w*********e
发帖数: 98 | 1 6%-7% running gel. You still need a stacking gel (2%-4%)
? |
|
s******y
发帖数: 28562 | 2 我倒。。。
你是说配gradient gel?
还是一般的8%, 10% 的胶？
precast gel 的意思就是公司事先配好的。。。 |
|
s********n
发帖数: 2939 | 3 对啊，E-Gel也有protein gel的。
OK，没想到是不同抗体，实在不行就转膜后再切下来，不过得到的膜会比较小，不知道
做western是否方便了。 |
|
s******a
发帖数: 472 | 4 Is it possible? What gel concentration should I use?
I want to do a gel extraction.
Thanks. |
|
a****k
发帖数: 1130 | 5 没做个这么小的蛋白，在那个60kd的峰里应该主要还是你的蛋白吧？如果是的话，我猜
你的蛋白很可能是以oligomer存在的（当然，根据我们的课本知识，gel filtration不
光是size有影响，还有structure。。。）注意到你buffer的盐浓度算是有点高的了，
在这样的浓度下还能结合的，应该属于interaction比较强了吧。不知道具体是个什么
蛋白，但是根据自己的经验，如果是这样的话，monomer很可能是没有activity的，必
须要以这样的形式存在才行。你在60kd看到的其他杂带有可能是因为他们本身就是那个
size附近的，或者试试ion exchange来去除杂蛋白？如果一定要用gel filtration，可
以在前面加一步ammonium sulfate precipitation试试，有可能把你的蛋白和杂蛋白分
开一些，但是这个就要摸条件了
NaCl, |
|
G***G
发帖数: 16778 | 6 问个问题。
2d gel做了两个图片，一个是cancer，一个是normal
现在用pdquest找出了图片中不同的spots。
问题是对于某一个spot，它在cancer中，和在normal中对应的蛋白质是一样的吗？
也就是2d gel的图片中（两个图片，一个是cancer的，一个是normal的）相同位置的点
，就一定对应相同的蛋白质吗？ |
|
K******S
发帖数: 10109 | 7 2D gel is dying in US. Lots of ppl in China and Europe still use it. And PMF
is dying as well. Get MS/MS if you can. 误差 is not major issue for PMF.
PMF can't handle complex samples. With current MS technology, you can ID
multiple proteins even from 2D gel spots. |
|
f********y
发帖数: 394 | 8 需要在pH6.0条件下跑gel shift， 普通的TBE buffer的pH不合适。
有谁知道用什么buffer在酸性下适合跑gel shift？
跪谢 |
|
t**l
发帖数: 109 | 9 现在实验室还是用那种小刀片，每次都要把手伸到UV light里面才可以，有没有那种可
以有把手的刀，可以远距离操作，或者可以把一次性的刀片组装上面的那种工具。多谢
。因为预计要做大量的gel extraction. 不想伤到自己。大家切gel的时候都怎么做的 |
|
s******s
发帖数: 13035 | 10 我说了有20遍了，qiagen gel extraction以后，通通过一遍minElute浓缩换buffer
Gel |
|
m**l
发帖数: 15 | 11 I am reading on hyperbranched materials and lots of people mention gel points,
but I can't find any detailed discription of what it is and what it looks
like. For example, is it a function of monomer concentration or something
else? Is it literally a gel or just a insoluble solid?
Thanks! |
|
r******0
发帖数: 2753 | 12 I think the author was right. Gel could be solid and fluid like; and G">G' is
one
of the widely used criteria for gel point. Wond, if you have any doubt, you
may double check it with Wang, SQ. I belive it was him told me that. hehe...
them |
|
w********h
发帖数: 12367 | 13 个人感觉这个条件既不充分，也不必要。
倒象是block copolymer disorder to order transition在terminal regime的转折
点。
我们看一个东东是不是gel，首先要看这种weak association是不是足够强壮到出现
G'>G"的plateau regime. 如果作者根本就没有有强有力的证据说明这个gelation，
比如(1) 粘度是不是随温度etc有个sharp的增加？(2) 在某些 frequency region
G'>G". 如果这两个证据都没给，反而告诉我这个gel是fluid-like，我不能接受。
G">G'
】
is
differiente |
|
c*****e
发帖数: 238 | 14 I doubt it, both moduli depend on the frequency, and in the gel phase
the dependences are both nontrivial and different: one goes to constant at
large frequency and vanishes at small frequency, the other gradually decreases
at large frequency but remains finite at small frequency.
How can their ratio become independent of frequency?
I am referring to physical gels. |
|
C*******b
发帖数: 174 | 15 两种高分子链，一个分子链的端基和另一个高分子链上的基团相互反应，理论上能形
成gel。但是如何计算能形成gel所需要的最低浓度？谢谢了。 |
|
n******d
发帖数: 3583 | 16 REI每个月都有一种gel特价，一般一小包大概$0.89
而且现在骑车，我喜欢吃sports bean，不像吃gel那么一不小心就粘得一塌糊涂。
http://sportbeans.com/ |
|
|
w*******y
发帖数: 60932 | 18 OfficeMax.com 100% back in MaxPerks rewards on gel pens, highlighters and
correction film 01/16/2011 - 01/22/2011
Online Only.
"It's like getting FREE writing instruments after 100% back in MaxPerks
Bonus Rewards on select items." link:
http://www.officemax.com/promotional-pages/maxperks-member-spec
Paper Mate Retractable Gel Pens, Medium 12-Pack, available in black and blue
(item number 21728347, 21728365) $12.99 limit 3 www.officemaxperks.com:
Sharpie Accent Tank-Style Highlighters, Chisel 1... 阅读全帖 |
|
w*******y
发帖数: 60932 | 19 CrayolaColor Explosion Glow Board with Gel Clings
# Make amazing drawings with 3-D effects
# Drawing surface is reusable, making for hours of fun
# Special lights inside Glow Board work with the marker ink for bright fun
# Two Glowing gel clings add special effects and dimension
# Recommended for ages 6 and up
Link:
http://www.staples.com/Crayola-Color-Explosion-Glow-Board-with-
|
|
w*******y
发帖数: 60932 | 20 Insertable Binder 5-Tab Dividers .89, uni-ball Retractable Gel Pens, 2 Blue
$1.32, Vivo Mechanical Pencils, 12-Pack $1.19, Mead Spiral Notebook .56,
Washable Crayons, 8 Colors .66 free shipping with Prime (FSSS) from Amazon
Sorry wasn't able to fit the store in the title
Vivo Grip Mechanical Pencils, Refillable, 0.7 mm, 12-pack, $1.19
Wilson Jones Insertable Binder 5-Tab Dividers, Multicolor .89
Mead Spiral Notebook, 1-Subject, 70-Count, Wide Ruled, Yellow .56
Dixon Prang Washable Crayons, 8 Col... 阅读全帖 |
|
w*******y
发帖数: 60932 | 21 Seemed pretty good to me.
I use the energy gels for workouts. .58 per gel is the best deal I've ever
found on them.
Here's the link:
Link:
http://www.gnc.com/family/index.jsp?categoryId=3703232&f=PAD/Br
PowerBarProtein Plus - Chocolate Peanut Butter - @50% off: 13.445 (1.12
each)
PowerBarPerformance Energy Bar - Chocolate- @50% off: 8.34 (0.695 each)
PowerBarPerformance Energy Bar - Cookies & Cream- @50% off: 7.74 (0.645
each)
PowerBarPerformance Energy Bar - Milk Chocolate Brownie- @50% off: 7... 阅读全帖 |
|
w*******y
发帖数: 60932 | 22 1. Login or create a Medco Account:
http://www.medcohealthstore.com
2. Next, find the product & change the quantity to 3 Medco Account:
http://www.medcohealthstore.com
3. View your cart. You should see the 3 Spray Gels for $0 + $.99 shipping
*Note* If you add the Spray Gel from the product page itself, it is not
giving you the discount. You need to add the items to your cart from the
link I provided.
|
|
w*******y
发帖数: 60932 | 23 Garnier Fructis Style Brilliantine Shine Cream Gel Extra Strong 6.8 oz for
95 cents with free shipping using Amazon Prime.
Sold by third-party, fulfilled by Amazon here:
http://www.amazon.com/gp/product/B0013L5GU4
I've never used it, but I'm not picky about hair gel so I will give it a try.
|
|
|
w*******y
发帖数: 60932 | 25 Hello all. This is my first time trying to post a deal, so please let me
know if I'm doing this wrong.
The Body Shop is currently having their semi-annual sale. Most of the deals
are decent but not amazing (body butters down to $10 from $16), but I saw
one deal that looked quite good to me. The passion fruit cleansing gel is
down to $2 from $5.99, and it's buy one get one free.
This is the gel:
http://www.thebodyshop-usa.com/skin-care/face-wash/passion-frui
. It's possible this is also available... 阅读全帖 |
|
w*******y
发帖数: 60932 | 26 Did a search for "Target Edge, Edge shaving, Edge cream" but didn't find
anything...but took this fromLink:
http://hoknowsdeals.com/
.
My Local Target has the Edge Shaving Gel or Skintimate Shaving Cream on
clearance for $0.52. Will print out the coupon and come back later. This
is YMMV.
Use this$0.75 off one Edge or Skintimate shave gel Coupon from Coupons.com:
http://www.coupons.com/alink.asp?go=13903xh2010&bid=鉂121742000
$0.52 - $0.75 = Free!
I don't think Target pays you out for overages ... 阅读全帖 |
|
w*******y
发帖数: 60932 | 27 Enjoy the invigorating clean of this 2-in-1 shower gel and hair wash for men
from White Rain It features a cool ocean wave scent and is enriched with
vitamin E, aloe and sea salt.
White Rain2-in-1 shower gel and hair wash for men
Cool ocean wave scent
Enriched with vitamin E, aloe and sea salt
18 fl oz
Link:
http://www.dollargeneral.com/product/index.jsp?productId=118960
USE Promo Code: DG10FIRST
|
|
w*******y
发帖数: 60932 | 28 The Body Shop:
http://www.thebodyshop-usa.com/index.aspx?cm_mmc=LS-_-Default-_
is currently running many New Year's promotions, but the best one IMO is
the 4 shower gels(8.4oz) for $16. I really like their shower gels and i
recommend the strawberry and almond scented ones
Shipping is free with code: FREEJOY
|
|
|
w*******y
发帖数: 60932 | 30 Cigar Caddy Crystal Humidifier Gel 2oz. $4.50 Shipped
Negotiated the gel down to $2.00 plus 2.50 shipping.
Link:
https://www.cigarbargains.com/negotiations/IDQGQPUH
Suzy:
Our current price is $9.99. Enter your offer below and click MAKE OFFER.
Josh:
I'll offer you $1.50
Suzy:
That price is too low, Josh. How about $8.61?
Josh:
I'll offer you $1.51
Suzy:
That price is too low, Josh. How about $6.68?
Josh:
I'll offer you $1.53
Suzy:
That price is too low, Josh. How about $4.89?
Josh:
I'll offer y... 阅读全帖 |
|
a******X
发帖数: 122 | 31 我想要的物品:
黄油Gel+礼包@Dillards dillards 60套
不需要分箱～量大优惠～
my lable
单张面值:
24+tax, minus potential gc discount
可接受的价格(必须明码标价！):
$28+ML
物品新旧要求:
邮寄方式要求:
my lable
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
BOA/PAYPAL/BILL PAY/RMB
其他补充说明:
广告的有效期:
物品来源:
dillards
我的联系方式:
PM
二手交易风险自负！请自行验证是否合法和一手卡！ |
|
l*****m
发帖数: 250 | 32 不建议交易打印胖子；胖子是免费的，收费的是服务:
胖子免费
出售/交换物品的名称:
免费BEGAY PAIN RELIEF + MASSAGE GEL REBATE
物品类别（coupon：mfc等；血糖仪等）:
REBATE，06/20-08/20/10之间购买，UP TP $8.99
物品来源（报纸夹页，厂家邮寄等）:
报纸剪下来的，网上买多的。
可接受的价格（必须明码标价，必填）:
免费
邮寄损失方式哪方承担（若需邮寄，必填）:
RECEIVER
付款方式说明:
PAYPAL
本贴有效期（必填）:
送完为止
联系方式（例： 站内）:
站内 |
|
j*****y
发帖数: 25 | 33 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
Clinique Moisturizing Gel/黄油 125ml 南加
单张面值:
$24+tax
可接受价格(必须明码标价！):
0.80
物品新旧要求:
new
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
BOA
其他补充说明:
广告的有效期:
物品来源(Required for All Cards!):
Macy\'s
我的联系方式:
Tel:626-283-8980
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
state and zip: |
|
a*********r
发帖数: 346 | 34 Lancome 小黑瓶眼部和面部各1个
La Mer gel creme and hydra infusion
希望价格白菜点 |
|
c********y
发帖数: 636 | 35 不建议交易打印胖子；胖子是免费的，收费的是服务:
所求物品名称:
bayer 胖子3张
schick hydro shave gel 1off 胖子 4张
物品类别（coupon: mfc 等；血糖仪等）:
Coupon
物品来源（报纸夹页，厂家邮寄等）:
报纸
可接受的价格（必须明码标价，必填）:
0.1each.
邮寄损失方式哪方承担（若需邮寄，必填）:
付款方式说明:
paypal
本贴有效期（必填）:
联系方式（例： 站内）:
站内 |
|
p*****1
发帖数: 128 | 36 我想卖的物品:
黄油gel 125ml
可接受价格(必须明码标价！):
210元/瓶
物品新旧要求:
全新
邮寄方式要求:
承担中国国内邮费
付款方式说明:
支付宝
广告的有效期:
TILL GONE
物品来源(Required for All Cards!):
Outlet
我的联系方式:
站短 |
|
i*o
发帖数: 202 | 37 1 我想卖的物品: Bobbie Brown Long-Wear Gel Eyeliner 色号是 Graphite
Shimmer
2 付款方式：最好有在波士顿的jm 当面现金交易 因为只有一个 不好寄
5 其它补充说明 超级新手 价格如果不合理请不吝赐教
6 我的联系方式 站内信件，please |
|
l********7
发帖数: 530 | 38 不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
$10off Neuragen PN .17 oz or Neuragen Gel 0.28oz
物品类别（coupon：mfc等；血糖仪等）:
mfc
物品来源（报纸夹页，厂家邮寄等）:
报纸夹页
可接受的价格（必须明码标价，必填）:
换 -$5 Neuragen PN .17 oz bottle (off Regular Price) exp 123110 page 20
diabetes & you magazine fall 2010
邮寄损失方式哪方承担（若需邮寄，必填）:
付款方式说明:
本贴有效期（必填）:
联系方式（例： 站内）: |
|
S********a
发帖数: 56 | 39 二手交易风险自负！请自行验证是否合法和一手卡！:
y
我想卖的物品:
LA现货 倩碧黄油lotion gel 有bump 和无bump
单张面值:
24+tax
可接受价格(必须明码标价！):
22
物品新旧要求:
New in box 有小票
邮寄方式要求:
Your label.
买卖双方谁承担邮寄损失(Required if not code only):
before in the system, mine, after in the system, yours.
付款方式说明:
BOA or paypal.
其他补充说明:
广告的有效期:
til gone
物品来源(Required for All Cards!):
APPLE store
我的联系方式:
email l******[email protected]
or call 818 395 6922
Warranty期限:
都是最近现货，有小票
能否证明是合法的一手卡?(Required for All Cards!):
yes.
state and zip:
CA 91801 |
|
S********a
发帖数: 56 | 40 二手交易风险自负！请自行验证是否合法和一手卡！:
y
我想卖的物品:
LA现货 倩碧黄油lotion gel 有bump 和无bump
单张面值:
24+tax
可接受价格(必须明码标价！):
22
物品新旧要求:
New in box 有小票
邮寄方式要求:
Your label.
买卖双方谁承担邮寄损失(Required if not code only):
before in the system, mine, after in the system, yours.
付款方式说明:
BOA or paypal.
其他补充说明:
广告的有效期:
til gone
物品来源(Required for All Cards!):
APPLE store
我的联系方式:
email l******[email protected]
or call 818 395 6922
Warranty期限:
都是最近现货，有小票
能否证明是合法的一手卡?(Required for All Cards!):
yes.
state and zip:
CA 91801 |
|
l********t
发帖数: 115 | 41 我想卖的物品:
倩碧黄油lotion with pump 37个.
倩碧黄油gel with pump 11个.
Lord & Taylor 12月的礼包 7个.
单张面值:
24+tax
可接受价格(必须明码标价！):
黄油 $21.5/个,礼包 $10/个.
物品新旧要求:
All New in box
邮寄方式要求:
Your label.
买卖双方谁承担邮寄损失(Required if not code only):
before in the system, mine, after in the system, yours.
付款方式说明:
paypal or billpay or BOA.
其他补充说明:
广告的有效期:
til gone
物品来源(Required for All Cards!):
Lord & Taylor
我的联系方式:
BBS mail.
Warranty期限:
都是最近一个星期的现货，2010年批号.
能否证明是合法的一手卡?(Required for All Cards!):
yes.
state and zip:
19380 |
|
l********t
发帖数: 115 | 42 我想卖的物品:
倩碧黄油lotion with pump 37个.
倩碧黄油gel with pump 11个.
Lord & Taylor 12月的礼包 7个.
单张面值:
24+tax
可接受价格(必须明码标价！):
黄油 $21.5/个,礼包 $10/个.
物品新旧要求:
All New in box
邮寄方式要求:
Your label.
买卖双方谁承担邮寄损失(Required if not code only):
before in the system, mine, after in the system, yours.
付款方式说明:
paypal or billpay or BOA.
其他补充说明:
广告的有效期:
til gone
物品来源(Required for All Cards!):
Lord & Taylor
我的联系方式:
BBS mail.
Warranty期限:
都是最近一个星期的现货，2010年批号.
能否证明是合法的一手卡?(Required for All Cards!):
yes.
state and zip:
19380 |
|
l********t
发帖数: 115 | 43 我想卖的物品:
倩碧黄油lotion with pump 37个.
倩碧黄油gel with pump 11个.
Lord & Taylor 12月的礼包 7个.
单张面值:
24+tax
可接受价格(必须明码标价！):
黄油 $21.5/个,礼包 $12.50/个.
物品新旧要求:
All New in box
邮寄方式要求:
Your label.
买卖双方谁承担邮寄损失(Required if not code only):
before in the system, mine, after in the system, yours.
付款方式说明:
paypal or billpay or BOA.
其他补充说明:
广告的有效期:
til gone
物品来源(Required for All Cards!):
Lord & Taylor, macys, bloomingdale's
我的联系方式:
BBS mail.
Warranty期限:
都是最近一个星期的现货，2010年批号.
能否证明是合法的一手卡?(Required for All Cards!):
yes.
stat... 阅读全帖 |
|
t********o
发帖数: 185 | 44 我想卖的物品:
倩碧黄油lotion,gel with pump 38个, lotion 为主.
Kiehl's 牛油果眼霜 6个
单张面值:
24+tax
25.5+tax
可接受价格(必须明码标价！):
黄油 $21.5/个
牛油果眼霜 $ 23.5/个
物品新旧要求:
All New in box
邮寄方式要求:
Your label.
买卖双方谁承担邮寄损失(Required if not code only):
before in the system, mine, after in the system, yours.
付款方式说明:
paypal or BOA.
其他补充说明:
只要黄油也可以
广告的有效期:
til gone
物品来源(Required for All Cards!):
macys, bloomingdale's
我的联系方式:
mitbbs
Warranty期限:
都是2010年批号.
zip:
15217 |
|
s*****c
发帖数: 5202 | 45 不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
10off Neuragen pn 0.17floz or neuragen gel 0.28oz
物品类别（coupon：mfc等；血糖仪等）:
报纸coupon
物品来源（报纸夹页，厂家邮寄等）:
报纸夹页
可接受的价格（必须明码标价，必填）:
0.2each+0.5邮资
邮寄损失方式哪方承担（若需邮寄，必填）:
付款方式说明:
non-cc paypal
本贴有效期（必填）:
胖子1/31过期，可用rite aid 1/30开始的neuragen 19.99scr
联系方式（例： 站内）:
站内 |
|
l********t
发帖数: 115 | 46 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
黄油lotion gel 11个
单张面值:
$24.5
可接受价格(必须明码标价！):
$22
物品新旧要求:
10年批号。
邮寄方式要求:
YL.
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
billpay or BOA or non-cc paypal.
其他补充说明:
广告的有效期:
物品来源(Required for All Cards!):
我的联系方式:
BBS mail.
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
state and zip: |
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r*****n
发帖数: 566 | 47 paypal付款，站内联系
1. Shiseido White Lucent Brightening Moisturizing Gel
40ml /1.4 oz $40包邮
无盒子，但是盖子里面是sealed
批号： 3TPSV |
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n**********u
发帖数: 739 | 48 2010年批号，with pump
40个有油的，40个无油的GEL
BOA 转账
SHIP TO 美国境内。 |
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w******s
发帖数: 768 | 50 二手交易风险自负！请自行验证是否合法和一手卡！:
Y
我想卖的物品:
30套倩碧黄油(gel)+礼包
单张面值:
$24.5+Tax
可接受价格(必须明码标价！):
$30 一套
物品新旧要求:
New
两种礼包：
礼包1（7-pieces gift from dillard's）22个 ：
Cosmetic bag, mascara, lipstick, moisturizer（小黄油）, facial scrub
and YOUR CHOICE of eye shadow compact/lip gloss set in
warm or cools shades.
礼包2 (7 pieces gift from macy's)8个：
2-in-1 Lipstick & Gloss Duo, Color Compact, Take the Day off Makeup Remover,
Dramatically Different Moisturizing Lotion, Lash Doubling Mascara, Floral
Cosmetic Bag and matching ... 阅读全帖 |
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