A******y
发帖数: 2041 | 1 Where do you get your HDACs? Also, which HDACs? The in vitro data for most
class IIa HDAC are wrong...including HDAC8. Class IIa and HDAC8 do not
deacetylate the acetylated substrate (or very little)...they do bind though.
I can give you an activity list...
Class I (Ac substrate)
HDAC3 > HDAC2 > HDAC1
Class IIa (TFA substrate)
HDAC8 > HDAC4 = HDAC5 = HDAC7 = HDAC9...
Class IIb (Ac substrate)
HDAC6 not so active but better than HDAC1
HDAC3 is at least x100 time more active than HDAC1/2 in vitr... 阅读全帖 |
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d*****s
发帖数: 647 | 2 Nature. 2013 Nov 7;503(7474):146. doi: 10.1038/nature12727.
Retraction: Functional dissection of lysine deacetylases reveals that HDAC1
and p300 regulate AMPK.
Yu-yi Lin, Samara Kiihl, Yasir Suhail, Shang-Yun Liu, Yi-hsuan Chou, Zheng
Kuang, Jin-ying Lu, Chin Ni Khor, Chi-Long Lin, Joel S. Bader, Rafael
Irizarry & Jef D. Boeke
Nature 482, 251'255 (2012); doi:10.1038/nature10804
In response to a concern raised by a reader about inconsistencies in our
Letter between the results from the primary mi... 阅读全帖 |
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J********3
发帖数: 3151 | 3 是，还有YAF2，YY1等。HDAC1, HDAC2，E2F6也常被PRC1或PRC2拽下来，KO也有Polycomb的表型，但不知为啥不把他们算做Polycomb，Cbx7有人认为是Polycomb，但原来一直做Polycomb的人反对。 |
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l**********1
发帖数: 5204 | 5 Alleviating interactions.
Please go to AMPK(Sip2) Nature paper:
Functional dissection of lysine deacetylases reveals that HDAC1 and p300
regulate AMPK (2012)
link:
//www.ncbi.nlm.nih.gov/pubmed/22318606
its pp251
called both as
[synthetic lethal (negative/aggravating) and rescue (positive/alleviating)
interactions]
more reference:
Acetylation of yeast AMPK controls intrinsic aging independently of caloric
Cell 146: 969-
//www.ncbi.nlm.nih.gov/pubmed/21906795 |
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l**********1
发帖数: 5204 | 6 sunnday 个把月前问 酵母内的 协同致死的叫synthetic lethal factors
而协同延命的叫啥
matrix 无意中搜到一篇nature
Lin YY et al. (2012)
Functional dissection of lysine deacetylases reveals that HDAC1 and p300
regulate AMPK.
Nature. 482: 251-5.
//www.ncbi.nlm.nih.gov/pubmed/22318606
就回帖她/他
可能叫
synthetic rescue factors
楼主你的原开贴 还有 antagonistic rescue factors 的可能性
当然 antagonistic lethal factors 的话 达尔文演化论中的不适应者 可能就无后了
具体如何定位pathway 0.44 概率以上的经典规则 不一定适用
-----just matrix two cents |
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d*********w
发帖数: 6 | 7 这个Lin YY 很高产啊，三年内三篇CNS。高度怀疑其他paper的可信度。。。那个Lu JY
好像是他老婆。
Functional dissection of lysine deacetylases reveals that HDAC1 and p300
regulate AMPK.
Lin YY, Kiihl S, Suhail Y, Liu SY, Chou YH, Kuang Z, Lu JY, Khor CN, Lin CL,
Bader JS, Irizarry R, Boeke JD.
Nature. 2012 Feb 8;482(7384):251-5. doi: 10.1038/nature10804.
Acetylation of yeast AMPK controls intrinsic aging independently of caloric
restriction.
Lu JY, Lin YY, Sheu JC, Wu JT, Lee FJ, Chen Y, Lin MI, Chiang FT, Tai TY,
Berger SL, Zhao Y, Ts... 阅读全帖 |
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A******y
发帖数: 2041 | 8 It really doesn't matter if it is chemical modification or biological
modification. Some scientist in the field believe a lot of acetylation
modification is through direct chemical transfer of Acetyl-CoA without the
help of HAT espeically in organelle enviroment favoring nucleophilic
addition.
As far as crotonyl-modification. My friesd published in a good journal
stating HDAC3 is the de-crotonylase...my lab results are exactly opposite.
HDAC1 and HDAC2 have more activities against cortonyl-ly... 阅读全帖 |
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X******n
发帖数: 914 | 9 All the authors have signed this retraction with the exception of Y.-Y.L.,
who is deceased. |
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g*********3
发帖数: 177 | 11 这篇文章。。。。也能retract啊。。。LZ能否给出链接，google了下没找到出处。谢
谢。 |
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p*****c
发帖数: 20445 | 12 这篇文章背后的故事很多。。。好像美陆台都牵涉到了？ |
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H****N
发帖数: 997 | 15 This makes one wonder how many more papers in CNS are actually junk but will
never be found out. |
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l**********1
发帖数: 5204 | 16 wet bio fields if WB figure used high contaction those CNS also N sister if
IF higher than
20.0 might be >1/3
there are fasle result/s. >1/10 could be retracted within one or two decade.
..
will |
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A******y
发帖数: 2041 | 18 That's right, it turn out high impact paper in lower IF jounrals have best
reproducibility. If I remember correctly is above 70% by someone completely
independent.
if
decade. |
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s*********y
发帖数: 292 | 19 who is deceased....
HDAC1 |
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A******y
发帖数: 2041 | 21 Just mention how Schreiber cloned HDAC1 before any biologist. However, a
biologist and a chemist made and translated the first HDAC inhibitor without
knowing the target at the time. |
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