n*********0
发帖数: 925 | 1 http://www.glorypress.com/devotional/FaithAndLifeOneYearBook.asp?bid=3
倾听信号
经文: 「你听见桑树梢上有脚步的声音，就要急速前去，因为那时耶和华已经在你前头
去攻打非利士人的军队。」（撒母耳记下五︰24）
上帝行动的信号，祂可以让我们感受得到，圣灵会向侧耳倾听的信徒说话，因
此我们要比以往更加警觉，抓住宝贵的机会，并善加利用。和非利士人争战是我们的责
任，但是，当上帝自己走在我们面前，我们就应更加勇敢。
微风响过树梢，大卫和他的侍从将此当做杀戮非利士人的记号，而且上帝自己
要走在他们前面，为他们开路。上帝今天或许就要用你做祂的出口，为祂说话。让我们
儆醒，侧耳倾听响过树梢的风声，顺服内心圣灵的感动，焉知现在不是个丰收的季节﹖
上帝不是如此应许﹖ 「祂要走在我前头。」这是何等大的鼓励！我们尽管勇往直前，
切莫退缩。
Listen for the Signal
Scripture: "And let it be, when thou hearest the sound of a goi |
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R*o
发帖数: 3781 | 2 By Pastor Kelly Sensenig
The postmillennial view of the Millennial Kingdom was all but extinct but in
the last 35 to 40 years there has been a resurgence in this concept and vie
w of the Millennial Kingdom. This system of millennial thought was developed
in the 16th century. The view holds that conditions on earth will gradually
improve over time evolving into a world that has become "Christianized" and
ready for the Second Coming of Christ to start eternity. The prefix "post"
before Millennium ... 阅读全帖 |
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R*o
发帖数: 3781 | 3 By Pastor Kelly Sensenig
Similar to Amillennialism, this view claims that the kingdom of Revelation 2
0:4 is primarily Christ ruling in the hearts of His people (the Church). Acc
ording to the postmillennialist this reference does not refer to a literal t
ime when Christ will return to earth and set up a kingdom since the church i
s said to set up the kingdom as a result of Christ ruling in their hearts. R
evelation 20:4 needs to be interpreted figuratively in this system to once a
gain mean tha... 阅读全帖 |
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R*o
发帖数: 3781 | 4 but a person shall not betray his own ancestors only for the benifit of "goi
ng to heaven"
see what Paul said 罗马书 9：3. 为我弟兄，我骨肉之亲，就是自己被咒诅，与基督
分离，我也愿意 . |
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w**********1
发帖数: 2588 | 5
“愿意”只是 主观上的愿望, 说明他对弟兄的爱。
但Paul自己也说过，没有什么可以让他跟主的爱分离.
神也是 own ancestor.
另，自己的先人，如果一生没有机会接受福音，
也许会再有千年国的机会.
goi |
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R*o
发帖数: 3781 | 6 4:18 Verse 18 begins the second part of this paragraph in which Paul will la
y great stress on the nature of Abraham’s faith. There was no hope—i.e., i
t was against [all] hope (par* ejlpivda, par elpida) on a human level—of th
e fulfillment of the promise God gave to Abraham. But the patriarch’s hope
was not in himself or his wife or anything human; it was in God and his powe
r. Abraham believed in hope (ejp * ejlpivdi ejpivsteusen, ep elpidi episteus
en) with the result that the promise was fu... 阅读全帖 |
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R*o
发帖数: 3781 | 7 H. Contribution of Passage to Discipleship and Church Mission
There are many principles that could be gleaned from this passage for discip
leship and church mission. We will mention only one: the idea of a deep, abi
ding faith in God. The kind of faith that pleases God is a faith that takes
him at his word and trusts in his promises without question (Heb 11:6). Abra
ham did not have any human assurance that what God promised was actually goi
ng to happen. He didn’t have the assurance of an inscr... 阅读全帖 |
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e*****n
发帖数: 244 | 8 hi friends,
who is currently a Ph.D. candidate?
it is not so easy.
how is verything goiing, friends?
I went back to China a month ago. Beijing, Shi Jia Zhuang
and Xian, all the cities change a lot and deeply. it is very
nice to notice that the country is developing so quickly.
Elysian |
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g*******a
发帖数: 31586 | 9 过年干什么？过年吃鸡。（吃鸡怎么说实在想不起来了。。。求教！）
假goi
我出拼不出 |
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S****y
发帖数: 17 | 10 我先说两个:
姜嘎lo，滴galore；
阿Hia怕阿do，阿do倒lo棒，阿hia吉goi郎。
译文:幸灾乐祸，四簸箕，阿兄打阿嫂，阿嫂跑进房，阿兄织鸡笼。
麻利舍，利宋ho，搞逮莫，呗ddoddo.
译文: 剪刀石头布，花生糖，狗屎毛，拔得长。 |
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u**********e
发帖数: 282 | 11 In JUnit, I use easyMock to create mock object. However it throws exception:
ava.lang.IllegalStateException: missing behavior definition for the
preceeding method call getSubName()
at org.easymock.internal.MockInvocationHandler.invoke(
MockInvocationHandler.java:27)
at org.easymock.internal.ObjectMethodsFilter.invoke(ObjectMethodsFilter.
java:45)
at $Proxy0.getSubName(Unknown Source)
at test.rms.TestDefaultDataPublisher.setUp(TestDefaultDataPublisher.java
Anyone knows what's goi |
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m*********7
发帖数: 5207 | 12 能不能设计siRNA只针对GFP，而不是GOI？
这样的siRNA会有效果吗？ |
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m*****n
发帖数: 760 | 13 if this works, that would be exactly what I want.
in this way, I can determine if the phenotypes
are induced specificly by GFP-GOI transgene. |
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O******e
发帖数: 4845 | 14 他应该是想用siRNA去除GFP-GOI to verify their GFP signals (localization
pattherns, maybe?). |
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w********r
发帖数: 1431 | 15 lz发个包子吧，哈哈
To deliver siRNA/cDNA into muscle of adult mice, the TA muscles were
pretreated with 30 ul of hyaluronidase (0.4 U/ml) (Sigma) 2 hr before siRNA/
cDNA injection. For each TA muscle, 6 ul of siRNA (50 uM) (or several ug of
plasmid. the amount of plasmid depends on the expression of your GOI) was
diluted with 0.9% NaCl to a final volume of 30 ul, and injected into the TA
muscle and followed with electroporation using a BTX ECM 830 generator (mode
a pair of 7 mm Tweezertrodes (BTX) with ... 阅读全帖 |
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w********r
发帖数: 1431 | 16 RNAi出现的一个重要意义就是可以让大家跳过KO，
而用便宜省时间的方式检验GOI loss of function的作用。
但通常手里有KO的还是要用KO再show off一下，
reviewer们喜欢看这个 |
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m******t
发帖数: 109 | 17 我把GOI 插入到一个带GFP的空载体里面，结果发现不绿，而同时转的原来的 空载体还
是绿的。PLASMID 是SEQUENCE过的。求教了 |
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O******e
发帖数: 4845 | 18 (TIME.com) -- It's called ibrutinib, and it's a potential breakthrough in tr
eating chronic lymphocytic leukemia (CLL) that could leave patients with few
er side effects than chemotherapy.
In research published in the New England Journal of Medicine (NEJM), scienti
sts report that the experimental drug, which differs from broadly acting che
motherapy agents by specifically targeting certain cancer-causing processes,
significantly prolongs the life of patients.
Ibrutinib is currently being tested... 阅读全帖 |
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m******5
发帖数: 1383 | 19 只是想挖几个现有的发表过的Chip-Seq 看几个TFs对GOI的调控
请问新手上路用哪个broswer好？
galaxy怎么样？ |
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j*****q
发帖数: 82 | 20 Can start with AA or DNA sequence or plasmid. GOI will be cloned into in
house vectors.
For quotation, pls PM. Price should be very compectitive. |
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s*****i
发帖数: 315 | 21 some times knockout first may have leakage, and still result in GOI
expression.
Breeding with Cre makes it is a real knockout.
FRT makes it conditional.
and
is |
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H*******i
发帖数: 196 | 22 在给定系统（比如某种yeast或者特定cell line），在给定构建基础上（promoter 5'
UTR GOI， 其他可能存在的调控元件可以先放弃），可以定量确定如何调整基因表达（
通过控制这些部分的序列和构建）。
进一步，逐渐在系统里加入调控元件，来确定如何构建可调控系统。
现在已有的基础是什么程度？有没有review文章可以借鉴下
度？ |
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a*****g
发帖数: 19398 | 23 By Eric Schulzke , Deseret News National Edition
Published: Thursday, Jan. 22 2015 7:00 a.m. MST
While research shows the benefits, in-school and after-school programs del
iver the goods. (DimaSobko, Getty Images/iStockphoto)
The first time children called her "The Chess Lady" in a Seattle school 10 y
ears ago, Wendi Fischer was caught off guard. As she walked in, they started
buzzing, and when she asked the teacher what they were saying, it turned ou
t she was a minor celebrity. The children ... 阅读全帖 |
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le
发帖数: 190 | 24 Exactly! University Ranking is the least important thing.
The most important things are:
1. what you are goiing to do
2. how easily you can graduate and land on a job |
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K***6
发帖数: 155 | 25 Thanks for your input!
However, I don't hear many people doing post-doc in industry. Is it because
post-doc is mainly for people who eventually want to land a job in academia,
so post-doc in industry is not as good as the ones at school?
In other words, how is "post-doc in industry" defined here? In my opinion, "
post-doc" is something temporary. Why do companies bother to offer such a
position if there is no real job opening. Companies do not have obligation
to train a post-doc if he/she is goi |
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l*****i
发帖数: 3929 | 26 In the second case, have you noticed the "probability" of the underlying goi
ng to 24? |
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p********0
发帖数: 186 | 27 Thanks, it seems that the risk neutral decide the p, the p(probability going
to 24) become much smaller 0.15 compare to 22(0.45).
goi |
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t*****b
发帖数: 157 | 28 I am busy this week so didn't get time to take pic.
Here is the link for the new 2010 Jetta TDI sportwagen, except Mine is Candy
White. I believe that mine looks better. Acturely I like the 2009 front
face better (the 2010 sedan still uses old face), the new one looks so
aggressive..
http://www.autoblog.com/2009/05/29/vw-of-america-confirms-that-2010-jetta-sportwagen-gets-new-face/
no need too much upgrade, the standard is already very good. I could also
expect $1300 tax credit for this car.
Goi |
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p****e
发帖数: 1028 | 29 说强队说得有点腻了，换成劲旅吧。。。
比较平均的队伍，啥都拿得出手。即侯赛前途要看对手的类型了。。。q今年会goi
ng up, 因为不可能going downer乐。。。 |
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