p****u
发帖数: 239 | 1 http://www.sciencedirect.com/science/article/pii/S1550413113003
Exercise can improve cognitive function and has been linked to the increased
expression of brain-derived neurotrophic factor (BDNF). However, the
underlying molecular mechanisms driving the elevation of this neurotrophin
remain unknown. Here we show that FNDC5, a previously identified muscle
protein that is induced in exercise and is cleaved and secreted as irisin,
is also elevated by endurance exercise in the hippocampus of mice. N... 阅读全帖 |
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I*******g
发帖数: 14 | 2 有谁知道内幕的展开说说。Irisin, FNDC5 story 好像不能hold up. 好多公司都不能
重复他的工作。是不是Spiegelman's work 都不大能让人相信。 |
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b****r
发帖数: 38 | 3 我承认我比较无聊。我不这个领域的，但我去查了一下。
abcam有两个抗体，
Anti-FNDC5 antibody - Carboxyterminal end
Anti-FNDC5 antibody (ab131390)
第一个抗体识别不了irisin，但第二个是可以。会不会他们用的是第二个抗体？
methods里也没提用的是哪一抗体。当然如果你有内幕消息的话就另当别论了。 |
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t*d
发帖数: 1290 | 4 真是有钱人会更有钱
Exercise benefits a variety of organ systems in mammals, and some of the
best-recognized effects of exercise on muscle are mediated by the
transcriptional co-activator PPAR-γ co-activator-1 α (PGC1-α). Here we
show in mouse that PGC1-α expression in muscle stimulates an increase in
expression of FNDC5, a membrane protein that is cleaved and secreted as a
newly identified hormone, irisin. Irisin acts on white adipose cells in
culture and in vivo to stimulate UCP1 expression and a broad ... 阅读全帖 |
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s*******e
发帖数: 1389 | 5 Fig.3的data全是另加的FNDC5吧？为什么全长的而不是irisin就可以browning呢？
in |
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b*******0
发帖数: 125 | 6 猜测这个实验应该是在Irisin鉴定出之前做的。直接用的是FNDC5.
irisin也有browning的效果，见fig6 |
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b*******0
发帖数: 125 | 7 1.Pekkala, S., P. Wiklund, J. J. Hulmi, J. P. Ahtiainen, M. Horttanainen, E.
Pollanen, . . . S. Cheng. 2013. Are Skeletal Muscle FNDC5 Gene Expression
and Irisin Release Regulated by Exercise and Related to Health? J Physiol.
in press.
2.Raschke S, Elsen M, Gassenhuber H, Sommerfeld M, Schwahn U, et al. (2013)
Evidence against a Beneficial Effect of Irisin in Humans. PLoS ONE 8(9):
e73680. doi:10.1371/journal.pone.0073680 |
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A******y
发帖数: 2041 | 8 Yes, Bruce M. Spiegelman's post-docs, when irisin worked in human then let
us know. Sounds like another leptin... |
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