c*****a
发帖数: 8 | 1 I am planning to apply for a position at the NIH human genome institute, but
found that there is only one Chinese PI. And current director, Eric Green,
and former director, now NIH director, Francis Collins's labs never had
Chinese. Kind of worry about fairness. Any insider providing some opinions
would be really appreciated. |
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h******y
发帖数: 1374 | 2 请问有用过OriGene的Trilencer-27 Human siRNA的同学吗？请问效果咋样啊？
3个unique 27mer siRNA duplexes 再加一个siRNA control是$390，好像比别的公司的便宜不少。 |
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y******8
发帖数: 1764 | 3 This would cost you half million. Insect cells work fine with human protein. |
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y***e
发帖数: 6082 | 4 哪家生物试剂公司可以买到，只要最普通的human plasma就行？多谢了 |
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h**********r
发帖数: 671 | 5 Title:
Functional characterization of eight human cytochrome P450 1A2 gene variants
by recombinant protein expression
The Pharmacogenomics Journal 10, 478-488 (December 2010) | doi:10.1038/tpj.
2010.2
My email is m******[email protected]. Thank you so much! |
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q**********0
发帖数: 335 | 6 请教版上高人：哪一种human cellline,有完整 Rb-E2Fpathway,但是没被任何病毒蛋白
转化的？非常感谢！ |
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l*******8
发帖数: 1745 | 7 we do human flow here. the tech usually run sample on the same day when they
get the blood sample. |
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R****n
发帖数: 708 | 8 I don't know much about biology.I want to use salmon DNA as a carrier, but I
don't know if Salmon and human may share similar sequence for certain genes
which may affect PCR in the down stream applications. |
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s******g
发帖数: 52 | 9 Is there any difference between yeast and human cells in DNA repairing?
Thanks for any hint or reference. THX. |
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t******y
发帖数: 716 | 10 祁鸣: 教授、博士生导师、美国医学遗传学学院院士
q**********[email protected];
1982年于华南师大获生物学学士学位；师从谈家桢/刘祖洞，1985年于上海复旦大学获
遗传学于生物工程学硕士学位； 1986年考取CUSBEA第5期赴美，1991年在美国匹兹堡大
学获分子生物学博士学位， 1998年毕业于美国西雅图华盛顿大学医学院医学遗传学主
任专修班，经全国统考获取美国临床分子遗传学执业执照。历任美国罗切斯特大学医学
院病理与临床检验系助理教授、副教授，1999年入选为美国医学遗传学学院院士，2006
年曾任美国哈佛大学遗传与基因组学中心顾问，分子医学实验室代理主任。目前为浙江
大学遗传与基因组医学实验室主任、教授，中科院北京基因组学研究所兼职研究员，美
国罗切斯特大学医学院病理与临床检验系兼职副教授。2007年获浙江省 “钱江人才”
资助。全国基因健康产业专业委员会首席专家、国际人类基因变异组计划中国区总协调
人、国际经济合作与发展组织（OECD）基因诊断国际标准专家组成员，《Human
Mutation》和《ANE》等国际杂志编委，并为多种国际杂志审稿。 |
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h*****G
发帖数: 113 | 11 human embryonic stem cell differentiated的细胞cell surface marker staining
需要block Fc receptor吗？
如果需要，一般怎么做？
谢谢。 |
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S**o
发帖数: 447 | 12 哪儿能得到18s RDNA的human reference library？
可以用它与NGS数据对齐吗？ |
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S**o
发帖数: 447 | 13 anyone knows the location of 18s rDNA for human?
how to know the location?
Is this information already known?
Does it change? |
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T**********y
发帖数: 559 | 14 最近做 overexpression of transgene in human primary T cells.
大家觉得那种 transduction vector好：Lentiviral vs Adenoviral? or other
suggestions?
多谢。这个试验很重要。 |
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s**********y
发帖数: 80 | 15 Anyone can suggest good human MMP-2 antibody and recom protein? Thanks! |
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f*******a
发帖数: 671 | 16 我现在有Human和Mouse的Microarray data,想相互比较基因或者信号通路的表达差异，
请问都有什么方法可选？ |
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a**********e
发帖数: 197 | 17 除了放狗
有没有什么网站有更新较快的job posting？
或者，做human genetic的一般都上哪里去post job opening？
谢谢了 |
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z*******6
发帖数: 679 | 18 我们这human genetices的SLE方向还是比较牛逼的。。。欢迎加入。。。哈哈哈。。。 |
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b****r
发帖数: 17995 | 19 american journal of human genetics和nature genetics好像经常刊登广告
如果去ASHG的annual meeting，招人广告就更多了 |
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a***y
发帖数: 19743 | 20 今天早些时候 看到了原文
感觉对研究trauma， burn和sepsis的影响大一些。
it's not the end of the world.
been
human |
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s******9
发帖数: 283 | 21 He is everywhere...
“The Human Genome Project was on the order of about $300 million a year for
a decade,” said George M. Church, a Harvard University molecular biologist
who helped create that project and said he was helping to plan the Brain
Activity Map project. “If you look at the total spending in neuroscience
and nanoscience that might be relative to this today, we are already
spending more than that. We probably won’t spend less money, but we will
probably get a lot more bang for the buck... 阅读全帖 |
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P****d
发帖数: 564 | 22 Some of the money will be wasted for sure for the hype. But if they're
willing to take the time and start small, like the human genome project did,
they can do good. It's definitely gonna be more challenging than the
genome project because there's no "complete answer" in this case. |
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v****a
发帖数: 388 | 23 人类的很多疾病似乎都跟valves/sphincter有关, 心脏, 消化系统de阀门关不严,反流.
If stem cell can help to create/rebuild strong human body valves, many
diseases can be cured. |
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m****a
发帖数: 427 | 24 请问大家有养human primary endothelial cells from bone marrow and core blood
的经验吗？有的话，能不能share一下你们用的什么media和方法呀，谢谢啦 |
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x******m
发帖数: 736 | 25 human的，找了几个，miRanda什么的，很久都没跟新。 |
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Z******t
发帖数: 206 | 26 向iPS的大牛们求教,如果想看一个基因的knock out 会不会影响human iPS cell
generation 的efficiency, 用什么donor cell做iPS最快最容易看到colonies?
reprogramming factors是不是用lentiviral Oct4,Sox2,Klf4,c-myc 效率最高.
多谢. |
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l***y
发帖数: 638 | 27 use male human neonatal fibroblast line. stemgent sells it. |
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r**********e
发帖数: 587 | 28 我做关于human complex disease尤其是neurological disease的genomics/
bioinformatics/personalized medicine的研究，想请问相关的著名会议有哪些呀？
谢谢 |
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a******a
发帖数: 283 | 29 we need to develop protocols for extracting DNA off human/mouse tissues and
wonder where we can order some. Any input would be greatly appreciated! |
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a******e
发帖数: 119 | 30 RT, 我想从reactome或者kegg（其他的database也可以）下载human 所有的pathways以
及每个pathway所包含的genes。 请问这个应该如何批量下载？ 谢谢！ |
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t******n
发帖数: 33 | 31 First, thank you very much for any suggestions on this problem.
I am running ADCC assay with human NK cells activated by IL-2 overnight. I
got ~100% killing of the target cells when cultured with activated NK cells
even without antibody, please refer to the data below.
________________________________________________________________
Target cells were labeled with Europium.
Target cells+ Effector cells (no antibody treatment)
average 338662
Target cells+ Effector cell... 阅读全帖 |
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f*****m
发帖数: 24 | 32 I am going to in vitro differentiate TH17 from human PBMC. Does anyone has a
reliable method that has a good yield? I know it is a very tricky field.
Thanks |
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k********n
发帖数: 756 | 33 I am planning to create virtue diseases by CRISPR knockin mutationS in human
iPSC lines.Anybody can give some references and thoughts? |
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I****p
发帖数: 101 | 34 http://news.sciencemag.org/scientific-community/2015/12/inside-
两个问题：
1）前景和问题争辩
2）谁会的若奖
... The long shadow of eugenics ... the three scientists widely expected to
share a Nobel Prize for a new DNA-changing technology, commonly called
CRISPR.
All was on display—and streaming live online—at the International Summit
on Human Gene Editing, which concluded yesterday here at the National
Academy of Sciences (NAS). |
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s********n
发帖数: 248 | 35 我想从加州寄培养中的human T cells到德州休斯顿，大概有70个million T cells。可
以做same day delivery 或者overnight shipment，想请教一下大家，是放在冰上寄比
较好，还是room temp就可以了？如果是same day delivery，是不是可以room temp寄
？还是不管是那种寄送方法，都放冰上寄比较好？
谢谢大家了先！ |
|
发帖数: 1 | 36 phd做些生信数据分析，博后想换方向，以后去工业界，是做cancer genomics还是去
human genetics以后更容易去pharma/biotech公司？这两个我感觉有时候挺像的，都做
variant calling and functional interpretation, 也许是我的理解太浅薄了，区别
主要在哪里呢? 哪个未来更有前景？ |
|
发帖数: 1 | 37 恩，我感觉挺像的。。
看上了几个老板，其中一个做human genetics的是个MD，自己有病人样品，主要做
exome和WGS，后续做mouse model实验，paper档次应该更高一些
一个做cancer genomics的是个PhD，主要是做血液病和breast cancer的WGS， 好像实
验做
得少些。 paper也不错，稍微弱一些 |
|
发帖数: 1 | 38 如题，不知道human genetics跟别的领域比怎么样？ |
|
发帖数: 1 | 39 哪位朋友可以下载到human mature miRNA全序列的table，谢谢 |
|
发帖数: 1 | 40 有人了解匹大公卫下面 human genetics这个专业吗 有mph和phd两个可选 求过来人说
说怎么样？ |
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M**********o
发帖数: 203 | 41 帮朋友询问。她是大四的学生，英语专业的，想来美国读Human resource的master，没
有考GMAT，不知道能申请到什么学校吗，GMAT的成绩是必要的吗，只要有AD就好。请知
道的朋友们推荐写学校吧。 |
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p*****t
发帖数: 3693 | 42 Frankly, for someone without work experience, a master degree in Human
Resources is useless. Most good programs would require applicants to have at
least several years of work experience. |
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A*L
发帖数: 2357 | 43 我认识有人没有工作经验，本科毕业后继续Master，然后就是读的Human Resource的，
一般州立大学毕业的，无绿卡的，一样找到工作。他现在是外派中国，负责招聘，时不
时再回总部。
什么东西也都是看机遇，没有绝对，也没有巧合。
at |
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g********t
发帖数: 88 | 44 Ipad 里的Siri真的神了！
码工们怎么看，在计算机向human intelligence方向发展有多大空间？
值不值得投身进去？
谢谢！ |
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p*********n
发帖数: 2 | 45 AI PhD求教啥叫human intelligence |
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x**c
发帖数: 1435 | 46 such program trains you to be a human resource manager who runs job interviews
, trains new employees,and takes care of team building, it is a people-
orientated job. you will deal with people everyday. |
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w******3
发帖数: 32 | 47 logistics 和human resources development |
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m**********l
发帖数: 18 | 48 本来想的研究是 在游戏（比如CS）当中用实际转头在控制第一人称画面的转动。 基本
也就是先弄一个类似眼镜大小的显示屏显示电脑画面，像眼镜一样戴着，然后连接到肩
膀上的仪器，用来测头转动的角度，并根据此角度改变显示屏的画面，以做到逼真模拟。
但是搜索了几个学校(standford,berkeley等等)的human computer interface(HCI)方
向都完全没有搞这个的，请问是因为
1）有人已经做出来了？
2）项目太小太简单弱智，随便本科生都能完成，不用放主页上？
3）不属于EE？
4）其他？
虚心请教，谢谢 |
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d*******1
发帖数: 1 | 49 有3个Ph.D.的RA机会
1. Dynamics and Controls of Umanned Air Vehicle (UAV) 在BYU, 是ECE. 主要是做
一些动力学及控制方法,还有硬件实现.老板是老美少壮派,学术水平应该还可以.
2. Data fusion/Processing, Sensing, 模式识别及一些简单的硬件设计用在医疗领域
. 在Univ of Utah, 是ME. 先让当TA, 老板刚毕业不久,具体项目还没拉到
3. Digital human modeling,好像叫数字(虚拟)人建模和仿真,主要做人的动力学,运动
学建模和一些优化吧, 这个似乎方向比较新,在Univ of Iowa, 是ME, 老板是
distinguished,应该还比较牛吧. 就是这个方向感觉...
不知道这三个方向哪个相对好些? 对将来工作来说呢? 请大家指点迷津, 矬子里拔将军
也拔一个出来. |
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s*******w
发帖数: 53 | 50 一朋友，大龄 （30＋）， engineering background, 现欲转phd in Human resource
education,
好好石油工程师不干，来北方读这个，说以后回去要做professor in business school
是不是神经病？ |
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