b*********8 发帖数: 44 | 1 最近想做酵母双杂交,但发现现在的载体(pGADT7/pGBKT7, pDEST22/pDEST32)都是把
目标蛋白融合到Gal-AD或Gal-BD的C端。 我的蛋白融合到C端不表达,请问有Gal-AD或
Gal-BD在C端,这样目标蛋白可以融合到Gal-AD或Gal-BD的N端的Y2H载体吗?非常感谢
!有Gal-AD或Gal-BD在C端的载体吗? |
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x*******i 发帖数: 1590 | 2 1,full amount就是一次用完2000点,每gallon省2快,你只加1gallon就省2
快,加10gallon省20快,在 35 gal内都可以省2快/gal.
2,choose amount,然后就跳出:use 10 to 200 cents,选200,就是2快,即2000点
,省2快/gal,当然点数全用完.你可以选10 to 200,比如选20 cents,即省2毛/
gal,就只扣200点,留1800点下次用。
3,up to 35 gal是指一次加油,在 35 gal内都可以省2快/gal,如果你有2000点.
4,点数浪费的问题,我都是一次用完,use full amount.除非你能预计油价涨跌。
use |
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k***y 发帖数: 217 | 3 it's time we turned our attention to a female
equivalent - girl, gal, and gel. I hope you don't need me to tell you how
the word girl is used, but here are a few Bank of English examples for
gal and gel:
...Roseanne Barr, a real funny gal.
Johnny would ask her, `You all right, gal?'
The bells are ringing for me and my gal.
Glenn Close apart, most American stars avoid bad-guy or bad-gal roles.
We're in trouble, here guys and gals.
...solid soldiers, regul |
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a*********0 发帖数: 4998 | 4 “$0.03每加仑,虽然算起来连1%都不到”
这是啥状况? $0.03/gal挺好的呀
它家主页有排行榜,今日某州某城某人0.01/gal,列个光荣榜,
我不想登榜,就弄到0.1x/gal去加了两次,挺神奇的,光看gal的数字猛涨,
price那边慢慢的最后升到$1.xx
两桶油加的甚爽,还特地拎个油桶凑到20 gal回家给另一个车子也加满 |
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t******k 发帖数: 5617 | 5 transgenic mice的lacZ staining和senescence associated b-Gal staining (SA-b-
Gal)是不是染色的PH条件不同?
如果有lacZ的b-Gal staining kit能不能用来测SA-b-Gal? |
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c*****9 发帖数: 2739 | 6 二手交易风险自负!请自行验证是否合法和一手卡!:
我想卖的物品:
2200 StopShop Gas Points for 35 Gal. Shell
单张面值:
2200 StopShop Gas Points for 35 Ga Shell
可接受价格(必须明码标价!):
2200 StopShop Gas Points for 35 Gal. Shell @20, expired tonight
物品新旧要求:
2200 StopShop Gas Points for 35 Gal. Shell @20
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
Paypal
其他补充说明:
广告的有效期:
物品来源(Required for All Cards!):
我的联系方式:
Mitbbs
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
state and zip:
CT |
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s*********i 发帖数: 13 | 7 K,我这里油价$3.59/gal,租的车加不起,只能几gal的一点点加,目前看到最便宜的
是$3.27/gal。抢钱阿 |
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y****e 发帖数: 23939 | 8 钱包里正好有最近的几张加油收据,10月份是3.37/gal,11月是3.07/gal,12月就是2.
95/gal,这走势看明年会不会跌到2块以内吧。 |
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w*******y 发帖数: 60932 | 9 Porter-Cable Pin Nailer + 6 Gal. 150 psi Compressor $121 shipped from Home
Depot
$169 - $70 (Promo Code) = $99 + $21.99 shipping = $120.99
Use Promo Code MAY99Durable, maintenance-free pump/motor - no oil spillage
Pankcake compressor weighs 34 lbs. - lightweight for greater portability
Pin nailer drives 23 gauge nails from 1/2 in. (12.7 mm) to 1 in. (25.4 mm)
length into solid oak
Kit includes : 6 gal. compressor, pin nailer, 25 ft. x 1/4 in. hose with
fittings attached, 3/4" 23 gauge pin nails,... 阅读全帖 |
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b****r 发帖数: 1272 | 11 上2个月用了23000 GAL
这个算正常嘛?
我算算一天怎么也用不了200 GAL啊
合60块钱一个月 |
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h*******n 发帖数: 980 | 12 佩服大油箱。
偶是22加仑油箱,外加两个5加仑油桶,每次在frys只能加30-32 gal @ 98 cent/gal。
不过safeway limit是25加仑,就毫无压力。
十年一梦啊,没想到还能像2000年左右一样加到低于1刀的油。 |
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s****e 发帖数: 3 | 13 【 以下文字转载自 Girl 讨论区 】
【 原文由 sallie 所发表 】
dunno any dialects... :( how sad..
but just curious.. is "gal" bostonian? say "this gal is wicked good"? |
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w*******y 发帖数: 60932 | 14 * Ultra Hot - Last Day * Arrowhead Spring Water 2.5 Gal. for .32 (must buy 6
for $1.94) at Smart and Final B&M (western states)
Arrowhead Spring Water 2.5 Gal. on sale for $1.99
When you buy 6, you get $10 off instantly from Nestle. (It's in their ad.)
6 x $1.99 = $11.94 - $10 = $1.94
Hurry, last day.
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a*****n 发帖数: 2499 | 17 夏威夷更好,$3/Gal。
不过呢,屁大个岛,不小心能开到海里 |
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p***n 发帖数: 17190 | 19 就是公分每秒每秒
那重力加速度就是
980 gal了
剛剛查了一下地震單位 |
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l****z 发帖数: 29846 | 20 【 以下文字转载自 WaterWorld 讨论区 】
发信人: yushen1989 (Maple), 信区: WaterWorld
标 题: Re: $2.95/gal, 油价怎么这么低了啊
发信站: BBS 未名空间站 (Sat Dec 22 23:44:33 2012, 美东)
我这还是3.3我去 |
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w*****s 发帖数: 4116 | 21 二手交易风险自负!请自行验证是否合法和一手卡!:
Y
我想卖的物品:
Kroger Shoppers Card 4000points $1/gal on GAS
单张面值:
Save $1 per gallon if you fill gas at Kroger gas station
可接受价格(必须明码标价!):
$20 per card with 4000 points
Points will expire on 03/31/2011
物品新旧要求:
two shoppers(membership) card in hand
邮寄方式要求:
FREE FC mail, if lost, I refund you
买卖双方谁承担邮寄损失(Required if not code only):
me
付款方式说明:
non-cc paypal/BOA
其他补充说明:
You MUST make sure that you have Kroger with their gas station in your city/
town. Each card has 4000 points. ... 阅读全帖 |
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e*o 发帖数: 287 | 22 One for 10.
up to 20 gal. paypal.
REDEEMABLE THRU 12/7. Can combine with American Express card offer 5 off 25
now.
PM CODE.
To redeem code
1.go inside and press the loyalty ID key on the pin pad.
2. enter the 10 digit code.
3. select yes to redeem and follow prompts for payment
4. pump gas. |
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w*****s 发帖数: 4116 | 23 bought $500 gift card and get 2000 points for $2 off /gal gas. 4x promotion
ending 06/16. This is highly YMMV deal. I am around Richmond, VA. |
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d*******h 发帖数: 5065 | 24 这个不算啥,俺一般只加2 cents/gal的油 |
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e*o 发帖数: 287 | 25 One for 10.
up to 20 gal. paypal.
REDEEMABLE THRU 12/7. Can combine with American Express card offer 5 off 25
now.
PM CODE.
To redeem code
1.go inside and press the loyalty ID key on the pin pad.
2. enter the 10 digit code.
3. select yes to redeem and follow prompts for payment
4. pump gas. |
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a*x 发帖数: 131 | 26 When does the 3¢/gal FRN Member Reward end?
August 5, 2013
抓紧用吧 |
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f********e 发帖数: 1298 | 27 就算按最便宜的87号3.4/gal都不到5%...
6月有AMEX的exxon送钱offer, 下个月freedom和discover加油都是5%...
bb这个没啥吸引力啊 |
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a*********k 发帖数: 29 | 28 不建议交易打印胖子;胖子是免费的,收费的是服务:
求购眼药水
所求物品名称:
BLINK TEARS /REFRESH(不要GAL TEARS)
物品类别(coupon: mfc 等;血糖仪等):
物品来源(报纸夹页,厂家邮寄等):
各大药店
可接受的价格(必须明码标价,必填):
1USD/瓶 10瓶以上 去年买过的。自用,因为手术长期需要。谢谢
邮寄损失方式哪方承担(若需邮寄,必填):
买方承担
付款方式说明:
PAYPAL
本贴有效期(必填):
联系方式(例: 站内):
我的邮编是64086。y********[email protected]. OR站内 |
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s*******8 发帖数: 444 | 29 现在50off,Gal装的89分。我家一直当饮用水喝。用上官网上55分off2的coupon,算下
来才6毛多1桶。
cvs还有些香皂,沐浴露都在打折,估计要换新包装,把旧的清仓。 |
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h*********w 发帖数: 171 | 30 买500gc,得2千点,可是加油的时候,有3个选择,我选1:use full amount,那我的
车只加10gal不到,可是点数全部没了。后来我选2:choose amount,然后就跳出:use
10 to 200 cents,我选200,于是点数还是全用完了。
那这个up to 35 gal,到底要怎么用,可以多次使用,不浪费点数?谢谢 |
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x*******i 发帖数: 1590 | 31 2辆车倒不难,2个人开2辆车去,一个人先给第一辆车加油,加完后,拿着油枪,别
放回去,第二个人上来把第一辆车开到一边停好,过来把第二辆车开到油泵前加油,以
此类推,多少辆都型,up to 35 gal. |
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x*******i 发帖数: 1590 | 32 only uo to 35 gal limit,
no how many cars limit.
one car is like one 油壶.
so 油壶也限制? |
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j*****g 发帖数: 7513 | 33 一般家里的垃圾桶30Gal(一周倒一次), 要买40 gal 垃圾袋。
垃圾桶有不同size, 买相应的垃圾袋 |
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w****r 发帖数: 15252 | 34 【 以下文字转载自 Money 讨论区 】
发信人: Diors (屌丝), 信区: Money
标 题: Save 15c/gal of gas with Bluebird by Sep. 8
发信站: BBS 未名空间站 (Wed Jun 4 09:15:54 2014, 美东)
收到的email
Save up to 15c per gallon
At participating Walmart or Murphy USA gas stations with the Walmart Credit
Card, Walmart MoneyCard or Bluebird by American Express Card from 5/18/2014
to 09/08/2014.
No Walmart Credit Card, Walmart MoneyCard or Bluebird by American Express
Card? No Problem! Use your Walmart gift card today to save 10per gallon*
throug... 阅读全帖 |
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w**********9 发帖数: 1098 | 36 哪里有便宜的油Cap?我Cap的公司涨到3.99/Gal了 |
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a****e 发帖数: 13 | 37 搬家出售5加仑小水族箱(鱼缸)包括两条金鱼和所有配件--$45
--Fish tank (5 gal)- paid for $35
--Heater included --paid for $15
--New Filter included --paid for $15
--Two fish- paid for $10
---Marbles-paid for $10
--Plant paid for $8
-Fish food paid for $8
Location: Smyrna, GA (Cobb, Marietta, Vinings)
请点击看照片:
https://picasaweb.google.com/104867894252394437204/MovingSale
请联系:
l*********[email protected]
Thanks, |
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w****8 发帖数: 81 | 38 A new 132 gal new deck box for sale, I bought it for $120 to store my stuffs
in the underground parking garage, but the property manager said it was too
big and didn't let me to place it, so I have to sell it for $60.
(location: Chesterfield, MO). |
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a******o 发帖数: 16625 | 42 加州汽油税已经是全国最高的了,>65c/gal |
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f***r 发帖数: 2916 | 43 从walmart看了有primo的水,拿着回收折扣也要6.5刀,比直接买5瓶1 Gal的都贵
再看其他的,要么只有送货上门,要么是refill
不想买送货上门的,只想自己需要时候去买
也不想refill,觉得瓶子重复使用不好 |
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P*A 发帖数: 7996 | 44 是啊,俺从来不信任重复使用的瓶子,也不信任这种开了口放好多天的水
还是买瓶装水合适,24瓶比3 Gal多些,品牌货也就4块钱,house brand基本都不到3块 |
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v***w 发帖数: 339 | 45 【 以下文字转载自 FleaMarket 讨论区 】
发信人: viviw (vivi), 信区: FleaMarket
标 题: 【出售】+++Giant gas rewards saving $2.2/gal+++
发信站: BBS 未名空间站 (Sat May 3 21:17:03 2014, 美东)
二手交易风险自负!请自行验证是否合法和一手卡!:
我想卖的物品:
Giant gas rewards
单张面值:
2200 points
可接受价格(必须明码标价!):
$16
每次可以省$2.2/Gallon, max 35 gallons each fill-up
物品新旧要求:
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
其他补充说明:
广告的有效期:
20 days
物品来源(Required for All Cards!):
Giant food store
我的联系方式:
站内
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
state and ... 阅读全帖 |
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t****7 发帖数: 11132 | 48 真浪费啊,偶都是直接加 35gal@ 99cent/gal. |
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h*******n 发帖数: 980 | 49 我们这里safeway up to $2 off, but limit to 25 gal |
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y****e 发帖数: 23939 | 50 TX,昨天加的。今天路过一个加油站$2.87/gal,亏了 |
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