f******e
发帖数: 125 | 1 请问用HISTINE TAG 纯化蛋白.这个TAG会对蛋白性质影响大吗?
多谢了 |
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l*****m
发帖数: 98 | 2 1, the sample you get will be those proteins without histine tag. the his-
tagged protein only comes out when you wash them with imidazole
2, i think they will still have the activity if it is folded
3, i donot know. |
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n***w
发帖数: 2405 | 3 虽然自己没用过这个tag，但是看到n多组用6xHis tag做fusion protein，应该可以搞
吧。 |
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g***y
发帖数: 205 | 5 如果你的蛋白是histine kinase，我觉得最简单的实验应该是in vitro
phosphorylation.
IP |
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