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b****n 发帖数: 311 | 2 你可以用自体细胞来源的ips啊,你可以预筛一遍去掉有autoantigen的细胞啊,再不
济还有免疫抑制剂啊。
搞研究本来就是不断发现问题解决问题的过程,你真信ips的autoimmunity是不可解决
的打击? |
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o**4 发帖数: 35028 | 3 所以说,他们应该用RNA诱导的IPS检查一下,应该很快就有paper follow这个了,
iPS前景很好,大家应该有信心 |
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p*****m 发帖数: 7030 | 4 这个没关系吧 iPS本来离真的用也很遥远的 等能用了你的bb也过了血液遗传病高发的年
纪了。
脐带血的问题是,数量很少只够给小小孩子治病的;能治的病很有限,而且如果bb有遗
传病,脐带血干细胞是没法用的(也带遗传病基因)。至于吧脐带血干细胞扩增,或者
修复遗传异常的技术,未必会比iPS出来的早 呵呵 |
|
y******8 发帖数: 1764 | 5 For iPS, you might get a cell line from the patient with or without known
mutation, and differentiate it into the disease-related tissue.
Then play with it and call it the disease model. The significance is you
might be able to set up hundreds of lines based on iPS techniques.
model |
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n********k 发帖数: 2818 | 6 Sure, I am with you...I was just reminding onco of those...but still both ES
and iPS for cure are hype, agree? But I am not underestimating the value
of iPS in research etc...It is huge and Shinya definitely deserves Nobel... |
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B******o 发帖数: 496 | 7 别试了。Cell stem cell那篇文章肯定要翻船的。
你只要看看他们的文章里说的iPS的效率就知道啦,80%,可能不?呵呵
另外,在此之前没有一篇文章报道说microRNA能当transcription factor用。他们这个
有些太离谱了
iPS |
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b***2 发帖数: 348 | 8 请教一下做ips的大牛们。
我主要是研究大分子投递的。因为ips很火,并且做的方法也算是有限,病毒效果好,
但是有风险,其他的方法据说效果差,我现在想设计一种方法,不用病毒,期望达到接
近病毒的效果。
现在的问题是我没有杀老鼠的protocol,这样自己搞MEF有难度,ATCC的又贵,现在我
的方法也不一定能成功,老板不见兔子不愿意撒鹰出去。我想问一下,初始的细胞材料
,包括做reprogram的和feeder有没有什么容易一些的途径搞到。 |
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i*********0 发帖数: 915 | 9 我想验证一下一个gene的KO能不能提高ips generation的效率,已有可以用OHT 做
induced deletion的MEFs,想问一下一个周期的ips generation一般多长?
谢谢! |
|
i*********0 发帖数: 915 | 10 我想验证一下一个gene的KO能不能提高ips generation的效率,已有可以用OHT 做
induced deletion的MEFs,想问一下一个周期的ips generation一般多长?
谢谢! |
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a**********4 发帖数: 15 | 11 版上各位大侠,我现在实验室刚开始从事IPS细胞的课题,以前实验室从来没人做过,
我查了一些文献,发现现在是不是都用一个Lentivral vecotor上挂4个gene来诱导IPS
细胞?
山中申弥的用4个retroviral的办法是不是已经不用了?
如果哪位大侠有好的protocol,请发站内信箱,5个包子的奖励。 |
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d********0 发帖数: 534 | 12 唉,ips的疾病研究大多还是骗人阶段,ips的安全性一日不清,永远这个课题都是胡说 |
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i*********0 发帖数: 915 | 13 如果从mouse ips clone pick up开始算P0,大家觉得一般越early passage (比如说
p2-5) 注射做chimera好,还是late passage (P20以后)注射比较好。或者说,这个
做chimera也是一个随机的事件,和上次cell上的一片说ips是一个随机的过程的review
一样。 |
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b********e 发帖数: 50 | 14 做iPS一般用原代细胞,体外最多可以传代50次? 表达3个基因的话,可能还没有做成
stable cell line,细胞就死翘翘了。做过iPS的同学建议一下呗。 |
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l******a 发帖数: 84 | 15 我的亲戚是国内目前发展强劲的制药公司的副总裁兼研发中心leader。目前有意向寻找
ips干细胞领域人才,最好是有iPS beta 细胞应用于糖尿病治疗方面的研究经验, 人
在加州更佳。可以考虑技术咨询,项目合作,或者人才招聘。亲戚11月要来加州,希望
能趁此机会面谈。有意者请联系[email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.getElementsByTagName("script");l=b[b.length-1].previousSibling;a=l.getAttribute('data-cfemail');if(a){s='';r=parseInt(a.substr(0,2),16);for(j=2;a.length-j;j+=2){c=parseInt(a.substr(j,2),16)^r;s+=String.fromCharCode(c);}s=document.createTextNode(s);l.parentNode.replaceChild(s,l);}}catch(e){}})();... 阅读全帖 |
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d********0 发帖数: 534 | 16 虽然有论文证明过ips细胞也有免疫原性,但对于高级动物来说,这种免疫原性极小,
至于干细胞治疗糖尿病是否有效,只能说现在的研究还没到那个份上
ips现在的临床应用还是很慢,主要是因为安全性问题,山中伸弥拿诺贝尔奖是有点名
不符实 |
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m******5 发帖数: 1383 | 17 问一下有没有什么比较好用便宜的ips generating kit?
需要用已有的genetic background and modified alleles to generate some ips
cell lines to study lineage specific differentiating.
病毒非病毒都可以。
不要太贵就行。 |
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发帖数: 1 | 18 很多复杂疾病搞不清楚病因,基本的遗传学都搞不清楚,尤其非编码区的;crispr解决
了这个问题
而依赖于实验室的cell line完全无法模拟真实人类细胞组织器官,ips也在解决这个问题
所以如果crispr+ips是不是算是疾病研究很大的一个跨越?尤其未来越来越强调
precision medicine,因人而异
当然了,最大的问题是in vitro study。
我估计现在很多人都在做这个吧?不知道作为未来事业的发展方向有多少前景?
大家来谈谈看法吧。 |
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w*******y 发帖数: 60932 | 19 MacMall has HP ZR24W 24" 1920x1200 S-IPS LCD Monitor w/ DisplayPort, DVI and
VGA inputs for $380 - $50 rebate = $330 with free shipping. Next best on
Google Products is $389.
Couple of months back it was $300 and was front page deal.
Searched and did not find any other posts.
Link to the page
Link:
http://www.macmall.com/p/6110671
spec from the seller site
General
Display Type LCD display / TFT active matrix
TFT Technology S-IPS
Built-in Devices USB hub
Width 21.9 in
Depth 9.3 in... 阅读全帖 |
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w*******y 发帖数: 60932 | 23 1. Go to mastercard market place.
2. Search for lenovo, you'll get a 5% off link which leads you tolenovo
corporate perks site:
http://shoplenovo.i2.com/SEUILibrary/controller/e/corporateperk
.
3. Configure your x220 or x220t, and add to cart.
4. Apply coupon code LENOVOVIP which gives you 15% off. (No additional
coupon codes will work)
5. Checkout as guest or your ID if you have any.
For x220, this is what you can get for $834.61:
i5-2520m (upgrade)
Windows 7 home premium 64 (downgrade)
IPS pre... 阅读全帖 |
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w*******y 发帖数: 60932 | 24 LG E2370V-BF 23-Inch Widescreen LED IPS monitor.
shipped and sold by amazon!
Link:
http://www.amazon.com/LG-E2370V-BF-23-Inch-Widescreen-Monitor/d
Super LED panel provides a slim profile and delivers amazing brightness
clarity
In Plane Switching or IPS controls the liquid crystal display to help keep
distortion at a minimum
Full HD 1080p resolution (1920x1080) gives it superior picture quality over
standard resolution
5,000,000:1 contrast ratio ensure your content looks spectacular
RGB HDMI DVI ... 阅读全帖 |
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w*******y 发帖数: 60932 | 26 Not sure if this is a repost. I searched and didn't see anything.
---------------------------------------------------------
Dell Home has the Dell XPS 8300 Intel Core i7-2600 Quad-Core Sandy Bridge
Desktop Computer w/ 24" UltraSharp IPS LCD Monitor for a low $999.99 Free
Shipping after Coupon Code: 1006G46FQ7NZ1J (Exp 9/12). Tax in most.
24" Dell UltraSharp U2412M IPS; Intel Core i7-2600 3.4GHz Quad-Core Sandy
Bridge; 8GB RAM; 1TB HDD; Blu-Ray; 802.11n + gigabit; Windows 7 Home Premium
; ATI Rad... 阅读全帖 |
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w*******y 发帖数: 60932 | 27 Dell Home:
http://configure.us.dell.com/dellstore/config.aspx?c=us&l=en&cs
has a well-equipped XPS 8300 Desktop + 24" Dell U2412M Ultrasharp IPS
1920x1200 LED Monitor
$1593.99 - $594 w/ code 1006G46FQ7NZ1J
= $999.99 (or $970 w/ DPA)
Free shipping
Specs for Dell XPS 8300 Desktop: Intel Core i7-2600 3.4GHz
8GB DDR3 Memory
1TB 7200RPM Hard Drive
1GB ATI Radeon HD 6670 GDDR5
8X Blu-ray Combo Drive
Dell 1501 Wireless-N
Windows 7 Home Premium 64-bit
Specs for Dell U2412m Monitor: Resolution: 1920x1200... 阅读全帖 |
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w*******y 发帖数: 60932 | 28 Dell Small Business has the Dell Vostro 460 Mini Tower Intel Core i5-2400
Quad-Core Sandy Bridge Desktop Computer w/ Dual 22" Dell UltraSharp IPS LCD
Monitors for $657.30 + Free Shipping
Tax in most.
DUAL Dell UltraSharp U2211H 22" IPS LCDs; Intel Core i5-2400 Sandy Bridge
Quad-Core 3.1GHz; 2GB RAM; 320GB HDD; DVD-ROM; gigabit; Windows 7
Professional; 1yr warranty; 15mo Trend Micro Business Security; keyboard/
mouse
CLICK HERE:
http://configure.us.dell.com/dellstore/config.aspx?cs=04&dgvcod
Sele... 阅读全帖 |
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w*******y 发帖数: 60932 | 30 Dell Home has their XPS 8300 Desktop + 24" Dell U2412M UltraSharp IPS LCD on
sale for $900 after applying coupon code 3PDC6CD87JFD9X at checkout.
Shipping is free.
$50.00 more than Black Friday -
Desktop Specs:
Intel Core i7-2600 3.4GHz
8GB DDR3 Memory
500GB 7200 RPM SATA Hard Drive
16X CD/DVD burner (DVD+/-RW)
AMD Radeon HD 6670 1GB DDR5
Dell 1501 Wireless-N PCIe Card
Microsoft Office Starter
Windows 7 Home Premium 64-Bit
Monitor Specs:
Resolution: 1920x1200
Panel Type: IPS
Response Time GTG: 8... 阅读全帖 |
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w*******y 发帖数: 60932 | 31 20" 4:3 1600x1200 IPS Monitor. Factory Refurbished
2 DVI and 1 VGA input
8ms gtg refresh rate
178/178 degree horizontal/vertical viewing angles
79.99 +
15.00 Shipping and Handling
94.99 Total
Before you flame me, let me explain why this is a good deal for certain
people....
1. Some people want/need a larger 4:3 panel for certain applications.
2. This is an IPS panel, not a TN panel. That means it has much better
viewing angles, and color reproduction than anything you're likely to find
in this p... 阅读全帖 |
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w*******y 发帖数: 60932 | 32 Not sure if has heard about this, but apparently two Korean companies are
selling extremely cheap S-IPS 27" 2560x1440 monitors for around $400-450
shipped to the USA (they're around $250 if you're in South Korea).
Link:
http://www.ebay.com/itm/CATLEAP-Q270-LED-2560X1440-WQHD-27-DVI-
#ht_8665wt_1154" rel="nofollow" target="_blank" onclick="_gaq.push(['_
trackEvent', 'thread', 'click', '4064178 - catleapshimian-27-sips-2560x1440-
monitor-for-378-shipped-ebay']);">http://www.ebay.com/itm/CATLEAP-Q... 阅读全帖 |
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G*******s 发帖数: 10605 | 33 【 以下文字转载自 SmartShopper 俱乐部 】
发信人: GoRockets (火箭加油), 信区: SmartShopper
标 题: Monoprice 27" IPS-Glass Pro LED Monitor $398.36
发信站: BBS 未名空间站 (Sat Sep 28 11:18:54 2013, 美东)
Monoprice via Amazon has Monoprice 27" IPS-Glass Panel Pro LED Monitor WQHD
2560x1440- 440cd/m2 - HDMI 1.4 / DVI / VGA / DisplayPort 1.2 w/Built in
Speaker for $398.36 with free shipping.
http://userquote.com/forum/index.php?topic=2020.msg2346#msg2346 |
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G*******s 发帖数: 10605 | 34 【 以下文字转载自 SmartShopper 俱乐部 】
发信人: GoRockets (火箭加油), 信区: SmartShopper
标 题: ViewSonic 22-Inch IPS LED Monitor $116.99
发信站: BBS 未名空间站 (Mon Dec 9 23:37:40 2013, 美东)
Amazon has ViewSonic VX2270SMH-LED 22-Inch IPS LED Monitor (Frameless Design
, Full HD 1080p, 30M:1 DCR, HDMI/DVI/VGA) for $116.99 with free shipping.
http://userquote.com/forum/index.php?topic=2172.msg2537#msg2537 |
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G*******s 发帖数: 10605 | 35 【 以下文字转载自 SmartShopper 俱乐部 】
发信人: GoRockets (火箭加油), 信区: SmartShopper
标 题: Dell U2414H 24" HD IPS LED Monitor $244.99
发信站: BBS 未名空间站 (Tue Mar 11 02:44:48 2014, 美东)
Dell Home has Dell U2414 24" IPS LED Monitor for $244.99 with free shipping.
http://userquote.com/forum/index.php?topic=2331.msg2738#msg2738 |
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G*******s 发帖数: 10605 | 36 【 以下文字转载自 SmartShopper 俱乐部 】
发信人: GoRockets (火箭加油), 信区: SmartShopper
标 题: Dell IPS 22-Inch Screen LED-Lit Monitor $122.99 Free Shipp
发信站: BBS 未名空间站 (Tue Sep 16 01:24:41 2014, 美东)
Amazon has Dell IPS 22-Inch Screen LED-Lit Monitor for $122.99 with Free
Shipping.
http://userquote.com/forum/index.php?topic=2539.msg3119#msg3119 |
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a****o 发帖数: 1786 | 37 【 以下文字转载自 Biology 讨论区 】
发信人: demoner (回不去的叫故乡), 信区: Biology
标 题: 今天Nature 5篇iPS的paper...
发信站: BBS 未名空间站 (Wed Aug 26 14:03:33 2009, 美东)
好像每个搞iPS的牛实验室都搞了一篇,西班牙人比较牛,居然搞了两篇。。
以后Nature生物方面的文章只发stem cells的算了。 |
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c*********7 发帖数: 7607 | 38 我想卖的物品:
Used T60p in perfect condition
单张面值:
差不多1500吧
可接受价格(必须明码标价!):
850
物品新旧要求:
used,in great condition
邮寄方式要求:
your label
买卖双方谁承担邮寄损失(Required if not code only):
default, before/after,
付款方式说明:
BOA, Paypal, ING
其他补充说明:
屏幕是 2058*1536的 QXGA IPS
内存3G,硬盘640G,256G GPU显存。DVD/R, second drive bay免费送
广告的有效期:
until gone
物品来源(Required for All Cards!):
orginally IBM, 屏幕组装
我的联系方式:
bbs mail
Warranty期限:
No warranty;guaranteed to arrive in perfect working condition
之后可以有技术支持。但是你自己最好懂小黑
state and zip:
PA 19122 |
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l***o 发帖数: 510 | 39 二手交易风险自负!请自行验证是否合法和一手卡!:
我想卖的物品:
Thinkpad X220 i5/IPS/2G/250G/蓝/指/摄 new@ $830
单张面值:
$830
可接受价格(必须明码标价!):
$830
物品新旧要求:
new,sealed in box
邮寄方式要求:
YL
买卖双方谁承担邮寄损失(Required if not code only):
Default
付款方式说明:
non cc Paypal
其他补充说明:
Condition New
Processor Intel® Core™ i5-2410M (2.30GHz base; 2.9GHz Max Turbo, 3MB Cache)
Operating System Genuine Windows 7 Home Premium 64 - Spanish
Keyboard/Pointing Device UltraNav (TrackPoint and TouchPad)
Total Memory 2GB PC3-10600 DDR3 1333MHz SDRAM SO... 阅读全帖 |
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i*******y 发帖数: 225 | 40 我想卖的物品:
X220 i7/IPS/4G/160gSSD/FP/BT/Camera/9 cell/6205
可接受价格(必须明码标价!):
$1300
物品新旧要求:
new, sealed
邮寄方式要求:
YL
买卖双方谁承担邮寄损失(Required if not code only):
before me after you
付款方式说明:
BOA,Chase,ING
其他补充说明:
0A69630 SBB INTCR I7-2620M PROC USB3.0
45M3092 VBB GENWIN7HOMEPREM64
0A59833 SBB GEN WIN7 HOME PRM64 EN
0A69642 SBB 12.5PRMHDLDBCLTDPYMBBBRDAN
45M4572 VBB 4GBPC3-10600DDR3 1333SODMM
45M4839 SBB KEYBOARDUS E... 阅读全帖 |
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j****u 发帖数: 3681 | 41 二手交易风险自负!请自行验证是否合法和一手卡!:
我想卖的物品:
Lenovo X220 X4
i7/IPS/4G/320G/Bluetooth/Fingerprint/Webcam
单张面值:
可接受价格(必须明码标价!):
$1050 X 4
Or pm your offer
物品新旧要求:
Brand new sealed
邮寄方式要求:
YL
买卖双方谁承担邮寄损失(Required if not code only):
before me after you
付款方式说明:
Billpay
广告的有效期:
till gone
我的联系方式:
PM
Warranty期限:
one year
state and zip:
39202
其他补充:
|
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y*****a 发帖数: 263 | 42 二手交易风险自负!请自行验证是否合法和一手卡!:
我想卖的物品:
Lenovo X220
i5-2520/IPS/2G/320G/Bluetooth&WIFI/WIN7 64
单张面值:
可接受价格(必须明码标价!):
$750
物品新旧要求:
Brand new, sealed
邮寄方式要求:
YL
买卖双方谁承担邮寄损失(Required if not code only):
before me after you
付款方式说明:
BOA
广告的有效期:
till gone
我的联系方式:
PM
Warranty期限:
one year
state and zip:
TN
其他补充: |
|
s*****g 发帖数: 5159 | 43 器材名称
HP ZR24w S-IPS Monitor
付款方式(需要其他付款方式请联系卖家)
BoA or Local cash
卖家联系方式
mitbbs mail
器材具体描述、价格、运费:
(均为现金/BOA/check/MO或non-cc paypal价,+$5 for echeck)
(其他付款方式引起的价格变动请与卖家联系)
Used, warranty to 04/2013, cosmetic 9+/10 condition, with all cables,
manuals, and original packaging with cushions. $300 + actual shipping.
Monitor located at Astoria, NY 11103 Pics: http://newyork.craigslist.org/que/sys/2455409667.html |
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w*****r 发帖数: 2540 | 44 想要的物品:
x220 i5 2520 ips
单张面值:
可接受的价格(必须明码标价!):
780 or depends
物品新旧要求:
现货
邮寄方式要求:
my label
买卖双方谁承担邮寄损失(Required if not code only):
default
付款方式说明:
alipay @6.48 or BOA
其他补充说明:
老id优先
广告的有效期:
物品来源:
我的联系方式:
二手交易风险自负!请自行验证是否合法和一手卡!: |
|
w*****r 发帖数: 2540 | 45 我想要的物品:
x220 i5 2410m//2g/250g/ips/蓝牙摄像头指纹/全新未开封!
单张面值:
780
可接受的价格(必须明码标价!):
780
物品新旧要求:
new
邮寄方式要求:
drop off Fedex
买卖双方谁承担邮寄损失(Required if not code only):
before u after me
付款方式说明:
alipay
其他补充说明:
广告的有效期:
till got
物品来源:
我的联系方式:
bbs
二手交易风险自负!请自行验证是否合法和一手卡!: |
|
d******i 发帖数: 1379 | 46 Thinkpad X220
单张面值: $1069
可接受的价格(必须明码标价!):
$1049
物品新旧:
NEW
配置:
Intel Core i7-2620M Processor
12.5" Premium HD (1366x768) LED Backlit Display (IPS), Mobile Broadband
Ready,2x2 Antenna
8 GB DDR3 - 1333MHz (2×4GB 联想原装ddr3内存)
320 GB Hard Disk Drive, 7200rpm
720万像素摄像镜头
Windows 7 professional 64-bit
(COA标签一般在背面放电池的位置)
厂家质保 到2012年8月
可以另送Win7安装光盘,重装可用背面COA的序列号
邮寄方式要求:
ML
买卖双方谁承担邮寄损失(Required if not code only):
Before me after you
付款方式说明:
non CC paypal
其他补充说明:
广告的有效期:
till gone
物品来源:
lenovo back to shool... 阅读全帖 |
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w*****r 发帖数: 2540 | 47 我想要的物品:
x220 ips i5 蓝牙摄像头指纹
单张面值:
可接受的价格(必须明码标价!):
less 800
物品新旧要求:
new
邮寄方式要求:
ml
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
boa
其他补充说明: |
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p*l 发帖数: 1658 | 48 我想卖的物品:
X220 i7/IPS/4G/320G/FP/BT/Camera/6205
可接受价格(必须明码标价!):
$975 or best offer
物品新旧要求:
new, sealed
邮寄方式要求:
YL
买卖双方谁承担邮寄损失(Required if not code only):
before me after you
付款方式说明:
BOA
Intel Core i7-2620M Processor (2.7GHz, 4MB L3, 1333MHz FSB) USB 3.01
12.5" Premium HD (1366x768) LED Backlit Display, Mobile Broadband Ready, 2x2
Antenna
4 GB DDR3 - 1333MHz (1 DIMM)
Fingerprint Reader +bluetooth
320 GB Hard Disk Drive, 7200rpm
720万像素摄像镜头 |
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s*****o 发帖数: 1565 | 49 Outlet弄的,本来打算自用,后来又不想要了
全新(不是refurbished),打开箱子检查了,无损坏,塑料包装未开封
保修到10/4/2012
$750,限Wisconsin地区当面交易
站内联系
Specification:
ThinkPad
X Series
X220 (latest model)
Black
Intel Core i5-2410M (2.30GHz base; 2.9GHz Max Turbo, 3MB Cache)
4GB PC3-10600 DDR3 1333MHz SDRAM SODIMM Memory (upgradable to 8GB with
additional $20)
320GB, 7200RPM Serial ATA 2.5" Hard Drive
12.5 HD WXGA (1366 X 768) IPS, LED
Intel HD Graphics 3000
Windows 7 Home Premium
Optical Not Included
9 Cell Lith... 阅读全帖 |
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x****n 发帖数: 176 | 50 我想卖的物品:
1台 X220T tablet 4296-2wu
配置:I5-2520/4G/320G 7200rpm/蓝/指/摄/多点触摸IPS/6Cell
3年质保到08/2014
自用机器,但是有了更好的,决定出手。
单张面值:
可接受价格(必须明码标价!):
1050
物品新旧要求:
开过箱子,但是一次也没有开过机,只是检查了一下部件。所有部件全新。
本来自用的,但是觉得太奢侈,决定转让。
邮寄方式要求:
Local or Your Label
买卖双方谁承担邮寄损失(Required if not code only):
付款方式:
boa
其他补充说明:
先款后货
广告的有效期:
Till Gone
物品来源(Required for All Cards!):
我的联系方式:
MITBBS站内PM
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
state and zip: |
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