s****e 发帖数: 2934 | 1 【 以下文字转载自 Chemistry 讨论区 】
发信人: whglc (whglc), 信区: Chemistry
标 题: 问个ATRP前initiator immobilization的问题
发信站: BBS 未名空间站 (Sat Jun 30 21:49:36 2007)
最近在silicon wafer上通过ATRP长polymer,先要immobilize合成好的initiator(就是
Matyjaszewski文献中的11-(2-Bromo-2-methyl)
propionyloxyundecenyltrichlorosilane)。一般固定initiator大都在tolune里,我
做了也没问题(我加了TEA)。但现在因为特殊需要必须在极性溶剂如DMF中做,这样固
定initiator的效率大大下降了,长2天的效果都远不如tolune中的好。有没有什么办法
克服?比如有没有别的什么极性溶剂比较好?
另外,反应前silicon wafer表面我用UV/O3照30min,这一步是让表面产生SiO2,照射强
度应该够了吧。 |
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M*******a 发帖数: 15 | 2 One reserach fellow/scientist position is immediately available in the
National University of Singapore (www.nus.edu.sg). The two-year project is
co-funded by Singapore Economic Development Board (EDB) and GlaxoSmithKline
(GSK), focusing on developing green biochemical engineering technologies for
biopharmaceutical production. Major deliverable is in manpower training for
pharmaceutical industry of Singapore and the others are standard research
deliverables such as journal manuscripts. Upon comp... 阅读全帖 |
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M*******a 发帖数: 15 | 3 One reserach fellow/scientist position is immediately available in the
National University of Singapore (www.nus.edu.sg). The two-year project is
co-funded by Singapore Economic Development Board (EDB) and GlaxoSmithKline
(GSK), focusing on developing green biochemical engineering technologies for
biopharmaceutical production. Major deliverable is in manpower training for
pharmaceutical industry of Singapore and the others are standard research
deliverables such as journal manuscripts. Upon comp... 阅读全帖 |
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w******e 发帖数: 1187 | 4 请教:用cell-free expression synthesize peptide/protein, 要求newly synthe-
sized peptide/protein自动被immobilize到beads上,并达到尽量高的copy number。
最直接的方法大概是在gene里加个FLAG/HA的sequence,beads上coat FLAG/HA
antibody。但Ab个头太大担心copy number上不去。
请教有什么alternative method?比如his6-peptide的coverage会不会更高?
比如有没有site-specific biotinylation的reagent可以跟cell-free expression
合用?
非常感谢! |
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h**********r 发帖数: 671 | 5 Title: Immobilization of Lactococcus lactis to cellulosic material by
cellulose-binding domain of Cellvibrio japonicus
DOI: 10.1111/j.1365-2672.2010.04757.x
Journal of Applied Microbiology
Volume 109, Issue 4, pages 1274–1283, October 2010
My Email is m******[email protected]. Thank you so much! |
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M*******a 发帖数: 15 | 6 One reserach fellow/scientist position is immediately available in the
National University of Singapore (www.nus.edu.sg). The two-year project is
co-funded by Singapore Economic Development Board (EDB) and GlaxoSmithKline
(GSK), focusing on developing green biochemical engineering technologies for
biopharmaceutical production. Major deliverable is in manpower training for
pharmaceutical industry of Singapore and the others are standard research
deliverables such as journal manuscripts. Upon comp... 阅读全帖 |
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d*******7 发帖数: 188 | 7 Journal: Biotechnol Bioeng. 1987 Oct 20;30(6):724-35.
Title : Modeling the kinetics of immobilized glucose oxidase.
Author : Parker JW, Schwartz CS.
Thanks for the help!!
r******[email protected] |
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W***y 发帖数: 305 | 8 2004年93Saab,38K miles。下午还开得好好的,晚上就打不着火了。车内顶部的灯有
时暗,有时又和平时一样。钥匙有时转不动,有时只听见CD player,车锁和车头什么地
方咔咔响。SID显示“immobilizer failure”,不知道是不是电池突然死了,还是明天
会自己好,不然得拖到Dealer那去了。
各位有什么建议,先谢过了! |
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S*********g 发帖数: 24893 | 9 33 COMMIT THE VIOLATION. IT IS NOT ENTRAPMENT FOR LAW ENFORCEMENT OFFICERS
OR
34 THEIR AGENTS MERELY TO USE A RUSE OR TO CONCEAL THEIR IDENTITY. THE
CONDUCT
35 OF LAW ENFORCEMENT OFFICERS AND THEIR AGENTS MAY BE CONSIDERED IN
DETERMINING
36 IF AN EMPLOYER HAS PROVEN ENTRAPMENT.
37 Sec. 8. Section 23-214, Arizona Revised Statutes, is amended to read:
38 23-214. Verification of employment eligibility; e-verify
39 program; economic development incentives; list of
40 registered employers
41 A. After... 阅读全帖 |
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z*h 发帖数: 773 | 10 Cutting it from discussion.
This cellulose-to-amylose biotransformation could be scaled up by increasing
the stability of CBP and PGP and decreasing their production costs in terms
of $/kg enzyme (Fig. S6) because this combined cellulose-hydrolyzing and
starch-synthesizing enzyme cocktail did not involve any labile coenzymes [e.
g., CoA and NAD(P)] (Fig. 1A); no glucose released from the cellulose was
wasted (Fig. 1); only two more enzymes were added and they can be re-used
easily by a magnetic ... 阅读全帖 |
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W*****B 发帖数: 4796 | 11 CBS News is live, 24/7
Get the most important news stories of the day. Download our app for iPhone,
iPad and Apple TV to watch for free
Your dreams could be an early clue to Parkinson's disease
Actor Alan Alda revealed that he has Parkinson's disease — and in an
interview Tuesday on "CBS This Morning," the award-winning actor
said an unusual dream helped lead to his diagnosis.
Alda, best known for his portrayal of Army Capt. "Hawkeye" Pierce in
the TV series "M*A*S*H, was dia... 阅读全帖 |
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p*i 发帖数: 1481 | 12 我的车是2002 corolla,打算给我的车买个keyless car remote。网上找到个地方发现
能买。上面有个说明:
You have 90 days warrantee on 2002 TOYOTA COROLLA keyless entry remote or
key blank, make sure your TOYOTA is equided with a keyless remote system or
immobilizer, transponder key system.
我不确定我的车是否有a keyless remote system or immobilizer, transponder key
system?怎么能查到呢?车是二手的,而且manual已经掉了。谢谢! |
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X***9 发帖数: 7385 | 13 immobilizer 是指钥匙里有个电子芯片,必须通过编程才能启动车,如果但是打了一把
相同齿的钥匙,点火后会自动熄火,但是门还是可以开。是honda车的标准配置(以前
honda是丢车率最高的一个品牌,因为它很保值,所以贼老是惦记,所以后来honda把所
有的车都作了immobilizer)
security system 在ex以上才有,比如说别人通过敲碎玻璃的手段把手伸到门里解锁,
车子会叫,把引擎盖打开车子也会叫。 |
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m***j 发帖数: 533 | 14 你这个到底是Keyless还是Key的?Keyless的话比较简单去ebay上淘一个remote,注意看
FCC ID必须match,然后买来之后去搜program instruction,网上一大堆,自己program好
就行了.Keyless里面的Mechanical Key你可以找个Automotive Locksmith给你cut,一般
就10刀.
如果是Key的话就麻烦一点因为有immobilizer的问题.一般immobilizer的program必须
去dealer,但是你可以自己买key fob,找auto locksmith给你cut key和program remote. |
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g********b 发帖数: 8461 | 15 那就只能怪你自己了,为啥不配一把额外的备用钥匙呢?老车如果没有immobilizer和
alarm的话你可以想办法把门打开然后接电线就能走,如果有immobilizer的话也没啥太
好的办法不过你这什么车啊dealer居然用vin配不出钥匙?我94年的civic和97年的E320
都是给个VIN就直接刻出钥匙的。 |
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g********b 发帖数: 8461 | 16 分车,有很多车有便宜的aftermarket key或者used key可以program上。实际上如果只
是blade key+immobilizer的话就算原厂的也不贵,贵的是program immobilizer这个很
多车只能dealer做的。 |
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C*****z 发帖数: 2050 | 17 请问有相关数据证明“偷车大部分都是为了卖钱”吗?
我听说过偷面包、偷佛经、偷虎符、偷自行车和偷宝马的,但Jean Valjean、康有为、
如姬、Antonio Ricci和John McClane并非因为东西值钱而偷。
说到值钱,是本田值钱还是同级别讴歌值钱?如果本田更值钱,是不是讴歌与本田相比
就不是“好货”?如果讴歌更值钱,然而讴歌失窃率低是不是就意味着本田防盗不好或
者讴歌不是“好货”?
请问有相关资料证明本田是“世界上第一个在其产品上使用immobilizer”的吗?本田
又是哪一年开始在其产品上使用immobilizer的呢?
我从未觉得本田不堪,我坐11路。您宣传本田失窃率高是觉得本田很不堪吗?那么您开
什么牌子? |
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d******k 发帖数: 76 | 18 source: http://heather.cs.ucdavis.edu/h1b.html
Overview:
The H-1B work visa is fundamentally about cheap, de facto indentured labor.
The tech industry lobbyists portray H-1B as a remedy for labor shortages and
as a means of hiring "the best and the brightest" from around the world.
Though I strongly support that latter goal, the lobbyists' "best and
brightest" claims are not valid.
The vast majority of H-1Bs, including those hired from U.S. universities,
are ordinary people doing ordinary work, ... 阅读全帖 |
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g*2 发帖数: 658 | 19 我也感觉像pre-stress fracture
诊断的话看看这个:
Diagnosis of a tibial or fibular stress fracture is suggested by such a
history in a patient with risk factors and the following clinical findings:
Pain localizes to one specific area
Local swelling and discrete bone tenderness are present
Pain increases with the impact from running or jumping
An inability to hop on the symptomatic leg for 10 repetitions (positive hop
test) without excessive pain is also suggestive of the diagnosis. In this
setting,... 阅读全帖 |
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G***a 发帖数: 27294 | 20 你说的这个传记,我看过原文的每一个字。原文说了,她是blindingly beautiful, 所
以immobilize the air of the room
这段话来自肯尼迪的聚会,就是说她超级漂亮的意思。
事实上,我自己也写过关于梦露故事,看了好多资料。
http://www.mitbbs.com/article_t/Love/31453983.html
她这一辈子,漂亮是最大的魔咒,无法忽视的。
我不是说你观点错,而是例子太不恰当了。用一个世界级美女来例证"好看不好看无所谓?"
而且对原文断章取义。原文就是说她超级漂亮的意思。。。
没有说脸,是用了衬托的修辞手法。原文没提半句她有气场的意思在。
Immobilize the air是修辞说她极其美。下一段就说肯尼迪走近她。
这段是铺垫肯尼迪出现 |
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z*h 发帖数: 773 | 21
==> Current power density is still lower than directed methanol fuel cell
but far higher than theoretical power output of microbial fuel cells. The
maximum power density of sugar battery could be increased by 100 folds by
integration of several things together. It is why we never work on microbial
fuel cells.
and bio-active electrode, i.e. conductive bio-enzyme mimic structure or
==> No one claim that sugar biobattery is new. In this paper, we have two
things new:
(1) the first complete oxidati... 阅读全帖 |
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d******k 发帖数: 76 | 22 【 以下文字转载自 JobHunting 讨论区 】
发信人: dumbLuck (dum luck), 信区: JobHunting
标 题: Professor Norm Matloff's H-1B Web Page: Cheap H-1B labors
发信站: BBS 未名空间站 (Fri May 2 11:51:20 2014, 美东)
source: http://heather.cs.ucdavis.edu/h1b.html
Overview:
The H-1B work visa is fundamentally about cheap, de facto indentured labor.
The tech industry lobbyists portray H-1B as a remedy for labor shortages and
as a means of hiring "the best and the brightest" from around the world.
Though I strongly support that latter g... 阅读全帖 |
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T**********t 发帖数: 1604 | 23 哦,我没比较过,因为我一般不需要把结合上去的蛋白再洗下来。
我就是看说明书上这么说的:
2.2 Release of Immobilized Biotinylated Molecules
The biotin-streptavidin bond is broken by harsh conditions. 5 mins
incubation at 65°C or 2 mins at 90°C in 10 mM EDTA pH 8.2 with 95%
formamide will typically dissociate >96% of immobilized biotinylated DNA.
Alternatively, boil the sample for 5 mins in 0.1% SDS for
protein dissociation.
Please note that proteins will be denatured by such treatment and Dynabeads
Streptavidin can not be re-used.
It has al |
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r*****1 发帖数: 805 | 24 My CCS protocol:
P.S.:多有重复,谨防遗漏,仅供参考。
Screening test: General—Lipid profile/ multi-vitamin; Elderly—DEXA scan&
Calcium& VitD/ Colonscopy or FOBT/ vaccination; F-- >18yo Pap smear; >50yo
Mammogram; reproductive age- folate; menopause- Lipid/DEXA/FOBT; M-- >50yo
PSA; sickle cell dz child-prophylaxis w penicillin till 5yo, CF-prophylaxis
w Abx
Prophylaxis: Pantoprazole, pneumatic compression stocking;
Acute abd w perforation: triple Abx- Gentamycin/ Ampicillin/
Metronidazole (口诀:阿扁举旗庆国庆)
E... 阅读全帖 |
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z******8 发帖数: 844 | 25 ☆─────────────────────────────────────☆
rhcrc11 (Rebel) 于 (Thu May 15 00:13:42 2014, 美东) 提到:
Step3考场壮烈回来。第一天挺累人,最后一个block甚至做到有心无力状,看着题反应
不过来,导致来不及做完。非常没有信心能否通过。所以match之前先考Step3的童鞋,
需要考前调整生物钟,坚持锻炼,提高耐力。
MCQ没有资格说,CCS有一点小感受,拿出来分享下。大家请轻拍砖。
复习资料:
UW 52 online cases : 基础。最好复习早起找高手一起过一遍,尽快摸熟软件,进入
状态。第二遍找一位水平相近童鞋过一遍,不断总结protocol. 考前迅速做一遍,熟练
运用protocol.
UW 41 offline cases: 我当时和partner每个case仔细做一遍,虽然不像online有反馈
,但对练临床思维,补充protocol非常有帮助。最后考试与41 case有异曲同工之处,
遇到复杂case也不慌神。
CD 6 cases: 最后再熟悉下考试软件。与UW相近,但... 阅读全帖 |
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i*********5 发帖数: 19210 | 26 http://www.houstonfootandanklesurgeon.com/heel-pain.html
Plantar Fasciitis
Plantar Fasciitis - inflammation in the sole of the foot.
Who gets it? Everyone. People who are active either at work, home, or
athletically are more likely to get plantar fasciitis. It is common in
runners and walker and in people who stand for prolonged periods of time.
Pain is located in the arch near the Heel. It is worse in the morning when
you get out of bed as well as after prolonged sitting.
The cause of plantar f... 阅读全帖 |
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a***o 发帖数: 3734 | 27 if it was immobilizer, would there be a message displaying?
The message board was clean, nothing showed up. I googled
volvo immobilizer, people always say "a message shows up..." |
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m**********3 发帖数: 706 | 28 Movie Review: Chosin
A A AComments (0) By Chuck Wilson Thursday, Sep 16 2010
CHOSIN This year marks the 60th anniversary of North Korea's invasion of
South Korea, an event that caught the United States and its allies off guard
. Although the U.S. ultimately prevailed over the Soviet-backed North
Koreans and their Chinese allies, the first year in this three-year war was
something of a death march for American soldiers, particularly in the brutal
Chosin Reservoir campaign. In this superb document... 阅读全帖 |
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y****i 发帖数: 1050 | 29 It was here that German forces encountered a unit equipped with the Soviet
KV heavy tanks for the first time. Gen. E.N. Soliankin's 2nd Tank Division
from Soviet 3rd Mechanised Corps attacked and overran elements of the German
6th Panzer Division[7][c][I] near Skaudvilė on 23 June. The Germans'
Panzer 35(t) tanks and antitank weapons were practically ineffective against
the Soviet heavy tanks—some of them were out of ammunition, but closed
with and destroyed German antitank guns by literall... 阅读全帖 |
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y****i 发帖数: 1050 | 30 It was here that German forces encountered a unit equipped with the Soviet
KV heavy tanks for the first time. Gen. E.N. Soliankin's 2nd Tank Division
from Soviet 3rd Mechanised Corps attacked and overran elements of the German
6th Panzer Division[7][c][I] near Skaudvilė on 23 June. The Germans'
Panzer 35(t) tanks and antitank weapons were practically ineffective against
the Soviet heavy tanks—some of them were out of ammunition, but closed
with and destroyed German antitank guns by literall... 阅读全帖 |
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g****t 发帖数: 4493 | 31 2014年04月03日
3月27日,中国国家主席习近平访问法国,在巴黎出席中法建交50周年纪念大会上发表
演讲,提到拿破仑的名言:“中国是一头沉睡的狮子,当这头睡狮醒来时,世界都会为
之发抖。”此话在国内流传甚广,从中学生表达爱国主义的蹩脚作文,到专家们分析国
际关系的重磅评论,都不难见到这头狮子的身影。
“中国睡狮论”是一起横跨欧亚大陆,历时两个世纪的话语制造事件,中外学者都所提
及,拿破仑也时常被摆出来。但是他是否进行过这番预测,又在何种场合说了这番话?
仔细考据,说法纷繁复杂;中国究竟是否是一头狮子,沉睡或苏醒也表述各异。
2004年2月,《环球时报》发表过一篇小文章,题为《拿破仑“中国睡狮论”怎么来的
》。作者史鸿轩先讲述了1816年英国使臣阿美士德伯爵(William Pitt Amherst, 1st
Earl Amherst)拜访拿破仑一事,而后引述了这位法兰西英雄对中国的看法:“……中
国并不软弱,它只不过是一只睡眠中的狮子。……中国一旦被惊醒,世界会为之震动。
”这个故事听上去有板有眼,入情入理,可惜此文没有提供任何史料出处。
1980年代末,中央电视台曾播放曾引发争... 阅读全帖 |
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w***t 发帖数: 8175 | 32 By WASBIR HUSSAIN, Associated Press Writer – Tue Mar 23, 7:19 am ET
GAUHATI, India – The Indian military has a new weapon against terrorism:
the world's hottest chili.
After conducting tests, the military has decided to use the thumb-sized
"bhut jolokia," or "ghost chili," to make tear gas-like hand grenades to
immobilize suspects, defense officials said Tuesday.
The bhut jolokia was accepted by Guinness World Records in 2007 as the
world's spiciest chili. It is grown and eaten in India's northe |
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f********8 发帖数: 1417 | 33 India Graduates Millions, but Too Few Are Fit to Hire
BANGALORE, India—Call-center company 24/7 Customer Pvt. Ltd. is desperate
to find new recruits who can answer questions by phone and email. It wants
to hire 3,000 people this year. Yet in this country of 1.2 billion people,
that is beginning to look like an impossible goal.
So few of the high school and college graduates who come through the door
can communicate effectively in English, and so many lack a grasp of
educational basics such as re... 阅读全帖 |
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a***x 发帖数: 1303 | 34 http://www.local10.com/news/george-zimmerman-trial-witness-ship
During testimony, Bao said he was relying on his seven pages of notes and
the six pages of the autopsy report to testify. He said he did not remember
the specifics of the autopsy.
"I told you before, I tell you right now," Bao said. "I do not remember
anything."
While he did not want attorneys to see his notes, Judge Debra Nelson ordered
him to do so. The copies would later be destroyed, Nelson said.
Bao told Prosecutor Bernie de la... 阅读全帖 |
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C*I 发帖数: 4736 | 36 http://rt.com/usa/toxic-study-gmo-corn-900/
A leaked study examining genetically-modified corn reveals that the lab-made
alternative to organic crops contains a startling level of toxic chemicals.
An anti-GMO website has posted the results of an education-based consulting
company’s comparison of corn types, and the results reveal that genetically
modified foods may be more hazardous than once thought.
The study, the 2012 Corn Comparison Report by Profit Pro, was published
recently on the website... 阅读全帖 |
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t*******y 发帖数: 21396 | 37 讲到移民改革,共和党参院移民委员会主席Jeff Sessions新年刚过就来亲切关怀中国
PhD了:
http://www.mitbbs.com/article_t0/EB23/32224359.html
Sessions 主席在他的大作里特别提到中国博士学生了,诸位对共和党强烈支持给STEM
博士生发绿卡的作何感想啊?
Another major, yet often overlooked, provision in the [Senate immigration
bill] would grant automatic green cards to any foreign student who earns a
graduate degree in a STEM field, based on assertions that foreign graduates
of U.S. universities are routinely being forced to leave. Such claims are
incompatible with the evidence that suc... 阅读全帖 |
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b********n 发帖数: 38600 | 38 http://www.paulcraigroberts.org/2015/04/27/insanity-grips-weste
Just as Karl Marx claimed that History had chosen the proletariat,
neoconservatives claim that History has chosen America. Just as the Nazis
proclaimed “Deutschland uber alles,” neoconservatives proclaim “America
uber alles.” In September 2013 President Obama actually stood before the
United Nations and declared, “I believe America is exceptional.”
Germany’s political leaders and those in Great Britain, France, and
throughout Europe... 阅读全帖 |
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s*******1 发帖数: 16479 | 39 不会游泳应该被说成water immobile |
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d**i 发帖数: 1081 | 40 非母语的文艺创作能否逆袭
尚雯婕自《Nightmare》专辑开始,多次在专辑中收入了自己原创的英文,法文等非母
语歌曲。可以说用英文,法文填词,已经成了她的一个标志了。那么用非母语进行文艺
创作,究竟是如某些人诟病的那样是装逼行为,还是这确实是一种走向世界的方式呢?
用非母语进行文艺创作的作品,究竟能不能得到以该语言为母语的人们的认可呢?
下面,我打算从我有限的视野了解到的情况来个管中窥豹,仅作抛砖引玉,期望得到更
多的方家参与讨论。
我印象中,使用英语进行小说创作并获得了美国国家图书奖的哈金是第一个让我感到原
来中国人完全可以用非母语创作出美国人认同的小说作品的。他的成功让我意识到,非
母语并不是文艺创作的禁区,作品的好坏不取决于你使用哪种语言。
然后,还有一个著名的作家,就是创作了鼎鼎大名的《洛丽塔》的俄国人纳博科夫,他
本人也是一个移民,英语并非其母语。但是移民美国后,成为了大学英文写作课的教授
,还创作了世界名著《洛丽塔》。
互联网一搜,发现海外华裔用非母语创作取得成绩的还真不少,明镜周刊还评过一个十
大最有影响的海外华裔作家,并且主要都是文革后出国的。
10. 应晨(Ying... 阅读全帖 |
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发帖数: 1 | 41 以下内容仅供参考…
Brussels — I am in the quiet of a business lounge at a bustling European
airport. The coffee is strong and true, the croissants sinfully buttery, and
self-important men and women of all nationalities fuss about with their
laptops. And then I notice something: a prosperous Indian man of about 60,
overweight by the standards of a Brussels airport lounge but not of a New
Delhi 5-star hotel, is brought in on a wheelchair. The attendant leaves him
by a sofa seat and agrees to return when it... 阅读全帖 |
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w********2 发帖数: 632 | 42 Ferdinand Losses
The Battle of Kursk is, perhaps, the most famous tank battle in history. In
that battle, the Ferdinand assault gun was introduced, seemingly invincible,
with the most powerful tank gun fielded by the Wehrmacht to date. Due to
the relatively few numbers of Ferdinands manufactured, it is possible to
account for nearly all of them.
Ferdinands from s.PJA. 654 were sent to the railroad station of Ponyri, and
many of them met their end there. Tank.UW.ru has a list of the damaged tanks... 阅读全帖 |
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k**********4 发帖数: 16092 | 43 Lessons from Hale Ross’ life at Yale
A personal essay by Hale's father
JACK ROSS 5:47 PM, MAY 05, 2018
CONTRIBUTING REPORTER
Editors’ Note: Please be advised, the following piece includes sensitive
material relating to depression and suicide.
Several months ago, I received a “save the date” flyer about the Yale
graduation events this May. I will not be in attendance. On Oct. 30, 2016,
my son Hale, class of 2018, ended his life.
Hale’s death was a tragedy on many levels — a profound loss not o... 阅读全帖 |
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s**********e 发帖数: 33562 | 44 下面是英文wiki上门对意大利在战争中的表现的评价:
Allied press reports of Italian military prowess in the Second World War
were almost always dismissive. British wartime propaganda trumpeted the
destruction of the Italian 10th Army by a significantly smaller British
force during the early phase of the North African Campaign.[77][78] The
propaganda from this Italian collapse, which was designed to boost British
morale during a bleak period of the war,[79] left a lasting impression. The
later exploits of Rommel and Germ... 阅读全帖 |
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k*****r 发帖数: 21039 | 45 1. 距离不到200米吧,德军的PAK40 75毫米炮,居然不能洞穿鞋儿慢这个大胖子。
2. Tiger一般都是埋伏起来,远距离的就干掉米军坦克,44年米英军和德国坦克战的交
换比是4:1. 有必要冲出来和米军M4周旋吗?
3. 最后一战,Waffen SS这样的老兵油子,会端着枪往immobilize的坦克的机枪前冲,
而不是利用夜暗条件,打Panzerfaust ? 麻痹的哪怕只有一个班的人,几个
Panzerfaust,都能把pitt他们烧成灰。
“i have been to the concentration camp...yada yada", pitt同志真俗啊,给米尤
背书,这么不含蓄。 |
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