h******u
发帖数: 602 | 1 检测一单链DNA是否形成HAIRPIN， 是不是Nuclease S1就是做好的方法了？初步打算用
Cy3-label 5'end，然后用Nuclease S1处理，如果形成HAIRPIN的话，LOOP就会被降解
，产物RUN PAGE GEL。
从来没用过Nuclease S1,有啥要注意的吗？谢谢了！ |
|
|
k****n
发帖数: 42 | 3 刚从虫子转到哺乳系统， 大家一般都是从哪个网站去找一个基因的promoter的？
然后有没有一些网站会帮你设计shRNA hairpins 序列的？ 谢谢了 |
|
l**********1
发帖数: 5204 | 4 //biowulf.bu.edu/zlab/PromoSer/
>哺乳系统， 哪个网站去找一个基因的promoter的？
----
设计shRNA hairpins 序列的？
//codex.cshl.org/RNAi_central/RNAi.cgi?type=shRNA
//codex.cshl.org/research.php
all hints from
2008 SUNY one PhD dissertation
full text pdf link:
//dspace.sunyconnect.suny.edu/bitstream/handle/1951/47599/000000431.sbu.pdf?sequence=3 |
|
d****u
发帖数: 1553 | 5 策略大概是这样的，首先假定这是一个negative selection， 筛选marker是细胞的生
长速度。我们用一个pooled shRNA library（好比说有100K hairpins）去转染或感染
细胞。那么经过一段时间的细胞分裂之后（让我们假定一个月），有些hairpin会使细
胞增长加速，有些hairpin会延缓细胞生长。从表象上看就是在整个细胞群体中，有些
hairpin被富集了，有些被降低了。这时候就可以收细胞提DNA，然后PCR扩增hairpin
sequence。然后或者上array或者上nextgen sequencing。两者的read out都是hairpin
在整体中的丰度。然后再normalize to 第一天的data。就能算出哪些hairpin可以促进
或抑制细胞生长了。我的问题就是这时候做PCR如何保证整体里不同的hairpin是线性扩
增使得最后的read out能够进行比较。我的理解是这时候不管是array或sequencing都
不能让你知道在整个population里那个基因被knockdown了。而是告诉你你的这个DNA中
有哪些ha... 阅读全帖 |
|
d****u
发帖数: 1553 | 6 谢谢回复，你说的可能是针对单一基因的knockdown而不是针对screen的。做基因组级
别的pooled RNAi screen的时候，在筛选结束要抽基因组DNA。然后PCR扩增hairpin
sequence。再去上array或sequencing，就可以知道哪些hairpin富集了，哪些hairpin
减少了。 我问的是这一步，这时候因为所有的不同基因的hairpin都是混在一起的，所
以不可能得到特定基因的knock down水平 |
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x*****o
发帖数: 441 | 7 Mg 有效的中和RNA backbone 的负电荷,可以稳定二,三级结构. Na或者K没有Mg有效,
一般认为稳定的是RNA的二级结构.如果在没有镁的情况下,大量的单价离子 (1M)可以减
少 non-specific binding,稳定特定的conformation. 但是不知道NMR是不是不能用大
量的单价离子.
我们实验室以前也用hairpin(虽然不是核磁)hairpin ribozyme, 但是觉得mg 在10mM
的时候效果最好.
我以前有朋友做RNA NMR, 也是hairpin,不过他们的stem是GC rich的,比较稳定. |
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M***D
发帖数: 249 | 8 Antipsychotics: A New Hope Against Glioblastoma
Researchers have discovered that FDA-approved antipsychotic drugs possess
tumor-killing activity against the most aggressive form of primary brain
cancer, glioblastoma.
The team of scientists from the University of California, San Diego School
of Medicine, led by principal investigator Clark C. Chen, MD, PhD, used a
technology platform called shRNA to test how each gene in the human genome
contributed to glioblastoma growth.
ShRNA stands for small ... 阅读全帖 |
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t****v
发帖数: 9235 | 9 对个屁
都是死读书
在直行车道上行人应该逆行
但是在hairpin小弯道上，行人应该走在外侧，弧度大空间大容易避让
hairpin不放一个镜子，司机什么都看不到 |
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t****v
发帖数: 9235 | 10 对个屁
都是死读书
在直行车道上行人应该逆行
但是在hairpin小弯道上，行人应该走在外侧，弧度大空间大容易避让
hairpin不放一个镜子，司机什么都看不到 |
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S**U
发帖数: 7025 | 11 I think the term is spinning net shot or hairpin net shot. Hairpin refers to
the trajectory, and spinning refers to the movement of the shuttle. |
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B********g
发帖数: 6 | 12 Nobody will 歧视 you unless you 歧视 yourself.
You are yourself. Why do you worry about how people looked at you?
You can speak English? Can English speaking people speak Chinese as well as
you?
Learn to 尊重 yourself first.
Here is a story:
There was a little girl who was born in a very poor family. She can't afford
to buy any new cloth, new make up etc. Mom gave her ONE dollar for
Christmas. She said: "I am going to buy a beautiful hair pin to attend my
school party." She didn't want to walk in the ... 阅读全帖 |
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t*******d
发帖数: 2570 | 13 试图更押韵一些的尝试。
I am the princess of the underground kingdom and my name is Camilla.
I have black eyes and hair darker than the darkest sash.
You are the prince of the light world, bright and shining, with purple
primrose on your robe.
The first time I lay my eyes on you, is that early summer.
We were thirteen and it’s the first time I left the underground kingdom.
The first time to see the blue bellflower;
The first time to see the reflection of white swans on the mirror-like lake;
And the first ti... 阅读全帖 |
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O******e
发帖数: 4845 | 14 你这是在拼概率。
干嘛不去做个microarray，直接知道哪些基因的表达被下调，比你知道哪些hairpin被
富集直接多了。不过就是比较贵，呵呵
hairpin |
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c******r
发帖数: 3778 | 15 明白了
这个是没有办法的事情。PCR扩增确实可能产生系统误差，但是目前没有办法完全消除
这个误差。当然楼上也说了，这么小的hairpin，又是唯一的primer，应该效率是差不
多的，这个系统误差应该不大。
所以这个PCR之后的只是筛选，挑出可能的candidate，然后还需要其他方法进一步确认
。比如你挑了10个candidates，可能只有3个被确认，也可能有9个被确认，这个就看你
下一步怎么弄了。
hairpin |
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p*****y
发帖数: 44 | 16 你担心的是PCR效率问题。但是在做screen的时候，所有样品都是用同样的条件进行，
所以误差可以忽略。线性的问题主要决定于hairpin在不同样品里的浓度，以及你PCR的
循环数。最佳的条件当然是PCR Template在每个样品之间都一致，这样在同一个循环结
束。告诉你一个Trick,我都是先用Real-time PCR定量每个样品的hairpin浓度，然后调
整到每个样品一致。再根据Rreal-time PCR选择最后一个还大致处于线性扩增的循环结
束。因为Microarray, Sequencing都要很多PCR产物，做两轮PCR会更好。 |
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s*********y
发帖数: 387 | 17 thanks.
I would say the points of 1 and 3 are really fine if we just want to
identify some targets, instead of a thorough screening.
Can you help explain more on
"2nd, readout, both microarray and deep sequencing are available,
however, if
you can not avoid PCR on the hairpin, you will always have difficulty in
the secondary structure."
you mean the PCR for the hairpin sequence will be difficult?
available
some
however, if
in
genes |
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l**********1
发帖数: 5204 | 18 LZ pls try long hairpin RNA (lhRNA)
Reference:
Tomimoto K et al. (2012)
Construction of a long hairpin RNA expression library using Cre recombinase.
J Biotechnol. 160: 129-39.
its pp130 left column
Functional screening using lhRNA expression libraries also appears to
be more efficient than using shRNA expression libraries for these
organisms.
link:
//www.ncbi.nlm.nih.gov/pubmed/22484114 |
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l**********1
发帖数: 5204 | 19 LZ can try long hairpin RNAi
Reference:
Tomimoto K et al. (2012)
Construction of a long hairpin RNA expression library using Cre recombinase.
J Biotechnol. 160: 129-39.
PubMed link:
http://www.ncbi.nlm.nih.gov/pubmed/22484114
发信人: Wanshington (Joudon), 信区: Biology
标 题: Re: 讨论：信号通路上的蛋白酶功能研究策略哪种好？
发信站: BBS 未名空间站 (Fri Sep 28 03:33:37 2012, 美东)
RNAi不是有脱靶的问题吗？ 以前没有做过，所以对这个问题是否严重没有很好的把握
。这个蛋白的磷酸化位点不能突变成模拟磷酸化的蛋白，所以这条道路不通。 |
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h**********t
发帖数: 545 | 20 其實我也干RNAi就三個月，而且還是做的是siRNA...那玩意可控性高多了
理論上說RNAi是比毒蛋白要難控制，實際上現在從植物細胞出來的products是hairpin
結構的單鏈，在昆蟲細胞內被transmittor送進細胞後被昆蟲dicer掉頭發揮作用。
人的針對性RNAi應該更加容易控制，現在人Genome都有了，只要避開某些區域就行了。
其實人能不能吸收RNAi，能不能處理Hairpin結構的RNA，我還不知道，因為不是搞藥的
genome |
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T****u
发帖数: 424 | 21 http://blog.sina.com.cn/s/blog_12d8810790102uyry.html
有微友私信问设计siRNA、shRNA和gRNA. 今天先说siRNA和shRNA吧。
一般我是take advantage of sigma. 他们有现成的shRNA产品，而且反响很好。
当然了，土豪可以自己去Dharmacon订购siRNA， 或者在sigma订购shRNA,甚至是包装好
的病毒。
1.第一步去sigma英文网站，一定要是英文网站
http://www.sigmaaldrich.com/united-states.html
#华夏小奇说实验的事#设计siRNA和shRNA
2. 搜索你感兴趣的基因。如TNFR, 出来结果之后点击shRNA(下图黑色剪头所示)
插播广告 订购基因敲除小鼠请访问公司网站（2-4个月，小鼠转基因、敲除敲入、条件
性敲除）
#华夏小奇说实验的事#设计siRNA和shRNA
3. 点击进入下个页面，点击pricing，会出现每个shRNA的hairpin的序列。
如下面的序列，介于“CCGG”和"CTCGAG"之间的，就是shRNA的目... 阅读全帖 |
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C*********h
发帖数: 83 | 22 有微友私信问设计siRNA、shRNA和gRNA. 今天先说siRNA和shRNA吧。
一般我是take advantage of sigma. 他们有现成的shRNA产品，而且反响很好。
当然了，土豪可以自己去Dharmacon订购siRNA， 或者在sigma订购shRNA,甚至是包装好
的病毒。
1.第一步去sigma英文网站，一定要是英文网站
http://www.sigmaaldrich.com/united-states.html
#华夏小奇说实验的事#设计siRNA和shRNA
2. 搜索你感兴趣的基因。如TNFR, 出来结果之后点击shRNA(下图黑色剪头所示)
插播广告 订购基因敲除小鼠请访问公司网站（2-4个月，小鼠转基因、敲除敲入、条件
性敲除）
#华夏小奇说实验的事#设计siRNA和shRNA
3. 点击进入下个页面，点击pricing，会出现每个shRNA的hairpin的序列。
如下面的序列，介于“CCGG”和"CTCGAG"之间的，就是shRNA的目标序列。
可以根据这个序列设计siRNA和shRNA(自己合成hairpin, 退火连入pLKO or Te... 阅读全帖 |
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L*********d
发帖数: 7037 | 23 别费劲了
春望
杜甫
国破山河在，城春草木深。
感时花溅泪，恨别鸟惊心。
烽火连三月，家书抵万金。
白头搔更短，浑欲不胜簪。
Spring View
Translated by Xu Yuanchong
On war-torn land streams flow and mountains stand;
In vernal town grass and weeds are overgrown.
Grieved over the years, flowers make us shed tears;
Hating to part, hearing birds breaks our heart.
The beacon fire has gone higher and higher;
Words from household are worth their weight in gold.
I cannot bear to scratch my grizzled hair;
It grows too thin to hold a light hairpin. |
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l**k
发帖数: 45267 | 24 americans write it as 11022011, still hairpin |
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l****z
发帖数: 29846 | 25 Previously on The Perils of Pauline:
Last year, our plucky heroine, the wholesome apple-cheeked American republic
, was trapped in an express elevator hurtling out of control toward the debt
ceiling. Would she crash into it? Or would she make some miraculous escape?
Yes! At the very last minute of her white-knuckle thrill ride to her
rendezvous with destiny, she was rescued by Congress’s decision to set up .
. . a Super Committee! Those who can, do. Those who can’t, form a
committee. Those who r... 阅读全帖 |
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D***n
发帖数: 6804 | 26 你多久没看F-1了？2007年 Robert Kubica 以时速300公里速度撞水泥墙，平均过载28G
，瞬间最高过载75G，结果是轻伤。Youtube上有录像
Kubica performed well during the 2007 season, …， in which his car made
contact with Jarno Trulli's Toyota, and hit a hump in the grass which lifted
the car's nose into the air and left him unable to brake or steer. The car
then hit the concrete retaining wall and rolled as it came back across the
track, striking the opposite wall on the outside of the hairpin and coming
to rest on its side.[16] The speed measured w... 阅读全帖 |
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e******g
发帖数: 5344 | 27 这个车为了省油，是最典型的太公四驱。。
The AWD system has a neat trick. In order to reduce "parasitic loss" on the
drivetrain and boost efficiency, the rear axle is completely disconnected at
the transfer case and power unit when all-wheel drive is not needed. When
it is, up to 40 percent of power can go to the rear wheels, or 60 percent in
sport mode. You can feel that power in tight hairpin corners at high speeds
, but otherwise it behaves like a front driver until there's wheel slippage.
跟6缸legacy的VTD+VDC全时四驱系统... 阅读全帖 |
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W***n
发帖数: 11530 | 28 Location: San Juan Mountains
Difficulty: Difficult
Directions: 13 miles outside Ouray on Hwy. 550
Time: Approximately 2-3 hours, one-way. 12 miles.
Trail Description
The infamous Black Bear Pass is one of the most difficult mountain passes in
Colorado. When you start the final descent into Telluride, it turns into a
one-way trail, so once you pass that point of no return, it’s do or die
time! I’ve only driven the road to that point and then turned around (didn
’t have good enough tread on my tir... 阅读全帖 |
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h**********g
发帖数: 3962 | 29 G. Semerjian, L. F. Cugliandolo, and A. Montanari,
On the stochastic dynamics of disordered spin models,
Journal of Statistical Physics, 2004
A. Montanari and M. Mezard,
Hairpin formation and elongation of biomolecules,
Physical Review Letters, 2001
Y. Kanoria, M. Bayati, C. Borgs, J. Chayes, A. Montanari,
Fast Convergence of Natural Bargaining Dynamics in Exchange Networks,
SODA 2010
注解：SODA 是个算法理论会议，排在STOC和FOCS之后。
Y. Lu, A. Montanari, B. Prabhakar, S. Dharmapurikar, and A. Kabbani,
Counter Bra... 阅读全帖 |
|
q**x
发帖数: 1636 | 30 I am a consultant in Houston, TX; Oil and Gas companies retain our firm to
find qualified candidates for their open positions. I wanted to see if you
or anyone you know may be interested in a Heat Transfer Engineer role with
one of my clients?
My client is a leading international supplier and licensor of process
technology, catalysts, adsorbents, process plants, and consulting services
to the petroleum refining, petrochemical and gas processing industries.
Attached you will find the role specifi... 阅读全帖 |
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q**x
发帖数: 1636 | 31 【 以下文字转载自 JobHunting 讨论区 】
发信人: quix (uglyDuck), 信区: JobHunting
标 题: Opportunity/Referral - Heat Transfer Engineer
发信站: BBS 未名空间站 (Mon Aug 26 15:39:06 2013, 美东)
I am a consultant in Houston, TX; Oil and Gas companies retain our firm to
find qualified candidates for their open positions. I wanted to see if you
or anyone you know may be interested in a Heat Transfer Engineer role with
one of my clients?
My client is a leading international supplier and licensor of process
technology, catalysts, ... 阅读全帖 |
|
m*****g
发帖数: 1717 | 32 爬秃头山.周六上午9点trailhead集中.那里基本没有手机信号,所以不要迟到,过时不侯.
Trailhead: Take the 210 to Upland and exit Mountain Avenue heading North into the Angeles National Forest. Follow Mountain Avenue to the intersection with Shinn Road - veer left. Continue to Mt. Baldy Road, veer right onto Mt. Baldy Road and follow it for a few miles to Manker Flats. Be careful of your speed after Baldy Village - some of the turns are hairpin.
Turn left at the sign for San Antonio Falls Road. Park here, but don't block the gate, peo |
|
v***y
发帖数: 7583 | 33 ☆─────────────────────────────────────☆
motncsg (只拍糖水片) 于 (Mon Dec 10 11:11:34 2007) 提到:
爬秃头山.周六上午9点trailhead集中.那里基本没有手机信号,所以不要迟到,过时不侯.
Trailhead: Take the 210 to Upland and exit Mountain Avenue heading North into the Angeles National Forest. Follow Mountain Avenue to the intersection with Shinn Road - veer left. Continue to Mt. Baldy Road, veer right onto Mt. Baldy Road and follow i
a few miles to Manker Flats. Be careful of your speed after Baldy Village - some of the turns are hairpin.
T |
|
f**********o
发帖数: 793 | 34 大家喜闻乐见的Grififth Park, 闹市中的绿地。不到5mile, 很轻松的风景之行。
请同好 1:00 pM碰头 @Gate. GPS 可以用这个地址协助：3400 North Beachwood
Drive， Hollywood, CA 90068。实际上过了Gate就要找路边的parking. 还要走一段
dirt
Rd 才会到Ranch.
http://maps.google.com/maps/ms?
ie=UTF&msa=0&msid=116846771295287559275.000482bc04834f1ec4e7b
运动后可以去腐败,欢迎推荐餐厅。
请大家做好防晒，带上帽子和足够的水。
电话: 310-751-0797
Mt. Lee and the Hollywood Sign
This hike provides you with a hefty dose of instant gratification.
After going west on Franklin Avenue and turning right on Beachwood
Drive,... 阅读全帖 |
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s******g
发帖数: 2318 | 37 前天听NPR里讲到晚清末年一个统治中国43年的人，一想那不就是慈禧老佛爷嘛，果然
后面播音员发音cixi还是蛮准确地...从里面学了几个词 dowager，concubine...都说
兼听则明、
偏信则暗，咱看看老外眼里的慈禧太后，hehe
link：
http://www.npr.org/2011/12/19/143796431/powerful-portraits-capt
Freer Gallery of Art and Arthur M. Sackler Gallery, SC-GR 249
Empress Dowager Cixi poses with the wives of foreign envoys in Leshoutang,
the summer palace in Beijing, circa 1903-04.
text size A A A December 19, 2011 Intrigue! Riches! Sex! Some violence! Not
the latest movie plot, but a story that lurks in t... 阅读全帖 |
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s**a
发帖数: 1995 | 38 《紐約生活》12月9日~12月15日
本報訊
December 08, 2013 06:05 AM | 512 次 | 0 0 評論 | 2 2 推薦 | 電郵給朋友 | 打印
聖誕故事音樂劇
聖誕故事音樂劇
給世界的49首圓舞曲
給世界的49首圓舞曲
聖路加樂團
聖路加樂團
P!nk﹕愛情的真相
P!nk﹕愛情的真相
龜島四重奏
龜島四重奏
【12月9日 星期一】
●龜島四重奏與蘇頓演唱會Turtle Island Quartet
時間﹕1pm
地點﹕布魯克菲爾德廣場(Brookfield Place)，曼哈坦下城西街(West St.)100號交維
西街(Vesey St.)
查詢﹕http://brookfieldplaceny.com，(212)417-7000
門票﹕免費。在這個被格萊美(Grammy)獲獎樂團「龜島四重奏」的音韻繞樑的環境下，
好好享受一個周一下午吧。「龜島四重奏」將與獲格萊美提名的爵士樂歌手蒂爾尼
蘇頓(Tierney Sutton)攜手，向大家送上跨越文化的表演，迎接冬至。這次表演以傳統
歌曲為主，從猶太教光明節音調、16世紀英國頌歌，到印度教排燈節樂章... 阅读全帖 |
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f*********8
发帖数: 583 | 39 We play men double. One of the guy is fast to save net hairpin, net drop and
net push, block. His backhand is usualy bouncing to the back tram line
defensive clear. He can save smash with net drop or drive crosscourt. His
only weakness is backhand defense against smash as a lefthander.
What kind of strategy to play this guy, when he partner with a defensive guy
who will return any shot in the net high, four corners. |
|
f*********8
发帖数: 583 | 40 We play men double. One of the guy is fast to save net hairpin, net drop and
net push, block. His backhand is usualy bouncing to the back tram line
defensive clear. He can save smash with net drop or drive crosscourt. His
only weakness is backhand defense against smash as a lefthander.
What kind of strategy to play this guy, when he partner with a defensive guy
who will return any shot in the net high, four corners. |
|
x***8
发帖数: 369 | 41 裁判判的是invading the opponent’s court，不是Obstructions。这个时候
obstruction只能是小李。
13.4.2
invades an opponent’s court over the net with racket or person except that
the striker may follow the shuttle over the net with the racket in the
course of a stroke after the initial point of contact with the shuttle is on
the striker’s side of the net;
谌龙扑球，李宗伟是放弃的状态，手上没动作，谌龙怎么影响李宗伟？李宗伟是想搓个
Hairpin net shot, 让高点在自己这边，球在下降的时候过网，从而限制谌龙进攻。谌
龙这个不是brush net kill（击球前或击球时拍子部分已经过网，但击球点在己方场地
），所以如果你同意击球点在谌龙一方就说明问题了。
13.4.4
o... 阅读全帖 |
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y**b
发帖数: 10166 | 42 hairpin这说法第一次听到，U型搓球，有趣，感觉至少是一种很重要的搓法，盖德早年
特别喜欢搞这种搓法。
咋没人说锅铲搓球法呢，还可以区别凹锅铲搓球法、平锅铲搓球法等等，呵呵。这倒不
是凭空胡编，我记得很久以前大家练习搓球时，就有人说，这不是炒菜吗？大乐。
to |
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b******n
发帖数: 1269 | 43 Oceanside roads in FL, straight, wide, sunny;
Suburban rolling hills with gentle climbs (NY, NC, etc.);
Manhattan, fighting with cabs, alongside messengers;
Mountain passes in the Alps, brutal climbs (there was one I almost cried
when I saw it), 55mph descents, hairpin bends, etc.
Crusing through Italian villages, cobble stones, narrow roads, waving to
coffee shop owners, etc.
Which do you like most? |
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w********6
发帖数: 12977 | 44 Mountain passes in the Alps, brutal climbs (there was one I almost cried
when I saw it), 55mph descents, hairpin bends, etc. |
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h*****a
发帖数: 1992 | 45 hehe, at the hairpin of 84.
Envy you!
commute。 |
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o*****e
发帖数: 379 | 46 看着天气预报一天比一天悲观，都以为又要cancel了，星期三晚上，连着预报的雨天里
突然冒出来一个可能的晴天，星期四Wes让我们投票，大家都愿意去碰碰运气，所以周
四晚上照常出发。结果天气比我们想象的好多了，基本没什么云，视野很开阔。晚上在
Hairpin turnover的碎石地上camping，满天的繁星和大片大片的星云，看得好清楚。
星期五早上6点半从停车场出发，翻过一个很矮的boudler的小土坡，到达pass下面的那
个boulder大坡之后，直接往左走，在 boulder坡的左缘有trail。往左、往上，trail
很快消失在另一片boulder区里，这里到pass，boulder和trail各一半，late season一
般也就这condition了。一个小时左右上到pass，沿着比较solid的石头drop很缓的一段
，转过弯去看见右手侧最突出的那个尖顶就是Kangaroo temple。这一段有很多很大的
、光滑的slab石头，别上去，尽量保持在它们下面贴着edge走。最后一段approach没有
trail，就是自己找路朝着climb base的方向走，一路全是碎 |
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c*******r
发帖数: 13580 | 47 那个course超过了5k，很多人都这么反映，我觉得差了大半分钟。而且很多hairpin
turn，上下坡也多，还有楼梯，人又多，至少要减掉2分钟才是真实水平 |
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c*******r
发帖数: 13580 | 48 There should be plenty on this board. I have never raced a flat and straight
5K course but my guess is I'm not too far, no more than 20 seconds. My last
5K race was 20:40 but it had some ups and downs, a few 90 degree turns and
a hairpin turn. |
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m****z
发帖数: 978 | 49 Follow these tips to avoid swimmer's ear:
By Mayo Clinic Staff
Keep your ears dry. Dry your ears thoroughly after exposure to moisture from
swimming or bathing. Dry only your outer ear, wiping it slowly and gently
with a soft towel or cloth. Tip your head to the side to help water drain
from your ear canal. You can dry your ears with a blow dryer if you put it
on the lowest setting and hold it at least a foot (about 0.3 meters) away
from the ear.
At-home preventive treatment. If you know you don... 阅读全帖 |
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h******n
发帖数: 734 | 50 they are not much different. rad has lighter hairpin gives u room to add
silicone, rad hoop is heavier and softer
Radical |
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