q***q
发帖数: 3356 | 1 这篇文章读起来很喜庆啊，跟央视的新闻联播有异曲同工之妙，大概是Gibson自己写的
吧？ |
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q***q
发帖数: 3356 | 3 Gibson现在贵的太不正常，当然Les Paul也被吹的太高。我觉得SG standard还算对路。 |
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q***q
发帖数: 3356 | 4 呵呵，Gibson的市场部门真的可能搞这个噱头。 |
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l*y
发帖数: 21010 | 6 You buy a Squier and a Epiphone to find out!!
total cost: around $800.
I bought a Squier Jazzmaster (J Mascis signature style)
and a Epiphone G400 SG.
I'm a Gibson guy! |
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h*******g
发帖数: 10585 | 7 不算对焦这种
纯看画质的话，Leica在135里应该就是Gibson在吉他界的地位了
如果摄影师可以公众化，偶像化
那我估计摄影界中的巨星应该手持Leica |
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w*****u
发帖数: 2 | 10 请教各位：在BALTIMORE，DC 和NEW YORK 附近有好的乐器商店吗？老公想买把FENDER
或GIBSON 的电吉它。
另外， 有谁知道8月间在上述地区有摇滚音乐会吗？
多谢各位XDJM |
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t*******n
发帖数: 4445 | 13 From
http://www.nytimes.com/2003/06/25/opinion/25GIBS.html?th
June 25, 2003 Op-Ed
The Road to Oceania, By WILLIAM GIBSON
VANCOUVER, British Columbia
Walking along Henrietta Street recently, by London's Covent Garden, looking
for a restaurant, I found myself thinking of George Orwell. Victor Gollancz
Ltd., publisher of Orwell's early work, had its offices there in 1984, when
the company published my first novel, a novel of an imagined future.
At the time, I felt I had lived most of my life under |
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M***e
发帖数: 133 | 14 在perception theory里面，流行的观点是（unconscious）information
processing，认为感官刺激传入中枢神经系统，和我们关于这个世界的知识
结合起来，形成知觉。然而Gibson的观点与众不同，认为和知觉有关的信息都
已经包括在感官刺激之中。对于三维世界的感知，他的optical flow的分析是
很经典的。但他进一步说，物体的功用 （ physical affordance ) 也是
如此，比如苹果可以吃，笔可以拿来写字，等等。这儿有个经典的反例，就是
邮筒和垃圾箱，形状大小可以类似，但是功用完全不同。
这两天下雨，屋子里闹蚂蚁，昨天蚂蚁爬满了我的垃圾桶。于是我把里面东西
倒了，把桶放到了门外。
今天回来一看，UPS把我的包裹扔在垃圾桶里了。 |
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d***y
发帖数: 8536 | 15 最近在構建一個非常狗血的質粒，想試試gibson assembly。看官們對這個方法有啥評
價？ 成功率高嗎？ |
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x********u
发帖数: 430 | 16 Follow the NEB Gibson assembly protocol. It's pretty cool.
You might also want to try Red/ET recombination provided by Gene Bridge. |
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h**********r
发帖数: 671 | 17 Perhaps we will get Gibson assembly kit from NEB. Thank you so much for your
info! |
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g*********5
发帖数: 2533 | 18 Red/ET need 50nt and Gibson 15bp nt...
Red/ET is good for mice target vectors? |
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y********8
发帖数: 23 | 19 做Gibson Assembly把两个DNA片段，分别是（5K和4.8K）连接进2.3kb的backbone，
但是每次的测序的结果都是两个DNA insertion（5K和4.8K）中间都会缺失很大一个片
段，但是和backbone overlap的片段都在，实在想不出来为什么会这样，所以想求教大
家。 谢谢！ |
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w********a
发帖数: 324 | 20 大家都在用Gibson Assembly吗？
大家的vector backbone和insert都是切胶纯化的吗？怎么发现切胶后，效率很低，转
到大肠杆菌以后一般很难得到colony。
大家遇到同样的情况吗？有经验的说说。
非常感谢！ |
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x********e
发帖数: 35261 | 21 据版上各位分子克隆大神说Gibson百发百中，正好我要克隆几个比较大的enhancer到9k
的质粒里，就试了一下。positive control连接转化没问题，但是我自己设计引物p出来
的片段跟载体死活连不上。是不是我的引物设计有问题？ |
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M******g
发帖数: 152 | 22 I've used Gibson Assembly more than twenty times and had no problem at all.
Tell me more details. I might be able to help.
how did you open up your vector? PCR or restriction enzymes?
It is better if you purify the pcr product.
don't use competent cells other than NEB. for some reason, it didn't work
well if use invitrogen cells etc.
Dh5alpha comp cell from NEB is better than NEB10 beta regarding
transformation efficiency. |
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w*e
发帖数: 740 | 23 我原来做头几次也没问题
后来做复杂的质粒就出问题了，效率很低
请你来讲讲你的经验呀
I've used Gibson Assembly more than twenty times and had no problem at all.
Tell me more details. I might be able to help.
how did you open up your vector? PCR or restriction enzymes?
It is better if you purify the pcr product.
don't use competent cells other than NEB. for some reason, it didn't work
well if use invitrogen cells etc.
Dh5alpha comp cell from NEB is better than NEB10 beta regarding
transformation efficiency. |
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x********e
发帖数: 35261 | 24 你可以去网上搜Gibson cloning的原始paper, mix的主要成分就是T5和Phusion. NEB就
是改进了下比例。有人直接用那篇paper上给的recipe配,也能做 |
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H****N
发帖数: 997 | 25 Can anybody share you experience with Gibson assembly? Planning to do it,
but would like to hear your tips and potential pitfalls with the technique.
Thanks in advance. |
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M******g
发帖数: 152 | 26 Just use NEB gibson assembly kit or their newer version NEBuilder hifi DNA
assembly kit. you can clone up to 6 fragments. Make sure use their
competent cells, since the assembly rxn many times is not compatible with
competent cells from other companies such as invitrogen or homemade cells.
you can use NEBuilder software to design your primers: http://nebuilder.neb.com/.
I have assembled 4 fragments and plasmids are normally 7-8 kb. if you have
questions, just call NEB tech support, and they ... 阅读全帖 |
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l*******m
发帖数: 3346 | 28 然后NPR昨天的听众反馈全是对NPR居然播放这段录音表示愤怒的，主持人说大家对Mel
Gibson的愤怒显然没有对我们这个coverage来的多，就记得一个听众留言说：Is that
news? |
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H*****r
发帖数: 764 | 29 Scout details relationship with Oregon, Kelly
HOUSTON – Embattled scouting service owner Will Lyles told Yahoo! Sports
that University of Oregon coach Chip Kelly personally approved a
controversial $25,000 fee that sparked an ongoing NCAA investigation and was
in constant contact as Lyles provided the Ducks with recruiting assistance
that may have violated NCAA rules.
Will Lyles stands with running back recruits Dontae Williams (20) and Lache
Seastrunk (15) after the Aloha Bowl on Dec. 12, 2009.... 阅读全帖 |
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y***r
发帖数: 65 | 30 1958 Harvey Haddix, Pittsburgh
1959 Harvey Haddix, Pittsburgh
1960 Harvey Haddix, Pittsburgh
1961 Bobby Shantz, Pittsburgh
1962 Bobby Shantz, Houston-St. L
1963 Bobby Shantz, St. Louis
1964 Bobby Shantz, St. L-Chi-Phil
1965 Bob Gibson, St. Louis
1966 Bob Gibson, St. Louis
1967 Bob Gibson, St. Louis
1968 Bob Gibson, St. Louis
1969 Bob Gibson, St. Louis
1970 Bob Gibson, St. Louis
1971 Bob Gibson, St. Louis
1972 Bob Gibson, St. Louis
1973 Bob Gibson, St. Louis
1974 Andy Messersmith, |
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f*******l
发帖数: 8811 | 31 Gibson met Robyn Denise Moore in the late 1970s soon after filming Mad
Max when they were both tenants at a house in Adelaide. At the time,
Robyn was a dental nurse and Mel was an unknown actor working for the
South Australian Theatre Company.[82] On June 7, 1980, they were marri
ed in a Catholic church in Forestville, New South Wales and she became
known as Robyn Gibson.[83] The couple have one daughter, six sons, an
d two grandchildren.[84]
After 26 years of marriage, the couple separated in A... 阅读全帖 |
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l****z
发帖数: 29846 | 32 April 13, 2012 by Warner Todd Huston
Left-wingers have for years complained that conservatives are wrong to
condemn a movie based on the outspoken nature or politics of a staring actor
or director.
“It’s just a movie,” they tell us and they scold us for not separating
the entertainment from the actor’s real life. But now, in a typical
hypocritical move, left-wingers are condemning a movie that is in the works
precisely because of who is involved in the project, Mel Gibson, someone
they do not ... 阅读全帖 |
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f**********n
发帖数: 29853 | 33 奇怪，莫名被删了。幸好俺屏幕还在。
...and they then confiscated approx. $1million in costly exotic wood (teak,
ebony, etc), effectively shutting down the Gibson factories for a month.
They cited an obscure law from 1900 (the Lacey Act) and claimed Gibson MAY
BE AT RISK of violating laws of Madagascar and/or India. (not U.S. law).
They provided no evidence.
Gibson CEO Henry Juszkiewicz was a prominent supporter of Conservative
causes and had donated to Rep. Marsha Blackburn and Sen. Lamar Alexander.
The raid cost... 阅读全帖 |
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H*****r
发帖数: 764 | 34 May 12, 2011
osu_tresseldebut_inline051211_ap
Click here for a timeline and photos of Jim Tressel's tenure at Ohio State.
Only an optimist would smash one ant scurrying from beneath the rug and
breathe a sigh of relief over having eradicated the ant problem.
Only an optimist.
Or an Ohio State fan.
Since the Tattoo-Gate scandal broke in late December, then mushroomed into
the fireball of head coach Jim Tressel’s lying to the NCAA and OSU, a
segment of Buckeye supporters has seized upon every figu... 阅读全帖 |
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b*s
发帖数: 82482 | 35 Neal Stephenson和《Reamde》
我看Neal Stephenson的书有些年头了。最早知道这么个作家，是在读Slashdot网站的书评的时候。毫无疑问，Neal Stephenson是Geek和Nerd的所喜闻乐见的小说家。早期的《Snow Crash》，出版于1982年，就预见到了全球性的光缆交互式互联网游戏，可以算是World of Warcraft的前身。而且，里面所描述的虚拟地球，明明就是Google Earth嘛。后来，1995年出版的《Diamond Age》,则更精彩了，对于Nano科技，以及对于Steampunk题材的探索，都是跨时代性质的。在《Diamond Age》里，一大部分的情节发生在一个虚构的亚洲，中国分裂成沿岸共和国和内陆共和国，海外有人工大陆新亚特兰蒂斯，一个小女孩卷入了上海和新亚特兰蒂斯之间的一个连环扣阴谋…
后来，1999年，Neal出版了巨著《Cryptonomicon》，探索科技，货币，黑客，二战等主题，而且，也是错综复杂的情节，精辟的科技Infodump，这是他作品的一大特色。这部小说可以说是一本巨著，有上千页。最后，关于网络加... 阅读全帖 |
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b*s
发帖数: 82482 | 36 Neal Stephenson和《Reamde》
我看Neal Stephenson的书有些年头了。最早知道这么个作家，是在读Slashdot网站的书评的时候。毫无疑问，Neal Stephenson是Geek和Nerd的所喜闻乐见的小说家。早期的《Snow Crash》，出版于1982年，就预见到了全球性的光缆交互式互联网游戏，可以算是World of Warcraft的前身。而且，里面所描述的虚拟地球，明明就是Google Earth嘛。后来，1995年出版的《Diamond Age》,则更精彩了，对于Nano科技，以及对于Steampunk题材的探索，都是跨时代性质的。在《Diamond Age》里，一大部分的情节发生在一个虚构的亚洲，中国分裂成沿岸共和国和内陆共和国，海外有人工大陆新亚特兰蒂斯，一个小女孩卷入了上海和新亚特兰蒂斯之间的一个连环扣阴谋…
后来，1999年，Neal出版了巨著《Cryptonomicon》，探索科技，货币，黑客，二战等主题，而且，也是错综复杂的情节，精辟的科技Infodump，这是他作品的一大特色。这部小说可以说是一本巨著，有上千页。最后，关于网络加... 阅读全帖 |
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H*****r
发帖数: 764 | 37 A full quote from Dave Williford given on Wednesday when we made our call to
the UO AD's office. The last sentence had been removed from final edit.
"I can't confirm or deny that. Because that wasn't something I was aware of."
Multiple sources close to the Oregon football program tell DuckTerritory.com
that Assistant Director of Football Operations Josh Gibson believed to be
no longer working in the football office. It is unclear if Gibson has been
fired, if he resigned or if he has been reassig... 阅读全帖 |
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g*******x
发帖数: 2158 | 38 其实作者并没有给出太多的statement。
作者是Eirik Johnson.这个主题是一组名为animal holes的组片。下面是一点说明
There is something sad about these landscapes. They are, after all, located
in the neglected empty space which exists between public and private land.
Yet, these spaces also offer a constant reminder of the resilience of nature
. This theme of ecology adapting to change was what inspired my next
project, Animal Holes. It was on one of my urban expeditions along an old
landfill in California that I came upon a warren of fer... 阅读全帖 |
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l****z
发帖数: 29846 | 39 班加西丑闻：美国驻利比亚副大使Gregory Hicks证词与奥巴马政府相反，他指出特种
部队在恐怖袭击时准备飞往班加西救援，但被军方制止
Diplomat: U.S. Special Forces told "you can't go" to Benghazi during attacks
U.S. Ambassador Chris Stevens and three other Americans were killed in the
consulate attack in Benghazi, Libya on Sept. 11, 2012.
The deputy of slain U.S. Ambassador Christopher Stevens has told
congressional investigators that a team of Special Forces prepared to fly
from Tripoli to Benghazi during the Sept. 11, 2012 attacks was forbidden
from doing ... 阅读全帖 |
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l****e
发帖数: 609 | 40 我只是想为楼主解答“为什么美国女性很多支持希拉里”,澄清misleading的指责。那
是40年前的事，那时刚出道的她只有27岁。之前和之后她还为妇女儿童做了很多事情，
有人如果要从这件事来证明她毫无人性,那是选错了证据。
But did Hillary try to get out of case?
Gibson, the Washington County prosecutor and one of the only people still
alive with knowledge of the case, has said emphatically over the years that
she did.
In 2008, Gibson told Newsday that "Hillary told me she didn’t want to take
that case. She made that very clear."
Recently, Gibson made similar comments to CNN. He said the judge, Maupin
Cum... 阅读全帖 |
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a*****g
发帖数: 19398 | 41 The one-room schoolhouse, that symbol of rural American education that dates
back to the earliest days of the Colonial era, might be on the verge of
making a comeback.
In recent years, a smattering of "micro schools" have popped up in places
such as California's Silicon Valley; Austin, Texas; and New Orleans,
offering parents a drastically different version of K-12 education than
traditional public and private schools. These are tiny schools—sometimes
with as few as half a dozen students—that pu... 阅读全帖 |
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n*****u
发帖数: 465 | 42 这房子的价钱史
05/10/16 Price change $1,198,000-6.0% $276 Keller William...
04/20/16 Price change $1,275,000-1.8% $294 Keller William...
01/21/16 Listed for sale $1,299,000-18.3% $300 Gibson Sotheby...
10/07/15 Listing removed $1,589,000 $366 Gibson Sotheby...
06/22/15 Price change $1,589,000+6.0% $366 Gibson Sotheby...
06/16/15 Price change $1,499,000-5.7% $346 Gibson Sotheby...
06/07/15 Listed for sale $1... 阅读全帖 |
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G****a
发帖数: 10208 | 43 圣路易红雀 （ St. Louis Cardinals ），是美国职棒大联盟中，隶属于国家联盟的棒
球队伍之一。 主场位于密苏里州的圣路易斯 。 在国家联盟的分区中，属于国家联盟
中区 。 他们总计共获得10次世界大赛冠军，在国家联盟是获得最多冠军的球队。 在
大联盟的30个球队里面，仅次于纽约洋基 （获得27次世界大赛冠军）。
红雀最早是创立于1882年，当时 的联盟为美国协会 ，球队取名为圣路
易棕长袜（St. Louis Brown Stockings），之后在1892年加入国家联盟 ，并在1900年
改名为现在大家所熟悉的队名圣路易红雀。 红雀现所使用的主场为布希体育场（Busch
Stadium），于2006年开始启用，在启用的第1年，红雀便夺下该年的世界大赛冠军，
上一次在主场启用第1年便夺下世界大赛冠军的是1923年的洋基（最近一次是2009年的
洋基体育场 ）。
球队历史
球队创立
1881年，运动家球场俱乐部(Sportsman's Park andClub)组成了圣路易布朗队(St.
Louis Browns)，并加入甫成立的美国协会 ... 阅读全帖 |
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a*t
发帖数: 2596 | 44 Slash的武器
金银财宝(2000-11-28)
喜爱重金属摇滚的朋友们，一定听过在80年代风靡一时的Guns'n
'Roses (枪与玫瑰乐队),Guns'n'Roses有着不少张脍炙人口的作品，
AXL ROSE更是个问题人物，倍受乐迷瞩目，而我们广大喜爱吉他的朋
友们对Guns'n'Roses情有独中的原因是——伟大的吉他手SLASH！
SLASH让人哭泣的吉他演奏、光芒四射的舞台身影、大礼帽、永
远盖住脸的发型……所有的一切都这样让我们着迷。还有他手中那把
在“十一月的雨中”淋湿了所有少女和我们的心的GIBSON LesPaul
classic系列电吉他。
如火焰后晚霞的颜色是GIBSON沿用多年的经典琴身样板，看上去
如坏脾气的女人。两块双拾音器的平行排列，0.010的韧劲琴弦，三
段调节选择开关，较厚的琴颈设计，无摇把系统的简洁琴身，就是这
些在接通了MASHALL音响，打开失真通道后我们就听到了让我们哭泣
的‘don't cry’。
一般来说，GIBSON除了‘夜莺’系列外，les
paul系列，classic系列，spangarg系列等等都是为主音型吉他手所
设计的。... 阅读全帖 |
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p*******m
发帖数: 20761 | 45 Steve Gibson's Three Router Solution to IOT Insecurity
Author: Nicolae Crisan
Date: August 15, 2016
Subject: General Tech
Manufacturer: Various
Tagged: networking, network, iot
Introduction
Even before the formulation of the term "Internet of things", Steve Gibson
proposed home networking topology changes designed to deal with this new
looming security threat. Unfortunately, little or no thought is given to the
security aspects of the devices in this rapidly growing market.
One of Steve's propos... 阅读全帖 |
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a*****g
发帖数: 19398 | 46 The one-room schoolhouse, that symbol of rural American education that dates
back to the earliest days of the Colonial era, might be on the verge of
making a comeback.
In recent years, a smattering of "micro schools" have popped up in places
such as California's Silicon Valley; Austin, Texas; and New Orleans,
offering parents a drastically different version of K-12 education than
traditional public and private schools. These are tiny schools—sometimes
with as few as half a dozen students—that pu... 阅读全帖 |
|
s*******u
发帖数: 2249 | 47 Palin’s Definition of ‘Bush Doctrine’ Hits the Gibson Mark
By Bill Sammon
ABC News’ Charles Gibson, who is being credited with stumping Sarah Palin
on the definition of the “Bush Doctrine,” has himself defined the nebulous
phrase in a variety of ways, including one that mirrored Palin’s disputed
explanation.
Gibson and his colleagues have been all over the map in defining the Bush
Doctrine over the last seven years. In 2001, Gibson himself defined it as “
a promise that all terrorists organizati |
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l****z
发帖数: 29846 | 48 by Jeffrey Lord
Twinkicide.
Death by Liberalism.
This time it's Twinkies.
In October it was Newsweek.
Who's next? What does this lemming-like liberal instinct mean for America?
What is it that bakers and editors had in common as they looked into the
abyss that would spell their own professional death? And then jumped?
Answer: They'd rather be dead than Not Liberal. Unfortunately for the rest
of us -- they are not alone. Worse, they are in charge.
Presented with multiple opportunities to survive ... 阅读全帖 |
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