y*****w
发帖数: 146 | 1 实验课题需要用到Yeast GAL1和GAL10 promoters.在addgene 上查到了GAL1 promoter
sequence， 但是怎么google都查不到GAL10 promoter sequence。有谁知道Yeast
GAL10 promoter sequence? 请发邮件至y******[email protected] 十分谢谢! |
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y*****w
发帖数: 146 | 2 再问一下，有谁知道GAL4 promoter 的sequence吗？ GAL1， GAL10， and GAL4 这3个
promoters 哪个的strength最强呢？十分感谢！ |
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h**********r
发帖数: 671 | 3 必须是pESC系列。
当时我还问大家要不要加kozak序列，也没说出个所以然来。后来我终于等到了客服的
答复。下面就是：
The GAL1 and GAL10 yeast promoters from the pESC-Leu vector have the FLAG or
the MYC epitopes in the two MCS of the vector. Both include ATG codons at
the 5’ end of the FLAG and the MYC epitopes. If you clone into the Bgl II
or the Xho I site in frame with the ATG of the epitope tag, you will have
the start codon, kozak sequence and should get good expression of your N-
tagged protein. If you need the epitope tag at the C-terminal, ... 阅读全帖 |
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z*******n
发帖数: 101 | 4 Check pesc vector set manual. |
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e***o
发帖数: 344 | 5 搭车问一个yeast two hybrid的HIS 基因和它的启动子gal4的序列。就是整合在yeast基因组
里，gal4 promoter drive的一个筛选标记基因。
谢谢！ |
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