G********e
发帖数: 528 | 1 I'd like to ask if it is possible to do so.
For example, PFA fixation, FACS sorting for specific cell population, then
lyse protein for western.
The reason not doing live cell FACS is lack of the cell surface marker for
the specific cells I'm interested in. I have to do fixation/permeablization
and intracellular marker for cell purification. But after that, it seems
very hard to get protein extract from fixed cells. Can I use mild fixation?
Please let me know your experience, if any. |
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s****9
发帖数: 932 | 2 Thanks. I will have a try of PFA fixation with protein retrieval.
The purpose of the IHC is to in situ visualize the cell type which has the
enzyme expression(located on the ER membrane). My laser capture data
suggests that the enzyme selectively expressed on certain regions but that
regions contains many cell types. IP is not useful for me.
My concern is that the epitope is intramembrane and thus cannot expose out
after fixation.
t
if |
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T**********t
发帖数: 1604 | 3 主要是让蛋白变性沉积，跟covalent bond没啥关系。
http://en.wikipedia.org/wiki/Fixation_%28histology%29
Precipitating fixatives - Alcohols
Precipitating (or denaturing) fixatives act by reducing the solubility of
protein molecules and (often) by disrupting the hydrophobic interactions
that give many proteins their tertiary structure. The precipitation and
aggregation of proteins is a very different process from the crosslinking
that occurs with the aldehyde fixatives.
The most common precipitating fixatives are et... 阅读全帖 |
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b*****l
发帖数: 9499 | 4 过去做 treatment 取不同时间点时，用冷 medium 叫停细胞，然后做 RPPA。最近琢磨
着，能不能用可逆的 fixation 来叫停细胞啊？比如说，要是用冷的 methanol 啥的在
某个时间点把细胞 fix，然后在 methanol 溶液中洗啊离心的，再放到啥溶液里把细胞
lyse 了，同时让被 fix 的 protein 都恢复自由，不知道成不成？哪位试过或者见过
相关的 literature 不？ |
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y****6
发帖数: 196 | 5 HEK293 cells in 96-well plate, DMEM with 10% FBS, cultured 2d, about 100%
confluency.
Washed once with PBS before adding fixation solution (3.7% formaldehyde/PBS).
Cells detached in fixastion solution. Is it because formaldehyde does not
work with HEK293 cells? Anyone tried methenol? What's the concentration? |
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y****6
发帖数: 196 | 6 Can anyone explain how fixation works? My understanding is that fixative
helps the crosslink of proteins and other macromolecules. I also have an
impression that it helps the cells to attach to the plate more firmly. It is
correct? |
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h****n
发帖数: 2552 | 7 Fixation reagent is no use to help cells attch on a plate. I suggest coating
your
plate with poly-lysine
is |
|
s****9
发帖数: 932 | 8 IHC stain一个ER上膜蛋白，自己做的多克隆抗体（rabbit），western效果很好，size
和报道的一直，KO tissue没有带。 但是IHC一直不成功，IHC用的是frozen tissue，
acetone fixation，fast blue development。
想请教:
１。这种ER上的膜蛋白用什么方法fix比较好。我用cold acetone 5min，也试过一次4
％PFA。似乎效果都不好，和KO没有区别。
２。有什么方法可以提高信号强度。有成熟的tyramide protocol可以提供吗? |
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h*******o
发帖数: 4884 | 9 Are you sure it is the fixation problem?
Have you tried with antigen retrieval. |
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s****9
发帖数: 932 | 10 I understand it can be the problem of the antibody and will never be solved,
but want to try as hard as possible.
Could you suggest a few antigen retrieval method? Do you think 5min acetone
fixation is enough to expose the antigen epitope?
Very few background on the staining. Do you think it is worthy to try
different blocking reagents?
Thanks so much. Really appreciate your suggestions.
or |
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c******t
发帖数: 1733 | 11 Common errors performing classic lifts.
by Sergey Rudnev,IKSFA Master Coach
a) JERK 1. Error: feet are wider than shoulder width Consequences: reduction
of the distance and speed of acceleration of the bells, worsening of the
efficiency of the leg work. Causes: low mobility in the hip joints or lack
of knowledge of physics. Correction: in the former case I recommend rocking
in gymnastic bridge or static rack holds (1-5 minutes). In the latter I
recommend revising the section of physics entitled ... 阅读全帖 |
|
c******t
发帖数: 1733 | 12 Common errors performing classic lifts.
by Sergey Rudnev,IKSFA Master Coach
a) JERK 1. Error: feet are wider than shoulder width Consequences: reduction
of the distance and speed of acceleration of the bells, worsening of the
efficiency of the leg work. Causes: low mobility in the hip joints or lack
of knowledge of physics. Correction: in the former case I recommend rocking
in gymnastic bridge or static rack holds (1-5 minutes). In the latter I
recommend revising the section of physics entitled ... 阅读全帖 |
|
b*********b
发帖数: 64 | 13 如果你很想了解confocal或者更高级的two-photon，下面两篇paper对你会有帮助。
1.Ojcius DM et al.Immunology and the confocal microscope. Res Immunol 1996
2.Cahalan MD et al.Two-photon tissue imaging: seeing the immune system in a
fresh light.Nat Rev Immunol 2002
ZL1999 (胖头猫)提到了confocal和一般fluoresent microscopy的区别，除了有一点我
不太认同，根据我的理解，confocal microscopy的photobleaching问题应该比一般的
fluoresent microscopy要小，因为前者illuminate smaller volume of your sample。
如果你要做的是培养的细胞，你可以不用改protocal，我一般用8-well chamber slide
，这样一张slide上可以有8个samples。coversl... 阅读全帖 |
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r******0
发帖数: 357 | 14 BMC终于在最近做了一件值得尊敬的事情。我敢说下面的狗屎99%都是所谓的个性化生物
“信息”“润色”的畸形怪胎。一般以所谓的Meta分析或者随便下载一套狗屎基因芯片
数据，然后做一些似是而非的烂分析，发一堆垃圾，一篇文章卖给忙于召妓的医生5万
到10万。
1.
Retraction Note Open Access
Retraction Note: Impact of guidewire selection and operator expertise on
radiation exposure in transradial angiography
Jianmin Yang, Ningfu Wang, Xiaoshan Tong, Xianhua Ye, Liang Zhou, Guoxin
Tong, Yun Shen, Shuzheng Lv
Journal of Cardiothoracic Surgery 2015, 10:42 (27 March 2015)
Full text | PDF
2.
Retraction Note Open Access
Retracti... 阅读全帖 |
|
z****g
发帖数: 3340 | 15 做ChIP有些年头了，最近遇到一个新问题。以前都是细胞在板上时做Fixation，然后从
板上刮下来做后续处理，一直很好。最近做一个很难从板上刮下来的细胞，就先用
Trypsin消化之后，把细胞放在大试管里象悬浮细胞那样做Fixation，然后离心作
sonication。但是好像结果不太好,会不会实验的顺序或者在试管里做Fixation不好.有
经验给说说. |
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e****0
发帖数: 678 | 16 • Exaggerated deep tendon reflexes can be seen in lock in syndrome.
• Non bleeding varices are managed with nonselective beta-
adrenergic antagonists, such as propranolol.
• Sclerotherapy, endoscopic band ligation, and surgery are
indicated after a patient has a first episode of variceal bleeding.
• TIPS is a last resort in variceal bleeding unresponsive to
medical and endoscopic intervention.
• Ischemic colitis
CT—thickening of eth bowel ... 阅读全帖 |
|
G*******n
发帖数: 6889 | 17 krauts帮NSA偷窥高卢鸡，高卢鸡偷偷联络krauts反对党领导人
http://www.wsj.com/articles/hollande-calls-defense-cabinet-meet
PARIS—French President François Hollande plans to convene top defense
advisers on Wednesday after WikiLeaks published documents that purportedly
show that the U.S. spied on him and his two predecessors.
The six documents, also published late Tuesday by two French publications,
describe purported U.S. surveillance of internal deliberations and
conversations of Mr. Hollande and former French pr... 阅读全帖 |
|
y*****g
发帖数: 193 | 18 The doctor did nothing wrong. Aganist medical advice,you can't win the
case.
Either internal fixation or external fixation of those fracture pts I saw is
all done in OR, no reduction done preoperational at bedside.
It is the sad case, however, the doctor should not be the person to be
blamed. |
|
z*****k
发帖数: 600 | 19 I don't feel this thing is such a big deal. But I am not so much into
following any particular pros' styles either. I am not sure why you'd be
serious with this head thing. Maybe you like to improve some fundamentals?
Just my impression: this "head fixation" stuff is a side-effect of forming a
rotating axis between your head and one of your feet (depending on natural
or open stance), before starting shoulder rotating/whipping your arm forward
. Once this axis framing is there, i just don't see w... 阅读全帖 |
|
n******8
发帖数: 558 | 20 Song
Christina Rossetti
When I am dead, my dearest,
Sing no sad songs for me;
Plant thou no roses at my head,
Nor shady cypress tree:
Be the green grass above me
With showers and dewdrops wet;
And if thou wilt, remember,
And if thou wilt, forget.
I shall not see the shadows,
I shall not feel the rain;
I shall not hear the nightingale
Sing on, as if in pain:
And dreaming through the twilight
That doth not rise nor set,
Haply I may remember,
And haply may forget.
歌
我死去时， 最亲爱的你，
请不要... 阅读全帖 |
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P******l
发帖数: 1648 | 21 本文转载自最新一期的使者杂志
http://tiny.cc/20170304-am-evolution
当科学与信仰交集时——进化论是科学吗？ /张纪德
许多基督徒及慕道友对科学与信仰方面的讨论感兴趣，但要小心一些似是而非的认知。
使者杂志59|6期的《生命演化与基督信仰》一文，笔者觉得有诸多可商榷之处。比如说
:「演化本身有充分科学证据的生物机制」(p 48 右上）；又说，「从最简单的形式开
始……上帝掌管生命演化的机制……」(p 49 左）等，均不恰当。
或许有些人不清楚，演化论就是进化论（Evolution）， 与达尔文主义（Darwinism）
同义。在一般教科书及报导文章里都把进化论当科学；但是，各种生物是进化来的吗？
生物在演化吗？
历史沿革
自1859 年达尔文发表《物种论》以来，进化论对科学及宗教信仰起了很大的影响。许
多知识分子全盘接受达尔文的进化论，以至达尔文主义成为一种世俗的意识形态，与宗
教信仰有很大抵触。
近百年来，有部分神学家在解经上妥协，以进化论的框架来解释圣经，发展出各样版本
的 「神导进化论」新派神学。
多数的神学家、牧师一再努力抗拒新派神学，但... 阅读全帖 |
|
p*****n
发帖数: 981 | 22 ☆─────────────────────────────────────☆
chick (Por Los Momentos Dif Ciles) 于 (Wed Jan 3 13:21:18 2007) 提到:
I'm working on the project involved something similar to Ch-IP. Recently, I
got very weird result, in some group or controls, the Non-fixation IP has
even stronger singal than the formaldehyde fixation. This could partialy be
explained by the over-crosslinking lowered the possible detecable targets.
But, obviously, I need more stringent washing condition, at least to
diminish the non- |
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b******s
发帖数: 1089 | 23 Sorry for English. Can't input chinese in the lab.
Nowadays, people choose to fix samples by high pressure freezing and freeze
substitution more and more. There is no question that this method is elegant
to keep the cellular structure and keeping the antigen active for immuno-EM
.
I read many discussions in EM mail group talking about their papers got
rejected because they used the conventional chemical fixations. It seems
people in EM field have different opinions about requirement of using hi... 阅读全帖 |
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l*h
发帖数: 4124 | 24 yes, you can. but uneven fixation in whole mount is common. if your antibody
is sensitive to fixation depth, the results can be bad though. |
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m*******r
发帖数: 202 | 25 Cell Cycle. 2005 Mar;4(3):453-5. Epub 2005 Mar 7.
Cell cycle markers for live cell analyses.
Easwaran HP, Leonhardt H, Cardoso MC.
SourceMax Delbrück Center for Molecular Medicine, Berlin, Germany.
Abstract
Many cellular processes are regulated by cell cycle dependent changes in
protein dynamics and localization. Studying these changes in vivo requires
methods to distinguish the different cell cycle stages. Here we demonstrate
the use of DNA Ligase I fused to DsRed1 as an in situ marker to ident... 阅读全帖 |
|
|
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a*********n
发帖数: 2526 | 28 我想探测一个protein 是不是colocalize with gamma-tubulin at centrosome.
发现如果用methanol fixation可以检测到protein在centrosome上，但是用PFA
fixation 就检测不到centrosome上的信号。
不知道应该相信哪一个结果？
谢谢1 |
|
g*********e
发帖数: 150 | 29 Formaldehyde, glutaraldehyde, alcohol, etc. does not "quench" fluorescence.
Instead, it denatures the GFP such that it no longer functions and
fluoresces. Any remaining fluorescence is either non-specific (many proteins
auto-fluoresce after fixation) or it is a few GFP molecules that retain
their original conformation. If you fix the tissue, then as Ann Cantereau
states, you MUST use an anti-GFP antibody to detect the GFP. At that point,
you are "seeing" the GFP by whatever tag you have on the s... 阅读全帖 |
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p**a
发帖数: 28 | 30 来自主题: MedicalCareer版 - 紧急求助 This is a proximal ulna fracture with radial head anterior dislocation, also
called Monteggia fracture type 1. The
young surgeon is correct, do not believe a doctor just because he is old.
However for treatment, try closed reduction with cast fixation first. In
your case, since it is 5 days out, it could
be difficult for closed reduction, but worth a try. If it cannot be reduced
by manuver, then surgical open
reduction and internal fixation is indicated. |
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p**a
发帖数: 28 | 31 来自主题: MedicalCareer版 - 紧急求助 It does not mean anything having no pain in the cast. The radial head MUST
be reduced, either closedly or
surgically. To maintain the reduction, the ulna fracture MUST be reduced and
stabilized, either with cast or
internal fixation. Probably the surgeon feels that it would be very
difficult for close reduction and cast fixation.
In this case, surgery is in your best interest.
No matter what you choose, close reducton or surgery, the earlier the better
. Do not wait too long with wishful
thinkin... 阅读全帖 |
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b****n
发帖数: 90 | 32 昨天晚上万分焦急中在这里发贴求助,询问我5岁女儿手臂骨折加脱臼的事,得到几位热心的朋友的专业答复.虽然人在异乡,我们从未谋面,但你们的热心帮助让彷徨无助的我们有了making decision的勇气.这里要特别感谢
Aplusplus (Hakuna Matata)
Lexian (蒙古大夫)
NoDreamer (锦衣刀客)
phxa (sutro)
多谢版上几位好心人的专业教导,昨晚半夜之前我们就基本上决定了听从第二个大夫的意见做手术,但准备要求大夫先try closed reduction.后来国内一骨科大夫的意见反馈回来了,他的意见和版上几位专家的意见也差不多,也说桡骨有不明显的移位.只是说在中国这种情况基本上都是closed reduction.至于是否需要internal fixation他说他不确定,因为他没看到打了石膏的x光片.
在等待国内骨科大夫回音的那段时间里,查了一下第一个"骨科大夫"的record,结果大吃一惊:这位根本就不是什么骨科大夫,而是一个pediatric emergency doctor!出事当天女儿的pediatrician先是让我带小孩回家,说第... 阅读全帖 |
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N*******r
发帖数: 118 | 33 有桡骨头脱位就必须复位（Reduction), 复位一般有两种：Open reduction and
closed reduction。Open reduction是手术切开后在X光下复位，Closed reduction就
是不切开皮肤组织，单靠手法在X光下复位。复位后还要固定，固定也分内固定（Internal
fixation)和外固定(External fixation)。内固定是使用钢板螺钉....，而外固定一般是指
石膏夹板。
实际上对一个5岁的孩子来讲，不管是Closed reduction还是Open reduction，都可说
是手术，也都需要全麻。具体要用那种方法，要看具体情况，只能由她的医生来定。
只不过根据她的X光来看，骨折移位脱位都不明显，不知是否可先试一下Closed
reduction，你可以明天早晨和医生再探讨一下。 |
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b****n
发帖数: 90 | 34 顶起来.
热心的朋友的专业答复.虽然人在异乡,我们从未谋面,但你们的热心帮助让彷徨无助的
我们有了making decision的勇气.这里要特别感谢
的意见做手术,但准备要求大夫先try closed reduction.后来国内一骨科大夫的意见反
馈回来了,他的意见和版上几位专家的意见也差不多,也说桡骨有不明显的移位.只是说
在中国这种情况基本上都是closed reduction.至于是否需要internal fixation他说他
不确定,因为他没看到打了石膏的x光片.
吃一惊:这位根本就不是什么骨科大夫,而是一个pediatric emergency doctor!出事当
天女儿的pediatrician先是让我带小孩回家,说第二天会帮我们联系一位骨科大夫.回家
途中接到电话说骨折还是不应该等,已联系好了Children's Hospital一位orthopedist,
让我紧急送女儿到Children's Hospital ER去见该大夫.搞了半天我们一致把李鬼当李
逵.我们也不知道这是在那个环节出了问题,除了有点气愤外,更多的是安心,知道今天该
怎么办了.
closed... 阅读全帖 |
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A*******s
发帖数: 9638 | 35 Thanks for the updates! We are so happy that your daughter avoided being cut.
热心的朋友的专业答复.虽然人在异乡,我们从未谋面,但你们的热心帮助让彷徨无助的
我们有了making decision的勇气.这里要特别感谢
的意见做手术,但准备要求大夫先try closed reduction.后来国内一骨科大夫的意见反
馈回来了,他的意见和版上几位专家的意见也差不多,也说桡骨有不明显的移位.只是说
在中国这种情况基本上都是closed reduction.至于是否需要internal fixation他说他
不确定,因为他没看到打了石膏的x光片.
吃一惊:这位根本就不是什么骨科大夫,而是一个pediatric emergency doctor!出事当
天女儿的pediatrician先是让我带小孩回家,说第二天会帮我们联系一位骨科大夫.回家
途中接到电话说骨折还是不应该等,已联系好了Children's Hospital一位orthopedist,
让我紧急送女儿到Children's Hospital ER... 阅读全帖 |
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q********g
发帖数: 10694 | 36 第一部分：选题与创新
一、先想先写最后做:
1. 做研究之前，必须想清楚：结果能不能发表？发表在哪里？
2. 先把文章大框写好，空出数据，等做完实验填完空就可以发了；正所谓心中有沟壑！
3. 在未搞清“写什么、发哪里、自己研究与同类研究有何出色之处”之前，就不要动
手做！
4. 继续去看文献，去想；想不清楚就做还不如不做！
5. 要想这样做，就得先看文献！要知道如何把文章架起来、要知道别人是如何讨论的
、要知道自己的数据是不是说明了与别人不同的东东或别人没有做过……这个过程就是
阅读文献及思考的过程，这些搞清楚了，写就简单了！
6. 要是先做事，做完发现别人做过，或无法用理论来解释，岂不是冤大头？
二、如何科学选题:
1. 课题选择和国际接轨。想在国际核心期刊发表文献，就必须了解国际研究动态
，选择与国际学术研究合拍的课题。由于多方面因素的影响，我国科学研究选题与国际
先进水平还有一定距离。我国一家权威科研机构不久前在国内挑选了许多前沿领域的研
究课题，准备参与国际合作，但到美国后发现近三分之二的课题已经不属前沿，在美国
很少有人研究。在高校，一些教师治学严谨、基础扎实，但科研成果不突... 阅读全帖 |
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p**********d
发帖数: 7918 | 37 http://www.nytimes.com/2011/01/20/world/asia/20assess.html?hp
January 19, 2011
Subtle Signs of Progress in U.S.-China Relations
By MICHAEL WINES
WASHINGTON — The Chinese have striven to lend this week’s state visit by
President Hu Jintao the aura of a fresh start, from feel-good displays of
friendly Chinese in Times Square to a Washington newspaper insert that
declared on Wednesday that his meeting with President Obama could open a new
chapter in a relationship between the world’s two economic g... 阅读全帖 |
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J********y
发帖数: 263 | 38 That's something he said 20 years ago. There is no need to fixate on it. |
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s*********8
发帖数: 901 | 39 BEIJING – This weekend's arrival of a U.S. aircraft carrier in the Yellow
Sea poses a dilemma for Beijing: Should it protest angrily and aggravate
ties with Washington, or quietly accept the presence of a key symbol of
American military pre-eminence off Chinese shores?
The USS George Washington, accompanied by escort ships, is to take part in
military drills with South Korea following North Korea's shelling of a South
Korean island Tuesday that was one of the most serious confrontations since
th... 阅读全帖 |
|
s*********8
发帖数: 901 | 40 BEIJING – This weekend's arrival of a U.S. aircraft carrier in the Yellow
Sea poses a dilemma for Beijing: Should it protest angrily and aggravate
ties with Washington, or quietly accept the presence of a key symbol of
American military pre-eminence off Chinese shores?
The USS George Washington, accompanied by escort ships, is to take part in
military drills with South Korea following North Korea's shelling of a South
Korean island Tuesday that was one of the most serious confrontations since
th... 阅读全帖 |
|
x**n
发帖数: 1936 | 41 US carrier visit a dilemma for China
BEIJING – This weekend's arrival of a U.S. aircraft carrier in the Yellow
Sea poses a dilemma for Beijing: Should it protest angrily and aggravate
ties with Washington, or quietly accept the presence of a key symbol of
American military pre-eminence off Chinese shores?
The USS George Washington, accompanied by escort ships, is to take part in
military drills with South Korea following North Korea's shelling of a South
Korean island Tuesday that was one of the... 阅读全帖 |
|
t*******h
发帖数: 2882 | 42 大意：
世界粮农组织警告，中国干旱将迫使世界最大小麦生产者（中国）大量进口谷物，使得
市场价格创纪录的攀升。
埃及起义的根本原因，是中国：亚洲的繁荣造成了对谷物的空前需求。
40%的埃及人每天生活费低于2刀--甚至比加沙地带的人害穷。
食品的上涨加重了埃及人对现政权的不满。
埃及不能像中国在1949年一样搞土改，一切已经太晚了。
http://www.atimes.com/atimes/Middle_East/MB10Ak02.html
Chinese weather on Tahrir Square
By Spengler
The late novelist James Clavell had a tiresome habit of driving the plots of
his potboilers into cul-de-sacs, whence he would extricate them with a
natural disaster.
Egypt had its Clavell moment on February 8 when the Food and Agricul... 阅读全帖 |
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x****u
发帖数: 12955 | 44
路要
It's two symptoms of the same problem. The railroad department was so
fixated on obtaining certain numbers that they threw planning out of the
window, and forgot that the purpose of importing technology was never to be
dependent on that technology, but to spur domestic development. |
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s*********8
发帖数: 901 | 45 The anti-Wall Street protests, which are being driven from their urban
encampments across the nation, now face a pivotal challenge: With their
outposts gone, will their movement wither?
In New York, where the police temporarily evicted Occupy Wall Street
protesters from Zuccotti Park early Tuesday, and in other cities, dozens of
organizers maintained that the movement had already reshaped the public
debate. They said it no longer needed to rely solely on seizing parks,
demonstrating in front of ... 阅读全帖 |
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a********k
发帖数: 32 | 46 【紧急投票动员】改写亚裔孩子要比非裔SAT高450分才能入名校的现实
决战最高法院， 请投庄严一票，来捍卫你孩子公平竞争入学名校的机会
请置顶，请在以下网页签名，请转发！
http://admin.80-20nj.info/cgi/80/e?l=8/11e/f&w=no
-----------------
历史性的时刻已经到来了：2/21/2012，美国最高法院决定审理用“族裔”的理由来提
高亚裔和白人高校入学门槛的做法是否违宪。您--关爱孩子未来的父母--现在就可以花
一分钟时间来帮助孩子增大孩子上他们理想大学的机会。请您立即投票赞成（FOR） 80
-20全美亚裔教育基金会的民调书，清楚明白地表明亚裔在这件案子上的立场。
资料显示，亚裔在美国要比其他族裔成绩优秀许多才能上同样的学校。要上同样的名校
，亚裔SAT要考1550分，白人1410分，而非裔只需要1100分*（英文和数学满分是1600）
。亚裔入学这么高的门槛使得许多亚裔的大学申请者非常害怕，有些干脆拒绝列出他们
是亚裔—其实学校一看名字还是能知道哪些孩子是亚裔。。。
下面几周内，我们需要征集到至少五万个签名，让结果写入Am... 阅读全帖 |
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b****A
发帖数: 917 | 47 【 以下文字转载自 Immigration 讨论区 】
发信人: summercat (夏天的懒猫), 信区: Immigration
标 题: 【紧急投票】改写亚裔孩子要比非裔SAT高450分才能入名校的现实 (转载)
发信站: BBS 未名空间站 (Tue Feb 28 14:02:34 2012, 美东)
发信人: summercat (夏天的懒猫), 信区: NextGeneration
标 题: 【紧急投票】改写亚裔孩子要比非裔SAT高450分才能入名校的现实
发信站: BBS 未名空间站 (Tue Feb 28 14:01:24 2012, 美东)
请您立即投票赞成（FOR） 80-20全美亚裔教育基金会的民调书
请置顶，请在以下网页签名，请转发！
http://admin.80-20nj.info/cgi/80/e?l=8/11e/f&w=no
发信人: alderbrook (Alderbrook), 信区: Parenting
标 题: 【紧急投票】改写亚裔孩子要比非裔SAT高450分才能入名校的现实
发信站: BBS 未名空间站 (Tue Feb 28 01:... 阅读全帖 |
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t***s
发帖数: 300 | 48 【 以下文字转载自 Parenting 讨论区 】
发信人: alderbrook (Alderbrook), 信区: Parenting
标 题: 【请置顶 紧急投票】改写亚裔孩子要比非裔SAT高450分才能入名校的现实
发信站: BBS 未名空间站 (Tue Feb 28 01:38:53 2012, 美东)
【紧急投票动员】改写亚裔孩子要比非裔SAT高450分才能入名校的现实
决战最高法院， 请投庄严一票，来捍卫你孩子公平竞争入学名校的机会
请置顶，请在以下网页签名，请转发！
http://admin.80-20nj.info/cgi/80/e?l=8/11e/f&w=no
-----------------
历史性的时刻已经到来了：2/21/2012，美国最高法院决定审理用“族裔”的理由来提
高亚裔和白人高校入学门槛的做法是否违宪。您--关爱孩子未来的父母--现在就可以花
一分钟时间来帮助孩子增大孩子上他们理想大学的机会。请您立即投票赞成（FOR） 80
-20全美亚裔教育基金会的民调书，清楚明白地表明亚裔在这件案子上的立场。
资料显示，亚裔在美国要比其他族裔成绩优秀许多才能... 阅读全帖 |
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r*c
发帖数: 85 | 49 今天mailing list收到的。两个月的deadline，已经过去10天了，怎么都没看到人讨论
这个。写得这么好的文案，不广为传播真是浪费了
小声说一句，潜水员求置顶。。。
（以下为转载）
历史性的时刻已经到来了：2/21/2012，美国最高法院决定审理用“族裔”的理由来提
高亚裔和白人高校入学门槛的做法是否违宪。您--关爱孩子未来的父母--现在就可以花
一分钟时间来帮助孩子增大孩子上他们理想大学的机会。请您立即投票赞成（FOR） 80
-20全美亚裔教育基金会的民调书，清楚明白地表明亚裔在这件案子上的立场。
http://admin.80-20nj.info/cgi/80/e?l=8/11e/f&w=no
资料显示，亚裔在美国要比其他族裔成绩优秀许多才能上同样的学校。要上同样的名校
，亚裔SAT要考1550分，白人1410分，而非裔只需要1100分*（英文和数学满分是1600）
。亚裔入学这么高的门槛使得许多亚裔的大学申请者非常害怕，有些干脆拒绝列出他们
是亚裔—其实学校一看名字还是能知道哪些孩子是亚裔。。。
下面几周内，我们需要征集到至少五万个签名，让结果写入Amicus Cu... 阅读全帖 |
|
a***8
发帖数: 2433 | 50 http://admin.80-20nj.info/cgi/80/e?l=8/11e/f&w=no
紧急动员：决战最高法院， 请投庄严一票
捍卫你孩子公平竞争入学名校的机会
历史性的时刻已经到来了：2/21/2012， 美国最高法院决定审理用"族裔" 的理由来提
高亚裔和白人高校入学门槛的做法是否违宪。您--关爱
孩子未来的父母--现在就可以花一分钟时间来帮助孩子增大孩子上 他们理想大学的机
会。请您立即投票赞成（FOR） 80-20全
美亚裔教育基金会的民调书， 清楚明白地表明亚裔在这件案子上的立场。
http://admin.80-20nj.info/cgi/ 80/e?l=8/11e/f&w=no
资料显示， 亚裔在美国要比其他族裔成绩优秀许多才能上同样的学校。 要上同样的名
校，亚裔SAT要考1550分，白人1410分，
而非裔只需要1100分*（英文和数学满分是1600）。 亚裔入学这么高的门槛使得许多亚
裔的大学申请者非常害怕，
有些干脆拒绝列出他们是亚裔--其实学校一看名字还是能知道哪些孩 子是亚裔。。。
下面几周内，我们需要征集到至少五万个签名，让结果写入Amic us... 阅读全帖 |
|
