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全部话题 - 话题: exportin
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m******5
发帖数: 1383
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Gontan, C., T. Guttler, et al. (2009). "Exportin 4 mediates a novel nuclear
import pathway for Sox family transcription factors." J Cell Biol 185(1): 27
-34.
SRY and other Sox-type transcription factors are important developmental
regulators with various implications in human disease. In this study, we
identified Exp4 (exportin 4) as an interaction partner of Sox2 in mouse
embryonic stem cells and neural progenitors. We show that, besides its
established function in nuclear export, Exp4 acts
m******5
发帖数: 1383
2
来自主题: Biology版 - 投什么杂志?
楼主是专门做转运的?
可以考虑做 Invitro-nuclear import assay
就是用纯化的转运体系在digitonin permeablized 的Hela 细胞核上做。
因为整个体系都是体外重建的,理论上不会出现非直接影响的情况
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261132/
不过好奇地问一下,这个化合物阻断核转运你们是propose和哪个转运蛋白结合?
importin beta? alpha? or exportin?
如果真的只特异某个subtype的importin的活性的话还是很有意思的,不过鉴于楼主能
在oocyte里做出来,可能性不大。
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