O******e
发帖数: 4845 | 1 ☆─────────────────────────────────────☆
idiotic (confused) 于 (Fri Jan 19 21:48:19 2007) 提到:
For pioneering biochemical studies on apoptosis, which have elucidated a
molecular pathway leading into
and out of the mitochondrion and to the nucleus.
Former winners include Brown and Goldstein, Alfred Gilman, Gunter Blobel,
Richard Axel, Stanley Prusiner,
John Kuriyan...
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forfun2060 (悠悠观天下) 于 (Sat Jan 20 00:34:17 2007) 提到:
值得骄傲!会有越来越多的华人在这个领域里作出惊人的成绩来的, |
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l*********i
发帖数: 332 | 2 版主好勤劳阿！！
还没等我解说一把。此贴天南海北扯了不少，本人感觉内容还是挺多，想稍稍总结一下。
从此贴楼主的连接，我去看了一下，美国科学院这个奖确实份量很重，是奖励各个领域
重大发现的。楼主也列出了
几个获得此奖的Nobel奖得主。
我在看Axel的过程中发现，axel得奖是93年，那个时候他刚刚发现嗅觉受体家族，对整
个嗅觉系统的组织还没有全
力投入，大家如果看过Axel获奖演说的话就会明白，Axel原来是跟嗅觉研究8杆子打不
找的，他就喜欢克隆基因，
克隆了无数的基因，比如CD4（好像，懒得查了，如果错了，抱歉），等等。而04年他
和Buck获奖的理由是，"for
their discoveries of odorant receptors and the organization of the olfactory
system"。 可见，光发现一个
超级基因家族，而不对其功能进行深入的研究，是没戏拿炸药奖的，至少没那么快，如
果Axel自从发现嗅觉基因家
族后，再也不碰，充其量到他80岁的时候，跟别人share一个关于嗅觉的炸药奖。
而我认为之所以他可以那么快的拿到，是因为他当 |
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p*****m
发帖数: 7030 | 3 你又错了 你说的和heculase说的八成不是一回事。生化纯化的过程都需要assay 否
则他们纯化什么？而且一般大家都希望简单方便的ASSAY 这样可以节省很多时间 而
且最好这个assay就是all-or-none,方便指导纯化。至于说纯化出来的东西怎么做更
细致的assay去分析功能，那是另一个问题，而且我个人意见这比设计一个简洁高效
的纯化assay容易多了。
比如说吧，检测apoptosis的assay太多了 从形态到细胞学到生化方法，但是XD纯化
cytochrome C的时候用的assay很简单，就是看紫外照射以后那个细胞组分能够激活
caspase的活性。事前来看当然问题多了，谁说caspase的活性就一定是apoptosis的
指标了？这个assay压根就不能反映apoptosis的复杂机制。但是无论如何人家拿到
有意义的蛋白了，后面的问题都不存在。
说这个去加基化酶，如果是我来做这个实验的话，我用的assay肯定非常简单，比如
说，Gurdon的paper提到几个干细胞marker的启动子区会被甲基化/去甲基化 那么最
简单的assay就是purify能够去甲基化这 |
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r****r
发帖数: 379 | 4 我很奇怪为什么连fly的凋亡都没有mitochondria pathway,而且mitochondria
pathway在体内的生理和病理情况下作用是否如culture cells里显示的那样重要
还很难说,比如bid,05年发现可以在核内起作用,有理由相信以后会有更多类似的发现.
所以,在更多的in vivo data出来之前,我对mitochondria pathway的重要性还带着疑问.
不过,尽管如此,我完全同意你的看法,XD的工作还是非常非常重要的,得不得nobel
prize已经显得不那么重要了. 这年头没得nobel prize的重要工作海了去了,得了
却不怎么让人觉得重要的东西也不少,前有mRNA的发现,后有EGF的发现.
Not sure I got ur points fully:))). But As far as I am thinking, there is
virtually no doubt whoever discover the mysteric enzyme(family?) will be a
very competitive contender |
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n********k
发帖数: 2818 | 5 you could well be right. But the importance is way different. Just as Andrew
fire and mello won Nobel prize not Greg Hannon and etc etc, right? Why, for
anything important like this, it is more often than not no single people
can figure all its functions but it only takes one to discover the gene/
phenoma, right? Indeed it is expected that the functional part of
demethylation/methylations is way more complicated than several assays and
people have been studying the functions for decades and it m |
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n********k
发帖数: 2818 | 6 Yes, you are right, that's why I say specifically mammal(BTW, need to say
upfront, I am much less familar with non-verterbrate systems). Additionally,
although it doesn't seem Mitochondrial plays a central role but that doesn'
t deny MT pathway cause it is also called intrinsic pathway. Many players
are still there but may not reside in MT. And some people even suggest maybe
in fly, you don't need to cytochrome C release from MT cause it can
initiate the death pathway right at MT.
Regarding the |
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r****r
发帖数: 379 | 7 我完全同意你的看法. MT的作用,应该不限于release pro-apoptotic factors,XD的主要
贡献在于release pathway的发现,当然还有extrinsic和intrinsic pathway之间的
crosstalk,不过我更欣赏MT作为一个unit在apoptosis中的作用.这样来说的话,release
factors只是
其中一种作用方式,XD如果当时接着去挖掘更多的MT作用,可能地位还会更高.
我记得他说过他当年思考过为什么进化会选择MT来调控apoptosis,可惜当时人们对MT
在apoptosis中的作用估计的太简单了,以为XD的工作一出来,就差不多搞定了,XD自己
估计也是这么认为,从inverterbrate animals来看,既然这些物种里面MT上很多蛋白在
不需要cytoc的情况下就可以调控apoptosis,那么有理由相信mammalian system里面这
些pathway
也会很重要,如果XD当时把这个也一举搞定,那就太牛了.
现在有些晚了,概念已经有了,剩下的就是细枝末节了.
Additionally,
doesn' |
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n********k
发帖数: 2818 | 8 Yes, you definitely see the deepside of the whole story. I am sure XD Wang
tried that and he indeed have done more than releasing factors. In some
senses, His chance for Nobel was more based on MT functions. However,
remember MT is just too complicated, virtually now everything involves MT.
MT biology is just way too complicated and it is everywhere as engine and
redox producing center, and meabolic center.....,I guess he must have tried
but there are things beyond his lab expertise. To stay cl |
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p*****n
发帖数: 981 | 9 ☆─────────────────────────────────────☆
Oncogene (基因头) 于 (Tue Jan 30 00:25:07 2007) 提到:
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idiotic (confused) 于 (Fri Jan 19 21:48:19 2007) 提到:
For pioneering biochemical studies on apoptosis, which have elucidated a
molecular pathway leading into
and out of the mitochondrion and to the nucleus.
Former winners include Brown and Goldstein, Alfred Gilman, Gunter Blobel,
Richard Axel, Stanley Prusiner,
John Kuriyan...
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s********6
发帖数: 89 | 10 http://www.amc.edu/PR/PressRelease/04_20_15_drs.html
http://www
Albany Med Announces 2015 Recipients of Albany Prize in Medicine
Albany, N.Y., April 20, 2015—A pair of scientist/inventors who developed
widely used modern research technologies that promise to accelerate medical
discoveries have been announced as the recipients of the Albany Medical
Center Prize in Biomedicine and Biomedical Research for 2015. They are:
Karl Deisseroth, M.D., Ph.D., D.H. Chen Professor, Professor of
Bioengineering... 阅读全帖 |
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