r****r
发帖数: 379 | 1 挺喜欢骆的东西, his new cell paper
－－－－－－－－－
* Highlights
* The Q system offers repressible binary gene expression in flies and
mammalian cells
* Q-MARCM enables mosaic cell labeling and genetic manipulation
independently of GAL4
* Coupled MARCM independently labels and manipulates both progeny of a
cell division
* Intersecting Q and GAL4 systems refines transgene expression for
functional analyses
We describe a new repressible binary expression system based on the
regulatory genes f |
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x*********a
发帖数: 906 | 2 E. coli host genotype has to be lamda+?
lamda- strains can not express T7 promoter-direced gene from plasmid?
Any other prophage on E. coli K12 has T7 RNA polymerase?
How about SP6 promoter? what kind of genotype can not express SP6 promoter ?
Can this genotype E. coli K12 ∆(araD-araB)567, ∆lacZ4787(::rrnB-
3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514 express SP6 or T7
promoter-driven gene?
Thanks a lot. |
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h***e
发帖数: 232 | 3 Just wondering if there is a way to quantify a specific gene expression in
mixed population of cells.
For example:
Each sample has x number of cells from normal tissue mixed with y number of
cancer cells. x and y are unknown.
How to compare some specific gene expression profiles (those genes only
expressed in cancer cells) among different
samples.
Any reference? |
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h***e
发帖数: 232 | 4 Many people normalize the expression to the housekeeping genes expression;
but what if I don't know how many normal/cancer cells, considering both
normal/cancer cell contribute to the housekeeping gene expression?
heterogeneous? |
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h******1
发帖数: 94 | 5 In the manual,
http://www.broadinstitute.org/gsea/doc/GSEAUserGuide.pdf
On the page 7, right above "Phenotype Labels", it says
"The GSEA algorithm does not filter the expression dataset and does not
benefit from your filtering of the expression dataset. During the analysis,
genes that are poorly expressed or that have low variance across the dataset
populate the middle of the ranked gene list and the use of a weighted
statistic ensures that they do not contribute to a positive enrichmen |
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S******y
发帖数: 1123 | 6 I am new to Gene Expression problem. But I am very interested in the
field.
But is Gene Expression problem (most of time) equivalent to variable
selection problem?
Have the following methods been widely used in Gene Expression problem?
- SSVS (Stochastic search variable selection)
- LASSO
- Stepwise
Thanks! |
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w*******y
发帖数: 60932 | 7 Express has 20% off their clearance pricing online right now. There are some
great deals, $24 for jeans that are $80 regularly priced.
Women's Sale:
http://www.express.com/sale-43.cat?ICID=2163
Men's Sale:
http://www.express.com/sale-80.cat?ICID=2164
The additional 20% off is taken off of the clearance price and is applied in
the shopping bag.
Shipping for my $150 order (about 10 items) was $8.
I searched and didn't see this deal linked yet, hope this isn't a repost.
|
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w*******y
发帖数: 60932 | 8 Link:
http://www.express.com
Valid until 9/6/2010
Express is having a rare event where they are having a promotion of both 40%
off of Graphic Tees / Polos and $15 off every $60 spent using Online Promo
Code: 2974
They normally only run one of these promotions at a time.
To print out coupon and use in store:
Link:
http://www.express.com
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w*******y
发帖数: 60932 | 10 Today only using coupon code U3D8B4J at www.bn.com:
http://www.bn.com
Lego Harry Potter Hogwarts Express is 61.15 . You MIGHT have to be a member
for the initial 10% off but anyone can use the coupon code listed above .
Member total is 61.15 but nonmember will be higher around 68. Free shipping
and if you use a favorite shopping site, an additional percent off as well.
TRU has it for 89 and Amazon has it for around 75. So this is a great deal
for me!!
Link:
http://gifts.barnesandnoble.com/Toys-g... 阅读全帖 |
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w*******y
发帖数: 60932 | 12 To be eligible for this offer, you must register your eligible American
Express Card between November 8, 2010 and December 31, 2010. You may only
register one Card for this offer. Using your registered American Express
Card, you must spend $25 or more at one or more participating small business
merchants between 11/27/2010 and 12/31/2010 to receive the $25 statement
credit.
Eligible merchants include any independently owned small businesses that
accept the American Express Card. Purchases made... 阅读全帖 |
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w*******y
发帖数: 60932 | 16 Crest 2 Hour Express Whitestrips and 3D White Rinse Bundle Pack $29.99.
Price dropped back down on the others also, 2 Hour w/o Rinse $26, Advanced
Seal $26.89 free ship Amazon
Last sale was for the 2 Hour Whitestrips, Amazon has now added the Bundled
pack.
Product Description
Crest2 Hour Whitestripsuse the Advanced Seal, no-slip technology that
allows you to talk, drink water and go about your day while you whiten with
no sticky mess. They're also enamel-safe and use the same effective
whitening... 阅读全帖 |
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w*******y
发帖数: 60932 | 17 To celebrate its new Golden Treasure Shrimp entree coming on March 2, Panda
Express is offering a free Golden Treasure Shrimp entree serving on March 9,
via a coupon on its Facebook page.
Plan your lunch now--the coupon has not yet been posted at Facebook, but
will be coming soon and can be obtained by liking Panda Express at its
Facebook page.
Golden Treasure Shrimp: crispy tempura shrimp and red and green bell
peppers wok-tossed in a zesty citrus sauce.
Link:
http://www.restaurantnews.com/pa... 阅读全帖 |
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w*******y
发帖数: 60932 | 18 Crest 3D White Whitestrips With Advanced Seal Professional Effects Enamel
Safe Dental Whitening Kit, 20-count Carton $22.44, 2 Hour Express
Whitestrips $22.26. Free shipping with Prime (FSSS) from Amazon
Start here and look for $10 off Coupon. Note NY'ers not eligible Sry.
Link:
http://www.amazon.com/gp/feature.html?ie=UTF8&docId=1000490791
Crest 3D White Whitestrips With Advanced Seal Professional Effects Enamel
Safe Dental Whitening Kit, 20-count Carton
Link:
http://www.amazon.com/Crest-Whit... 阅读全帖 |
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w*******y
发帖数: 60932 | 20 Groupon is back at it with their Blockbuster Express 5 coupon codes for $2.
Link:
http://www.groupon.com/deals/blockbuster-express-abilene?
.
Coupon codes are valid through May 1, 2013. Limit 1 per person. May buy more
as gifts. Valid only at any Blockbuster Express kiosks and for online
reservation for pickup at kiosks. May use over multiple visits. Not valid on
Blu-ray or Hot List titles. Groupon value reduces by $3 after 5/1/2013,
except where prohibited by law
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w*******y
发帖数: 60932 | 23 quote
LANsurveyor Express for MicrosoftVisiois a powerful Visio 2007 plug-in
that makes it easy to get comprehensive network maps in just minutes!
Accessible via the Visio toolbar, LANsurveyor Express automatically
discovers your network and produces topology maps in Visio format. Plus,
LANsurveyor Express uses Visio SmartShapesto represent your network
devices and populates each node's custom properties with the node's IP
address, network name, and fully-qualified domain name. |
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w*******y
发帖数: 60932 | 24 HighPoint Rocket 620-OEM PCI-Express 2.0 x1 Low Profile SATA III (6.0Gb/s)
Controller Card.
Newegg 9.99 = Free Shipping
Link:
http://www.newegg.com/Product/Product.aspx?Item=N82E16816115099
9.99+Free Shiping.
Great price on this card. Have been waiting for a good price to get one.
Features
PCI-Express 2.0 x1 (Compatible with PCI-Express 1.0)
Serial ATA III (6.0Gbps) compliant, with speed negotiation Serial ATA II (
3Gbps) and Serial ATA I (1.5Gbps)
600MB/s per SATA port
Industry Standard AHCI ... 阅读全帖 |
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w*******y
发帖数: 60932 | 25 Link:
http://www.mgmvegasyourway.com/#
#" rel="nofollow" target="_blank" onclick="_gaq.push(['_trackEvent', 'thread
', 'click', '3814098 - mgm-vegas-properties-american-express-save-up-to-100-
up-to-15-off-room-rates']);">http://www.mgmvegasyourway. com/#
MGM Resorts is offering exclusive savings at 13 of its Las Vegas resorts for
American ExpressCardmembers. Here's how you can take advantage of the
savings:
1) Choose your destination: Explore and choose from MGM Resorts' 13 amazing
Las Vegas d... 阅读全帖 |
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w*******y
发帖数: 60932 | 27 PC Connection Express has the HP ProLiant N40L Ultra Micro Tower Server
System (658553-001) for $180 with free shipping.
Features:
AMD 1.5GHz Turion Neo X2 N40L / 1 processor(s) installed / 1 processor(s)
supported
2GB installed RAM / Technology is DDR3 SDRAM / 8GB maximum / 2MB installed
cache / 250GB hard drive
No Operating System Installed / Ultra Micro Tower / Supports Gigabit
Ethernet Networking
Link:
http://www.pcconnectionexpress.com/IPA/Shop/Product/Detail.htm? Brands - Ben's Bargain Cen... 阅读全帖 |
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w*******y
发帖数: 60932 | 28 Link:
http://www.express.com/content.jsp?pageName=ticktock-timedsale&
Looks like Express has a "secret" sale going online tonight at 11:00EST.
They have done this a couple times before, just cross your fingers the deals
aren't as crappy as last time (http:// />
Here are a few coupon codes I got in the latest catalog that are better than
those listed on the Express website:
$30 off $90: 1119206472006335
$20 off $60: 1119206472006335
$10 off $30: 1119206472006335
$40 off $120: 1119206472006337
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w*******y
发帖数: 60932 | 29 $1 each way between major cities on greyhound by booking 3 weeks ahead (
promotional and for a limited time, so hurry). They're promoting their "
Express" line which uses the newer buses with leather seats, wifi, more
legroom. The "Express" routes don't stop at small towns so you get to your
destination faster. They've always had a few schedules a day like this but
now they've allocated the new buses to them. For trips closer than 3 weeks
out, it's still significantly cheaper than before. H... 阅读全帖 |
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s*****t
发帖数: 1994 | 30 Express to Mars
Credit: Mars Express, ESA
Explanation: Hurtling toward its destination, the high resolution camera on board ESA's Mars Express spacecraft recorded this tantalizing view of the Red Planet earlier this month on December 3rd. Seen from a distance of 5.5 million kilometers, features
across part of Mars' western hemisphere are bathed in sunlight. The Martian night side is also prominent from the spacecraft's perspective, a view not possible for Earthbound telescopes. Launched on an in |
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l*****s
发帖数: 798 | 31 Dalai Lama expresses displeasure over
imprisonment of Chinese dissident
2009-12-29 19:30:00
The 14th Dalai Lama on Tuesday expressed his
displeasure over the imprisonment of Chinese
dissident Liu Xiaobo for 11 years.
He urged Chinese authorities to release
Xiaobo and other political prisoners.
Tibetan Government-in-exile spokesperson
Thubten Samphel said : "His Holiness said he
was saddened by this imprisonment and
requested the Chinese government to release
him (Liu Xiaobo) and all political
p |
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w*********r
发帖数: 42116 | 32 7-Day Unlimited Pass不能用在EXPRESS上，我怎么知道哪个是EXPRESS。比如从
LaGuardia Airport去Manhattan的M60 SBS。 |
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n*********7
发帖数: 4682 | 33 地铁express没关系，这个express是指bus, X开头的 |
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w*p
发帖数: 16484 | 34 Corrected online 28 November 2016
The editors of Nature Biotechnology are issuing an editorial expression
of concern regarding this article to alert our readers to concerns regarding
the reproducibility of the original results. At this time, we are
publishing the results of three groups http://dx.doi.org/10.1038/nbt.3753 that have tried to reproduce the results in the critical Figure 4 in the original paper by Han and colleagues, which demonstrates editing of endogenous genomic loci in mam... 阅读全帖 |
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L*****d
发帖数: 5093 | 35 North Korean leader Kim Jong Un sent a "personal letter" to South Korean
President Moon Jae-in Wednesday, Blue House spokesman Yoon Do-hyun said
Thursday.
In the letter, Kim conveyed "his message of comfort" to the South Korean
people who are battling the outbreak of the novel coronavirus, Yoon said.
Kim said he remains confident that South Korea will prevail in this fight "
without fail" and wished for good health of the "brethren in the South,"
Yoon added.
The North Korean leader also expresse... 阅读全帖 |
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g********d
发帖数: 4174 | 36 NY Leaders Tweaking Marriage Equality Amendments; Optimism Expressed on
Senate Vote Tonight
The latest report following a meeting of Governor Andrew Cuomo, Senate
Majority Leader Dean Skelos, and Assembly speaker Sheldon Silver from the
Times Union's Jimmy Vielkind:
Ny “We’re closing the few, outstanding, technical issues on rent
regulations,” said Skelos, R-Long Island. He predicted action on everything
“hopefully today but certainly by tomorrow.”
Skelos also hinted that his conference and Cuom... 阅读全帖 |
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l****z
发帖数: 29846 | 37 http://www.washingtonpost.com/wp-dyn/content/article/2010/10/13/AR2010101303634.html
A new analysis of political signs displayed at a tea party rally in
Washington last month reveals that the vast majority of activists expressed
narrow concerns about the government's economic and spending policies and
steered clear of the racially charged anti-Obama messages that have helped
define some media coverage of such events.
Emily Ekins, a graduate student at UCLA, conducted the survey at the 9/12
Taxpa... 阅读全帖 |
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l****z
发帖数: 29846 | 38 http://www.express.co.uk/posts/view/213573/Get-Britain-out-of-Europe
THE Daily Express today becomes the first national newspaper to call for
Britain to leave the European Union.
From this day forth our energies will be directed to furthering the cause of
those who believe Britain is Better Off Out.
The famous and symbolic Crusader who adorns our masthead will become the
figurehead of the struggle to repatriate British sovereignty from a
political
project that has comprehensively failed.
After f... 阅读全帖 |
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l*********o
发帖数: 3091 | 39 有很多议论，你在Holiday Inn Express酒店睡觉。觉得怎样？
是的。一晚。实际上是两个晚上。我认为很不错。很干净，很好，床很舒服。这就是我
所需要的。你知道，我不需要佛罗里达棕榈滩。我要赢。我想使我们的国家强大。我想
献出我的精力和这种多年来使事情办得非常好能力。
There is a lot of buzz that you slept in a Holiday Inn Express. How did that
go?
I did. One night. It was actually 2 nights. I thought it was terrific. It
was clean, It was nice and the bed was good. That is all I need. You know, I
don't need Mar-A-Lago. I want to win. I want to make our country great. I
want to devote my energy what ever this ability over t... 阅读全帖 |
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h****e
发帖数: 71 | 40 最近发现在ZAG.com上有几个program，其中一个是American Express Network Auto
Purchasing Program。从这个program进去可以拿到比较低的价格。版上有人用过吗？
是不是一定需要是American Express的Card member呢？ |
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s********y
发帖数: 1547 | 41 请教：15座的express 和E350那个更好一些
从空间上看差不多了
但是新车好像express贵一些
如果是进二手的，那个能开的更久一些，，我是说上25K
对于美国大车没概念 |
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l*****6
发帖数: 752 | 42 请问谁用过American Express 行用卡的租车保险 （premium car rental protection
）claim 的。
租车出事故，以为是American Express是负责，不知道为什么今天收到自己车保Geico
公司的的claim 信息。谁有类似经验的，请教了。谢谢！ |
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l*****6
发帖数: 752 | 43 请问谁用过American Express 行用卡的租车保险 （premium car rental protection
）claim 的。
租车出事故，以为是American Express是负责，不知道为什么今天收到自己车保Geico
公司的的claim 信息。谁有类似经验的，请教了。谢谢！ |
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h*******n
发帖数: 1328 | 44 我用过
你如果有premium car rental protection的话
应该是 American Express 赔的
不会用你自己的保险
你还车的时候自己没有向 american express claim吗？
你自己给租车公司geico的保险号的吗？
protection
Geico |
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y****g
发帖数: 685 | 45 I have a 20% off United Airline discount coupon for sale. Ask for $200,
non-negotiable! If interested, please call me at 312-929-8729. I live in
Chicago 60616. You can come to my house, book the ticket using the coupon
and then pay me. No worry about fraud! CASH ONLY! LOCAL ONLY! SERIOUS
BUYER
ONLY!
1. Usable for either domestic or international United or United Express
ticket purchase on United or United Express website.
2. Transferable
3. Expire by March 31st, 2013
4. Good for United First(R) ... 阅读全帖 |
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x**n
发帖数: 12542 | 46 我想要的物品:
American express AMEX points up to 50k
单张面值:
American express AMEX points 50000 or less
可接受的价格(必须明码标价！):
10k --- $100
20k --- $220
30k --- $350
50k --- $600
物品新旧要求:
new
邮寄方式要求:
internet transfer
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
Paypal or boa
其他补充说明:
广告的有效期:
物品来源:
我的联系方式:
mitbbs email
二手交易风险自负！请自行验证是否合法和一手卡！: |
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Q******i
发帖数: 551 | 47 二手交易风险自负！请自行验证是否合法和一手卡！:
y
我想卖的物品:
CVS,EXPRESS,old navy/GAP/BananaRepublic/Piperlime,JCPenney,RadioShack
单张面值:
25
可接受价格(必须明码标价！):
CVS/pharmacy® @25*6
EXPRESS @25*4
old navy/GAP/BananaRepublic/Piperlime @25*10
JCPenney @25*10
RadioShack @25*5
0.85+shipping
物品新旧要求:
new
邮寄方式要求:
YCYP or free code
买卖双方谁承担邮寄损失(Required if not code only):
before delivery, seller; after delivery, buyer
付款方式说明:
BOA or noncc paypal
其他补充说明:
自用之余出售闲置卡
广告的有效期:
Till gone
物品来源(Required for All Cards!):
DP
我的联系 |
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z*******2
发帖数: 658 | 49 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
Amerian express gc
单张面值:
500
可接受价格(必须明码标价！):
0.98
物品新旧要求:
New in hand
邮寄方式要求:
you choose, you pay
买卖双方谁承担邮寄损失(Required if not code only):
before delivery me, after delivery you
付款方式说明:
non-cc paypal
其他补充说明:
广告的有效期:
till gone
物品来源(Required for All Cards!):
Amerian express
我的联系方式:
bbs
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
yes, I will also give you my school email address and cell phone# for
contacting.
state and zip:
IL 60615 |
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p**x
发帖数: 6614 | 50 广告标题:
express换bloomingdales
我现有的物品:
express $100 ($25*4)
我想要的物品:
Bloomingdales (Same)
建议交换比率 (required):
1:1
邮寄方式要求:
各自负责，I will offer fcdc
买卖双方谁承担邮寄损失(required if not code only):
其它补充说明:
广告的有效期:
我的联系方式:
单张面值:
物品来源 (required for ALL cards!): |
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