 阅读全帖 |
|
s********n
发帖数: 26222 | 27 方舟子在此文中说“虫子不吃的， 人能吃吗”是中国人的质疑， 而美国人是不会问这
样的问题的， 这显然是骗人的。见下文和其中引用的文献， 其中不少是美国人的科研
。
"Comments on the human health impact of Bacillus thuringiensis toxin gene
product in genetically modified crops"
EPA Review Docket Number OPP-00678B
Professor Joe Cummins
Professor Emeritus of Genetics
University of Western Ontario
I have studied the Environment Protection Agency (EPA) document "Bt Plant-
Pesticides Biopesticides Registration Action Document B. HUMAN HEALTH
ASSESSMENT" and comment in the following ... 阅读全帖 |
|
g*********o
发帖数: 4653 | 28 http://en.wikipedia.org/wiki/Transformation_(genetics)
In molecular biology transformation is the genetic alteration of a cell
resulting from the direct uptake,
incorporation and expression of exogenous genetic material (exogenous DNA)
from its surroundings and taken up through the cell membrane(s).
Transformation occurs naturally in some species of bacteria,
but it can also be effected by artificial means in other cells.
Bacteria that are capable of being transformed, whether naturally or
artif... 阅读全帖 |
|
M******8
发帖数: 10589 | 29 http://my.cnd.org/modules/wfsection/article.php?articleid=35359
---- 为什么要避免基因改造过的食物（Genetically Modified （GM or GMO） Foods
）----
1。GMO 食物的潜在危害没有搞清楚：基因从结构和功能上来看，是地球生命（包括人
）活动中最稳固的一类；尽管，基因随时发生变异和损伤，但基因的修复和调节功能也
是非常强大的[1, 2]。从宏观上看，一个遗传性状的明显改变往往要经过很多代生物的
演变才能发生 (对人来说，这就意味着数十到数百年的时间)，更何况，现代分子生物
学的知识大部分是从低等生物，甚至原核生物（如大肠杆菌）实验中得来的[3]。请各
位想想，经过美国FDA严格把关，还经过了临床人体试验的各种药物，不少在几年至十
几年后，又发现新的危害，甚至致命，医药公司不得不赔钱，回收药物，或者，贴上“
在医生指导下慎用”的标签。用基因改造技术制造食品，没有任何长期检验，效果又要
数十年甚至数百年才能显现，基因改造技术的应用也只有一，二十年的时间，就投放市
场，这市场经济利润至... 阅读全帖 |
|
a*******s
发帖数: 117 | 30 大部分的变异都是近中性的。要移除近中性突变需要非常长的时间，也就是大部分被水
平转入的基因（在一个很大的时间尺度内）都处在“被移除”或者“被固定”过程中，
这个过程是极其缓慢的，这些基因一直没有被移除而处于实际存在状态。
比如金小蜂（Nasonia)体内被转入了细菌（Walbachia)的近完整基因组。
Hotopp, Julie C. Dunning, et al. "Widespread lateral gene transfer from
intracellular bacteria to multicellular eukaryotes." Science 317.5845 (2007)
即使这个被转入的“寄生基因组”会降低金小蜂的适应度（fitness），要移除这些基
因是需要大的时间尺度的。以致人类能够观察到金小蜂的体内存在有异常的基因（若被
快速移除则人类无法观察到）。 |
|
w*p
发帖数: 16484 | 31 蛋白结构得诺奖：
2003: Peter Agre and Roderick MacKinnon (Chemistry), Agre "for the
discovery of water channels" and MacKinnon "for structural and mechanistic
studies of ion channels". Structures by MacKinnon and his group began in
1998 with the transmembrane potassium channel 1bl8, and continued with many
others. After this prize, Agre was involved in the structures reported in
2005, 2f2b and 2evu.
2006: Roger Kornberg (Chemistry) "for his studies of the molecular basis
of eukaryotic transcript... 阅读全帖 |
|
f**x
发帖数: 4325 | 32 世界上哪个单项科研成果不是“就那么回事儿”？
下面这些又牛逼了多少？怎么就不是“就那么回事儿”了？
The Nobel Prize in Chemistry 2014
Eric Betzig, Stefan W. Hell and William E. Moerner
"for the development of super-resolved fluorescence microscopy"
The Nobel Prize in Chemistry 2013
Martin Karplus, Michael Levitt and Arieh Warshel
"for the development of multiscale models for complex chemical systems"
The Nobel Prize in Chemistry 2012
Robert J. Lefkowitz and Brian K. Kobilka
"for studies of G-protein-coupled receptors"
The Nobel Prize in Chemist... 阅读全帖 |
|
l******n
发帖数: 11737 | 33 http://www2.mrc-lmb.cam.ac.uk/ccsb/?page_id=20
注意第二段第一句。
Jason Chin is currently a Programme Leader at the Medical Research Council
Laboratory of Molecular Biology (MRC-LMB), where he is also Head of the
Centre for Chemical & Synthetic Biology (CCSB). He is Professor of
Chemistry & Chemical Biology at the University of Cambridge, and holds a
joint appointment at the University of Cambridge Department of Chemistry.
He is also a fellow in Natural Sciences at Trinity College, Cambridge.
Jason is ... 阅读全帖 |
|
r*s
发帖数: 2555 | 34 【 以下文字转载自 Joke 讨论区 】
发信人: rbs (jay), 信区: Joke
标 题: 诺委会太不像话了
发信站: BBS 未名空间站 (Wed Oct 4 10:23:52 2017, 美东)
尼玛87篇参考文献就不能加一篇施教授或能教授的CNS？
References
1. Ruska, E., Nobel Lectures, Physics 1981-1990, Tore Frängsmyr and
Gösta Ekspong, Eds. (1993) World Scientific Publishing, Singapore
2. Marton, L. (1934) Electron microscopy of biological objects. Nature
133, 911-911
3. Althoff, T., Mills, D. J., Popot, J. L., and Kühlbrandt, W. (2011)
Arrangement of electron transport chain compone... 阅读全帖 |
|
x****6
发帖数: 4339 | 35 大分子照相相关的炸药奖加上这次的，已经发了14个了（见下表）。
之前主要是基于X衍射或者核磁共振的技术开发和应用，拍一些单分子的裸照。82，91
，02年颁给X衍射和核磁共振的技术开发，剩下11个的给了应用。
而冷冻电镜，这次算是第一个（可能是第二个），直接给了技术。用它可以看更加复杂
、分子量更大的大分子复合体。这些大个头的结构当然更有复杂也更有意思。
这个技术开启了一个新时代，细胞里重要的复合体实在太多。接下来可能拿奖的那就是
：真核细胞的细胞核通道复合体；一一公搞的剪接体（这个结构无论在生命进化和疾病
相关都非常的重要）；G蛋白偶联受体（跟N多疾病有关）等等。
彦宁的工作好像确实没有什么希望，葡萄糖转运蛋白跟上面这些不是一个量级的。
------------------------
2017: Chemistry cryoEM development
2009: Chemistry Venkatraman Ramakrishnan, Thomas A. Steitz, Ada E.
Yonath Studies of the structure and funct... 阅读全帖 |
|
h*****x
发帖数: 147 | 36 2006: Chemistry Roger D. Kornberg Studies of the molecular basis of
eukaryotic transcription |
|
h*****x
发帖数: 147 | 37 DNA和RNA合成的机理1959年就发奖了，看来中心法则相关的一个都不会落
: 2006: Chemistry Roger D. Kornberg Studies of the molecular
basis of
: eukaryotic transcription
|
|
w********2
发帖数: 632 | 38 对啊，e coli的祖先很简单，怎么能够进化成人呢？
染色体是怎么进化出来的？为啥要染色体啊？好像只有eukaryotic cells 才有。
线粒体来源于细胞好像是一种hypothesis，不是科学事实。 |
|
w********2
发帖数: 632 | 39 In the short term, HGT can increase genetic diversity and promote the spread
of novel adaptations between organisms (Marri et al., 2007; Thomason and
Read, 2006). HGT has been a major contributory factor to the rapid spread of
antibiotic re- sistance amongst pathogenic bacteria in the last 50 years (
Mazel and Davies, 1999), and the emergence of in- creased virulence in
bacteria, eukaryotes and viruses (Derbise et al., 2007; Friesen et al., 2006
; Mild et al., 2007). In the long term it has been... 阅读全帖 |
|
w********2
发帖数: 632 | 40 Phylogenetic analysis
Phylogenetic analysis of gene or protein sequences for the presence of an
incongruent phylogenetic signal is considered to represent the most rigorous
method for de- tecting past HGT events (Koonin et al., 2001; Syvanen, 1994)
. Smith et al. (1992) established the basic require- ments for application
of the phylogenetic congruency test to identify and support cases of HGT.
The test com- pares the phylogenetic tree constructed from a specific
protein or gene sequence with th... 阅读全帖 |
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w********2
发帖数: 632 | 41 Inconsistent evolutionary scenarios
Deriving a parsimonious evolutionary scenario is based on the premise that
over time, genetic change is relent- less, such that distant relatives are
expected to have fewer features in common than close relatives (Koonin et al
., 2001). Therefore, the presence of some distinguishing ge- netic signal in
a distant relative but absent from more closely related organisms
contradicts this expectation. The most common causes of these anomalies are
loss of the geneti... 阅读全帖 |
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w********2
发帖数: 632 | 42 Mobile genetic elements
MGEs are non-essential (accessory) genomic elements composed of genes and
structural features that facil- itate their spread both within and between
organisms (Doolittle and Sapienza, 1980; Orgel and Crick, 1980). They are
found in the genomes of all prokaryotes and eukaryotes. MGEs share many
features and often share a common ancestry with viruses. The main difference
is that viruses are usually capable of extracellular persis- tence.
MGEs, including defective forms, are... 阅读全帖 |
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w********2
发帖数: 632 | 43 Translocation of genetic material
The major mechanisms of HGT described in the literature include conjugation,
cellular competency (natural trans- formation) and virus-mediated
transduction (Chen et al., 2005; Dreiseikelmann, 1994; Dubnau, 1999).
However, these mechanisms only refer to the first step of any suc- cessful
gene transfer event, namely the passage of ge- netic material across
cellular barriers. Independent mech- anisms are involved in integrating the
new genes into the genome of the ... 阅读全帖 |
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发帖数: 1 | 44 Abstract
We show here that, unlike most other prokaryotic Argonaute (Ago) proteins,
which are
DNA-guided endonucleases, the Natronobacterium gregoryi-derived Ago (NgAgo)
can
function as a DNA-guided endoribonuclease, cleaving RNA, rather than DNA,
in a
targeted manner. The NgAgo protein, in complex with 5’-hydroxylated or 5’-
phosphrylated oligodeoxyribonucleotides (ODNs) of variable lengths, split
RNA targets
into two or more fragments in vitro, suggesting its physiological role in
bacteria and... 阅读全帖 |
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m*********a
发帖数: 3299 | 45 Jews .2% of world population, 20% of all Nobel prices, 40% of USA nobel
price, won more than 160 nobels.
All Asian are junk compare to Jewish people in your standard.
Literature[edit source | edit]
Year Laureate Country Rationale
1927 Bergson-Nobel-photo.jpg Henri Bergson[9][10] France "in
recognition of his rich and vitalizing ideas and the brilliant skill with
which they have been presented"[11]
1958 Boris Pasternak cropped.jpg Boris Pasternak[9][10] Sov... 阅读全帖 |
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A********e
发帖数: 354 | 46 【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖 |
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b**********t
发帖数: 57 | 47 I am in the field of biophysics, more specifically biomechanics. I have
extensive experience with AFM, optical trap, magnetic tweezers; eukaryotic
cell and bacterial biomechanics.
Now I am in the process of green card application. I need to accumulate my
review experience.
I have reviewed papers from:
1. European Biophysical Journal,
2. Biosensors,
3. Journal of Mechanics in Medicine and Biology,
4. Journal of Nanomaterials.
Journals that I have publications：
1. biophysical journal,
2. physical... 阅读全帖 |
|
b**********t
发帖数: 57 | 48 I am in the field of biophysics, more specifically biomechanics. I have
extensive experience with AFM, optical trap, magnetic tweezers; eukaryotic
cell and bacterial biomechanics.
Now I am in the process of green card application. I need to accumulate my
review experience.
I have reviewed papers from:
1. European Biophysical Journal,
2. Biosensors,
3. Journal of Mechanics in Medicine and Biology,
4. Journal of Nanomaterials.
5. Plos One
Journals that I have publications：
1. biophysical journal,... 阅读全帖 |
|
b**********t
发帖数: 57 | 49 I am in the field of biophysics, more specifically biomechanics. I have
extensive experience with AFM, optical trap, magnetic tweezers; eukaryotic
cell and bacterial biomechanics.
Now I am in the process of green card application. I need to accumulate my
review experience.
I have reviewed papers from:
1. European Biophysical Journal,
2. Biosensors,
3. Journal of Mechanics in Medicine and Biology,
4. Journal of Nanomaterials.
Journals that I have publications：
1. biophysical journal,
2. physical... 阅读全帖 |
|
Y****N
发帖数: 8694 | 50 An astonishingly small number of elite universities produce an overwhelming
number of America’s professors.
By Joel Warner and Aaron Clauset
The United States prides itself on offering broad access to higher education
, and thanks to merit-based admissions, ample financial aid, and emphasis on
diverse student bodies, our country can claim some success in realizing
this ideal.
The situation for aspiring professors is far grimmer. Aaron Clauset, a co-
author of this article, is the lead author of ... 阅读全帖 |
|
