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全部话题 - 话题: koh
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c*******4
发帖数: 7
1
一年4篇大作啊,好像postdoc才干了3年
让我们这样的千老情何以堪啊...
不懂这个领域,但还是想学习一下,看看怎么提高自己的效率,爬出这个坑
结果
我看了一眼,通篇的WB 和 chip 泡脚,昏过去了
有人读了这几篇Nature, Cell, Molecular Cell, NCB么?
能谈谈想法么?
ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes
the Warburg effect.
Yang W, Zheng Y, Xia Y, Ji H, Chen X, Guo F, Lyssiotis CA, Aldape K, Cantley
LC, Lu Z.
Nat Cell Biol. 2012 Nov 25. doi: 10.1038/ncb2629. [Epub ahead of print]
EGFR-Induced and PKCε Monoubiquitylation-Dependent NF-κB Activation
Upregulates PKM2 Expre... 阅读全帖
k*****n
发帖数: 323
2
我们用的是rick young 的protocol。
Lysis Buffer 1 Add protease inhibitors just before use, filter and keep cold
. Consists of 50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol,
0.5% NP-40, 0.25% Triton X-100, 1× protease inhibitors Lysis Buffer 2 Add
protease inhibitors just before use, filter and keep cold. Consists of 10 mM
Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1× protease
inhibitors
Lysis Buffer 3 Add protease inhibitors just before use, filter and keep cold
. Consists of... 阅读全帖
C***r
发帖数: 74
3
来自主题: ChemEng版 - 能在碱溶液中长久存在的polymer
谢谢! 我是想用来涂到样品表面,在80 C的KOH溶液中(44%)起保护作用.
polystyrene
polyisoprene,环氧树脂,尼龙,POLYIMIDE中有没有相对合适的?
m******e
发帖数: 432
4
没法附上附件,文献是 Journal of The Electrochemical Society, 153 (3) A468-
A471 (2006)
Bias-Assisted H2 Gas Generation in HCl and KOH Solutions Using n-Type GaN
Photoelectrode
d**a
发帖数: 3715
5
来自主题: Chemistry版 - 请问碱性洗液怎么配,包子。
以前配过, 用isopropanol, KOH, water,三者的大概比例忘了。谢谢
a********m
发帖数: 201
6
来自主题: Chemistry版 - 请问碱性洗液怎么配,包子。
好多种方法,一个人一个说法。不过我最近发现,用纯的isopropanol和KOH效果很好,
洗出来的瓶子残留少,也干净
b*****i
发帖数: 849
7
KOH+NaOH 1:1 mole mixture
L*****e
发帖数: 288
8
are you sure?
PhO- is leaving group?I doubt this~
Again, it is not necessary to use very strong base to form benzyne it depends on what is leaving group. sometime, KOH is enough!
http://www.chem.ucalgary.ca/courses/351/Carey/Ch23/ch23-5-1.html
p***e
发帖数: 402
9
来自主题: Chemistry版 - 不懂化学的请教一个化学反应...
ET_2IBr_2 + KOH 会有化学反应吗?最有可能会生成什么反应物?
还有, 如果用HF或者盐酸腐蚀 ET_2IBr_2, 两者会不会发生化学反应?
t******t
发帖数: 3045
10
溶液中的离子都是带水的,不是裸的啊
取决于你最后想要什么?
从KCl 到 KOH?
用离子交换柱把离子吸附了,再洗出来?
s*****n
发帖数: 60
11
做电化学实验测试碳在2M KCl的电容值,用碳作为工作电极,Pt作为负极,SCE (饱和
氯化钾)作为参比电极,电解液是2M KCl。循环伏安图出来就是一条直线。但换成其他
的电解液像Na2SO4,KOH等就没问题.是不是参比电极里的电解液和测试的电解液不能一
样?
谢谢!
d********w
发帖数: 1110
12
来自主题: Chemistry版 - 吡啶类化合物用盐酸洗入水相
用KOH试试?
s*******y
发帖数: 572
13
来自主题: Chemistry版 - 燃料电池的电极制备
文献中是这么介绍制备方法的:
2@C (45.0 mg) was introduced into a high-density polyethylene container (5
mL) together with water (1.0 mL), KOH (99.99% Sigma-Aldrich) (65.0 mg),
absolute ethanol (99.8% Fluka) (0.50 g) and 5% Nafion® ion-exchange
resin in alcohol solution (Sigma-Aldrich) (0.37 g). The resulting suspension
was sonicated for 30 min with a Branson 3200 bath. The suspension was
always freshly prepared. The metal loading on glassy carbon electrode was
determined by weighting the amount of ink de... 阅读全帖
j****C
发帖数: 72
14
来自主题: Chemistry版 - 燃料电池的电极制备
I don't know where you got this reference,honestly I cannot understand what
you are doing. In yours citation Nafion is used and therefore you are
working PEM fuel cell(?), but Idon't know why you add the ink with some KOH
which will have chance to covert H-Nafion to Na-Nafion without proton
conductivity. At fuel cell industrials, CCM is standard electrode
preparation method.
s*******y
发帖数: 572
15
来自主题: Chemistry版 - 燃料电池的电极制备
谢谢回复!
这段文字出自这篇文献里的SI:
http://onlinelibrary.wiley.com/doi/10.1002/anie.201002234/abstr
KOH是用来转化催化剂的,用的量也很小。
我不是做这一行的,就是比较感兴趣。所以问问。能大致介绍一下CCM是怎么制备的吗
s*******y
发帖数: 572
16
来自主题: Chemistry版 - 燃料电池的电极制备
文献中是这么介绍制备方法的:
2@C (45.0 mg) was introduced into a high-density polyethylene container (5
mL) together with water (1.0 mL), KOH (99.99% Sigma-Aldrich) (65.0 mg),
absolute ethanol (99.8% Fluka) (0.50 g) and 5% Nafion® ion-exchange
resin in alcohol solution (Sigma-Aldrich) (0.37 g). The resulting suspension
was sonicated for 30 min with a Branson 3200 bath. The suspension was
always freshly prepared. The metal loading on glassy carbon electrode was
determined by weighting the amount of ink de... 阅读全帖
j****C
发帖数: 72
17
来自主题: Chemistry版 - 燃料电池的电极制备
I don't know where you got this reference,honestly I cannot understand what
you are doing. In yours citation Nafion is used and therefore you are
working PEM fuel cell(?), but Idon't know why you add the ink with some KOH
which will have chance to covert H-Nafion to Na-Nafion without proton
conductivity. At fuel cell industrials, CCM is standard electrode
preparation method.
s*******y
发帖数: 572
18
来自主题: Chemistry版 - 燃料电池的电极制备
谢谢回复!
这段文字出自这篇文献里的SI:
http://onlinelibrary.wiley.com/doi/10.1002/anie.201002234/abstr
KOH是用来转化催化剂的,用的量也很小。
我不是做这一行的,就是比较感兴趣。所以问问。能大致介绍一下CCM是怎么制备的吗
a*******i
发帖数: 11664
19
你列的几片paper,Peidong Yang, Yi Cui都是Charles Lieber的学生,在业界也算是
学霸了吧。nanoletter就是他们家开的。
你说的液体电极那片是Atwater他们组发在Nauture Mat上的那片?
发Paper第一看老板的背景,第二看idea够不够fancy,novel。至于有没有使用价值,
不是发paper应该考虑的。
KOH etch Si现在来看是最便宜效果最好的东西。但是只能用在(100)的单晶硅上,对多
晶硅和a-Si都不适用。
如果你做surface texturing的方法是创新的,并且效果比较好,发nano letter有希望
,但前提条件是你的方法别人没用过。
n****k
发帖数: 11
20
来自主题: Environmental版 - 水处理问题,请赐教
各位好,
我们家的井水需要提高pH值,处理后供全家日常生活使用。我们附近的水处理公司只提
供弱碱如Soda Ash,但家里现有的Feeding Pump速度不够高,所以不能采用,除非重装
一套系统。或者只能用他们的Service,超贵。
现在我希望自己能买强碱,如氢氧化钾或者氢氧化钠(KOH或者NaOH)。我们附近的水
处理公司都不愿意直接卖强碱给我们。在网上看到两个地方有卖,标明Food Grade,不
过都没有明确标明可以用于水处理。因为不在这个行业,不知道Food Grade的标准比水
处理要求的标准高还是低。我们可以买他们的产品吗?
http://www.aaa-chemicals.com/sodium-hydroxide.html
http://www.dudadiesel.com/search.php?query=Potassium+Hydroxide
http://www.dudadiesel.com/search.php?query=sodium+hydroxide
如果都可以的话,有推荐吗?
先谢谢回复!
h*****1
发帖数: 26
21
来自主题: Macromolecules版 - 有机合成试验 求助
有机合成试验:N烃化反应,用甲醇作溶剂,反应过程中有盐酸产生,为了综合盐酸,
我一开始用KOH,但是副产太多,近期我用碳酸钾或碳酸钠代替氢氧化钾,副产少了,
但是拿出来得产物气象色谱出峰时间不同,而且外观明显不同,这个产品是手性的,但
是反应位置不涉及手性碳分子,请高手指点我的实验变化会不会使产品消旋,期待着你
们好的建议,非常感谢!
M***7
发帖数: 388
22
Thanks for your response, I appreciate it!
Actually, the Melamine-Urea-Formaldehyde(MUF) is the shell material of a
type of microcapsule encapsulating some special core materials.We want to
use these microcapsules with different core materials in a high-alkali
environment such as KOH,NaOH or Ca(OH)2 solution with a pH aroud 12 at 70-
80C degrees. Certainly, we would like to see these microcapsules with
Melamine-Urea-Formaldehyde(MUF) shell could remain stable in high-alkali
environment as long a
c*s
发帖数: 2145
23
来自主题: Macromolecules版 - 30个包子问问题
文献里有酸性和碱性水解,碱性是KOH/MEOH,酸性是HCL/MEOH/H2O
e****0
发帖数: 678
24
来自主题: MedicalCareer版 - [我的CK笔记]妇产科
 Screening
 Pap smear for vervical cancer is 21 yo
 DM
24-28 weeks
One hour 50 grams oral glucose tolerance test. BG > 140
Three hours glucose tolerance test (if two or more are positive)
1. Fasting BG > 95
2. One hour BG > 180
3. Two hours BG> 155
4. Three hours BG > 140
• Dysfunctional uterine bleeding (DUB)
 Heavy vaginal bleeding occurs in the absence of structural or
organic disease
 Endometrial biop... 阅读全帖
d******u
发帖数: 47
25
来自主题: MedicalCareer版 - step3 Archer 3题求助 谢谢!
1, Your patient is a gravida 2 para 2 female with a complaint of a vaginal
discharge, vulvar irritation, and dysuria. Her symptoms have recurred 4
times within the past year. She had a recent urinary tract infection that
was treated with ciprofloxacin, but is in otherwise good health. She is
afebrile with normal vital signs, and pelvic examination reveals vulvar and
vaginal erythema with a thick white discharge in the vaginal vault. KOH prep
reveals pseudo hyphae. Which of the following treatmen... 阅读全帖
C***r
发帖数: 74
26
好像positive resist和PMMA都在加热的KOH中呆不到几个小时,不知道有没有能呆久一
点的,any suggestion is welcome
m********t
发帖数: 22
27
如果要求不高,把热蜡油涂到表面,凝固后可以防止KOH腐蚀。有专门除蜡的试剂,叫
Opticlear,也可以用类似苯的试剂除掉。
z******n
发帖数: 494
h*****1
发帖数: 26
29
来自主题: Pharmaceutical版 - 有机合成试验求助!!!
有机合成试验:N烃化反应,用甲醇作溶剂,反应过程中有盐酸产生,为了综合盐酸,
我一开始用KOH,但是副产太多,近期我用碳酸钾或碳酸钠代替氢氧化钾,副产少了,
但是拿出来得产物气象色谱出峰时间不同,而且外观明显不同,这个产品是手性的,但
是反应位置不涉及手性碳分子,请高手指点我的实验变化会不会使产品消旋,期待着你
们好的建议,非常感谢!
t********y
发帖数: 1806
30
来自主题: WBCenter版 - 我爱宝宝版申请代发求祝福包子
Thanks a lot!

求祝福
benbenzhu, cautree, ccyang59,
cerealmilk, changchang, cline, cuddlebear66, DaMaoDing, daxue, delia78, dfef
, didiwo, doglovers,
DreamChaser9, DreamerOnAir, driving, dttfish, elena83, en0808, erjie,
ersiezh, floranumen, flyfish00,
frankiezhao, Gary2008, Goodgood2010, guagua1, gzzhang917, hannah1209,
HappyF1, HeGuaMei, heidi,
Hemerocallis, hoohoo001, Jessicamm, jing2009, jjneimei, johnpony, jwmm,
Kaka22, kieky, KOH, lazycats,
lee: an12012, lenamama, LGeGe, lilypad, lmfe, LOVELYB
m*********k
发帖数: 10521
31
来自主题: WBCenter版 - Military版申请代发包子
"[Military]TempBM Oct 31. ● (过十万,再接再励!)众志成城,白宫请愿
签名需要你"
成功奖励 50 伪币的用户: CATCAT, fourriver, ptx, Doser, IV, rebecca1975, PT,
huyanke, allence, ilovqianqian, AnyMoreLeft, laoshu09, hahaha2012, hj630,
TempBM, happymyt, OldLady80, shinn, davidcrazy, cathy1061, MMT007, shenms,
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juliemzhu, iruka, xiaose, rooster, acespade, crasher, mit09, xinchong,
jonsun, hyp... 阅读全帖
m*********k
发帖数: 10521
32
来自主题: WBCenter版 - 探亲/陪读版代发包子申请
"[Reunion]qiaqiafeng Sep 5 ● 中秋包子贴"
成功奖励 20 伪币的用户: XjtuGordon, wgyeric, voyiger, utkfan2013, toughgrass, Tomato9, tinyyao, tankengine, swingtrader, SushiRoll, sayusaymi, Rosefield, qma, qinshuiyuan, qiaqiafeng, purzel, pumpkin18, Pumbaa, PandaAlpha, oxwah, Noktard, lovefreedom, littlesucker, KOH, katie83, julietlyzhu, jocelynxu, jcai, hulu22, Happier926, firefox77, FinalSale, chunmian, boysd, BNS, angelicafj
扣除版面:(Reunion)720伪币成功
G***S
发帖数: 378
33
发信人: GSAKS (Koh), 信区: WashingtonDC
标 题: Re: 请推荐钢琴老师 (20850), 多谢!
发信站: BBS 未名空间站 (Fri Dec 27 16:31:59 2013, 美东)
你可以試一試270 (11號出口)這位女老師,有責任心,也有愛心與耐心,和孩子非常
投緣,
比较注重培養孩子的學習興趣,收費還挺合理的
朱老師
240-404-8208
Y*********[email protected]
s******v
发帖数: 477
34
来自主题: Medicalpractice版 - had a class today "Dermatology for the internist"
Had the class today. I'd like to share with you the top ten list of DO's and
DON'Ts. I may update later when I have more time.
DO...
1.Know how to properly decribe skin lesions and eruptions
2.Pick faorite generic low, medium and high potency topical steroids for
appropriate usage
3.Understand how to prescibe topical retinoids
4.Recommend sun protection to all rosacea patients, and educate on other
triggers
5.Reommend a consistent home therapy regimen to all wart patients
6. Discuss daily skin c... 阅读全帖
w*******y
发帖数: 60932
35
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$14
Aviva Comfy Lounger:
http://www.kohls.com/koh
m*********k
发帖数: 10521
36
来自主题: _mitbbscheck版 - 7月2日 文章奖励
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