t****g
发帖数: 35582 | 1 这我倒是碰了不少。当年FAR的盘好多第二天就读不出来的。 |
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G**Y
发帖数: 33224 | 2 我有很多CD-R读不出来了。主要是物理损伤，那些盘很容易掉渣。
所以现在我基本不用光盘存东西了。 |
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t****g
发帖数: 35582 | 3 唉，可怜的Sony。花了大价钱还容易把HD-DVD给整死了，没想到自己也被云彩给吞了。
我现在装机连光驱都懒得配了。家里扔一个外置的USB光驱，偶尔非需要的不可的时候
拿出来用用。 |
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G**Y
发帖数: 33224 | 4 呵呵，same here，
我也没光驱，不过偶尔还是想用一下，
当时为了省钱，
买的那个外置光驱特别巨大。
搞得我都懒的使，
应该买个usb power的就好了。 |
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k*****n
发帖数: 9823 | 5 Deal时25刀一个
我是旧笔记本上的拆下来，Ebay上买了个USB盒子 |
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i**i
发帖数: 1500 | 7 http://www.infoworld.com/print/236070
Meet AOSP, the other Android, while you still can
By Galen M. Gruman
Created 2014-02-14 04:00AM
No doubt you've seen the stat showing how much more popular Android
smartphones are than iPhones in much of the world. But many of those Android
smartphones aren't the Android you're thinking of -- the kind you'd get
from Samsung, HTC, or Motorola. That's because there's more than one Android
. In fact, some analysts believe that about half of the Android devices ... 阅读全帖 |
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n*******p
发帖数: 231 | 8 You want to be careful also because system analyst is a career lack of
longevity and often dead end...
Company buys a system with millions of dollars and often ended up keeping it
for long time. As a system analyst in-house, you often ended up supporting
an old system and all the obsolete knowledge you accumulated over the years
will do you no good when you realize you want to change jobs...you ended up
competing with all the young folks...it is sad...
Where I am, I see a lot of students craze a... 阅读全帖 |
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z********c
发帖数: 1268 | 9 我觉得life insurance都没什么动静，是不是annuity关注更多一些？还是这就完全是
一帮爱做research的actuaries在自说自话，公司根本不care？ |
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z********c
发帖数: 1268 | 10 我觉得life insurance都没什么动静，是不是annuity关注更多一些？还是这就完全是
一帮爱做research的actuaries在自说自话，公司根本不care？ |
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s*********s
发帖数: 110 | 12 Xiaodong Wang
Chen She(Mass Spec Core Director)
Xiaochen Wang
Mengqiu Dong(外号董姐姐,做线虫,longevity,驻颜有术)
这位被挡住了,据我估计是Chai Jijie
Xiaoguang Lei(Chemistry Core Director) |
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s******d
发帖数: 303 | 13 有些假阳性是因为data cleaning 没做好，genotyping error 造成的。比如最近闹得
沸沸扬扬
的longevity GWAS 发在science 上。就像一个靶子一样被一堆人披了。
GWAS 本身从方法上来说是不错的，但是数据是人收集的，难免有mis-classificaiton,
mis-
identification of samples. 还有很多其他的遗传背景，认为数据采集，以及一些环
境干扰因
素，影响了power。况且GWAS的假设就是common variant， which means MAF>10%. 另外
common disease model， architecture, 等这些都是结果比大家期望的低。
前一段时间nature genetics 上一个关于升高的GWAS, 用所有GWAS的marker 来计算好
像整个
autosomes account for ~40% Height variance. |
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a********k
发帖数: 2273 | 14 花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branches in lamellipodia. Cell. 2008 Sep
5;134(5):828-42. PubMed PMID: 18775315; PubMed Central PMCID: PMC2570342.
Cai L, Makhov AM, Bear JE. F-actin... 阅读全帖 |
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l********g
发帖数: 19 | 15 自己顶一个。
主要觉得在座的诸位从事基础研究的多些，而且蛋白折叠现在还是热门，跟什么
proteostasis，longevity，mitochondria function，neurodegenerative disease等
等都有关系，怎么没人为这个激冻一把呢？ |
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y******8
发帖数: 1764 | 16 The whole Sir2-aging story began with the focus on a single gene. In
contrast, Insulin/IGF-longevity research is based on non-biased genetic
screens.
I would like to say that the phenotype-to-genetics-to-mechanism strategy
might be difficult but can hardly go wrong. You have to be a genius to start
from the a single protein to figure out the function at organism level,
even with the help of advanced structure biology. |
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h*******o
发帖数: 4884 | 17 That's even more complicated if you bring evolution in.
Female reproductive senescence starts at late 40s to early 50s, which means
that once over 55, female is of no reproductive advantage or usage to the species, therefore a pro-longevity gene/phenotype that only offers mild advantage in female at over 55 years or older should not be considered as a result of evolution.
in |
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y******8
发帖数: 1764 | 18 So, you are talking about the human aging. Well, evolution might not have
direct selection on post-reproductive stage, but it does on reproductive-
span for sure. In fact, women with delayed menopause have longer lifespan
expectation.
Actually, most animals at wild die long before they reach the beginning of
their reproductive senescence.
means
species, therefore a pro-longevity gene/phenotype that only offers mild
advantage in female at over 55 years or older should not be considered as
a re... 阅读全帖 |
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y******8
发帖数: 1764 | 19 I think it also true for most mammals, including small, middle and even some
big-sized ones.
IGF-1 knockdown surely impairs fertility. In theory, if the animal gets
endocrine regulation on IGF-1 production in response to harsh environment,
the animal would halt the reproductive activity. When condition improved,
the IGF-1 level would go back and the reproduction can be continued. Thus,
the energy and effort would not be wasted because raising infants would be
much more difficult than maintaining... 阅读全帖 |
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y******8
发帖数: 1764 | 20 So far, no experimental aging research has gone beyond the frame Kenyon (
probably Ruvkun also) drew almost a decade ago. She is vigorously trying to
prove a unique aging pathway (unlike Ruvkun at this point). To summarize,
the aging regulators are Insulin/IGF pathway + reproductive system + subsets
of neurons in the CNS(other discoveries are just modulators). Unfortunately
, the longevity regulation of all these are non-separable from other more
profound effects.
The aging theory itself has evo... 阅读全帖 |
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L*******e
发帖数: 2153 | 21 怎么讨论calories restriction and longevity讨论到菜系去了。哈哈～ |
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h*****n
发帖数: 924 | 22 You sounds awfully like a lame student. Or should I say you reek of a lame s
tudent.
the reek is not whether you are a lame student, but the fact that you try to
justify lameness by developing an illogic theory (and feeling good about it
).
nobody says hardworking is necessary for success. is non smoking a necessary
condition for longevity? should people smoke?
you set a fake enemy and shoot it down. that is pathetic, even by the mitbbs
standard. |
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k*****1
发帖数: 454 | 23 恩，包括子刊。
20.
Resequencing 50 accessions of cultivated and wild rice yields markers for
identifying agronomically important genes.
Nature Biotechnology. Epub 11 Dec 2011
19.
Frequent mutations of genes encoding ubiquitin-mediated proteolysis pathway
components in clear cell renal cell carcinoma.
Nature Genetics. Epub 04 Dec 2011
17.
Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of
resource-poor farmers.
Nature Biotechnology. Epub 06 November 2011
16.
Ascaris suum draf... 阅读全帖 |
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b******y
发帖数: 627 | 24 longevity + a little bias at birth. |
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D*a
发帖数: 6830 | 25 Nature忘了它有一整本杂志都是这玩意儿了？
两年前Science发了一篇GWAS方法研究人类长寿相关基因分析的文章，一年后被说有技
术问题，假阳性，撤稿了，最近刚从Plos One发出来了。两年前就瞅了一眼结果，现在
看貌似大结论没变啊？还没细看，细看也看不懂。。。
http://www.nature.com/nature/journal/v487/n7408/full/487427a.ht
Scientists and journals must work together to ensure that eye-catching
artefacts are not trumpeted as genomic insights, says Daniel MacArthur.
When a study of the genomes of centenarians reported genetic variants
strongly associated with exceptional longevity1, it received widespread
media and publi... 阅读全帖 |
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c*******0
发帖数: 190 | 26 顶一下...在不考虑外界理化因素影响（辐射、活性氧、外毒素等对细胞的损害）的情
况下，端粒/端粒酶系统确实是已知的最主要的控制细胞寿命的生理机制。这个领域应
该至少还会有一个炸药奖吧。
说起older father，之前人们普遍认为年纪大的父亲会将更多的genetic mutation传给
子代，因此应该尽早生育；不过，看看今年6月发表在science上的一篇文章：Children
of older fathers 'are more likely to live longer'...因为子代有更大的概率遗
传到更长的端粒...So，长端粒是否可以视为一种进化？
大部分癌症（80%，某文献报导）拥有异常升高的端粒酶活性，基于此，是否可以将其
视为一种进化 in terms of longevity？
但不要忽略那20%，其中一个典型是IVS型的neuroblastoma，基本没有端粒酶活性，但
却是高度恶性肿瘤。
对现时的生物学，太多的悖论，太多的矛盾，太多的junk文献，认真你就输了。 |
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D*a
发帖数: 6830 | 32 相关的2篇
Biol Reprod. 2011 Mar;84(3):613-8. doi: 10.1095/biolreprod.110.088831. Epub
2010 Dec 8.
Generation of viable male and female mice from two fathers.
Deng JM, Satoh K, Wang H, Chang H, Zhang Z, Stewart MD, Cooney AJ, Behringer
RR.
Longevity in mice without a father.
Kawahara M, Kono T.
Hum Reprod. 2010 Feb;25(2):457-61. doi: 10.1093/humrep/dep400. Epub 2009 Dec
1.
（data略坑）
第二个问题，染色体可以互相融合，或者扯断，这种情况下染色体数目就不一样了。
看这个图
http://www.nature.com/scitable/content/the-genetic-similarity-o |
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D*a
发帖数: 6830 | 33 相关的2篇
Biol Reprod. 2011 Mar;84(3):613-8. doi: 10.1095/biolreprod.110.088831. Epub
2010 Dec 8.
Generation of viable male and female mice from two fathers.
Deng JM, Satoh K, Wang H, Chang H, Zhang Z, Stewart MD, Cooney AJ, Behringer
RR.
Longevity in mice without a father.
Kawahara M, Kono T.
Hum Reprod. 2010 Feb;25(2):457-61. doi: 10.1093/humrep/dep400. Epub 2009 Dec
1.
（data略坑）
第二个问题，染色体可以互相融合，或者扯断，这种情况下染色体数目就不一样了。
看这个图
http://www.nature.com/scitable/content/the-genetic-similarity-o |
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c********r
发帖数: 1125 | 34 跑胶间隙，神棍预测一把：
概率高的：
leptin （Friedman/colman)
nuclear receptor (jensen/chambon/evans)
这两组里面都有岁数很大的哥们了，再不给要死了就不好给了。
概率中等的：
chaperone
motor protein
miRNA(毕竟RNAi 刚给过）
神经领域（节律，触觉...)，好多年没给了..
概率小的
longevity
SNAREs
autophagy
PALM/STORM/STED |
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D*a
发帖数: 6830 | 35 pubmed reproduction longevity |
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D*a
发帖数: 6830 | 36 “老鼠在这个基因敲除后，端粒长度没有变化，没有其他任何表型。”
“老鼠端粒长度突然短”
你在逗我？？
http://www.cell.com/cell/abstract/S0092-8674%2801%2980006-4
摘要：mTR-/- mice lacked detectable telomerase activity yet were viable for
the six generations analyzed. ...Telomeres were shown to shorten at a rate
of 4.8 +/- 2.4 kb per mTR-/- generation. Cells from the fourth mTR-/-
generation onward pos- sessed chromosome ends lacking detectable telo- mere
repeats, aneuploidy, and chromosomal abnor- malities, including end-to-end
fusions.
这是最原始的文献，
下... 阅读全帖 |
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m******g
发帖数: 467 | 37 PNAS 15.01.20
小知识：
1. Uncoupling lifespan and healthspan in Caenorhabditis elegans longevity
mutants |
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c*w
发帖数: 10 | 40 Monday, January 12 and Tuesday, January 13 - Andrea Pauli, Harvard
University
Seminar on Monday, January 12 at 10:00 a.m., Koch Auditorium, Rm. 76-156; "
From genome to gene function"
(Candidate for the Koch/MIT Biology Search)
=====
Tuesday, January 20 and Wednesday, January 21 - Steve Brohawn, The
Rockefeller University
Seminar on Tuesday, January 20 at 10:00 a.m., McGovern Auditorium at the
Whitehead Institute; "How do we feel? Physical mechanism for gating and
mechanosensitivity of the huma... 阅读全帖 |
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c*w
发帖数: 10 | 41 Monday, January 12 and Tuesday, January 13 - Andrea Pauli, Harvard
University
Seminar on Monday, January 12 at 10:00 a.m., Koch Auditorium, Rm. 76-156; "
From genome to gene function"
(Candidate for the Koch/MIT Biology Search)
=====
Tuesday, January 20 and Wednesday, January 21 - Steve Brohawn, The
Rockefeller University
Seminar on Tuesday, January 20 at 10:00 a.m., McGovern Auditorium at the
Whitehead Institute; "How do we feel? Physical mechanism for gating and
mechanosensitivity of the huma... 阅读全帖 |
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c********r
发帖数: 1125 | 42
听过不止他一次seminar，跟他也在会议上聊过.
对他印象非常不好，诚然他人很聪明，很会做seminar，但是很多他的研究就是看起来
非常fancy的idea，支持的数据非常weak...他在我们这做讲座data就被一个大牛质疑过
，最后以他巧妙的四两拨千斤的以一堆问题回答了一个他回答不了的问题结束...
他做的这个亚领域很多数据重复性非常差，虽然很多生物实验的数据都会有误差，但是
线虫longevity和代谢很多有名landmark paper数据都非常没有重复性，我原来楼上实
验室就有2篇CNS发在这个领域..，在我们联合组会被修理的体无完肤...但是，照样发
CNS.
现在线虫领域就是几个大牛把持话语权，灌水吃肉，剩下人喝汤都困难，国内做线虫的
经费很难拿到，几乎做的好一点的都去忽悠老鼠的经费去了...实话说很多大牛的文章
质量非常差..完全就是贩卖概念...
我劝你除非能进那几个学霸实验室，否则离线虫领域远一点... |
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