|
发帖数: 1 | 2 做ChIA-PET的多的是啊,pubmed一搜一大把。
[在 zxt (Asoka & Akaba) 的大作中提到:]
:You mean Erez's lab? |
|
J********7 发帖数: 43 | 3 Sorry. I am a little out-of-date.
I mean Yijun Ruan's lab. |
|
y*********1 发帖数: 46 | 4 The resolution is mostly dependent on sequencing depth. In Erez's Science
paper, the resolution is 1 Mb from 8 million reads. In his Cell paper, the
resolutions are 1-5 kb from 3 billion reads.
Just Google Chia pet. |
|
e*********6 发帖数: 3453 | 5 不是啥大钱啦,在工业随便做点东西几万几万的
It |
|
p*****e 发帖数: 989 | 6 如果是Wei chia lin 实验室的那和阮是一个,不算 |
|
z*t 发帖数: 863 | 7 最近Rick Young实验室有一片science也发了ChIA-PET
:如果是Wei chia lin 实验室的那和阮是一个,不算
:☆ 发自 iPhone 买买提 1.22.06 |
|
|
发帖数: 1 | 9 理论性的学科被推翻很正常,实验性的像韩春雨的被推翻就是被证明造假,不能自欺欺
人。看你能上买买提智商应该还算正常,不应该不明白这个道理啊 |
|
|
|
D*a 发帖数: 6830 | 12 这不是中医粉和上帝粉的理论吗?
“很多科学理论后来都被推翻了,你现在说的也不一定对,你怎么能保证以后没人懂中
医/上帝/跳大神?” |
|
l********e 发帖数: 415 | 13 Genome wide epigenetics 基本是扯。最明显的就是enhancer的histone mark, 靠这个
预测出来的enhancer至少有一半是无法验证的。至于3C 4C hiC, 就基本是胡扯了。 |
|
发帖数: 1 | 14 很对研究都是描述性的,比如profiling of epigenetic landscape of XXX , HiC
map of XXX这种。
大家怎么看这类研究?
Descriptive research does not answer questions about how/when/why the
characteristics occurred.
Hence, descriptive research cannot describe what caused a situation. Thus,
descriptive research cannot be used to as the basis of a causal relationship
, where one variable affects another. In other words, descriptive research
can be said to have a low requirement for internal validity.
The description is used for fr... 阅读全帖 |
|
m********m 发帖数: 11 | 15 一个基因的话还是做4c吧比较便宜,虽然4c也不好做但是比ChIA-PET还是容易多了。不
过4C也只是半定量,如果差别不是特别显著的那种也很难观测到。
做HiC的话不光贵而且分析也是个大坑,现在几个大组的数据分析标准都不统一,一个
人从头理太费时间了,希望4DN能建立起行业标准。 |
|
m********m 发帖数: 11 | 16 至少有两个组都做了老鼠早期胚胎发育里面的HiC了,都已经投出去了,现在再做海胆
的是不是有点不够新了。。。 |
|
c*********r 发帖数: 1312 | 17 细胞周期中的HiC有人做过没?比如一个细胞周期中每隔一段时间取样什么的。 |
|
d********m 发帖数: 3662 | 18 Random matrices在这么下面?hic的精华啊。
我对Erez刮目相看了。面试的时候我说这东西我现在不懂,在你那里开始之前我尽量学
会,现在我觉得他的谜之微笑应该是藐视之微笑了。真是无知者无畏啊。这么难,幸好
没去,不然估计几年后该学不会还是学不会。 |
|
发帖数: 1 | 19 本来发paper是为了学术交流,现在却都要追求大而全。最近看了一篇paper,是重大技
术进展,完全没有贬低贡献的意思
https://www.ncbi.nlm.nih.gov/pubmed/25497547
他们采用了一种新技术,新流程,可以让HiC分辨率提高。本来,讲完技术,流程,确
认了新流程的数据是正确的,这就行了。本来,到了这步,这就应该已经能奠定地位了
,以后,想用技术的可以用,想分析数据的直接下载,这的确是重大贡献。但是。。。
。。。后边好几节都是讲新数据带来了什么发现,让文章成为审稿人喜欢的一个完整故
事。这本来没错,但是,这让必然让文章发表时间拖后,本来这段时间,学术界应该都
拿到数据进行分析了,但现在,这段时间只有他们组内部有,使整个技术的普及晚了至
少几个月。
这好比说,某人发明了一辆更省油的车,讲完技术,证明了真的更省油,这就可以了,
是很大的贡献,但是要满足审稿人的要求,还要写,如果用这车当出租车,可以给司机
省多少钱,如果用来当私家车,可以少排放多少温室气体,感觉完全是画蛇添足。 |
|
x****6 发帖数: 4339 | 20 看来是研究基因组空间结构的同好,
请问HiC这种技术吧三级结构整出来以后,除了炫酷以外,还有什么用?
nuclei
coordinations
we
real |
|
x****6 发帖数: 4339 | 21 HiC 的重复性怎么样?
同一种细胞,在相同条件下,今天做和隔一年做,得出的map是一样的吗?
了。 |
|
d***e 发帖数: 1215 | 22 my suggestion:
CN or C4 column
column temperature >60C
Reduce mAb if possible
or
Collect peaks from HIC column
or
SEC, if just need desalt mAb for MS |
|
yf 发帖数: 272 | 23 看到你的情况,感到很难过与震惊。我猜想你女儿是不是有这个病: myocardial
bridge.
下面有些文章你可以看:http://www.texasheart.org/HIC/Topics/Cond/MyocardialBridge.cfm
这是一种先天心脏病。我见过一些病人有这种毛病。但是还没有看到有人死于这个病。
这种病是这样的:正常人的冠状动脉是走在心脏的表面,但是的这种病的人,他们的冠
状动脉被心脏肌肉盖在上面,也就是说他们的冠状动脉有一部分穿过了心肌。所以当心
肌剧烈收缩的时候,会挤压冠状动脉。而冠状动脉是供应心脏血供的,被挤压就会引起
心肌缺血。心肌缺血时间一长,心肌就会坏死,就是心肌梗塞。大多数的myocardial
bridge的病人都没有症状,严重一点的人有心绞痛,更严重的会有心肌梗塞。我猜想你
的女儿摔下来以后太过惊恐,心脏剧烈收缩,挤压了冠状动脉,引起心肌梗塞而去世。
具体情况我不知道。上面也都是根据你所描述的尸体检查报告的猜想。
我觉得看孩子的地方应该有责任。但是你是否告得赢我不知道。你可以咨询心脏科的医
生,还有律师。至于重新尸体解剖,我不知道隔了这么长... 阅读全帖 |
|
k****r 发帖数: 593 | 24 本人估计还有一年毕业,化工专业,主要是抗体蛋白质提纯方向。
•Specialized in protein purification process including chromatography
(AC, IEC, SEC, HIC), filtration (TFF, UF, DF, NF), precipitation, etc.
•Specialized in protein quantification including ELISA, SDS-PAGE,
Western blot, densitometry analysis, BCA, Bradford, enzymatic activity
analysis, UV-Vis.
•Specialized in aseptic techniques, media constituents and media
formulation strategies, microbial and animal cell culture.
•Hands-on experience on pr... 阅读全帖 |
|
g*****1 发帖数: 666 | 25 听说许多药厂试图绕过proteinA 只用IEX或HIC for manufacturing.一次几千升,甚至
上万升的发酵液要用proteinA,成本也是巨大的。当然preclinical,phI,phII的生产
应该还是多数用proteinA。
也想了解这方面的进展。 |
|
s******e 发帖数: 1073 | 26 只用IEX或HIC for purification, 能快速纯化吗?
已经在用这种方法了还是正在考虑阶段 |
|
f****6 发帖数: 4472 | 27 同求推荐啊,fresh Phd, DMPK方向,gut microflora metabolism, small molecule
assay development, protein purification (IEX, HIC, GFC, SDS-PAGE etc) and
quantification (LC/MS) |
|
y******g 发帖数: 317 | 28 所以才有了hic, hippa, irb etc.....
有些 |
|
c**r 发帖数: 10001 | 29 这是啥啊?hic是human investigation committee? 有空科普一下吧?:) |
|
m****s 发帖数: 18160 | 30 ☆─────────────────────────────────────☆
mitbbs (未名空间) 于 (Fri Jan 20 20:13:05 2012, 美东) 提到:
各位网友,
mitbbs 全体工作人员给大家拜年了!中国新年-龙年即将来到,mitbbs全体工作人员祝
大家新年快乐,新年家庭美满,工作顺利,事事顺心,财源滚滚来...
mitbbs 会按国家规定,放年假,与家人欢聚,与朋友叙旧。
故放假期间,有一些事务会推迟到年后进行处理,请大家理解。
谢谢您们一直一来对未名空间的支持与厚爱。
(PS:请大家回复此帖,并写下新年祝福语,留言的网友mitbbs都会发放 龙年拜年饺
子,年后一并发放)
mitbbs 站务组
☆─────────────────────────────────────☆
DUV (毒物) 于 (Fri Jan 20 20:23:43 2012, 美东) 提到:
祝福站务
祝福自己
龙年更上一层楼!
☆─────────────────────────────────────☆
BUV (yaz无耻,欠债不还) 于 ... 阅读全帖 |
|
m****s 发帖数: 18160 | 31 成功奖励 10 伪币的用户: DUV, BUV, EUV, wxx033000, Unstoppable, iForpus,
blueforpus, xiangmoni, bamboo2000, tempuser, peipei2008, Joyapple, caoyu,
pepsifan2, bigbread, kqsm, goldmetal, kaylaxu, alovely, Ametc9, yym, faniu,
Grad, LOV, moneybull, sioc, ladyred, xhzhou, donthurtme, wetpoet, miluo,
mihu, tellmenot, minibread, mmengg, Orcus, daolang, chrisfly, Ann, zhijier,
meiguohuaren, lisafly, softknife, donttell, cressida, Rosmarinus, FIBA,
alliah, woa, hateorlove, meflying, netll, faner, lxinzheng, Fundin... 阅读全帖 |
|
m****s 发帖数: 18160 | 32 ☆─────────────────────────────────────☆
mitbbs (未名空间) 于 (Mon Jun 4 00:04:32 2012, 美东) 提到:
区段删除、普通删除等版务功能已经恢复正常,版主可进行正常版务操作。
近期站上出现异常,由此给大家带来诸多不便,实在抱歉。
因此,在2012年6月4日00:00:00-6月5日00:00:00期间,于此帖下跟帖回复,将有一个
包子略表歉意。
感谢大家长期以来对mitbbs的理解和支持,祝站上所有朋友生活愉快!
Mitbbs站务组
2012.06.03
☆─────────────────────────────────────☆
kx (老k) 于 (Mon Jun 4 00:07:27 2012, 美东) 提到:
re
正急着清水呐
☆─────────────────────────────────────☆
MrYi (小易) 于 (Mon Jun 4 00:11:42 2012, 美东) 提到:
好
☆────────────────────────────────... 阅读全帖 |
|
m*********k 发帖数: 10521 | 33 [Fishing] pplulu Jun 14 ● [bssd]啥也不说了,发包子250个,前50个双黄
20*50+10*200=3000
成功奖励 20 伪币的用户: techsavvy, DrDonkey, lyrock, massfish, Strike,
topten, offernow, heavyduty, fishing66, moja, eggonlyegg, kaolan, realbasten
, submar, stratifin, dream888, kendal, goubeibei, happybuda, duoduoduo,
zillionaire, tndb, weston2000, cutecherry, Belichick, JianlianYi, chonglou,
bamboo2008, lingyunxie, maxelement, tranquiIness, pmkcug, Mutombo, Master,
alovely, yasha, hic, Spark, Funding, Baishunlai, ZBo, isis1... 阅读全帖 |
|
m*********k 发帖数: 10521 | 34 [NewYork] kingcw Aug 23. ● 版上发一轮包子给大家压惊+地震需知
成功奖励 10 伪币的用户: liulinglll, majia55, YaTou22, zenoat, zaqizaba,
iamapig, Vesper8, Belichick, ych99, bubua, sillusorydl, LXJSmonk, lsxh,
webnet, tinachen1984, sunnybunny, yve, mxf, ThisNoName, Glamor, mapleleaf16,
yyll51, baoshu, yansandy, guaichong, stinkbug, shua, Mutombo, ylonglew,
GreenVol, aniee, lyufeng, mtwzy, lxinzhen9, soyjoy, liuzhu, scoreboard,
paotangde, atu2010, ytf, evenhorizon, kicku, yaya09, yumeiren918, lamprey,
IceStick, kelvinc... 阅读全帖 |
|
m*********k 发帖数: 10521 | 35 [Girl]
55211 onearcat Aug 28 ● 等飓风过去了 偶要是还活着
成功奖励 10 伪币的用户: superguava, Iamhonest, UNO, opamp741, lxinzheng,
falling, chongchong1, wxn03, ADOMET8, scc, walkingtall, hic, MissA, dc1555,
huoduoduo, abcxyz2046, carlx, xqingxl
扣除版面:(Girl)180伪币成功 |
|
m*********k 发帖数: 10521 | 36 [Collectibles]
60646 honde Sep 11. ● 喜迎中秋,版面发99个月饼包回馈版众
成功奖励 10 伪币的用户: daj, hua717, EAdreamer, NeoEgg, fbfb, jameswho,
my0606, amo, laomonkey, socall, philistin, Belichick, hoto1668, bigjohn,
Dwayna, reign1983, Vesper8, Seanna, crazyman, foolsgarden8, wellmoon, wtfbbq
, AnyMoreLeft, Renshaw, didarsmile, oceanside, meilefu, allen04, goldcoin,
majia666, ADOMET8, santorini, lsf, Jingqg1979, BrainBow, measles, Rosmarinus
, delicacy, II1, quadpod, KAROUN, cjiayun, bia, LittleSeven, wxn03, SQ... 阅读全帖 |
|
m*********k 发帖数: 10521 | 37 接上页:
AJ0000000000, smart1118, ADOMET, LittleSeven, shidatou, PSUbso, wqdv,
louadams, SpicyGirl, clematis, miaomiao87, ceaj, Sickdeal, lxinzheng, gofar,
chentia, Topeka, dbr, happigee, lingxiaoyan, rongbinh, raei, taotao701,
daxue, cocomin, RazorEdge, smthiid, dbnf, aj000000000, xixi1101, lctl,
rungriffin, chaselife, JN, ywcai2005, fajn, integrin, dahuani, II1, bjbro,
hte, hab, GIJOE, mosessu, lbyo, springtai, maplezh45, PageA, liondream,
westview2011, summitzf, blueclassic, AllenPF, Beisner, jay... 阅读全帖 |
|
m*********k 发帖数: 10521 | 38 [ebiz] cressida Sep 24 ● 感谢师傅
成功扣除 1100 伪币(含10%手续费)的用户: cressida
成功奖励 10 伪币的用户: Ada2008, liziiiiii, ZQL, Tina0929, diamondking,
samurairen, canyu, JL2011, feyisgod, boomking, happylux, II1, chrisfly,
lisafly, laoer, Potatokiller, LTA, louadams, xueyou38, Master, bactive79,
taotao701, xiaoguapi, pppiggy, aitian, party, koalabears, RWR, MissJ, bbdou,
Adriano, ImHot, bigbread, Funding, Itis2010, watercrane, monmon, ImOK,
zhuid, PandaSyr, luyelina, sunzhq04, eggpain, ihouse, IC, tranquilness,
... 阅读全帖 |
|
m*********k 发帖数: 10521 | 39 接上页:alsin, Mobot, measles, monosky, Carrpool, Sickdeal, blueforpus,
hskevin, chouwacuipin, xxchijia, yingzi198210, GGlovesBB, tonyparker, qmf,
geminitwo, server, iForpus, blossoms, myloveli, Unstoppable, beperfect, win7
, feimo, pichu, zhouyu1016, allin888, diamondking, littledevil, JN,
GreenRiver, BooBair, kakapo, Nacho2000, yunbao, lucky199, MrYi, KANKAN1225,
rackyfx, YRH, kelly9988, cyyxx76, ll111, maplezh45, xinting457, kanlezi,
happylux, onetwoome, coldplayer, summer, llttjr, luyelina, xian... 阅读全帖 |
|
m*********k 发帖数: 10521 | 40 接上页:sunzhq04, shren, lynn2007, MissA, AJ0000000000, zzfq, kokosky,
ADOMET8, jokeyzhu, liuge, mgbm, kitty07, snowmit, PSUbso, gaospring,
isisl414, man8, letmethink, robck, ganoderma, happyblue, Mj12, shenma, vivea
, DHA, gofar, jghnr, happybabies, aniee, aj000000000, iouczs, coolbeans,
crystal87, clematis, crystalapple, save12345, chouwacuipin, tan2010,
dieyipiao, variance, lailai11, alli, dusky, AJ00000000, kotex, angelica, hys
, goskyline, Tally, kingcat, VichyBoy, shabo, fensiqueen, polymacro,... 阅读全帖 |
|
m*********k 发帖数: 10521 | 41 接上页:Rodimus, cnnssb, markh, umner, zzz59, FlyingBrothe, bigbaozi,
harding, atu2010
, Febaugust, goddy, matthewh, bbcool, BeckyHO, IC, oldgordon, cm123,
scoreboard
, Simin, hynek, realman888, lbanzheng, kevinj, vanddany, wobest, wsx123,
Unstoppable, smartkevin, xiaodaigua, astra, yansohu, Biofuel, IPLAW, ovoneL
, almeda, candy1980, dongge, beetle1986, Acartia, graceWA, mttbbs, reign1983
, BRavens, EC2, Skylla, KAROUN, gavin1972, DarkHill, lyufeng, colour, samiya
, erjie, hhaini, pinkplumeria, Mmj... 阅读全帖 |
|
m*********k 发帖数: 10521 | 42 [Immigration]
85503 ladyred Oct 16 ● 感谢新老朋友对本届版务工作的支持,发包子大概
前200个回帖ID奖励:成功奖励 10 伪币的用户: vonstone, city, Renshaw, yansandy
, aplets, caomuzhiren, ssippian, fbfb, zhlltu, PQOI, iSeven, chouwacuipin,
lailai11, magiclinux, lucifer11, mihoutaoboy, whaha, sticktoit, kotex, Songp
, fairway, gr, broodtonight, shabo, jjl231, VividMay, famulus, gcvv,
kathylee, TrueStory, Rosmarinus, caoyuan, macromol, ADOMET8, wrxys,
OrangeJuice, yearly, victorye, shawjone, pictureID, Unstoppable, clematis,
binbinz... 阅读全帖 |
|
m*********k 发帖数: 10521 | 43 [Living]
89745 Watergirl Jan 20 ● 过节放点粮,房奴们也可以吃点大白
面了
成功奖励 10 伪币的用户: enonieerht, Han7788, Huan2007, citymouse,
hiwaycruiser, papertiger, astro, wakao, TodayEscape, bed, yourway, sisomso,
dingdingcat, gregory, youwenbida, starboard, UniversalT, jumpharder, ThinkU,
fionana, lxingbo, lilycola, Ilikesummer, Resell, zhongdianshi, skyandflying
, sunnysocal, ffkiller, yaotouwan, NewsEgg, airjojo, Bullby, Teamster,
shelter, apps, changduyang, plus, hjxds, orang, yesshell, HeGuaMei, tPQ,
shinma, th... 阅读全帖 |
|
m*********k 发帖数: 10521 | 44 "[Geisha ]Geisha Feb 2 ● [bssd]谢谢各位支持, Geisha顺利当选十大优秀
版"
成功扣除 3300 伪币的用户: Geisha
成功奖励 10 伪币的用户: ZQL, ingoodmood, mypeak, XOXO2012, oriolechao01,
phox22919, jiawen, leooooo, dzhlh, happigee, PInce, hunzaimeiguo, omo, kkbb,
Go8, Orange23, Haoyun2011, tndb, haaaaaappier, MissA, fisherduyu, navaratna
, lxinzheng, hskevin, rainyj, hic, yiban, aplets, ruskiller, Buxinjiao,
Quill, comcom, ludeackpower, qw, yasaka, donic, stiga, goldmetal, DHA,
aablackk, ivylee16, juic, tibhar, Joola, Acousticia... 阅读全帖 |
|
m*********k 发帖数: 10521 | 45 1.
"[bigchipmunk]bigchipmunk Feb 2 ● [bssd]龙年糖葫芦元宵汤圆(whatever...
)200串"
成功扣除 1804 伪币的用户: bigchipmunk
成功奖励 10 伪币的用户: LisaHayes, lezizi, wells, goldy, ADOMET8, bos,
aiyayayaya, lhr, wanwanmeimei, LeChuck, hualavender, iSeven, am1, megguan,
chrr, aae, lxinzheng, NWWolf, hpbd018, NWWoIf, SETiger, lilybank, goose,
YuanShihKai, chatter, chex, churchance, xi1, rub, yiban, winter, ebw,
futurehw, Mine, Kua, dramawatcher, wzxm, masara, Google008, laxger, aerozxy,
ASLA, maywind, onion2010, TimeToMove, i... 阅读全帖 |
|
m*********k 发帖数: 10521 | 46 qiaqiafeng Feb 14 ● [bssd]恭喜板斧们上岗,100个签证包
扣除版面:(Reunion)1000伪币成功
成功奖励 10 伪币的用户: buffalochick, scorpic, server, pdd, lucifer11,
cerealmilk, watercrane, yaokarl, win7, firsfirs, bactive79, aifanfan,
tempuser, win8, harrietann, monmon, weiyu1, LCK, mmengg, yuli1001, AGP,
akoug, beibeimiao, syq2009, TRex, jokeyzhu, KDnewborn, datoufei, skyhorse,
equipmentl00, xuantai, yysqq, wedidit, meng09, oriolechao01, pepsifan2,
leooooo, smurf50, wwtwins, catdoudou, xlucy2008, haaaaaappier, kqsm,
peterzhao, b... 阅读全帖 |
|
m*********k 发帖数: 10521 | 47 [Military] 【龙飞凤舞】庆龙年新春联合活动(军事天地、海棠诗社
扣除版面:(Military)10965伪币成功
成功奖励 585 伪币的用户: ladyred
成功奖励 585 伪币的用户: windking
成功奖励 480 伪币的用户: bbppb
成功奖励 480 伪币的用户: waitingforyo
成功奖励 400 伪币的用户: Banlangen
成功奖励 240 伪币的用户: xuxihua, usst1, fishery
成功奖励 160 伪币的用户: CABOY, woodstock001
成功奖励 120 伪币的用户: childheart
成功奖励 80 伪币的用户: dniao, waterer, Unstoppable, JeanIris, abbabb,
cobalt27, iyiya, heather22
成功奖励 40 伪币的用户: choo, n1, SilverBlade
成功奖励 20 伪币的用户: lhr, usst1, wh, windking, woa, aablackk, ACG, ADOMET
, ADOMET8, aiu... 阅读全帖 |
|
t******n 发帖数: 2939 | 48 版面活动无需手续费
活动主题:3月8日国际妇女节活动
标题:女ID请进
原帖链接:不小心删除了- -!
奖励方式:10WB/ID
名单如下:
babymill
Farrell
mmengg
a142857
a18
aac
AadaaPanda
aae
ababa
acidlemon
AdamLambert
ADOMET
adriene
aimer
aip
aitian
aius
alliah
allymcbeal
alovely
alvdena
alyssa03
amber23
Ambiguous
Ametc9
amethystor
amount
amyliu1211
angelakitten
AngelaS
angelaW
angelica
angellove
AnitaB
Ann
anyme
apieceofwhom
aplets
Apollonia
applebb
asia
atlant
August2010
aya1
azmod
b3
b6
b7
BabiRabbit
babying
babykxy
babyworm
BaFei
baixiaoxiong
bambao
bamb... 阅读全帖 |
|
m*********k 发帖数: 10521 | 49 "[WaterWorld]thirdman Mar 7. ● 女ID请进"
扣除版面:(WaterWorld)7590伪币成功
成功奖励 10 伪币的用户: babymill, Farrell, mmengg, a142857, a18, aac,
AadaaPanda, aae, ababa, acidlemon, AdamLambert, ADOMET, adriene, aimer, aip,
aitian, aius, alliah, allymcbeal, alovely, alvdena, alyssa03, amber23,
Ambiguous, Ametc9, amethystor, amount, amyliu1211, angelakitten, AngelaS,
angelaW, angelica, angellove, AnitaB, Ann, anyme, apieceofwhom, aplets,
Apollonia, applebb, asia, atlant, August2010, aya1, azmod, b3, b6, b7,
BabiRa... 阅读全帖 |
|
m*********k 发帖数: 10521 | 50 "[sysop]
mitbbs Jun 3. ● 区段删除等版务功能已恢复正常"
成功奖励 10 伪币的用户: kx, MrYi, moneybull, AGilardino, snatch, lcbp,
Uruguay, fs, joshpang, GoRockets, kvpuh910, soimbox, TSC, cofaky895,
Stoppable, bagua, meiguohuaren, D7, pppttt, Unstoppable, daidai, luckyguy,
GreenRiver, urwumao, AdvancedEdu, wqdv, projective, ll111, lxinzhen,
tismd945, kakapo, BooBair, llttjr, cdpv, raei, shakalaka, flr, reknaz,
churchance, ipeb, ssstockfan, eua, ElliottJr, Kua, muggy, F5, dbnf, lctl,
meoow, ruwumao, jennet, fajn, ra... 阅读全帖 |
|