由买买提看人间百态

topics

全部话题 - 话题: filtration
首页 上页 1 2 3 4 5 6 7 8 9 10 下页 末页 (共10页)
F***Q
发帖数: 6599
1

whole house water filtration主要是过滤sediment,我家有一个,是前屋主安装的
http://www.aquapure.com/aq-products/whole-house-filtration.html
http://www.aquapure.com/aq-products/whole-house-filtration/aqua
用的cartridge一般是过滤5um以上的粒子。不过换上cartridge一两各月打开还是能
trap很多脏东西。一般4-6个月换一次。
r********h
发帖数: 5638
2
来自主题: PennySaver版 - 闹心的净水器
Reverse osmosis (RO) is a water purification technology that uses a
semipermeable membrane. This membrane technology is not properly a
filtration method. In reverse osmosis, an applied pressure is used to
overcome osmotic pressure, a colligative property, that is driven by
chemical potential, a thermodynamic parameter. Reverse osmosis can remove
many types of molecules and ions from solutions, and is used in both
industrial processes and producing potable water. The result is the solute
is retai... 阅读全帖
i********e
发帖数: 50
3
来自主题: Biology版 - 请教native page

gel filtration出来的fraction还是需要别的方法来鉴定。原本是想native page的结
果比较可靠后用来检测gel filtration的fractions的。光从分光度来判断
gel filtration的fraction比较困难。1,蛋白质量少,很难有peak。2,单体蛋白就
550Kda,直接从bed volume就跑出来了。很怀疑跟4倍体能有retention time 上的显著
差异。
i********e
发帖数: 50
4
来自主题: Biology版 - 请教native page

faction,然后用这些fraction做western blot (注:这时候SDS-PAGE会让蛋白变性根
本就不重要,你只是要确定自己的蛋白是在哪些fraction里面)。gel filtration的每
个fraction根据和standard蛋白位置的比较,是可以知道大概的size的,那不就知道你
的protein的size了吗。你已知monomer是550Kda,到时候不就知道是monomer还是
tetramer了吗
我们的tetramer就是用gel filtration 分离纯化出来的,western 的结果也没问题。
现在怀疑monomer的存在。SDS-PAGE的话,假如原本是tetramer的蛋白也会变性成
monomer跑出来。这样就不知道原来fraction里的究竟是tetramer还是mononer.这就是
我为什么想用native-page的原因。monomer就有550Kda,一般gel filtration用的
standard 蛋白没有那么大的。最理想的结果是tetrmer在void volume,monomer不是。
用什么柱子能使2者的re
l**********1
发帖数: 5204
5
Please go to
//www.ncbi.nlm.nih.gov/pmc/articles/PMC2518226/
>Overall, our results suggest that PUB22 and PUB23 coordinately control a
drought signaling pathway by ubiquitinating cytosolic RPN12a in Arabidopsis.
Subcellular Localization
The sGFP cDNA clone was fused in-frame to the 59 end of the full-length
PUB22 and PUB23 coding region and ligated into the pBI221 transient
expression vector. The sGFP-PUB22 and sGFP-PUB23 fusion constructs
were transformed into protoplasts prepared from wild-typ... 阅读全帖
I********e
发帖数: 131
6
NJ大药厂,Job description见下,perfer Ph.D, master也考虑.有兴趣的话站内联系.
Scientist - Process Engineering Job Description:
The Scientist will involve conducting monoclonal antibody purification
process development to support the development, optimization and
manufacturing of biologics covering different therapeutic areas. Scope of
work may include, but are not limited to, protein purification, purification
process development, novel purification technologies, data analysis and
project reporting. The candidate i... 阅读全帖
M********r
发帖数: 179
7
问了几个地方,都觉得a 和e 都对,不知道是不是落下了什么重要的concept.
During an experiment, the following data are obtained from an anesthetized
dog:
Mean glomerular capillary hydrostatic pressure 45mmHg
Mean Bowman space hydrostatic pressure 12mm Hg
Mean glomerular capillary oncotic presure 23mmHg
Mean bowman space ontotic pressure 0mm Hg
Coefficient of filtration 3.0ml/
min mmHg
Renal blood flow ... 阅读全帖
I********e
发帖数: 131
8
【 以下文字转载自 Biology 讨论区 】
发信人: Islandtime (歇着呢), 信区: Biology
标 题: Contractor postion - Protein purifiecation process development
发信站: BBS 未名空间站 (Wed May 21 13:59:52 2014, 美东)
NJ大药厂,Job description见下,perfer Ph.D, master也考虑.有兴趣的话站内联系.
Scientist - Process Engineering Job Description:
The Scientist will involve conducting monoclonal antibody purification
process development to support the development, optimization and
manufacturing of biologics covering different therapeutic areas. Scope of
work may ... 阅读全帖
t***l
发帖数: 3644
9
当然和filtration有关,我同意如果用本身做filtration,那个是martingale。
但是具体到例子2,难道两个随机过程用不同的filtration来举例子?

be
W
but
t***l
发帖数: 3644
10
你觉得你说的这些我是不懂?太把自己当回事了吧。
这里又不是发paper,难道得一步一步写清楚。楼主说的显然是correlation process啊。
我当然知道correlation不关filtration什么事情。。。我的意思是你讨论两个BM,却
不用同一个filtration,你见过吗?举个例子看看?
你想想一个多维的BM,每个分量上的BM都用的同一个filtration吧,为啥?

.
(t
L**********u
发帖数: 194
11
来自主题: Quant版 - a stochastic calculus problem
谁说filtration是使得W(t)可测的最小sigma-algebra?
只有当filtration F(t)由W(t)生成的时候,这种特殊的filtration才是最小的。
两个概念,哥们不要混淆了哟,:-)
r**a
发帖数: 536
12
来自主题: Quant版 - a stochastic calculus problem
你说说你的filtration是啥?如果它大的吓人的话,那么你的结论我相信是对的。但是
这个filtration一般就是取那个最小的sigma代数吧。或者换句话说,你的结论在
filtration是那个最小sigma代数的时候不成立,这个你同意不?
z****n
发帖数: 1627
13

家用的反渗透净水系统,应该是先用MICRO FILTRATION或者NANO FILTRATION过滤,然
后可能会过一下活性炭,然后过反渗透膜,应该是不会用离子交换的。水烧开以后去除
的是暂时硬度,去不掉永久硬度。
t*******y
发帖数: 21396
14
Brita已经送去了,实在不行就RO呗。
https://www.brita.com/news/brita-filters-to-flint-mi
20,000 BRITA® FILTERS DISTRIBUTED TO FLINT, MI RESIDENTS AFTER HIGH
LEAD LEVELS FOUND IN WATER
Officials distributed 20,000 Brita® Faucet Filtration Systems to Flint,
MI residents after high levels of lead were found in the drinking water.
The state has declared the situation a public heath emergency, according to
the AP, and is encouraging residents in the area to filter their water.
The EPA warns that elevated ... 阅读全帖
L*****d
发帖数: 5093
15
https://www.huffingtonpost.com/rodales-organic-life/the-top-10-tapwater-
cities-in-america_b_8184138.html
The Top 10 Tap Water Cities in America
09/23/2015 12:29 pm ET Updated Dec 06, 2017
What makes a great glass of water? A pristine source, plus careful testing
and high-tech filtration and treatment.
by Michael Park, for Rodale’s Organic Life
New York City
More than 1 billion daily gallons of sweet, mineral-rich water travels over
125 miles from carefully guarded Catskill Mountains reservoirs.... 阅读全帖
T*******x
发帖数: 8565
16
来自主题: Military版 - 马尔可夫,martingale
我说一下我的一种理解。
martingale可以看成是,在越来越多的知识下,看同一个随机变量,得到的看法。也就
是说这个随机变量的物理没有变,只是看法在变。而看法的变化也要满足物理没有变这
个限制。
举个例子,比如测量恒星表面温度这个函数吧,它是恒星表面的每个点,的一个函数。
这个物理量假定它在那,不变。但是我们的测量手段有限。比如最开始,恒星在我们看
来就是一个点,我们虽然知道它不是一个点,但是没有手段分辨恒星表面是个面,所以
恒星表面温度这个函数,我们只能给它一个数,也就是表面所有点都是一个数。这个数
实际上也就是恒星表面温度的平均数。反过来看,这个数值对应的定义域,是恒星表面
所有的点,这也是这个集合上的唯一一个可测集,literally。
可以想象我们的测量手段越来越发展,也就是能够分辨或测量越来越细致的恒星表面温
度。比如在下一个阶段,我们有能力把恒星表面分辨出四个区域,并测量标注每个区域
的温度,那么恒星表面温度就是四个数,每个区域一个数,或者说每个区域里的所有点
给同一个数。可测集数量增加了,函数的标注细致了,平均值不能变。再下一个阶段,
分辨力测量手段越来越细致,可测集... 阅读全帖
y****i
发帖数: 2108
17
来自主题: Military版 - 口罩的常识
尼玛的还真是不见棺材不落泪。
戴好你的老花镜查查字典看清楚了。
https://www.fda.gov/medical-devices/personal-protective-equipment-infection-
control/masks-and-n95-respirators
N95 Respirators
An N95 respirator is a respiratory protective device designed to achieve a
very close facial fit and very efficient filtration of airborne particles.
The ‘N95’ designation means that when subjected to careful testing, the
respirator blocks at least 95 percent of very small (0.3 micron) test
particles. If properly fitted, the filtration cap... 阅读全帖
b*********h
发帖数: 1001
18
http://www.washingtontimes.com/news/2016/mar/29/paul-nehlen-cha
Wisconsin businessman Paul Nehlen announced Tuesday that he is the tea party
-allied candidate who is challenging House Speaker Paul D. Ryan in the
Republican primary, saying he’s “had it” with the speaker betraying
conservatives.
Sources first confirmed to The Washington Times earlier this week that a
wealthy businessman was mounting a primary challenge to Mr. Ryan in
Wisconsin’s 1st Congressional District, but Mr. Nehlen did not r... 阅读全帖
j*****y
发帖数: 121
19
Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical research
activities in China and expand its network to the scientific community. For
this purpose a new position is open for a dedicated and motivated Senior
Scientist/... 阅读全帖
s******4
发帖数: 1541
20
2009 Honda CR-V EX-L one owner Perfect condition
Mileage: 27,500
Engine: 2.4L I-4
Transmission: 5-speed AT
Ext. / Int. Color: Black / Black/Leather Seat Trim
Base MSRP in 2009: $28,245
Kbb Value: $23,400
Price Now: $21,000
Savings Off Total MSRP In 2009: $7,245
Warranty:
3 years 50,000 miles bumper to bumper
10 years 100,000 miles power-train
STANDARD EQUIPMENT
ABS - 4-wheel
Air filtration - Interior air filtration
Driver Airbag - Yes, Antenna Type - Integrated roof
Braking assist - Braking assi... 阅读全帖
j*****y
发帖数: 121
21
Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical research
activities in China and expand its network to the scientific community. For
this purpose a new position is open for a dedicated and motivated Senior
Scientist/... 阅读全帖
n******t
发帖数: 367
22
1) Central Vacuum $ 1950
家里有的同学讲讲有需装这个吗? !
2) Water filtration system
2.a.Reverse Osmosis system to produce bottled quality water ($525)
2.b. Water filtration ($450) to remove sediment, odors, taste.
还是直接去Costco买那种过滤的自己装?
3)在卧室和卫生间装个门 $ 500
不知道是不是不要门更开阔些?
4)楼下地板 选择木地板还是好点的防滑的地砖?
多谢了
g********t
发帖数: 515
23
来自主题: Living版 - 你们家里灰尘多吗
吸尘的同时如果吸尘器不好同时也把灰尘吹出去
一般要买带HEPA filtration的吸尘器,但是很多厂家HEPA filtration做的很差,选好
点的seal就不会有灰尘吹出来
e****b
发帖数: 14
24
来自主题: Living版 - HELP! Replace central AC system.
我们在boston, 房子现有的central AC太旧了,准备换套新的,quote了几个Bryant和
Lennox的dealer,都推荐用heat pump, 这样可以有hybrid heating (我们取暖是用gas
boiler).
版上的大侠给点意见吧,Bryant和Lennox都是不错的牌子,从cost和reliability来说
,哪个quote比较reasonable? 多谢了.
1. Extreme Evolution Hybrid Heat System 280ANV with Perfect Humidity
variable speed fan coil and Wifi I touch control.
Total $16000
2. Extreme Evolution Hybrid Heat System 286BNA with Perfect Humidity
variable speed fan coil and Wifi I touch control.
Total $14000
3.Lennox airhandler CBX40UHV-036 an... 阅读全帖
y****o
发帖数: 1478
25
re, 感觉好像只能买那种
Brita Water Filtration System
比如:
http://www.drugstore.com/brita-water-filtration-system-complete
w*********9
发帖数: 120
26
来自主题: PennySaver版 - Brita Water Filter 到底有没有用?
Not for sure, but my guess spring water is made of regular groundwater -->
sand filtration --> Granular activated carbon filtration --> ultrafiltration
/ nanofiltration --> UV disinfection. Maybe add something to adjust the
taste. Spring water tastes a little better than purified water because lots
of dissolved ions are reserved in the water without being removed like
purified water, which give you a feeling of "natural taste".
r****y
发帖数: 263
27
收入推荐信模板类别http://user.qzone.qq.com/2915799706/blog/1398826316
Angellee推荐信1
U.S. Departmentof Homeland Security
Feb 19, 2013 Citizenship and Immigration
Services
RE: OpinionLetter to SupportI-140 Immigrant Visa Application Applicant:
Angellee, Ph.D.
Fields of Expertise: Chemical Biology, Antibody Engineering, Protein
Therapeutics, Nanobiotechnology, and Cancer Therapy
Dear Sir/Madam:
I am writing this le... 阅读全帖
l*****g
发帖数: 556
28

really good market then. Folks from big water filtration company thought
there is no need for water filtration in USA. They are wrong...
f****a
发帖数: 4708
29
来自主题: SanFrancisco版 - West San Jose 的水
我们家用的是这个,
Premier Reverse Osmosis Water Filtration WP-5
http://www.costco.com/Premier-Reverse-Osmosis-Water-Filtration-
加装了
Watts Premier 560060 pH Balancing Ceramic Filter
http://www.amazon.com/gp/product/B005ILIM7Y
每年更换filter pack的费用是35块。半透膜3-5年换一次,每次45块。
w******n
发帖数: 12
30
来自主题: Texas版 - houston downtown附近 moving sale
联系email: m********************[email protected]
我在midtown附近,离houston downtown不远
下面链接仅供参考,实物照片稍后上传
还有一堆厨房用锅碗瓢盆未列出

1. king size床,$350,(包括frame,box,pillowtop mattress,mattress pad
),我买的是全新的,用了不到2年,大部分时间只是一个人睡)
2. luxury love seat,$200,(买来时全新,用了大约1年,标签还在,原价$954)
3. dell电脑,dimension E520 主机,$120,(inter(R) core(TM)2 cpu, 4300@1
.8GHz,1GB内存,250G硬盘)
4. 三星syncmaster 226BW 22-inch LCD Monitor,$150(2ms反应速度,高性能显
示效果)
5. JBL Creature II 3-Piece Powered Speaker System $40 http://www.amazon.... 阅读全帖
s*****i
发帖数: 5548
31
☆─────────────────────────────────────☆
xie91 (bob) 于 (Mon Mar 4 19:12:18 2013, 美东) 提到:
发信人: kylin1996 (kylin), 信区: SanFrancisco
标 题: Re: 再谈德州经验 (转载)
发信站: BBS 未名空间站 (Mon Mar 4 17:16:16 2013, 美东)
看见有人说湾区收入高就笑了,给你们科普一下,猴子屯,13-15w年薪非常普通,20w
以上的一大把,加上公司100% match 6-12% 401k, 公司服务到退休有一次性上百万的
pension,还有一周工作四天一周工作五天,每年5周vacation,加州风景再美,这些能
比吗?
☆─────────────────────────────────────☆
delubo (I shall be your eyes!) 于 (Mon Mar 4 19:13:42 2013, 美东) 提到:
求介绍工作,我要跳槽
☆────────────────────────────... 阅读全帖
b*****l
发帖数: 8603
32
IRON 30-160 UG/DL 13
TOTAL IRON BINDING CAPACITY 228-428 UG/DL 448
IRON/TOTAL IRON BINDING CAPACITY 13-50 % 3
FERRITIN 10-150 NG/ML 3.0
WBC'S AUTO 3.5-11.0 K/UL 3.7
RBC, AUTO 3.5-5.30 MIL 4.30
HGB 10.4-15.3 G/DL 8.3
HEMATOCRIT 33-46 % 28.0
MCV 73-101 CMICRON 65.2
MCH 22-33 PG 19.3
MCHC 30-36 GM/DL 29.7
RDW, BLOOD 12.0-17.2 % 20.5
PLATELETS, AUTOMATED COUNT 130-385 K/UL 330
MPV 6.8-10.4 FL 9.3
HEMOGLOBIN A,BLD,QN, ELECTROPHORESIS >96 % 97.8
HEMOGLOBIN A2, BLOOD, QUAL 1.8-3.5 % 2.2
GLUCOSE POST FA... 阅读全帖
s***e
发帖数: 5242
33
(谁说养鱼不算才艺?)
我最喜欢的动物是狗,所以养了最接近狗的宠物。
金鱼。
是的,金鱼温柔敦厚,见人摇尾巴,从人手里吃食,有的任人抚摸,能活十几年。
形态各异,颜色丰富,憨态可掬。
而且维护简单,就是每周换水,每月换滤片,有时间陪它们就行。
养鱼其实不久,家有三条分别叫 Bumblebee the Common, Ladybug the Fantail, Nasa
the Oranda,为儿子女儿我在另一个空间的投影。斗胆分享下拥有身心健康的金鱼的
十条撇步。
1. fish tank
一寸鱼两加仑缸。除去尾巴,如果一条鱼2寸,三条鱼的话就12加仑,不能再小了。因
为鱼会根据缸的大小控制自己的大小,但内脏会持续成熟,缸太小鱼就撑死了。如果不
想鱼被束缚在狭小的空间里,就象鸡场里的可怜的鸡,鱼缸得是3倍鱼长的宽,2倍鱼长
的厚,1.5倍鱼长的高,那么就是9倍的鱼长的立方。成熟的鱼大概会8寸到12寸,所以
40加仑左右的缸大概是必须的。水缸越大,水质越稳定,越容易保持鱼健康。
2. cycle a fish tank
鱼产生的废物中,ammonia是致命的,幸运的是,无所不在的细菌会把它... 阅读全帖
x**0
发帖数: 6149
34
【 以下文字转载自 pets 讨论区 】
发信人: x100 (文科僧+康泰时轮), 信区: pets
标 题: 请教一下用过rabbit air purifier的同学们
发信站: BBS 未名空间站 (Mon Jul 4 16:19:16 2011, 美东)
想买一个rabbit air purifier,有两个系列的,不知道应该买哪种,给指点一下吧,
多谢。
一种是Rabbit Air MinusA2 Air Purifier
还有一种是Rabbit Air BioGS
第一种贵一些,5 stage filtration,第二种便宜一些,3 stage filtration。
如果主要用来过滤pet allergen (cat dander)哪种更合适?或者两者功效差不多?
还有这个filter大概多久要换一次?
x**0
发帖数: 6149
35
想买一个rabbit air purifier,有两个系列的,不知道应该买哪种,给指点一下吧,
多谢。
一种是Rabbit Air MinusA2 Air Purifier
还有一种是Rabbit Air BioGS
第一种贵一些,5 stage filtration,第二种便宜一些,3 stage filtration。
如果主要用来过滤pet allergen (cat dander)哪种更合适?或者两者功效差不多?
还有这个filter大概多久要换一次?
s***e
发帖数: 5242
36
来自主题: pets版 - 金鱼养殖经验
感谢分享。
你说的确实是主流看法,但楼主是古法养鱼(缺少植物部分),虽然我不赞同,但结果
貌似还行。
最大的疑问是nitrate和排泄物的处理,浓度都非常高,即使水看起来清澈,但其实不
是特别健康。就象帝都雾霾成这样,还是几千万人生活着。
关于鱼缸和filtration的大小,你说的是最小值。一条10寸的鱼,如果只是10加仑的缸
,大概就10*20*10,基本就游不起来,跟养殖场的鸡差不多。所以,最好1寸鱼2加仑缸
。而且filtration要10倍,就是10加仑的缸,要每小时过滤100加仑的水。
关于换水,你说不能频繁,不能超过30%,其实只说对了一半。就是在水质一般的情况
下,换水过多会导致水质变化,从而shock鱼。但如果水质非常好,水里除了少量
nitrate没有别的,那其实只要注意温度变化要小外,换90%都是可以的。一些大拿这么
做,但是一般爱好者还是每周20%比较稳妥。
还有纯鱼缸,就是裸缸,个人觉得也不是最好。金鱼喜欢sift,建议铺一薄层重沙(比
如Tahitian Moon Sand),或者有些algae供其消遣。真的植物我觉得是必须的,鹅卵
石小假山通通不要。
s***e
发帖数: 5242
37
【 以下文字转载自 LeisureTime 讨论区 】
发信人: sukye (勤奋的小苏●书鱼), 信区: LeisureTime
标 题: 【 2015】一个fresh goldfish farmer的情感十问
发信站: BBS 未名空间站 (Mon Dec 14 09:20:14 2015, 美东)
(谁说养鱼不算才艺?)
我最喜欢的动物是狗,所以养了最接近狗的宠物。
金鱼。
是的,金鱼温柔敦厚,见人摇尾巴,从人手里吃食,有的任人抚摸,能活十几年。
形态各异,颜色丰富,憨态可掬。
而且维护简单,就是每周换水,每月换滤片,有时间陪它们就行。
养鱼其实不久,家有三条分别叫 Bumblebee the Common, Ladybug the Fantail, Nasa
the Oranda,为儿子女儿我在另一个空间的投影。斗胆分享下拥有身心健康的金鱼的
十条撇步。
1. fish tank
一寸鱼两加仑缸。除去尾巴,如果一条鱼2寸,三条鱼的话就12加仑,不能再小了。因
为鱼会根据缸的大小控制自己的大小,但内脏会持续成熟,缸太小鱼就撑死了。如果不
想鱼被束缚在狭小的空间里,就象鸡场里的... 阅读全帖
r******y
发帖数: 3838
38
http://www.macrumors.com/2011/01/14/ios-4-3-beta-hints-at-openc
devices/
When it comes to the graphics performance on Apple's iOS devices, the
company has been sticking with the
powerful POWERVR SGX535 GPU from Imagination Technologies for quite some
time, introducing it on the
iPhone 3GS and carrying it through into the company's own A4 system-on-chip
designs for the brains of the
iPad and iPhone 4. But hints included in the new iOS 4.3 beta seeded to
developers earlier this week indicate
that ... 阅读全帖
r******y
发帖数: 3838
39
【 以下文字转载自 Apple 讨论区 】
发信人: rhapsody ( input type=\"button\" value=), 信区: Apple
标 题: iOS 4.3 Beta Hints at OpenCL-Capable SGX543 GPU in Future Devices
发信站: BBS 未名空间站 (Tue Mar 8 19:46:52 2011, 美东)
http://www.macrumors.com/2011/01/14/ios-4-3-beta-hints-at-openc
devices/
When it comes to the graphics performance on Apple's iOS devices, the
company has been sticking with the
powerful POWERVR SGX535 GPU from Imagination Technologies for quite some
time, introducing it on the
iPhone 3GS and carrying ... 阅读全帖
n****e
发帖数: 1677
40
来自主题: Biology版 - Re: 蛋白纯化的脱盐问题
The salt concentration after the gel filtration should be the same as the
running buffer. I usually use 100mM NaCl to run gel filtration, it depends on
the protein.
I don't know what you gonna do to store the protein you purified. I usually
concentrate the protein down to 10mg/ml, and save it for crystallization.
I save the protein in 5% Glycerol, 10mM DTT, 100mM NaCl and 20mM whatever
buffer. So I usually make the buffer, concentrate the protein down to a
reasonablly small volume, add the buffe
s******y
发帖数: 28562
41
You should NOT use the gel filtration because it will significantly dilute
your sample.
If you have a doubt about this, you should load the eluant directly without
any pulldown to see how much full length protein you recovered.
I don't know why you are bothered by the free amino acid. They will be
washed out in the pulldown process. I suggest you NOT to do the gel
filtration at all.
What you need to do is this:
1.centrifuge to pellet the ribosome. Are you sure you are using 100,000 rpm?
Not 100,
s******y
发帖数: 28562
42
I remember seeing some 50mL membrane filtration units around.
They are inverted, that means the total solution is put in the bottom of the
unit, and the filtrate will flow up to the reception unit that is inserted
inside the tube.
For such setting, it won't be clogged by lysate.
a****k
发帖数: 1130
43
来自主题: Biology版 - 请教native page
就是说,你拿自己的sample run一个gel filtration column,collect每一个faction,然后用这些fraction做western blot (注:这时候SDS-PAGE会让蛋白变性根本就不重要,你只是要确定自己的蛋白是在哪些fraction里面)。gel filtration的每个fraction根据和standard蛋白位置的比较,是可以知道大概的size的,那不就知道你的protein的size了吗。你已知monomer是550Kda,到时候不就知道是monomer还是tetramer了吗

gel
s******s
发帖数: 13035
44
直接用millipore的filtration column, 完全物理size filtration,和
binding没关系。
http://www.millipore.com/catalogue/module/c82301
有不同size cutoff,也有不同的volumn (比如15ml, 4ml)
j*****y
发帖数: 121
45
Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical research
activities in China and expand its network to the scientific community. For
this purpose a new position is open for a dedicated and motivated Senior
Scientist/... 阅读全帖
I*********r
发帖数: 8
46
我不确定楼主的意思。你是想知道有活性蛋白是否是dimer?
如果你可以提纯蛋白,那做一个gel filtration,看看活性蛋白的大小,推测是否为
dimer。如果gel filtration能同时得到dimer和monomer,分别测定它们的活性啊。
或者是我理解错了?

dimer
j*****y
发帖数: 121
47
发信人: jeffsky (半夏决明子), 信区: Pharmaceutical
标 题: 代友发:Scientist in DSP in Novo Nordisk R&D Center China
关键字: 生物制药 工作 回国 求职
发信站: BBS 未名空间站 (Wed Aug 21 19:07:21 2013, 美东)
Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical re... 阅读全帖
Q***5
发帖数: 994
48
But you need to change the filtration first. w(t) is not even adaptive to
the filtration of b(t), let along a martingale.
It seems that this problem be proved directly by definition of B.M.
Q***5
发帖数: 994
49
But the t to 1 part involves `information' that is not available at t in the
original filtration. -- for example, there should be a b(1) term from the t
to 1 integration.
If I understand the question correctly, we need to prove that w(t) is B.M.
with respect to filtration \sigma (w(s), s<=t), not \sigma(b(s), s<=t).
Since the process is a Gaussian process, we only need to check the
independent incremental property and the std of w(t_2)-w(t_1), using the 2
part integration you mentioned.
n******t
发帖数: 4406
50

the
t
~~~~~~~~~~~~~~~~~~~~
Yes, we can not use the filtration generated by b(t), but I think
in this case there is no problem to enlarge the filtration.
Basically, if Let the first part be X(t) and the second part be Y(t),
then from the definition, we could see that we can actually add
their QVs together which gives out t.
首页 上页 1 2 3 4 5 6 7 8 9 10 下页 末页 (共10页)