m*z
发帖数: 2356 | 1 Tom "durrrr" Dwan has started today his big Heads-Up match at Macau, and according to poker room manager of the StarWorld Casino, Stella Yeh, he has been playing against "a guy from Shanghai" ~$4000/$8000 NL Hold'em heads-up.
Few big pots from the game (update from Stella Yeh):
"Hi All,
So as someone has posted earlier, everyone is already aware that durrrr's been playing heads up with a guy from Shanghai for over 12+ hours already. 30k-60k HKD NL . As I've said already I cannot mention who's wi... 阅读全帖 |
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b******1
发帖数: 113 | 2 It has been difficult to see Gidon Kremer in grand symphony halls of big
cities. His new recordings with his Kremarata Baltica Chamber Orchestra have
been exploring new music and redefining what’s in or beyond classical
music. Those titles such as “After Mozart” or “Eight Seasons” sometimes
baffle me: They sound like a young artist’s new installation with
conceptual-wrapped titles; attracting public attention but exposing
uneasiness and lack of confidence at the same time.
But Kremer is no new |
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m***s
发帖数: 1094 | 3
chicks, who were apparently allured by his sexiness.
how is his old sagging body sexy???
he should win a SAG award for that |
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s****t
发帖数: 17096 | 4 because you watch american idol.
Matt is not super on stage, his looks and his style are not great on stage |
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d**w
发帖数: 14889 | 5 NEW YORK – Late-night mainstay Conan O'Brien is headed to TBS to resume his
comedic duties with a talk show expected to debut in November, the network
says.
The program will air Mondays through Thursdays at 11 p.m. Eastern, shifting
TBS' "Lopez Tonight," starring George Lopez, to midnight.
Monday's surprise announcement hit the same day O'Brien starts a two-month,
nationwide comedy tour in Eugene, Ore., amid persistent reports that he was
likely to claim Fox as his new late-night home.
Barring F |
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g****d
发帖数: 3461 | 6 http://en.wikipedia.org/wiki/His_Dark_Materials
Into this wilde Abyss,
The Womb of nature and perhaps her Grave,
Of neither Sea, nor Shore, nor Air, nor Fire,
But all these in their pregnant causes mixt
Confus'dly, and which thus must ever fight,
Unless th' Almighty Maker them ordain
His dark materials to create more Worlds,
Into this wilde Abyss the warie fiend
Stood on the brink of Hell and look'd a while,
Pondering his Voyage; for no narrow frith
He ... 阅读全帖 |
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f*********e
发帖数: 487 | 7 from billboard:
February 15, 2016.
The third time's the charm for Justin Bieber, apparently -- the pop
superstar just won his first Grammy after three nominations for "Where Are U
Now," his 2015 single with Skrillex and Diplo.
youtube video of their winning song MV:
a funny video made by these 3 guys: |
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n******8
发帖数: 558 | 8 Cello and his shadow
A lonely cello,
Talks to no one but his shadow.
In a tone like wavering willow,
In a color like mellowing meadow.
Till the sun flees.
Cello panics and pleas:
Shadow, shadow, stay with me!
For I will sing you nightly glee.
And my strings will never pluck sorrow,
For I need you every tomorrow. |
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发帖数: 1 | 9 有 every tomorrow 的说法吗？
：Cello and his shadow
：A lonely cello,
：Talks to no one but his shadow.
：In a tone like wavering willow,
：In a color like mellowing meadow.
：Till the sun flees.
：Cello panics and pleas:
：Shadow, shadow, stay with me!
：For I will sing you nightly glee.
：And my strings will never pluck sorrow,
：.......... |
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t*******f
发帖数: 2634 | 12 His hair follows his farther:) |
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z*****u
发帖数: 224 | 13 Dear Dad
Berlin is wonderful, people are nice and I really
like it here, but Dad, I am a bit ashamed to arrive
at my college with my pure-gold Ferrari 599GTB
when all my teachers and many fellow students
travel by train.
Your son, Nasser
The next day, Nasser gets a reply to his e-mail
from his dad:
My dear loving son
Twenty million US Dollar has just been transferred
to your account. Please stop embarrassing us.
Go and get yourself a train too.
Love, your Dad |
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F******t
发帖数: 948 | 14 Jamie Foxx Jokes About Killing ‘All the White People’ in His New Movie
Noel Sheppard, Newsbusters, December 10, 2012
Two weeks ago, Oscar-winning actor Jamie Foxx made national headlines when
he called President Obama “Our lord and savior.”
While hosting NBC’s Saturday Night Live this weekend, Foxx joked about how
in his new film “Django Unchained,” “I kill all the white people in the
movie. How great is that?” |
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r*********e
发帖数: 29495 | 15 刚来美时，同住apartment 的是一对中国留学生夫妇，女的是陪读，英文不太好，正在
努力学习中。你知道每天都有很多乱七八糟的电话，要你买这买那，要你捐钱，非常恼
人，女的就跟我说，她老接到这样的电话，还总是打给她先生的，她又不好意思挂断，
我就给她支了一招说，我听说美国人要是知道男主人不在家，接电话的是家里的佣人的
话，他们自己会挂电话，她问我，佣人怎么说，我说maid.
过些时间又有铃声了，她接了起来，只听她说：
He is not here. I am his mate.
我一愣，竖起耳朵仔细听。
I am his mate. 她嗓门显然加大了，然后气呼呼的挂上了，冲着我说，你这招不灵！
我差点喷饭，想想她也没说错啊，她是他老婆，当然就是mate了：）） |
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w********e
发帖数: 7186 | 16 Do you mean his bad situation in love issue caused his abnormal behavior |
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w****r
发帖数: 17566 | 17 【 以下文字转载自 Joke 讨论区 】
发信人: Peaceful77 (Serenity77), 信区: Joke
标 题: Stumpy and his wife
发信站: BBS 未名空间站 (Mon Jan 22 20:28:39 2007)
Stumpy and his wife Martha went to the state fair every year. Every year
Stumpy would say, "Martha, I'd like to ride in that there airplane."
And every year Martha would say, "I know Stumpy, but that airplane ride
costs ten dollars, and ten dollars is ten dollars."
One year Stumpy and Martha went to the fair and Stumpy said, "Martha, I'm 71
years old. If I don't ride |
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x*********6
发帖数: 3610 | 18 【 以下文字转载自 Hope 俱乐部 】
发信人: xiaobao1996 (1996!), 信区: Hope
标 题: This Cat Betrayed His Girlfriend (转载)
发信站: BBS 未名空间站 (Mon Oct 19 09:49:45 2009, 美东)
【 以下文字转载自 Joke 讨论区 】
发信人: yoooo (yoooo), 信区: Joke
标 题: This Cat Betrayed His Girlfriend
发信站: BBS 未名空间站 (Sun Oct 18 23:33:45 2009, 美东) |
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e*****n
发帖数: 3129 | 19 http://news.bbc.co.uk/2/hi/americas/8180116.stm
A US jury has found a man guilty of killing his sick 11-year-old daughter by
praying for her recovery rather than seeking medical care.
The man, Dale Neumann, told a court in the state of Wisconsin he believed
God could heal his daughter.
She died of a treatable disease - undiagnosed diabetes - at home in rural
Wisconsin in March last year, as people surrounded her and prayed.
Neumann's wife, Leilani Neumann, was convicted earlier this year.
The co |
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G******e
发帖数: 9567 | 20 【 以下文字转载自 TrustInJesus 讨论区 】
发信人: GetThere (不学有术), 信区: TrustInJesus
标 题: His (塞尔维特) Ashes Cry Out Against John Calvin (zz)
发信站: BBS 未名空间站 (Sun Dec 27 00:48:04 2009, 美东)
http://www.evangelicaloutreach.org/ashes.htm
His Ashes Cry Out Against John Calvin
Dan Corner
You are about to read an important part of church history from the
Reformation period that has been so concealed in our day that very few
people know the facts. Brace yourself for a shock.
On October 27, 1553 John Calvin, the founder of |
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A*******e
发帖数: 12486 | 21 【 以下文字转载自 Tennis 讨论区 】
发信人: daigua (欢喜就好), 信区: Tennis
标 题: federer put pressure on Murray in his after match interview
发信站: BBS 未名空间站 (Fri Jan 29 16:30:23 2010, 美东)
federer said british guy hasn't won a title for a very very long time, it's
not joking, i think, he is just give Murray some pressure so that he may not
play his best in the final, hehe. is this part of the 'tactic' federer
mentioned? |
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G******e
发帖数: 9567 | 22 http://www.evangelicaloutreach.org/ashes.htm
His Ashes Cry Out Against John Calvin
Dan Corner
You are about to read an important part of church history from the
Reformation period that has been so concealed in our day that very few
people know the facts. Brace yourself for a shock.
On October 27, 1553 John Calvin, the founder of Calvinism, had Michael
Servetus, the Spanish physician, burned at the stake just outside of Geneva
for his doctrinal heresies!(1) Hence, the originator of the popular do |
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J*******g
发帖数: 8775 | 23 This is a wrong understanding of God's omnipotence. See this:
James 1:13
When tempted, no one should say, "God is tempting me." For God cannot be
tempted by evil, nor does he tempt anyone;
Can you say God is not Omnipotence because he cannot be tempted? or tempt
others to do evil? No! Because, that's not his character and God is good!
God cannot do things that violates his very character. |
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T*******y
发帖数: 6523 | 24 Some ID mentioned some Ted videos before, where the author of the book "The
man who mistook his wife for a hat" gave a talk there. Thanks for bringing
this up.
Here's a link of this book:
http://www.arvindguptatoys.com/arvindgupta/wife-hat.pdf
This whole book is fascinating, especially the chapters 12-14 on Page 56-64.
Chapter 12 is about a man who can't remember anything due to severe amnesia,
and he has to make up stories to try to make sense, but never succeeds,
which leaves him in a frenzy.
... 阅读全帖 |
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r****o
发帖数: 105 | 26 跑跑western，用anti-His-tag 抗体看看有没有蛋白。另外Ni-NTA与his-tag
的结合非常不特异。你biacore的结果其实不可信。
很难相信300mM的imidazole还洗不下来.我觉得要么是根本没有蛋白表达
，或者没结合上去。如果是后者，你检查一下你的Ni beads是不是好的。 |
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n****e
发帖数: 1677 | 27 I don't think his ideas are reasonable. It is simply wrong.
You can't demand people to work 60 hours, while you only pay them for 40 hours
salary a week.
Poo himself may be working 60 hours even 168 hours a week, that's his problem.
I hate people saying like 'if you don't want to do science, just quit...',
doing science is just the same as any other works, why researchers have to
work overtime?
Hardworking doesn't simply mean working overtime. Hardworking means you make
good plans and finish wha |
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b******n
发帖数: 4225 | 28 取决于你的目的啦
Talon基于cobalt跟his tag结合，
优点是不太容易被lysate里面的巯基乙醇，DTT等还原
跟E.coli内源的含his的蛋白(如SlyD)结合力弱，纯化效果好
缺点嘛，就是有时候跟目的蛋白都不能结合
Ni-NTA的缺点差不多跟Talon反过来吧
NI-NTA 和 Talon mental beads 相比， 纯化的效果那个好些？
谢谢！ |
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w********r
发帖数: 1431 | 29 你可以在his tag和你的protein之间加一个Thrombin或者某个protease（比如3C）的切
点，
纯化好之后可以把tag切掉。
不过有时候有些蛋白在Ecoli表达很差甚至不溶，可以在his tag之前再放一个Trx帮助
蛋白表达，表达好之后所有的tag都可以再切掉的。 |
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r******t
发帖数: 629 | 30 我用agilent的Site-Directed Mutagenesis Kit在蛋白的N端加6 his tag。
分两步加的，一次加三个，结果第一次三个加得很容易，第二次的三个就是加不上，
primer也是靠agilent自己网上的primer design做的。
不知道有啥办法吗？
目前好郁闷啊。
另外，有没有人试过用3个his的tag来纯化呢？ |
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b******y
发帖数: 627 | 31 3 His is not close to be enough for purification. At least 6 His. If you can
, 8 or 10.
5' long primer containing: overhang sequence (e.g. GGAA); cloning site (make
sure in frame); His6 ---5' of your gene, should be the way to go as
abovementioned. |
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S*********s
发帖数: 304 | 32 // non-denature Tandem purification
3XFLAG + HA
or
ProteinA beads(IgG domain)+HA/FLAG
His or myc affinity and specific 不够~
//denature purification
His and biotag 会比较好~ |
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b******n
发帖数: 4225 | 33 可能蛋白的his-tag被包埋在蛋白里面了
如果不需要有活性的蛋白，直接变形了再去结合Ni-NTA
需要有活性的蛋白就要考虑将His-tag放到蛋白的另外一端试试看 |
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p****y
发帖数: 95 | 34 why use phosphate buffer for His-tag, it will precipitate Ni2+ and your
beads won't bind your His-tag protein anymore |
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k****l
发帖数: 279 | 35 有可能你的蛋白 his-tag 已经被降解掉了
可以做Western看看，但是要注意千分之一的蛋白有tag，western也会有信号，所以要
和另一个 his-tag 蛋白比较一下强度
threshold?
bead)？ |
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a****n
发帖数: 79 | 36 低浓度蛋白的His纯化之前用IP(antibody)浓缩? 在本身就很杂的细胞extract中增加蛋
白不是好的开始,比较routine的办法用离子交换树脂(Ion exchange)浓缩后脱盐再过
His柱,根据结果再继续纯化.前面的步骤不要加任何SDS,SDS不可能靠脱盐就能洗脱的,
会严重干扰后续纯化,最后也很难复性. |
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s*******e
发帖数: 44 | 37 这有什么典故么?
http://www.stanford.edu/group/dlab/papers/KD%20Nature%20Profile
In 2006, Boyden started his own lab at the Massachusetts Institute of
Technology in Cambridge. Rumours emerged that his relationship with
Deisseroth was souring. Neither is keen to discuss
the details, but in 2007, the two groups published separate papers on
halorhodopsin, an ‘off-switch’ opsin that they had previously studied
together. “We didn’t know Ed was working on that until we sent our paper
to Nature,” says Zhang. “... 阅读全帖 |
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H****s
发帖数: 301 | 38 一般而言，抗体识别的是6XHis的C端的三个His。特异性应该是非常好的，因为在第6位
His之后加一个氨基酸，抗体就不再识别了。所以，误伤的机率不是很大。 |
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t*****e
发帖数: 224 | 39 Part 2
II. The Later Marxism
Marx worked out his system of theory in the main during the third quarter of
the nineteenth century. He came to the work from the standpoint given him by
his early training in German thought, such as the most advanced and aggressive
German thinking was through the middle period of the century, and he added to
this German standpoint the further premises given him by an exceptionally
close contact with and alert observation of the English situation. The result
is that |
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t*****e
发帖数: 224 | 40 Part 2
II. The Later Marxism
Marx worked out his system of theory in the main during the third quarter of
the nineteenth century. He came to the work from the standpoint given him by
his early training in German thought, such as the most advanced and aggressive
German thinking was through the middle period of the century, and he added to
this German standpoint the further premises given him by an exceptionally
close contact with and alert observation of the English situation. The result
is that |
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d***a
发帖数: 128 | 41 Let us discuss Dr Geof A Ozin in University of Toronto. He publised many
papers in Adv Mater. Some in Nature/Science and Natural-series, less in JACS
and NL. But he is eay to publish his paper in Adv. Mater.
Also, I think his book was used as textbook in many nano-courses. |
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m****s
发帖数: 18160 | 42 ☆─────────────────────────────────────☆
nohate (两只东洋参) 于 (Wed May 20 10:49:55 2009) 提到:
投诉人(ID):
nohate
投诉对象及职务(限版主）:
yyber, stock bm
投诉标题:
use his bm power to ban id unreasonably
投诉目标(更改处理决定/更改板规/弹劾板主...):
更改处理决定
弹劾板主
投诉理由及证据:
1. it is up to complain board to decide if his reason makes any sense
I don\'t. I believe if an id does not pa, ga, he has the freedom of posting
any topic. My post is not promoting any stock, it is just a discussion.
yyber\'s post clearly stated that he is tired of |
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p*******y
发帖数: 4518 | 43 http://www.change.org/petitions/justice-for-jairo-who-was-murde
On Thursday, May 30, Jairo Mora Sandoval, a 26-year-old conservationist, was
kidnapped by sea turtle poachers. The next morning, his body was found on
the beach where he had worked to protect endangered leatherback sea turtles.
The Costa Rican government must respond to this shocking murder with swift
action that sends a clear message to all sea turtle poachers that their
actions will not be tolerated.
My name is Chris Pincetich and... 阅读全帖 |
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w*******y
发帖数: 60932 | 45 Newegg has HIS iCooler V H583FN1GD Radeon HD 5830 1GB 256-bit GDDR5 PCI
Express 2.1 x16 HDCP Ready CrossFireX Support Video Card w/Eyefinity for $
159.99 AR w/ Free Shipping.
+ Free Gift: HIS Gift - Call of Duty: Modern Warfare 2 Redemption Coupon (
value:$39.9 9)
Link
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w*******y
发帖数: 60932 | 48 HIS Radeon HD 6970 2GB (256bit) GDDR5 Eyefinity 2x Mini-DisplayPort HDMI 2x
DVI (HDCP) PCI Express X16 2.1 Video Card Retail (RoHS) H697F2G2M
Great deal on an awesome card. About $100 less than you will find it
anywhere else. Hurry before they are gone.
Link:
http://www.amazon.com/HIS-Eyefinity-Mini-DisplayPort-Express-H6
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