J*****3
发帖数: 4298 | 1 纪念查尔斯•达尔文诞辰200周年
作者简介：龙漫远，美国芝加哥大学生态与演化学系终身教授，北京大学生物信息中心
长江学者讲座教授，主要从事遗传学与演化论研究；陈振夏，北京大学生物信息中心博
士研究生, 主要研究兴趣是基因演化和生物信息学。
一千年前的中原，凡有水井处，皆唱柳词；今天的世界，凡有科学之处，皆说达尔文。
http://www.plob.org/2011/12/14/971.html
一 超越时空界限与学科藩篱
1809年2月12日，查尔斯·达尔文（Charles Robert Darwin）出生在英国什罗普郡的历
史名城什鲁斯伯里（Shrewsbury）。1859年11月24日，他的不朽名著《物种起源》出版
，一时洛阳纸贵而影响历久不衰。从他的家乡到世界各地，不同信仰的人们或欢呼，或
沉思，或惊愕，或暴怒，谈论的中心议题就是以自然选择理论为基础的演化学说（
Evolution based on the Theory of Natural Selection）。200年后的今天，世界上
许多国家和地区的学者和民众都投入了纪念这位科学伟人的盛事：英国、法国、德国、
... 阅读全帖 |
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w***e
发帖数: 1331 | 2 不过你很可疑欧。 很久没有露头了，突然间几篇帖子不见了，你就出来了。嘿嘿。
这样吧， 为了洗脱你的嫌疑，帮我找找这篇文献
Colloid & Polymer Science
Issue: Volume 275, Number 7
Date: August 1997
Pages: 640 - 647
A mechanistic study of the formation of polymer nanoparticles by the
emulsification-diffusion technique |
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g****y
发帖数: 26 | 3 We are seeking highly motivated individuals for postdoctoral positions in
the Structural Biology Program of the Sloan-Kettering Institute, Memorial
Sloan-Kettering Cancer Center, NYC, USA.
The lab develops cutting-edge techniques (Nature,doi:10.1038/ nature09163 (
2010)) aimed at gaining a mechanistic understanding of fundamental
biological processes. Major emphasis is placed on development of novel
single-molecule detection and manipulation instrumentation that pushes the
envelop in sensitivity... 阅读全帖 |
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F****n
发帖数: 3271 | 4 If he is the author of javac, then he is a C++ programmer. He may know Java's syntax and JVM mechanism well, but not necessary the language design. Other people designed Java and he is just an implementer. Just like a garage mechanist who knows Mercedez well do not necessarily know how to design a Mercedez and why it is designed that way. |
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F****n
发帖数: 3271 | 5 You can even go ahead to argue that the mess he / others put in Java
Generics and Scala is a good example of what will happen when a garage man
think he can design a car better than Mercedez. That's a little mean but I
believe a lot of people would agree:)
Java's syntax and JVM mechanism well, but not necessary the language design.
Other people designed Java and he is just an implementer. Just like a
garage mechanist who knows Mercedez well do not necessarily know how to
design a Mercedez and w |
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p*****m
发帖数: 7030 | 6 I see 鱼里面一个position cloning要多久呢 俺觉得让新学生去做这个有点不人道 赫
赫 风险还是蛮大的说
我觉得给新生的东西应该不涉及mechanistic insight 比如设计个assay 描述个现象之
类的 能发个10分以下的小杂志就行 然后可以让他们搞个大的出来 |
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n********k
发帖数: 2818 | 7 don't know, I haven't followed closely lately with this as I do mostly in
vivo now...in vitro is just for confirmation or mechanistic probing...for ur
case, it is likely less a problem...I am more talking about in term of
phenotypical changes...BTW, as I said earlier, I don't see why you have to
use stable cell lines if with retroviral... |
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R**********n
发帖数: 473 | 8 我朋友正为上海联合利华招聘美国博士（后）毕业生人才。
The following is the job description.
Description of main responsibilities
•Build up the toolbox mainly in vitro to identify actives with
cardiovascular benefits for functional foods. •Build mechanistic
understanding on biomarkers and pathways relevant to cardiovascular diseases
and metabolic syndrome.
•Build network with the key players in the area.Professional Skills:
•Ph.D. or postdoc. in cardiovascular pharmacology or physiology is
essential |
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S***s
发帖数: 67 | 9
If interested, please contact Dr. Happy Li at h******[email protected]. Thanks.
=========================================================
Working location: Unilever R&D Shanghai
Job title: Assistant scientist or scientist
Contactperson: Dr. Happy Li (h******[email protected])
Description of main responsibilities
* Build up the toolbox mainly in vitro to identify actives with
cardiovascular benefits for functional foods.
* Build mechanistic understanding on biomarke |
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h*******o
发帖数: 4884 | 10 投journal of neuroscience被拒，
reviewer从根本上否决了这个study,
说data没问题，但是缺乏mechanistic investigation，导致novelty不够
这个study花了不少时间，不想直接就降到<4的杂志去了
打算找个差不多的再试试看
如果有直接推荐也可以
谢谢！ |
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h*******o
发帖数: 4884 | 11 AD的，用的AD的transgenic mouse模型，做了一些manipulation然后看treatment的作用
围绕着mitochondrial function展开的
结果reviewer就说这个没有新意，大家都知道mitochondrial function很重要， 文章
只是describe了treatment 对于 mitochondrial function的影响，但是没有
mechanistic investigation ，所以是describe the obvious, lack of novelty
通篇看下来没有任何postive的comments
只是这个实验光动物处理就花了半年，加上isolate fresh mitochondria再做assay,以
及后期的IHC和各种各样的实验。 而且还是建立在以前发现的基础上的，所有的
methodology都已经work out以后还花了1年多的时间
不太甘心就直接到4分的杂志了
确实neuroscience这一块，在JNS和neuroscience之间没什么杂志
老板说JNS现在提高rejection rat |
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h*******o
发帖数: 4884 | 12 My opinion
I assume it is a introductory/leading paragraph rather than a detailed
specific aim experiment design.
As a proposal, you dont' have to list the detailed methods in an
introductory paragraph. I would trim it a lot to something like,
To investigate the role of APPLE activation/dysregulation in APPLE
diseases, we propose to use a variety of biochemical, genetical and
analytical assays that summate at the mechanistic role of APPLE in APPLE
disease.
Proceed to expand the |
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n********k
发帖数: 2818 | 13 It is likely too late for a PHD or starting postdoc...I just found out last
night, a few of ideas I am planning on lncRNA have been worked OUT and just published in either cell, mole
cell etc...sure, there would be still a couple of ys to gold-rush in term of general mechanistic studies and
many ys ahead for functional dissections... and I am sure there are a lot more groups out there working on
lncRNA now... |
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c*********r
发帖数: 1312 | 14 你觉得noncoding RNA除了在基因表达调控方面的作用，会不会在结构方面也有作用？
last
just published in either cell, mole
term of general mechanistic studies and
more groups out there working on |
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w***r
发帖数: 709 | 15 解释一个现象，
阐明疾病的机理，
发明一个工具
比回答某个分子的功能
更适合喜欢思考的人。
last
just published in either cell, mole
term of general mechanistic studies and
more groups out there working on |
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h********n
发帖数: 4079 | 16 I think your point 1,2,3 are targeting the cancer stem cell research field,
not C Kim's work. I think you don't agree the concept of cancer stem cell.
I don't argue against you at this point because more data are required for
the field.
4. most lung cancer mouse models yield adenocarcinoma, including Kras and
EGFR model, which largely due to the promoter. And mechanistic study of
adenocarcinoma is much better then other subtype of lung cancers.
5. I have not read her recent work, but I was in h... 阅读全帖 |
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s**********e
发帖数: 2888 | 17 我博士导师需要马上招一个博后，非常非常nice的一个美国人。广告全文在下面，有兴
趣的可以直接联系他，位置是在UW-Madison，谢谢。
这个工作是部分延续我们实验室以前做的一部分化学的工作，这个位置是AICR的
funding资助做后面的机理工作，所以相关的文章很多还没有发表出来。有兴趣的可以
直接联系他，或者想知道实验室情况的也可以问我，谢谢。
我已经毕业了，我的博士老板是我这么多年遇到的最nice和对我事业帮助最大的人，所
以帮他把广告贴在这里。
Department of Food Science Research Associate opportunity
Post-doctoral Research Associate. Project involves evaluation of
biological activities related to anti-cancer effects of naturally occurring
organosulfur components, including metabolic fate and cell signaling ... 阅读全帖 |
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b**j
发帖数: 415 | 18 first one should make sure you have observed a solid phenomena (or phenotype
changes) or made a novel finding, then you want to work on the mechanistic
detail to make a story. Otherwise consider to change project or quit lab. |
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h*******n
发帖数: 2052 | 19 I cannot agree more!
phenotype
mechanistic |
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a********k
发帖数: 2273 | 20 fish eats fly/worm？ 说实话没觉得。basic science的话最多同等level，因为没有
mechanistic insight。。。筛药另当别论 |
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n***3
发帖数: 663 | 21 Thanks! But I prefer a oncogenesis lab with mechanistic studies, not so
interested in drug design.
of |
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R*******d
发帖数: 13640 | 22 agree.
"mechanistic insights" "limited". |
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g****1
发帖数: 261 | 23
yes, with just circumstances evidence of "mechanistic insights", this
type of journal will not accept for publication. This is more clear cut
rejection than you think.
Either pin down more direct mechanism then re-submit, or move down to the
next journal. |
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j***z
发帖数: 105 | 24 Is 5% of p53 expression under normal condition or after DNA damage induction
? Is it mRNA expression or protein expression? This could give important
insights into mechanistic study.
By the way, if you want to get a good paper out of this, patient data is key
. Get some tumor samples and measure the expression level of your tumor
supressor. Correlate with prognosis etc... |
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D*****l
发帖数: 554 | 25 Thank you for your suggestions.Your questions are answered in the following.
Is 5% of p53 expression under normal condition or after DNA damage induction
? Is it mRNA expression or protein expression? This could give important
insights into mechanistic study.
Without any treatment, p53 protein level in KO mouse skin is downregulated
to 5%---50% level of wt mouse skin.
By the way, if you want to get a good paper out of this, patient data is key
Get some tumor samples and measure the expression le... 阅读全帖 |
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c*****t
发帖数: 198 | 26 Title: The Paradigm of G Protein Receptor Transactivation: A Mechanistic
Definition and Novel Example
Authors: Little, Peter J. ; Burch, Micah L; Al-aryahi, Sefaa ; Zheng,
Wenhua
Journal: TheScientificWorldJOURNAL
Year: 2011
Volume: 11
Page Range: 709-714
www.thescientificworld.com/TSW/toc/TSWJ_ArticleLanding.asp?
ArticleId=3724&jid=
Thank you very much. |
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h********n
发帖数: 4079 | 27 1) I don't know. There is no solid data about cell phone radiation's
physiologic affect.
2) I don't know either.
I guess the simple experiment is to give cultured cell line radiation (mimic
those of cell phone) for months or longer and see any effect. I guess some
people did it but did not find anything interesting.
epi is not the only thing we can look at, but currently epi data is the data
we can have. No mechanistic research. On the other hand, for smoking,
there are solid evidence: if y... 阅读全帖 |
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t**u
发帖数: 7 | 28 帮朋友贴一个---
Postdoctoral position for Microcell Mediated Chromosome Transfer in Sloan
Kettering Institution, New York, NY.Department of Cancer Biology and
Genetics Center for Cell Engineering Sloan Kettering Institute, New York
Affiliated with Weill Medical College of Cornell University
Looking for individual who has a prior research experience on microcell
mediated chromosome transfer (MMCT).
The positions will focus on mechanistic approaches to understand 1)
reprogramming of somatic cells to plu... 阅读全帖 |
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g****y
发帖数: 26 | 29 【 以下文字转载自 Postdoc 讨论区 】
发信人: galupy (Galoppogos-horse), 信区: Postdoc
标 题: postdoc opening@Sloan-Kettering Institute
关键字: postdoc opening,sloan kettering
发信站: BBS 未名空间站 (Sun Aug 14 14:28:02 2011, 美东)
We are seeking highly motivated individuals for postdoctoral positions in
the Structural Biology Program of the Sloan-Kettering Institute, Memorial
Sloan-Kettering Cancer Center, NYC, USA.
The lab develops cutting-edge techniques (Nature,doi:10.1038/ nature09163 (
2010)) aimed at gaining a mechanisti... 阅读全帖 |
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s******y
发帖数: 28562 | 30 不甘心啊，
我们觉得这个文章质量很好，从某种意义上来讲比我上一片还好（但不是同一个题材，
所以新意是没有问题的），大致上就是发现了某个领域里有一个被大家忽略了很久的东
西有重要的作用。我们的文章里把它的功能基本上搞清楚了，调控网络也大致定了一个
大概方向。但是现在主编们都异口同声的说我们的文章没有mechanistic insight. 我
晕，一个几乎从来没有人做过的东西，怎么可以第一篇文章就指望我把详细mechanism
全部搞清楚？我把功能都已经搞清楚了，这还不够么？
这种东西发到太低的杂志，未必就讨好。因为主要是特别新而已，要是说具体的分子机
制的话，我们这个文章反而没有，不见得就一定能过reviewer 的关。这种亏我们实验
室别人吃过的，就是一开始他投到一个比较低的杂志去，被骂得狗血淋头天旋地转的回
来了，后来一生气投到了一个很好的杂志去，反而很顺利的被接受了，还被杂志写了篇
mini-review 来表扬其“重要意义”。
而且要是发到太低的杂志还会产生一个大问题，就是以后的follow-up 肯定会比第一篇
的档次更低。这种情况对于小实验室很常见，比方说第一篇发到了JBC... 阅读全帖 |
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s******y
发帖数: 28562 | 31 不是，我们的题目是 A Novel xxx modification of YYY plays an essential role in ZZZ
然后主编说，你们这个文章居然起这个一个题目in the absence of mechanistic insights...
打击得我几乎都爬不起来了。。。 |
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g****1
发帖数: 261 | 32
in ZZZ
insights...
without more details, reading the title, I expect to see mechanistic
insights in the paper :) |
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s******y
发帖数: 28562 | 33 啊。。。那就挂不上边了：（
至少目前还挂不上边，因为目前还仅仅是做基础的。不过将来肯定是挂得上的，
pathway B有一个后继follow-up 就是和免疫有关的。
看来我要不就咬咬牙把"mechanistic insight" 给做了得了。
因为看来大家对这个的意见都挺统一的，那就是不做这个就不够说服力，那么我就豁出
去了做一做吧。 |
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f*******7
发帖数: 1019 | 34 说不定你可以用这些要做的mechanistic insight写个R01 |
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s******y
发帖数: 28562 | 35 从来没有人报道过那个蛋白和pathway B有关，我在没有相关文章发表的情况下去申请
，是肯定拿不到funding的。
其实相关数据我曾经拿过去去申请过两次AHA的奖学金，每次被批说我胡说八道。
我现在得仔细想想，挑一个最重要的mechanistic insight做出来尽快把这个文章发了。 |
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s******y
发帖数: 28562 | 36 说到这个mechanistic insight,
昨晚我痛定思痛的想了一下，我大概可以做这几个方面：
１。提供该蛋白和pathway B具体某个蛋白的作用证据，这个我其实很粗的做过一些初
步试验，那个蛋白在被修饰之后貌似增强了pathway B里面一个重要成分的活性。当然
这个实验很粗糙，所以这个数据就没有包含到投才出去的文章去。
我可以好好想想，找一个比较好的assay 来证明一下。这个应该不是特别难做，就是会
费点时间。因为相关蛋白的提取和assay很花时间.
但这个如果做出来的话，应该算是比较直接的证据了吧？
２。提供证据说明为什么那个蛋白被修饰之前不能和pathway B 作用，修饰之后就可以
了。比分说是否修饰之后就对pathway A affinity 低了，对pathway B affinity 高了。
但是这个可能会比较复杂，因为pathway A, B 虽然细胞内定位有区别，但是有好几个
公用的蛋白成分，而且都是很大的complex, 还涉及膜什么的，不太可能用简单的co-IP.
我想对于这篇文章，这个大概是可以先搁着不做的吧？ |
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g****1
发帖数: 261 | 37
50%
be
recently
drug
It's true that identification of drug target has been a very difficult
challenge for several decades. The current drug development process of
screening compounds and testing in animal and human without truly
understanding the "mechanistic insight" hasn't been working well. Whoever
develops a good method to find small molecule target is going to become
famous. |
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s******y
发帖数: 28562 | 38 呵呵，可能我讲的不是很详细吧。
你说的就是我想说的。
我的意思就是不要把中医整体作为一个垃圾堆丢弃，而是要像看旧paper 一样，
从里面寻找有用信息，对于关键数据要用新方法证实一下，但是没有必要完全
推倒从头建一个数据系统。比方说假如70年代的人说他们用某个抑制剂之后
细胞会怎么怎么样，那个时候是肯定没有分子通道这个概念的，所以他们的文章
也是几乎没有"mechanistic insight" 的。但不等于说他们测的这个抑制剂的效果
就是肯定是完全错的，虽然我们可能会由我们的已知知识出发来用更仔细更多control
的条件来测，来推导其中的详细机理。 |
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s******y
发帖数: 28562 | 39 HiFa driven promoter 不是一个好marker, 因为后面要做mechanistic insight 的话
，肯定要针对HiFa 做一些事情。所以用这个做marker 的话会对后继试验带来麻烦。 |
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n*********b
发帖数: 140 | 40 Yes, the others include Owen Witte and Baltimore for their work on the
mechanistic insight of the ABL family.
Between Rowley's discovery and their work, there was a discovery of the BCR-
ABL fusion transcript that has clearly revealed the presence of the BCR-ABL
chimera protein. If Nobel goes to cancer genetics, these work may not be
credited together.
If Nobel goes to the illustration of posttranslational modification in
signal transduction or other aspects of physiology and medicine, their ... 阅读全帖 |
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R***t
发帖数: 14 | 41 代友发帖
～～～
Postdoctoral Research Fellow in Cancer Immunology, Medical University of
South Carolina, Charleston, SC, United States
Postdoctoral position is available in an exciting new laboratory to study
cancer immunology.
My lab is interested in cancer vaccine and innate immunity. My prior work
has been in the area of defining the roles of heat shock protein gp96 in
chaperoning TLRs (Liu et al. Nature Commun, 2010), understanding the roles
of gp96 in regulating hematopoiesis (Liu and Li, Blood 20... 阅读全帖 |
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w******n
发帖数: 371 | 42 标 题: 第一批CLS博士后基金入选者名单公示（清华博士后水准高）
发信站: 水木社区 (Sun Nov 27 11:44:48 2011), 站内
八位申请人拟入选CLS清华博士后基金，现将名单公示如下：
特等博士后基金：
1.龚蓉：2011年7月，在中国医学科学院北京协和医学院获得理学博士学位
2005年7月， 在武汉大学获得理学学士学位
代表性一作论文：
1.Rong Gong*, Ji Hu* Cheng Ding* et al. (2011). Role for the membrane
receptor guanylyl cyclase-C in attention deficiency and hyperactive behavior
. Science 333:1642-1646. (*equal authorship.)
2、梅子青：2009年12月，在清华大学获得理学博士学位
2002年7月， 在吉林大学获得理学硕士学位
1999年7月， 在吉林大学获得理学学士学位
... 阅读全帖 |
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y******8
发帖数: 1764 | 43 Single cell is the selling point.
Where is their mechanistic study?
Anyway, you don't need mechanism to publish on high-profile journals, if you
are doing fancy stuff. |
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h********n
发帖数: 4079 | 44 Mechanistic study is just a subset of biomedical research.
you |
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l**********1
发帖数: 5204 | 45 Stelle Pensum Veröffentlicht Deadline 15 04 2012 Referenz-
Nr. SAIS435
Postdoctoral Researcher in the field of Mechanistic modelling of the
toxicity of engineered nanoparticles towards aquatic organisms (PostDoc)
//internet1.refline.ch/673277/0105/++publications++/1/index.html
more
//www.telejob.ch/search/academics/environment/13
//www.telejob.ch/search/academics/list |
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w***r
发帖数: 709 | 46 No mechanistic insight right now, only detailed domain characterization.
I am not in plant field, but I don't think you could publish in journal IF>6
at the moment.
But I do think the question about evolution is interesting.
I hope this paper is helpful.
Science. 2010 Dec 17;330(6011):1682-5.
New genes in Drosophila quickly become essential.
Chen S, Zhang YE, Long M.
Abstract
To investigate the origin and evolution of essential genes, we identified
and phenotyped 195 young protein-coding genes, ... 阅读全帖 |
|
d****d
发帖数: 214 | 47 Editorial
Minimizing the “Re” in Review
Elizabeth H. Williams1,Pamela A. Carpentier2, andTom Misteli3
There is a troubling trend in scientific publishing for manuscripts to
undergo multiple, often lengthy, rounds of review, resulting in significant
delays to publication. JCB is announcing new procedures to streamline its
editorial process and eliminate unnecessary delays.
It is exceedingly rare that a manuscript is published as originally
submitted to a journal. Revisions are an integral par... 阅读全帖 |
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d****d
发帖数: 214 | 48 I think JCB is fully aware of Sunnyday's complain of the demand of "full
stories". Below is the part of the editorial related to this issue:
"Cases of prolonged review are often associated with another trend in
publishing: the requirement to present a “full story” for a paper to be
publishable. There can be tremendous value in simple observations that may
have the potential to open up new lines of investigation in a budding field
without providing all the mechanistic details. The quest for the f... 阅读全帖 |
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i**y
发帖数: 71 | 49 No particular reasons... I feel their works are relatively more mechanistic
than most other "neuro-epigenetists". Schier doesn't even study epigenetics
in the nervous system. He just happens to study both Rudolf Jaenisch is good
too, although he doesn't seem to work as much on epigenetics as before.
these |
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n********k
发帖数: 2818 | 50 I c, I agree both are very good scientifically and have vision too...u also
said, Schier actually is not in anyway in epigenetic field; Jerry runs hard
core epigenetic lab and has done beautiful and systematic work on the field
but he is really not a neural guy, nor Jaenisch..., as well, Jerry's later
work is more hot than systematic i would say...
mechanistic
epigenetics
good |
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